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Wide Variety (wide + variety)
Selected AbstractsAn Efficient and General Microwave-Assisted Copper-Catalyzed Conia-Ene Reaction of Terminal and Internal Alkynes Tethered to a Wide Variety of CarbonucleophilesADVANCED SYNTHESIS & CATALYSIS (PREVIOUSLY: JOURNAL FUER PRAKTISCHE CHEMIE), Issue 13 2010Sonia Montel Abstract This paper describes a highly efficient, microwave-assisted, Conia-ene reaction of alkynes bearing a stabilizing carbon nucleophile. The reaction, catalyzed by a commercially available copper catalyst, proceeds under neutral conditions and is generally applicable even to less reactive nucleophiles such as malonate, cyanoacetate, and sulfonylacetate derivatives. This copper-mediated cycloisomerization is also applicable to internal unactivated alkynes leading exclusively to the corresponding 5-membererd products having an E -olefinic chemistry. [source] ChemInform Abstract: Alkali Salt of L-Proline as an Efficient and Practical Catalyst for the Cyanosilylation of a Wide Variety of Carbonyl Compounds under Solvent-Free Conditions.CHEMINFORM, Issue 32 2009Zhi-Liang Shen Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a "Full Text" option. The original article is trackable via the "References" option. [source] An Efficient Approach for the Synthesis of N-1 Substituted HydantoinsEUROPEAN JOURNAL OF ORGANIC CHEMISTRY, Issue 11 2008Vinod Kumar Abstract An efficient three-step route for the synthesis of N - 1 alkyl/aryl-substituted hydantoins was developed from inexpensive commercially available substrates. The reaction of amines with cyanogen bromide takes place to give monoalkyl/aryl cyanamides. This on treatment with methyl bromoacetate in the presence of sodium hydride in tetrahydrofuran affords methyl N -cyano- N -alkyl/arylaminoacetate, which undergoes hydrolysis and cyclization in the presence of 50,% H2SO4 to afford N - 1 substituted hydantoins in very good-to-excellent yields. Wide varieties of final products having primary, secondary, tertiary, and aryl substituents at the N - 1 position were successfully synthesized by this method. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008) [source] Furstyling on angle-split shell texturesCOMPUTER ANIMATION AND VIRTUAL WORLDS (PREV: JNL OF VISUALISATION & COMPUTER ANIMATION), Issue 2-3 2009Bin Sheng Abstract This paper presents a new method for modeling and rendering fur with a wide variety of furstyles. We simulate virtual fur using shell textures,a multiple layers of textured slices for its generality and efficiency. As shell textures usually suffer from the inherent visual gap errors due to the uniform discretization nature, we present the angle-split shell textures (ASST) approach, which classifies the shell textures into different types with different numbers of texture layers, by splitting the angle space of the viewing angles between fur orientation and view direction. Our system can render the fur with biological patterns, and utilizes vector field and scalar field on ASST to control the geometric variations of the furry shape. Users can intuitively shape the fur by applying the combing, blowing, and interpolating effects in real time. Our approach is intuitive to implement without using complex data structures, with real-time performance for dynamic fur appearances. Copyright © 2009 John Wiley & Sons, Ltd. [source] Directable animation of elastic bodies with point-constraintsCOMPUTER ANIMATION AND VIRTUAL WORLDS (PREV: JNL OF VISUALISATION & COMPUTER ANIMATION), Issue 3-4 2008Ryo Kondo Abstract We propose a simple framework for making elastic body animation with point constraints. In general, a physics-based approach for constraint animation offers a variety of animations with physically correct realism, which are achieved by solving the equations of motion. However, in the digital animation industry, solving the equations of motion is an indirect path to creating more art-directed animations that maintain a plausible realism. Our algorithms provide animators a practical way to make elastic body animation with plausible realism, while effectively using point-constraints to offer directatorial control. The animation examples illustrate that our framework creates a wide variety of point-constraint animations of elastic objects with greater directability than existing methods. Copyright © 2008 John Wiley & Sons, Ltd. [source] Video completion and synthesisCOMPUTER ANIMATION AND VIRTUAL WORLDS (PREV: JNL OF VISUALISATION & COMPUTER ANIMATION), Issue 3-4 2008Chunxia Xiao Abstract This paper presents a new exemplar-based framework for video completion, allowing aesthetically pleasing completion of large space-time holes. We regard video completion as a discrete global optimization on a 3D graph embedded in the space-time video volume. We introduce a new objective function which enforces global spatio-temporal consistency among patches that fill the hole and surrounding it, in terms of both color similarity and motion similarity. The optimization is solved by a novel algorithm, called weighted priority belief propagation (BP), which alleviates the problems of slow convergence and intolerable storage size when using the standard BP. This objective function can also handle video texture synthesis by