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Very High Concentrations (very + high_concentration)
Selected AbstractsVariations of chemical compositions in coarse aerosols and fine aerosols in two successive episodesENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 8 2006Chung-Yih Kuo Abstract Particulate matter with diameters less than 2.5 ,m (PM2.5) and ranging between 10 to 2.5 ,m (PM10-2.5) were simultaneously collected at four air-quality monitoring stations in the Taichung area of central Taiwan during the period of February 12 to 22, 2004. Two different types of PM10 episodes, a nonlocal dust-storm episode and a local episode, were observed in the present study. High concentrations of coarse aerosols occurred during the dust-storm episode, whereas high concentrations of fine aerosols were present during the local episode. Relatively high levels of Na+, Mg2+, Ca2+, and Cl, in coarse aerosols were observed during the dust-storm episode. Very high concentrations of secondary aerosols (NH+4, SO2,4, and NO,3) in fine aerosols were observed during the local episode. The nitrate ion demonstrated the greatest increase in the ratios of ionic species to PM2.5 and ionic species to PM10-2.5 during the local episode. Significantly high ratios (0.444) of NO,3 to NO2 in fine aerosols were present during the local episode, indicating that the relatively high formation rate of NO,3 was one of the important factors leading to the increase of the NO,3 to PM2.5 ratio during the local episode. Results also showed that an abundant quantity of fine ammonium nitrate was formed during the local episode, and chloride depletion probably was the major pathway to form coarse NaNO3 during this episode. [source] An overview of the microphysical structure of cirrus clouds observed during EMERALD-1THE QUARTERLY JOURNAL OF THE ROYAL METEOROLOGICAL SOCIETY, Issue 607 2005M. W. Gallagher Abstract High-resolution ice microphysical, turbulence, heat and water vapour flux data in cirrus clouds were collected by the Airborne Research Australia's (ARA) Egret Grob 520T research aircraft during the first Egret Microphysics with Extended Radiation and Lidar experiment (EMERALD-1). The in situ cirrus measurements were guided by simultaneous airborne lidar measurements collected by the ARA Super King Air research aircraft which flew below the cirrus and whose horizontal position was synchronized with the Egret. This allowed the microphysics and turbulence measurements to be interpreted and evaluated within the context of large-scale cirrus structure and its evolution. A significant feature of the clouds observed was the presence on occasion of active convective columns. Large variations in the cirrus dynamics were observed, with significant variations in the ice crystal habit from cloud top to cloud base and within the evaporating fall-streaks of precipitation. However, on average the picture presented is consistent with that shown by Heymsfield and Miloshevich, and by Kajikawa and Heymsfield, with the upper supersaturated region of the cloud acting as an active particle-generation zone where homogeneous nucleation proceeds apace; ice crystals there are initially dominated by small irregular or spheroidally shaped particles, some of which can be identified as proto or ,germ' rosettes. These are then observed to grow into more open bullet rosette and columnar types as they fall into the less supersaturated middle and lower layers of the cloud. The mean recognisable ice particle size fell within a very narrow size band, 70,90 µm, but the actual size distribution is thought to increase in a continuous manner to smaller sizes. However, there are currently instrument limitations that make it difficult to confirm this unambiguously. Unlike most previous studies, however, the cirrus clouds observed here were mostly devoid of pristine plate-like crystals, as nucleation and growth within the planar growth regime was rarely encountered. During some cases bullet rosettes, once formed, did undergo transition to the plate growth regime with complex crystal shapes resulting. The mean size of pristine bullet rosettes was again confined to a relatively narrow range. The likely nucleation processes dominating in cirrus clouds are discussed in the light of the observations. Very high concentrations of small ice crystals were sometimes detected, concentrations reaching a maximum of 10 000 L,1. There is strong evidence supporting