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Benthamiana Plants (benthamiana + plant)

Distribution by Scientific Domains
Life Sciences100%

Kinds of Benthamiana Plants

  • nicotiana benthamiana plant
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    Selected Abstracts

    Soil-Borne Wheat Mosaic Virus (SBWMV) 37 kDa Protein Rescues Cell-to-Cell and Long-Distance Movement of an Immobile Tobacco Mosaic Virus Mutant in Nicotiana benthamiana, a Non-Host of SBWMV

    JOURNAL OF PHYTOPATHOLOGY, Issue 1 2005
    C. Zhang
    Abstract To verify the role and examine the functional range of the 37 kDa putative movement protein (MP) of soil-borne wheat mosaic virus (SBWMV), the 37 kDa gene was inserted into an infectious tobacco mosaic virus (TMV)-based expression vector (p30B), to generate p30BMP. The 30 kDa cell-to-cell MP gene of TMV was then inactivated (in p30BMP to give p30B,MP) by a frameshift mutation which removed 80 amino acids from its C-terminus. Systemic infection of Nicotiana benthamiana plants occurred following inoculation with in vitro transcripts of p30BMP or p30B,MP. Progeny viral RNAs from inoculated and systemically infected leaves were analysed by reverse transcriptase polymerase chain reaction and ApaI digestion, and by sequencing. The 30 kDa TMV MP or its truncated form were detected, respectively, in Western blots of cell wall protein extracts from p30BMP-transcript or p30B,MP-transcript inoculated or systemically infected N. benthamiana leaves. High levels of SBWMV 37 kDa MP were detected in all cases. The results suggest that the 37 kDa protein of SBWMV, a monocotyledonous-infecting furovirus, can complement both cell-to-cell and long-distance movement functions in a defective heterologous virus (TMV) in N. benthamiana, a non-host of SBWMV. [source]

    SGT1 positively regulates the process of plant cell death during both compatible and incompatible plant,pathogen interactions

    MOLECULAR PLANT PATHOLOGY, Issue 5 2010
    KERI WANG
    SUMMARY SGT1 (suppressor of G2 allele of Skp1), an interactor of SCF (Skp1-Cullin-F-box) ubiquitin ligase complexes that mediate protein degradation, plays an important role at both G1,S and G2,M cell cycle transitions in yeast, and is highly conserved throughout eukaryotes. Plant SGT1 is required for both resistance (R) gene-mediated disease resistance and nonhost resistance to certain pathogens. Using virus-induced gene silencing (VIGS) in Nicotiana benthamiana, we demonstrate that SGT1 positively regulates the process of cell death during both host and nonhost interactions with various pathovars of Pseudomonas syringae. Silencing of NbSGT1 in N. benthamiana plants delays the induction of hypersensitive response (HR)-mediated cell death against nonhost pathogens and the development of disease-associated cell death caused by the host pathogen P. syringae pv. tabaci. Our results further demonstrate that NbSGT1 is required for Erwinia carotovora - and Sclerotinia sclerotiorum -induced disease-associated cell death. Overexpression of NbSGT1 in N. benthamiana accelerates the development of HR during R gene-mediated disease resistance and nonhost resistance. Our data also indicate that SGT1 is required for pathogen-induced cell death, but is not always necessary for the restriction of bacterial multiplication in planta. Therefore, we conclude that SGT1 is an essential component affecting the process of cell death during both compatible and incompatible plant,pathogen interactions. [source]

    Biochemical and immunological characterization of the plant-derived candidate human immunodeficiency virus type 1 mucosal vaccine CTB,MPR649,684

    PLANT BIOTECHNOLOGY JOURNAL, Issue 2 2009
    Nobuyuki Matoba
    Summary Plants are potentially the most economical platforms for the large-scale production of recombinant proteins. Thus, plant-based expression of subunit human immunodeficiency virus type 1 (HIV-1) vaccines provides an opportunity for their global use against the acquired immunodeficiency syndrome pandemic. CTB,MPR649,684[CTB, cholera toxin B subunit; MPR, membrane proximal (ectodomain) region of gp41] is an HIV-1 vaccine candidate that has been shown previously to induce antibodies that block a pathway of HIV-1 mucosal transmission. In this article, the molecular characterization of CTB,MPR649,684 expressed in transgenic Nicotiana benthamiana plants is reported. Virtually all of the CTB,MPR649,684 proteins expressed in the selected line were shown to have assembled into pentameric, GM1 ganglioside-binding complexes. Detailed biochemical analyses on the purified protein revealed that it was N- glycosylated, predominantly with high-mannose-type glycans (more than 75%), as predicted from a consensus asparagine,X,serine/threonine (Asn-X-Ser/Thr) N- glycosylation sequon on the CTB domain and an endoplasmic reticulum retention signal attached at the C-terminus of the fusion protein. Despite this modification, the plant-expressed protein retained the nanomolar affinity to GM1 ganglioside and the critical antigenicity of the MPR649,684 moiety. Furthermore, the protein induced mucosal and serum anti-MPR649,684 antibodies in mice after mucosal prime-systemic boost immunization. Our data indicate that plant-based expression can be a viable alternative for the production of this subunit HIV-1 vaccine candidate. [source]