extending an input video texture to a larger texture region. Experiments on a wide variety of video examples with complex dynamic scenes demonstrate the advantages of our method over existing techniques: salient structures and motion information are much better restored. Copyright © 2008 John Wiley & Sons, Ltd. [source] NRC 3D imaging technology for museum and heritage applicationsCOMPUTER ANIMATION AND VIRTUAL WORLDS (PREV: JNL OF VISUALISATION & COMPUTER ANIMATION), Issue 3 2003J. Taylor Abstract The National Research Council of Canada (NRC) has developed and patented three high-resolution 3D imaging systems and processing algorithms which have been applied to a wide range of museum and heritage recording applications. The systems have been designed for different imaging applications and, in collaboration with a number of national and international museums and cultural agencies, have been used to scan a wide variety of objects and sites. The objective of this paper is to present a summary of the 3D technology and examples of its heritage applications. Copyright © 2003 John Wiley & Sons, Ltd. [source] A MATLAB toolbox for solving acid-base chemistry problems in environmental engineering applicationsCOMPUTER APPLICATIONS IN ENGINEERING EDUCATION, Issue 4 2005Chetan T. Goudar Abstract A MATLAB toolbox incorporating several computer programs has been developed in an attempt to automate laborious calculations in acid-base chemistry. Such calculations are routinely used in several environmental engineering applications including the design of wastewater treatment systems and for predicting contaminant fate and transport in the subsurface. The computer programs presented in this study do not replace student thinking involved in formulating the problem solving strategy but are merely tools that simplify the actual problem solving process. They encompass a wide variety of acid-base chemistry topics including equilibrium constant calculations, construction of distribution diagrams for mono and multiprotic systems, ionic strength and activity coefficient calculations, and buffer index calculations. All programs are characterized by an intuitive graphical user interface where the user supplies input information. Program outputs are either numerical or graphical depending upon the nature of the problem. The application of this approach to solving actual acid-base chemistry problems is illustrated by computing the pH and equilibrium composition of a 0.1 M Na2CO3 system at 30°C using several programs in the toolbox. As these programs simplify lengthy computations such as ionization fraction and activity coefficient calculations, it is hoped they will help bring more complicated problems to the environmental engineering classroom and enhance student understanding of important concepts that are applicable to real-world systems. The programs are available free of charge for academic use from the authors. © 2005 Wiley Periodicals, Inc. Comput Appl Eng Educ 13: 257,265, 2005; Published online in Wiley InterScience (www.interscience.wiley.com); DOI 10.1002/cae.20051 [source] Möbius Transformations For Global Intrinsic Symmetry AnalysisCOMPUTER GRAPHICS FORUM, Issue 5 2010Vladimir G. Kim The goal of our work is to develop an algorithm for automatic and robust detection of global intrinsic symmetries in 3D surface meshes. Our approach is based on two core observations. First, symmetry invariant point sets can be detected robustly using critical points of the Average Geodesic Distance (AGD) function. Second, intrinsic symmetries are self-isometries of surfaces and as such are contained in the low dimensional group of Möbius transformations. Based on these observations, we propose an algorithm that: 1) generates a set of symmetric points by detecting critical points of the AGD function, 2) enumerates small subsets of those feature points to generate candidate Möbius transformations, and 3) selects among those candidate Möbius transformations the one(s) that best map the surface onto itself. The main advantages of this algorithm stem from the stability of the AGD in predicting potential symmetric point features and the low dimensionality of the Möbius group for enumerating potential self-mappings. During experiments with a benchmark set of meshes augmented with human-specified symmetric correspondences, we find that the algorithm is able to find intrinsic symmetries for a wide variety of object types with moderate deviations from perfect symmetry. [source] Tangible Heritage: Production of Astrolabes on a Laser EngraverCOMPUTER GRAPHICS FORUM, Issue 8 2008G. Zotti I.3.5 [Computer Graphics]: Computational geometry and object modelling , geometric algorithms, languages and systems; I.3.8 [Computer Graphics]: Applications Abstract The astrolabe, an analog computing device, used to be the iconic instrument of astronomers during the Middle Ages. It allowed a multitude of operations of practical astronomy which were otherwise cumbersome to perform in an epoch when mathematics had apparently almost been forgotten. Usually made from wood or sheet metal, a few hundred instruments, mostly from brass, survived until today and are valuable museum showpieces. This paper explains a procedural modelling approach for the construction of the classical kinds of