these high concentrations which are probably produced by the homogeneous freezing of aerosol. Copyright © 2005 Royal Meteorological Society [source] Speciation and Environmental Fate of Chromium in Rivers Contaminated with Tannery EffluentsENGINEERING IN LIFE SCIENCES (ELECTRONIC), Issue 2 2007J. Dominik Abstract Redox and size speciation of chromium in rivers contaminated with tannery wastewater was carried out to provide insight into its transport and removal mechanisms. Total chromium was determined with Inductively Coupled Plasma-Mass Spectrometry and Cr,(VI) with Catalytic Adsorption Stripping Voltammetry. For the size speciation, particles were retained with a cartridge filter (cut-off 1.2,,m) and the total filterable fraction was further fractionated with Tangential Flow Filtration to determine the concentrations of chromium associated with the High Molecular Weight Colloidal (HMWC), Low Molecular Weight Colloidal (LMWC) and Truly Dissolved (TD) fractions. Two fluvial systems of similar sizes, but located in contrasting climatic zones, were selected for comparison: the Sebou-Fez system in Morocco and Dunajec River-Czorsztyn Reservoir system in Poland. Particulate Cr dominated in the Sebou-Fez system (about 90,%); while in the Dunajec-Czorsztyn system, it represented only 17,53,% of the total chromium in raw water. Still, the partition coefficients [Kd] were of the same magnitude. Chromium,(III) was the only form detected in Sebou-Fez, whereas in Dunajec-Czorsztyn Cr,(VI) was also present with its proportion increasing downstream from the input of tannery wastewater due to the preferential removal of Cr,(III). In the filtered water in Morocco a large fraction of Cr occurred in the HMWC fraction (50,70,%) at the two most contaminated sites, while the LMWC and TD forms prevailed at the non-contaminated sites in the Sebou River. At a very high concentration, in the water in the proximity of tanneries (well above the theoretical saturation level) Cr precipitated as polynuclear Cr-hydroxide. In Dunajec-Czorsztyn, the partition of Cr,(III) was approximately equal between the HMWC, LMWC and TD fractions, in contrast to Cr,(VI) which occurred almost exclusively in the TD fraction. In both systems, Cr,(III) was rapidly removed from the water to the sediments. The confluence of the Sebou with the Fez and the Czorsztyn reservoir trapped efficiently Cr,(III) preventing its spreading over long distances. Cr,(VI) showed conservative behavior and bypassed the Czorsztyn Reservoir. This study provides a first set of data on the partitioning of Cr,(III) and Cr,(VI) between the particulate, the colloidal and truly dissolved fractions in fluvial systems contaminated with tannery effluents. It also suggests that, in these systems, truly dissolved Cr,(III) can be adequately modeled from the total filterable concentrations. [source] Phytotoxicity and phytoaccumulation of trivalent and hexavalent chromium in brake fernENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 8 2005Yi Su Abstract A recently recognized hyperaccumulator plant, Chinese brake fern (Pteris vittata), has been found to extract very high concentration of arsenic from arsenic-contaminated soil. Chromium usually is a coexisting contaminant with arsenic in most contaminated soils. The potential application of ferns for phytoremediation of chromium(III)- and chromium(VI)-contaminated soils and their phytotoxicity to ferns has not been studied before. In this study, chromium distribution and phytotoxicity at the plant and cellular levels of brake ferns were studied using chemical analyses and scanning electron microscopy. The results show a higher phytotoxicity of Cr from Cr(VI)-contaminated soil to Chinese brake fern than from Cr(III)-contaminated soil. Phytotoxicity symptoms included significant decreases both in fresh biomass weight and relative water content (RWC), and also in leaf chlorosis during the late stage of growing. At higher concentrations (500 mg/kg Cr[VI] and 1,000 mg/kg Cr[III] addition), plants showed reduction in the number of palisade and spongy parenchyma cells in leaves. Compared with other plant species reported for phytoremediation of Cr(VI)-contaminated soil, brake fern took up and accumulated significant amounts of Cr (up to 1,145 mg/kg in shoots and 5,717 mg/kg in roots) and did not die immediately from phytotoxicity. Our study suggests that Chinese brake fern is a potential candidate for phytoremediation of