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Selected AbstractsMouse toxicity of Anabaena flos-aquae from Lake Dianchi, ChinaENVIRONMENTAL TOXICOLOGY, Issue 1 2009Xiaojie Pan Abstract Some species of the genera Anabaena can produce various kinds of cyanotoxins, which may pose risks to environment and human health. Anabaena has frequently been observed in eutrophic freshwater of China in recent years, but its toxicity has been reported only in a few studies. In the present study, the toxicity of an Anabaena flos-aquae strain isolated from Lake Dianchi was investigated. Acute toxicity testing was performed by mouse bioassay using crude extracts from the lyophilized cultures. The mice exposed to crude extracts showed visible symptoms of toxicity and died within 10,24 h of the injection. Serum biochemical parameters were evaluated by the use of commercial diagnostic kits. Significant alterations were found in the serum biochemical parameters: alkaline phosphatase (AKP), ,-glutamyl transpeptidase (,-GT), aspartate amino transferase (AST), alanine amino transferase (ALT), AST/ALT ratio, total protein content, albumin content, albumin/globulin (A/G) ratio, blood urea nitrogen (BUN), serum creatinine (Ssr), and total antioxidative capacity (T-AOC). Histopathological observations were carried out with hematoxylin and eosin (HE) stain under light microscope. Severe lesions were seen in the livers, kidneys, and lungs of the mice injected with crude extracts. The alterations of biochemical parameters were in a dose-dependent manner, and the severities of histological lesions were in the same manner. Based on biochemical and histological studies, this research firstly shows the presence of toxin-producing Anabaena species in Lake Dianchi and the toxic effects of its crude extracts on mammals. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2009. [source] DROUGHT STRESS: Comparative Time Course Action of the Foliar Applied Glycinebetaine, Salicylic Acid, Nitrous Oxide, Brassinosteroids and Spermine in Improving Drought Resistance of RiceJOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 5 2010M. Farooq Abstract Worldwide rice productivity is being threatened by increased endeavours of drought stress. Among the visible symptoms of drought stress, hampered water relations and disrupted cellular membrane functions are the most important. Exogenous use of polyamines (PAs), salicylic acid (SA), brassinosteroids (BRs), glycinebetaine (GB) and nitrous oxide (NO) can induce abiotic stresses tolerance in many crops. In this time course study, we appraised the comparative role of all these substances to improve the drought tolerance in rice (Oryza sativa L.) cultivar Super-Basmati. Plants were subjected to drought stress at four leaf stage (4 weeks after emergence) by maintaining soil moisture at 50 % of field capacity. Pre-optimized concentrations of GB (150 mg l,1), SA (100 mg l,1), NO (100 ,mol l,1 sodium nitroprusside as NO donor), BR (0.01 ,m 24-epibrassinolide) and spermine (Spm; 10 ,m) were foliar sprayed at five-leaf stage (5 weeks after emergence). There were two controls both receiving no foliar spray, viz. well watered (CK1) and drought stressed (CK2). There was substantial reduction in allometric response of rice, gas exchange and water relation attributes by drought stress. While drought stress enhanced the H2O2, malondialdehyde (MDA) and relative membrane permeability, foliar spray of all the chemicals improved growth possibly because of the improved carbon assimilation, enhanced synthesis of metabolites and maintenance of tissue water status. Simultaneous reduction in H2O2 and MDA production was also noted in the plants treated with these substances. Drought tolerance was sturdily associated with the greater tissue water potential, increased synthesis of metabolites and enhanced capacity of antioxidant system. Of all the chemicals, foliar spray with Spm was the most effective followed by BR. [source] Genotypic difference for the susceptibility of Japanese, Chinese and European pears to Venturia nashicola, the cause of scab on Asian pearsPLANT BREEDING, Issue 4 2008K. Abe Abstract Venturia nashicola, the cause of scab on Asian pears, is distinct from Venturia pirina, a causal fungus of European pear scab. Although scab caused by V. nashicola is one of the most serious diseases in the Japanese pear (Pyrus pyrifolia Nakai var. culta Nakai), information available regarding resistant breeding against V. nashicola is limited. In this study, 12 genotypes of Japanese pear, seven genotypes of Chinese pear (Pyrus ussuriensis Maxim.) and four genotypes of European pear (Pyrus communis L. var. sativa DC.) and/or their offspring were evaluated for susceptibility to V. nashicola with leaf and fruit inoculation tests. At 30,40 days after full bloom in their developmental stage, unfolded young leaves and fruit were inoculated with conidial suspensions of V. nashicola for each genotype, and the responses were rated at 30 days postinoculation for the inoculated leaves and at 42 days postinoculation for the inoculated fruits. No visible symptoms were found in European pear ,Bartlett' and ,La France' and their respective offspring ,290-36' and ,282-12', in the Japanese pear ,Kinchaku' and in the Chinese pears ,Cangxili' and ,Hongli'; these genotypes were evaluated as highly resistant to V. nashicola. Necrotic lesions without sporulation were observed in the Chinese pears ,Qiubaili', ,Manyuanxiang', ,Yuanbali' and ,Xiangyali', which were regarded as resistant. Sporulating lesions were formed on the other genotypes, such as the major Japanese pear cultivars ,Kosui' and ,Nijisseiki', which were regarded as susceptible. The response of inoculated leaves coincided well with that of inoculated fruit for each genotype. When the severity of scab symptoms on scab-susceptible genotypes was further rated with disease severity (DS) values, a genotypic difference was observed for overall DS values in a successive 2-year measurement among the susceptible genotypes. Based on the DS values of leaf and fruit scabs, the Japanese pears ,Niitaka', ,Shinko', ,Nijisseiki', ,Gold Nijisseiki', ,Osa Nijisseiki' and ,Shinsui' were considered to be less susceptible to V. nashicola than the typical susceptible cultivar ,Kosui'. [source] Essential role of the V-ATPase in male gametophyte developmentTHE PLANT JOURNAL, Issue 1 2005Jan Dettmer Summary Intracellular pH homeostasis is a prerequisite for biological processes and requires the action of proton pumps. The vacuolar H+ -ATPase (V-ATPase) is involved in regulating pH in endomembrane compartments of all eukaryotic cells. In plants, there is an additional endomembrane proton pump, H+ -pyrophosphatase (H+ -PPase). However, the relative roles of the two types of pumps in endomembrane acidification and energization of secondary active transport are unclear. Here, we show that a strong T-DNA insertion allele of VHA-A, the single copy gene encoding the catalytic subunit of the Arabidopsis V-ATPase, causes complete male and partial female gametophytic lethality. Severe changes in the morphology of Golgi stacks and Golgi-derived vesicles in male gametophytes are the first visible symptoms of cell degeneration leading to a failure to develop mature pollen. Similar effects on Golgi morphology were observed in pollen tubes when growth was blocked by Concanamycin A, a specific V-ATPase inhibitor. Taken together, our results suggests that V-ATPase function is essential for Golgi organization and development of the male gametophyte. [source] Studies on Colletotrichum acutatum and Greeneria uvicola: Two fungi associated with bunch rot of grapes in sub-tropical AustraliaAUSTRALIAN JOURNAL OF GRAPE AND WINE RESEARCH, Issue 1 2007CHRISTOPHER C. STEEL Abstract Vineyards in the Hunter Valley and Hastings Valley (sub-tropical NSW) were examined by unaided eye for visible symptoms of bunch rot diseases at berry maturity in 2003 (for a total of five varieties over seven vineyards). Grey mould (Botrytis cinerea) was evident in three vineyards, and ripe rot (Colletotrichum acutatum) was evident in four of those seven vineyards surveyed. Other bunch rots at six of the vineyards could not be readily identified by visual inspection. The incidence of fungi on grapevine reproductive structures (and potentially bunch-rot fungi) was then recorded for a Cabernet Sauvignon vineyard in the Hastings Valley during the 2004/5 and 2005/6 growing seasons, and outcomes are reported here in some detail. By berry maturity, C. acutatum and Greeneria uvicola (bitter rot) were the predominant pathogens isolated from those structures, and constitute the first such report of bitter rot fungi on wine grapes in Australia. Indeed, the frequency of latent infection by C. acutatum and G. uvicola increased with berry development during the growing season. Other fungi isolated included Alternaria spp., Botryosphaeria spp., Cladosporium spp., Epicoccum sp., Fusarium spp., Nigrospora spp., Pestalotia spp., Phomopsis viticola and Trichoderma spp. Isolation of B. cinerea from this vineyard was rare. Infection of various wine grape varieties in vitro with C. acutatum and G. uvicola at post veraison revealed all varieties to be susceptible over a range of temperatures (20,35oC). Based on laboratory studies, there was no infection of berries at a relative humidity (RH) < 50%, and infection diminished at 87% RH. Infection did occur if the berries were first incubated at 100% RH for 24 hours, and then transferred to an environment of lower humidity. [source] Activation of defence reactions in Solanaceae: where is the specificity?CELLULAR MICROBIOLOGY, Issue 1 2007Sabine Desender Summary When a potential pathogen attempts to infect a plant, biochemical and molecular communication takes place and leads to the induction of plant defence mechanisms. In the case of efficient defence, visible symptoms are restricted and the pathogen does not multiply (incompatible interaction); when defence is inefficient, the plant becomes rapidly infected (compatible interaction). During the last 30 years, a growing body of knowledge on plant,pathogen interactions has been gathered, and a large number of studies investigate the induction of various plant defence reactions by pathogens or by pathogen-derived compounds. However, as most papers focus on incompatible interactions, there is still a lack of understanding about the similarities and differences between compatible and incompatible situations. This review targets the question of specificity in Solanaceae,pathogen interactions, by comparing defence patterns in plants challenged with virulent or avirulent pathogens (or with pathogen-associated molecular patterns from these). A special emphasis is made on analysing whether defence reactions in Solanaceae depend primarily on the type of elicitor, on the plant genotype/species, or on the type of interaction (compatible or incompatible). [source] |