astrolabes, which allows a wide variety of applications from plain explanatory illustrations to three-dimensional (3D) models, and even the production of working physical astrolabes usable for public or classroom demonstrations. [source] Automatic Creation of Object Hierarchies for Radiosity ClusteringCOMPUTER GRAPHICS FORUM, Issue 4 2000Gordon Müller Using object clusters for hierarchical radiosity greatly improves the efficiency and thus usability of radiosity computations. By eliminating the quadratic starting phase very large scenes containing about 100k polygons can be handled efficiently. Although the main algorithm extends rather easily to using object clusters, the creation of ,good' object hierarchies is a difficult task both in terms of construction time and in the way how surfaces or objects are grouped to clusters. The quality of an object hierarchy for clustering depends on its ability to accurately simulate the hierarchy of the energy flow in a given scene. Additionally it should support visibility computations by providing efficient ray acceleration techniques. In this paper we will present a new approach of building hierarchies of object clusters. Our hybrid structuring algorithm provides accuracy and speed by combining a highly optimized bounding volume hierarchy together with uniform spatial subdivisions for nodes with regular object densities. The algorithm works without user intervention and is well suited for a wide variety of scenes. First results of using these hierarchies in a radiosity clustering environment are very promising and will be presented here. The combination of very deep hierarchies (we use a binary tree) together with an efficient ray acceleration structure shifts the computational effort away from form factor and visibility calculation towards accurately propagating the energy through the hierarchy. We will show how an efficient single pass gathering can be used to minimize traversal costs. [source] Scene Graph and Frame Update Algorithms for Smooth and Scalable 3D Visualization of Simulated Construction OperationsCOMPUTER-AIDED CIVIL AND INFRASTRUCTURE ENGINEERING, Issue 4 2002Vineet R. Kamat One of the prime reasons inhibiting the widespread use of discrete-event simulation in construction planning is the absence of appropriate visual communication tools. Visualizing modeled operations in 3D is arguably the best form of communicating the logic and the inner working of simulation models and can be of immense help in establishing the credibility of analyses. New software development technologies emerge at incredible rates that allow engineers and scientists to create novel, domain-specific applications. The authors capitalized on a computer graphics technology based on the concept of the scene graph to design and implement a general-purpose 3D visualization system that is simulation and CAD-software independent. This system, the Dynamic Construction Visualizer, enables realistic visualization of modeled construction operations and the resulting products and can be used in conjunction with a wide variety of simulation tools. This paper describes the scene graph architecture and the frame updating algorithms used in designing the Dynamic Construction Visualizer. [source] Spatial Information: Classification and Applications in Building DesignCOMPUTER-AIDED CIVIL AND INFRASTRUCTURE ENGINEERING, Issue 4 2002Tang-Hung Nguyen Physical properties of building components are usually represented in building data models by their three-dimensional geometry and topology,also called spatial information. While geometric data of building components can be manipulated and managed by a computer-aided design (CAD) interface, their spatial relations (or topological information) are conventionally represented in a manual fashion into data models. The manual data representation, however, is inherently a complex and challenging task due to the wide variety of spatial relationships. Thus, topological information should be classified and modeled in such a way that the required spatial data for a particular design task can be automatically retrieved. This paper attempts to identify and classify various topological information commonly used in building design and construction into more specific categories (e.g., adjacency, connection, containment, separation, and intersection) to support automatic deduction of the spatial information in a computer-based building design system. The paper also discusses typical applications of the topological relations to different design activities. Finally, the development of deduction algorithms and the proposed building design system are briefly described. [source] The embedded ion method: A new approach to the electrostatic description of crystal lattice effects in chemical shielding calculationsCONCEPTS IN MAGNETIC RESONANCE, Issue 5 2006Dirk Stueber Abstract The nuclear magnetic shielding anisotropy of NMR active nuclei is highly sensitive to the nuclear electronic environment. Hence, measurements of the nuclear magnetic shielding anisotropy represent a powerful tool in the elucidation of molecular structure for a wide variety of materials. Quantum mechanical ab initio nuclear magnetic shielding calculations effectively complement the experimental NMR data by revealing additional