    Mature monomeric forms of Hop stunt viroid resist RNA silencing in transgenic plants

    THE PLANT JOURNAL, Issue 6 2007
    G. Gómez
    Summary Viroids, small non-coding pathogenic RNAs, are able to induce RNA silencing, a phenomenon that has been associated with the pathogenesis and evolution of these small RNAs. It has been recently suggested that viroids may resist this plant defense mechanism. However, the simultaneous degradation of non-replicating full-length viroid RNA, and the resistance of mature forms of viroids to RNA silencing, have not been experimentally demonstrated. Transgenic Nicotiana benthamiana plants expressing a dimeric form of Hop stunt viroid (HSVd) that have the capability to cleave and circularize this viroid RNA were used to address this question. A reporter construct, consisting of a full-length HSVd RNA fused to GFP-mRNA, was agroinfiltrated in these plants and its expression was suppressed. Interestingly, both circular and linear HSVd molecules were stable and able to traffic through grafts in these restrictive conditions, indicating that the mature forms of HSVd are able, in some way, to resist the RNA-silencing mechanism. The observation that a full-length HSVd RNA fused to GFP-mRNA, but not circular and/or linear viroid forms, was fully susceptible to RNA degradation strongly suggests that structures adopted by the free mature monomer protect the pathogenesis-associated forms of the viroid from RNA silencing. [source]

    The conserved Xanthomonas campestris pv. vesicatoria effector protein XopX is a virulence factor and suppresses host defense in Nicotiana benthamiana

    THE PLANT JOURNAL, Issue 6 2005
    Matthew Metz
    Summary Nicotiana benthamiana leaves display a visible plant cell death response when infiltrated with a high titer inoculum of the non-host pathogen, Xanthomonas campestris pv. vesicatoria (Xcv). This visual phenotype was used to identify overlapping cosmid clones from a genomic cosmid library constructed from the Xcv strain, GM98-38. Individual cosmid clones from the Xcv library were conjugated into X. campestris pv. campestris (Xcc) and exconjugants were scored for an altered visual high titer inoculation response in N. benthamiana. The molecular characterization of the cosmid clones revealed that they contained a novel gene, xopX, that encodes a 74-kDa type III secretion system (TTSS) effector protein. Agrobacterium -mediated transient expression of XopX in N. benthamiana did not elicit the plant cell death response although detectable XopX protein was produced. Interestingly, the plant cell death response occurred when the xopX Agrobacterium -mediated transient expression construct was co-inoculated with strains of either Xcv,xopX or Xcc, both lacking xopX. The co-inoculation complementation of the plant cell death response also depends on whether the Xanthomonas strains contain an active TTSS. Transgenic 35S- xopX -expressing N. benthamiana plants also have the visible plant cell death response when inoculated with the non- xopX -expressing strains Xcv,xopX and Xcc. Unexpectedly, transgenic 35S- xopX N. benthamiana plants displayed enhanced susceptibility to bacterial growth of Xcc as well as other non- xopX -expressing Xanthomonas and Pseudomonas strains. This result is also consistent with the increase in bacterial growth on wild type N. benthamiana plants observed for Xcc when XopX is expressed in trans. Furthermore, XopX contributes to the virulence of Xcv on host pepper (Capsicum annuum) and tomato (Lycopersicum esculentum) plants. We propose that the XopX bacterial effector protein targets basic innate immunity in plants, resulting in enhanced plant disease susceptibility. [source]

    Plant-produced human growth hormone shows biological activity in a rat model

    BIOTECHNOLOGY PROGRESS, Issue 2 2009
    Shailaja Rabindran
    Abstract Plants have been shown to be efficient systems for expressing a wide range of recombinant proteins from various origins. Here, using a plant virus-based expression vector to produce human growth hormone (hGH) in Nicotiana benthamiana plants, we demonstrate, for the first time, that the plant-produced hGH (pphGH) is biologically active in a hypophysectomized rat model. We observed an average weight gain of ,17 g per animal in a group of 10 animals that were injected subcutaneously with pphGH with 60 ,g/dose for 10 days. With the increasing demand for hGH, accompanied with the need to make this recombinant protein available to a wider population at a more reasonable cost, plants provide a feasible alternative to current production platforms. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009 [source]