structural information. The accuracy and capacity of these calculations has been improved considerably in recent years. However, the inherent problem of the limitation in the size of the systems that may be studied due to the relatively demanding computational requirements largely remains. Accordingly, ab initio shielding calculations have been performed predominantly on isolated molecules, neglecting the molecular environment. This approach is sufficient for neutral nonpolar systems, but leads to serious errors in the shielding calculations on polar and ionic systems. Conducting ab initio shielding calculations on clusters of molecules (i.e., including the nearest neighbor interactions) has improved the accuracy of the calculations in many cases. Other methods of simulating crystal lattice effects in shielding calculations that have been developed include the electrostatic representation of the crystal lattice using point charge arrays, full ab initio methods, ab initio methods under periodic boundary conditions, and hybrid ab initio/molecular dynamics methods. The embedded ion method (EIM) discussed here follows the electrostatic approach. The method mimics the intermolecular and interionic interactions experienced by a subject molecule or cluster in a given crystal in quantum mechanical shielding calculations with a large finite, periodic, and self-consistent array of point charges. The point charge arrays in the EIM are generated using the Ewald summation method and embed the molecule or ion of interest for which the ab initio shielding calculations are performed. The accuracy with which the EIM reproduces experimental nuclear magnetic shift tensor principal values, the sensitivity of the EIM to the parameters defining the point charge arrays, as well as the strengths and limitations of the EIM in comparison with other methods that include crystal lattice effects in chemical shielding calculations, are presented. © 2006 Wiley Periodicals, Inc. Concepts Magn Reson Part A 28A: 347,368, 2006 [source] Performance comparison of checkpoint and recovery protocolsCONCURRENCY AND COMPUTATION: PRACTICE & EXPERIENCE, Issue 15 2003Himadri Sekhar Paul Abstract Checkpoint and rollback recovery is a well-known technique for providing fault tolerance to long-running distributed applications. Performance of a checkpoint and recovery protocol depends on the characteristics of the application and the system on which it runs. However, given an application and system environment, there is no easy way to identify which checkpoint and recovery protocol will be most suitable for it. Conventional approaches require implementing the application with all the protocols under consideration, running them on the desired system, and comparing their performances. This process can be very tedious and time consuming. This paper first presents the design and implementation of a simulation environment, distributed process simulation or dPSIM, which enables easy implementation and evaluation of checkpoint and recovery protocols. The tool enables the protocols to be simulated under a wide variety of application, system, and network characteristics. The paper then presents performance evaluation of five checkpoint and recovery protocols. These protocols are implemented and executed in dPSIM under different simulated application, system, and network characteristics. Copyright © 2003 John Wiley & Sons, Ltd. [source] Significance of Specimen Databases from Taxonomic Revisions for Estimating and Mapping the Global Species Diversity of Invertebrates and Repatriating Reliable Specimen DataCONSERVATION BIOLOGY, Issue 2 2004RUDOLF MEIER More specifically, we demonstrate for a specimen database assembled during a revision of the robber-fly genus Euscelidia (Asilidae, Diptera) how nonparametric species richness estimators (Chao1, incidence-based coverage estimator, second-order jackknife) can be used to (1) estimate global species diversity, (2) direct future collecting to areas that are undersampled and/or likely to be rich in new species, and (3) assess whether the plant-based global biodiversity hotspots of Myers et al. (2000) contain a significant proportion of invertebrates. During the revision of Euscelidia, the number of known species more than doubled, but estimation of species richness revealed that the true diversity of the genus was likely twice as high. The same techniques applied to subsamples of the data indicated that much of the unknown diversity will be found in the Oriental region. Assessing the validity of biodiversity hotspots for invertebrates is a formidable challenge because it is difficult to decide whether species are hotspot endemics, and lists of observed species dramatically underestimate true diversity. Lastly, conservation biologists need a specimen database analogous to GenBank for collecting specimen records. Such a database has a three-fold advantage over information obtained from digitized museum collections: (1) it is shown for Euscelidia that a large proportion of unrevised museum specimens are misidentified; (2) only the specimen lists in revisionary studies cover a wide variety of private and public collections; and (3) obtaining specimen records from revisions is cost-effective. Resumen:,Sostuvimos que los millones de registros de especimenes publicados en miles de revisiones taxonómicas en décadas anteriores son una fuente de información costo-efectiva de importancia crítica para la incorporación de invertebrados en decisiones de investigación y conservación. Más específicamente, para una base de datos de especimenes de moscas del género Euscelidia (Asilidae, Diptera) demostramos como se pueden utilizar estimadores no paramétricos de riqueza de especies (Chao 1, estimador de cobertura basado en incidencia, navaja de segundo orden) para (1) estimar la diversidad global de especies, (2) dirigir colecciones futuras a áreas que están sub-muestreadas y/o probablemente tengan especies nuevas y (3) evaluar si los sitios globales de importancia para la biodiversidad basados en plantas de Myers et al. (2000) contienen una proporción significativa de invertebrados. Durante la revisión de Euscelidia el número de especies conocidas fue más del doble, pero la estimación de riqueza de especies reveló que la diversidad real del género probablemente también era el doble. Las mismas técnicas aplicadas a las sub-muestras de datos indicaron que gran parte de la diversidad no conocida se encontrará en la Región Oriental. La evaluación de la validez de sitios de importancia para la biodiversidad de invertebrados es un reto formidable porque es difícil decidir si las especies son endémicas de esos sitios y si las listas de especies observadas subestiman dramáticamente la diversidad real. Finalmente, los biólogos de la conservación requieren de una base de datos de especimenes análoga a GenBank, para obtener registros de especimenes. Dicha base de datos tiene una triple ventaja sobre la información obtenida de colecciones de museos digitalizadas. (1) Se muestra para Euscelidia que una gran proporción de especimenes de museo no revisados están mal identificados. (2) Sólo las listas de especimenes en estudios de revisión cubren una amplia variedad de colecciones privadas y públicas. (3) La obtención de registros en revisiones es costo-efectiva. [source] Manganese cell labeling of murine hepatocytes using manganese(III)-transferrin,CONTRAST MEDIA & MOLECULAR IMAGING, Issue 3 2008Christopher H. Sotak Abstract Manganese(III)-transferrin [Mn(III),Tf] was investigated as a way to accomplish manganese-labeling of murine hepatocytes for MRI contrast. It is postulated that Mn(III),Tf can exploit the same transferrin-receptor-dependent and -independent metabolic pathways used by hepatocytes to transport the iron analog Fe(III),Tf. More specifically, it was investigated whether manganese delivered by transferrin could give MRI contrast in hepatocytes. Comparison of the T1 and T2 relaxation times of Mn(III),Tf and Fe(III),Tf over the same concentration range showed that the r1 relaxivities of the two metalloproteins are the same in vitro, with little contribution from paramagnetic enhancement. The degree of manganese cell labeling following incubation for 2,7,h in 31.5,µm Mn(III),Tf was comparable to that of hepatocytes incubated in 500,µm Mn2+ for 1,h. The intrinsic manganese tissue relaxivity between Mn(III),Tf-labeled and Mn2+ -labeled cells was found to be the same, consistent with Mn(III) being released from transferrin and reduced to Mn2+. For both treatment regimens, manganese uptake by hepatocytes appeared to saturate in the first 1,2,h of the incubation period and may explain why the efficiency of hepatocyte cell labeling by the two methods appeared to be comparable in spite of the ,16-fold difference in effective manganese concentration. Hepatocytes continuously released manganese, as detected by MRI, and this was the same for both Mn2+ - and Mn(III),Tf-labeled cells. Manganese release may be the result of normal hepatocyte function, much in the same way that hepatocytes excrete manganese into the bile in vivo. This approach exploits a biological process,namely receptor binding, endocytosis and endosomal acidification,to initiate the release of an MRI contrast agent, potentially conferring more specificity to the labeling process. The ubiquitous expression of transferrin receptors by eukaryotic cells should make Mn(III),Tf particularly useful for manganese labeling of a wide variety of cells both in culture and in vivo. Published in 2008 by John Wiley & Sons, Ltd. [source] Single-cell detection by gradient echo 9.4 T MRI: a parametric studyCONTRAST MEDIA & MOLECULAR IMAGING, Issue 4 2006P. Smirnov Abstract Recent studies have shown that cell migration can be monitored in vivo by magnetic resonance imaging after intracellular contrast agent incorporation. This is due to the dephasing effect on proton magnetization of the local magnetic field created by a labelled cell. Anionic iron oxide nanoparticles (AMNP) are among the most efficient and non-toxic contrast agents to be spontaneously taken up by a wide variety of cells. Here we measured the iron load and magnetization of HeLa tumour cells labelled with AMNP, as a function of the external magnetic field. High-resolution gradient echo 9.4,T MRI detected individual labelled cells, whereas spin echo sequences were poorly sensitive. We then conducted a systematic study in order to determine the gradient echo sequence parameters (echo time, cell magnetization and resolution) most suitable for in vivo identification of single cells. Copyright © 2006 John Wiley & Sons, Ltd. [source] Structure and function of AMP-activated protein kinaseACTA PHYSIOLOGICA, Issue 1 2009J. S. Oakhill Abstract AMP-activated protein kinase (AMPK) regulates metabolism in response to energy demand and supply. AMPK is activated in response to rises in intracellular AMP or calcium-mediated signalling and is responsible for phosphorylating a wide variety of substrates. Recent structural studies have revealed the architecture of the ,,, subunit interactions as well as the AMP binding pockets on the , subunit. The , catalytic domain (1,280) is autoinhibited by a C-terminal tail (313,335), which is proposed to interact with the small lobe of the catalytic domain by homology modelling with the MARK2 protein structure. Two direct activating drugs have been reported for AMPK, the thienopyridone compound A769662 and PTI, which may activate by distinct mechanisms. [source] The role of stakeholders in Sydney's green gamesCORPORATE SOCIAL RESPONSIBILITY AND ENVIRONMENTAL MANAGEMENT, Issue 3 2002Kate Kearins This paper focuses on the various roles played by stakeholders in the construction of Sydney Olympics as the Green Games. It draws material from the official website of the Sydney 2000 Olympic Games, the websites and other material made public by major stakeholders, and the considerable commentary generated by the greening efforts of the games' organizers and their many partners and critics. Sydney's ,Green Games' is shown to involve a wide variety of stakeholders in both its construction and deconstruction. Environmental groups both assisted in defining the agenda and, in retaining their independence, reserved the right to evaluate and publicly critique performance. It is argued that through the engagement of stakeholders, organizations can be expected to understand and elucidate the different dimensions of the environmental challenge they face,even though at times, as the Sydney ,Green Games' example shows, they may not fully meet these more exacting expectations, and the environmental groups themselves thus risk becoming compromised. Copyright © 2002 John Wiley & Sons, Ltd. and ERP Environment. [source] Liquid-based cytology for cervical screeningCYTOPATHOLOGY, Issue 6 2000N. Payne England and Wales' new National Institute for Clinical Excellence (NICE) has completed the first of its appraisals and issued guidance on a diagnostic technique rather than a therapeutic intervention.1 It was directed to examine the use of liquid-based cytology (LBC) for cervical screening and took evidence from a wide variety of sources. LBC is a new method of preparing cervical samples for cytological examination. Unlike the conventional ,smear' preparation it involves making a suspension of cells from the sample and this is used to produce a thin layer of cells on a slide. [source] Protein kinase A RII-like (R2D2) proteins exhibit differential localization and AKAP interaction,CYTOSKELETON, Issue 7 2008Amy E. Hanlon Newell Abstract A-kinase anchoring proteins (AKAPs) bind to protein kinase A (PKA) via an amphipathic helix domain that interacts with a dimerization/docking domain on the regulatory (R) subunit of PKA. Four other mammalian proteins (ROPN1, ASP, SP17, and CABYR) also contain a highly conserved RII dimerization/docking (R2D2) domain, suggesting all four proteins may interact with all AKAPs in a manner similar to RII. All four of these proteins were originally detected in the flagellum of mammalian sperm. In this report, we demonstrate that all four R2D2 proteins are expressed in a wide variety of tissues and three of the proteins SP17, CABYR, and ASP are located in motile cilia of human bronchus and fallopian tubes. In addition, we detect SP17 in primary cilia. We also provide evidence that ROPN1 and ASP bind to a variety of AKAPs and this interaction can be disrupted with anchoring inhibitor peptides. The interaction of SP17 and CABYR with AKAPs appears to be much more limited. None of the R2D2 proteins appears to bind cAMP, a fundamental characteristic of the regulatory subunits of PKA. These observations suggest that R2D2 proteins utilize docking interactions with AKAPs to accomplish their function of regulating cilia and flagella. Based on location, affinity for AKAPs and lack of affinity for cAMP, it appears that each R2D2 protein has a unique role in this process. Cell Motil. Cytoskeleton 2008. © 2008 Wiley-Liss, Inc. [source] Photodynamic Therapy for the Treatment of Cutaneous Neoplasia, Inflammatory Disorders, and PhotoagingDERMATOLOGIC SURGERY, Issue 5 2009EMILY TIERNEY MD BACKGROUND Photodynamic therapy (PDT) has demonstrated high efficacy, minimal side effects, and improved cosmetic outcome when used for the treatment of actinic keratoses (AK), basal cell carcinoma (BCC), squamous cell carcinoma, and photoaging. METHODS To review the literature on the use of PDT in dermatologic surgery using MEDLINE. RESULTS Published clinical studies using PDT in the treatment of AKs yield overall efficacy rates ranging from 50% to 71% with one treatment to as high as 88% to 90% with two or more treatments. For superficial BCC, initial clearance rates were 76% to 97%, and for Bowen's disease, initial clearance rates ranged from 72% to 94% overall. The use of PDT for photorejuvenation is a relatively new application of this technology, which has shown promise in improving the appearance of fine lines, pigmentary variation, and telangiectasias. CONCLUSIONS The advantages of photodynamic therapy include the capacity for noninvasive targeted therapy through topical application of aminolevulinic acid and methyl aminolevulinic acid, with outstanding cosmetic results. Although the theory behind the use of chemical photosensitizers and ultraviolet light to treat a wide variety of skin disorders is straightforward, the practical application of this technology is evolving. Additional research into the precise mechanisms of action for specific photosensitizers and optimal light sources will be highly beneficial to the advancement of this technology. [source] Modified Single-Sling Myocutaneous Island Pedicle Flap: Series of 61 ReconstructionsDERMATOLOGIC SURGERY, Issue 11 2008ANDREA WILLEY MD BACKGROUND Bilevel undermining above and below the transverse nasalis muscle in the construction of a myocutaneous island pedicle flap produces a bilateral or unilateral muscular sling with exceptional vascular supply for reconstruction of defects on the distal nose. We present further modification of the single-sling myocutaneous island pedicle flap that expands its application to a wide variety of nasal defects and further defines its usefulness in nasal reconstruction. METHODS A series of 61 consecutive myocutaneous island pedicle flap reconstructions performed after Mohs surgery between March 2005 and July 2006 are presented. Flap modifications are presented, and advantages and limitations are discussed. RESULTS Flap modifications introduce additional reach and rotational mobility to the flap that permit extension of the flap to defects on the nasal tip and distal ala. CONCLUSION Modifications of the bilevel approach to the single-sling nasalis myocutaneous island pedicle flap further define its practicality in nasal reconstruction and expand its application to a variety of nasal defects. [source] Sebaceous Carcinoma, Basal Cell Carcinoma, Trichoadenoma, Trichoblastoma, and Syringocystadenoma Papilliferum Arising within a Nevus SebaceusDERMATOLOGIC SURGERY, Issue 12p2 2004Christopher J. Miller MD Background. Nevus sebaceus has a well-documented potential to develop a wide variety of neoplasms of both epidermal and adnexal origins. It is highly unusual for more than three tumors to arise simultaneously within a single nevus sebaceus. Sebaceous carcinoma arising within a nevus sebaceus is a rare occurrence. Objective. The objective was to report the case of a patient with a nevus sebaceus that simultaneously developed five distinct neoplasms of epidermal and various adnexal origins and to report the fourth case of sebaceous carcinoma arising within a nevus sebaceus. Methods. A 45-year-old woman presented with a nevus sebaceusthat contained five separate neoplasms, including sebaceous carcinoma, basal cell carcinoma, trichoadenoma, trichoblastoma, and syringocystadenoma papilliferum. Results. Complete excision of the nevus sebaceus and the five tumors was performed. Systemic work-up showed no evidence of metastatic disease or association with Muir-Torre syndrome. Conclusion. This case report highlights the diverse neoplastic potential of nevus sebaceus and demonstrates the capacity of this hamartoma to develop aggressive tumors, such as sebaceous carcinoma. Prophylactic excision or at least close clinical surveillance for sudden development of new growths is warranted in all cases of nevus sebaceus. [source] ,Best Practice' Options for the Legal Recognition of Customary TenureDEVELOPMENT AND CHANGE, Issue 3 2005Daniel Fitzpatrick Is there a ,best practice' model for the legal recognition of customary tenure? If not, is it possible to identify the circumstances in which a particular model would be most appropriate? This article considers these questions in the light of economic theories of property rights, particularly as illustrated by the World Bank's 2003 land policy report. While these theories have their flaws, the underlying concept of tenure security allows a typological framework for developing legal responses to customary tenure. In particular, this article suggests that the nature and degree of State legal intervention in a customary land system should be determined by reference to the nature and causes of any tenure insecurity. This hypothesis is discussed by reference to a wide variety of legal examples from Africa, Papua New Guinea and the South Pacific. The objective is not to suggest that law determines resource governance outcomes in pluralist normative environments, but to improve the quality of legal interventions in order to assist customary groups to negotiate better forms of tenure security and access to resources. [source] Sox genes regulate type 2 collagen expression in avian neural crest cellsDEVELOPMENT GROWTH & DIFFERENTIATION, Issue 8 2006Takashi Suzuki Neural crest cells give rise to a wide variety of cell types, including cartilage cells in the cranium and neurons and glial cells in the peripheral nervous system. To examine the relationship of cartilage differentiation and neural crest differentiation, we examined the expression of Col2a1, which encodes type 2 collagen often used as a cartilage marker, and compared it with the expression of Sox transcription factor genes, which are involved in neural crest development and chondrogenesis. We found that Col2a1 is expressed in many neural crest-derived cell types along with combinations of Sox9, Sox10 and LSox5. Overexpression studies reveal the activation of Col2a1 expression by Sox9 and Sox10, and cross-regulation of these Sox genes. Luciferase assay indicates a direct activation of the Col2a1 enhancer/promoter both by Sox9 and Sox10, and this activation is further enhanced by cAMP-dependent kinase (PKA) signaling. Our study suggests that the regulatory mechanisms are similar in cartilage and neural crest differentiation. [source] Generalised and Particularistic Thinking in Policy Analysis and Practice: The Case of Governance Reform in South AfricaDEVELOPMENT POLICY REVIEW, Issue 3 2009Olle Frödin This article is concerned with the relationship between generalised and particularistic knowledge in the context of policy-making and policy analysis. It argues that it is problematic to assume that a reform model will generate similar outcomes across a wide variety of contexts. It presents a conceptual framework, including the concepts of transaction domain and domain consensus, that enables context-sensitive analyses. The argument is exemplified by South Africa's introduction in the 1990s of an Integrated Development Planning model, based on British reform experience and various international public-management models. With a case study of such planning in Lukhanji Municipality in the Eastern Cape Province, it illustrates how the conceptual framework may be used in policy research and analysis. [source] Divergent roles of the DEAD-box protein BS-PL10, the urochordate homologue of human DDX3 and DDX3Y proteins, in colony astogeny and ontogenyDEVELOPMENTAL DYNAMICS, Issue 6 2006Amalia Rosner Abstract Proteins of the highly conserved PL-10 (Ded1P) subfamily of DEAD-box family, participate in a wide variety of biological functions. However, the entire spectrum of their functions in both vertebrates and invertebrates is still unknown. Here, we isolated the Botryllus schlosseri (Urochordata) homologue, BS-PL10, revealing its distributions and functions in ontogeny and colony astogeny. In botryllid ascidians, the colony grows by increasing the number of modular units (each called a zooid) through a whole colony synchronized and weekly cyclical astogenic budding process (blastogenesis). At the level of the colony, both BS-PL10 mRNA and its protein (78 kDa) fluctuate in a weekly pattern that corresponds with the animal's blastogenic cycle, increasing from blastogenic stage A to blastogenic stage D. At the organ/module level, a sharp decline is revealed. Primary and secondary developing buds express high levels of BS-PL10 mRNA and protein at all blastogeneic stages. These levels are reduced four to nine times in the new set of functional zooids. This portrait of colony astogeny differed from its ontogeny. Oocytes and sperm cells express high levels of BS-PL10 protein only at early stages of development. Young embryos reveal background levels with increased expressions in some organs at more developed stages. Results reveal that higher levels of BS-PL10 mRNA and protein are characteristic to multipotent soma and germ cells, but patterns deviate between two populations of differentiating stem cells, the stem cells involved in weekly blastogenesis and stem cells involved in embryogenesis. Two types of experimental manipulations, zooidectomy and siRNA assays, have confirmed the importance of BS-PL10 for cell differentiation and organogenesis. BS-PL10 (phylogenetically matching the animal's position in the evolutionary tree), is the only member of this subfamily in B. schlosseri, featuring a wide range of biological activities, some of which represent pivotal roles. The surprising weekly cyclical expression and the participation in cell differentiation posit this molecule as a model system for studying PL10 protein subfamily. Developmental Dynamics 235:1508,1521, 2006. © 2006 Wiley-Liss, Inc. [source] Inhibitory deficits in tourette's syndromeDEVELOPMENTAL PSYCHOBIOLOGY, Issue 1 2008Emily R. Stern Abstract A developmental approach to the study of psychopathology can broaden understanding of a wide variety of complex psychological disorders. This article reviews research on Tourette's syndrome (TS), a developmental disorder characterized by unwanted motor and vocal tics. Over the past decade, knowledge of the neurobiology and pathophysiology of TS has progressed rapidly. The application of brain imaging techniques, primarily magnetic resonance imaging, to the study of Tourette's has increased knowledge of structural and functional deficits in brain areas associated with behavioral and psychological disturbances in the disorder. By reviewing some of this work, we will describe one way in which knowledge of brain function in TS has both informed and been informed by a developmental science approach. In particular, we will consider the extent to which the cognitive and emotional development of persons with TS may be affected by specific neurobiological characteristics of the disorder. © 2007 Wiley Periodicals, Inc. Dev Psychobiol 50: 9,18, 2008. [source] | ||||||||||||||||||||||||||||||||||