Cr(VI)-contaminated soils, even though plants showed severe phytotoxic symptoms at higher soil Cr concentrations. [source] In situ polymerization of polyethylene/clay nanocomposites using a novel clay-supported Ziegler-Natta catalystPOLYMER COMPOSITES, Issue 10 2009Ahmad Ramazani S.A. Polyethylene/clay nanocomposites (PECNC) were synthesized via in situ Ziegler-Natta catalyst polymerization. Activated catalyst for polymerization of ethylene monomer has been prepared at first by supporting of the cocatalyst on the montmorillonite (MMT) smectite type clay and then active complex for polymerization formed by reaction of TiCl4 and aluminum oxide compound on the clay. Acid wash treatment has been used for increasing hydroxyl group and porosity of the clay and subsequently activity of the catalyst. The nanostructure of composites was investigated by X-ray diffraction (XRD) and transmission electron microscopy (TEM). Obtained results show that silica layers of the mineral clay in these polyethylene/nanocomposites were exfoliated, intercalated, and uniformly dispersed in the polyethylene matrix even at very high concentration of the clay. Thermogravimetric analysis (TGA) shows good thermal stability of the PECNCs. Differential scanning calorimeter (DSC) results reveal considerable decrease in the crystalline phase of the PECNC samples. Results of permeability analysis show an increase in barrier properties of PECNC films. POLYM. COMPOS., 2009. © 2009 Society of Plastics Engineers [source] Geochronology and Geochemistry of Mafic Dikes from Hainan Island and Tectonic ImplicationsACTA GEOLOGICA SINICA (ENGLISH EDITION), Issue 6 2009CAO Jianjin Abstract: In the present study, the major and trace element compositions, as well as Sr, Nd isotopic compositions and K-Ar age data in mafic dikes from Hainan Island, China, have been analyzed. Whole-rock K-Ar dating yielded a magmatic duration of 61,98 Ma for mafic dikes. Mafic dikes have a very high concentration of incompatible elements, for example, Ba, Rb, Sr, K, rare earth elements, and especially light rare earth elements (LREE), and negative anomalies of Nb, Ta, and Ti in the normalized trace element patterns. The initial 87Sr/86Sr ratios and ,Sr(t) of the mafic dikes are 0.70634,0.71193 and +27.7 to +112.2, respectively. In the 87Sr/86Sr versus ,Nd(t) diagram, the Hainan Island mafic dikes plot between fields for depleted mantle and enriched mantle type 2. All these characteristics show that the mantle (source region) of mafic dikes in this area experienced metasomatism by fluids relatively enriched in LREE and large ion lithophile elements. The genesis of Hainan Island mafic dikes is explained as a result of the mixing of asthenospheric mantle with lithospheric mantle that experienced metasomatism by the subduction of the Pacific Plate. This is different from the Hainan Island Cenozoic basalts mainly derived from depleted asthenospheric mantle, and possibly, minor metasomatised lithospheric mantle. This study suggests that the Mesozoic and Cenozoic lithospheric revolutions in Hainan Island can be divided into three stages: (1) the compression orogenesis stage before 98 Ma. The dominant factor during this stage is the subduction of the ancient Pacific Plate beneath this area. The lithospheric mantle changed into enriched mantle type 2 by metasomatism; (2) the thinning and extension stage during 61,98 Ma. The dominant factor during this stage is that the asthenospheric mantle invaded and corroded the lithospheric mantle; and (3) the large-scale thinning and extension stage after 61 Ma. The large-scale asthenospheric upwelling results in the strong erupting of Cenozoic basalts, large-scale thinning of the lithosphere, the southward translating and counterclockwise rotating of Hainan Island, and the opening of the South China Sea. [source] Reproducible protein analysis by CE using linear polyacrylamide-coated capillaries and hydrochloric acid rinsingELECTROPHORESIS, Issue 13 2007Adhitasari Suratman Abstract Hydrochloric acid was investigated as a rinsing reagent to remove adsorbed proteins from linear polyacrylamide-coated capillaries for electrophoresis. Three model proteins were used, namely cytochrome c as a basic protein, ,-lactoglobulin as an acidic protein, and ,-casein as a more easily denaturing protein. In order to regenerate capillary surfaces, they have been rinsed for 5,min with 2,M hydrochloric acid, 5,min with water, and then 30,min with buffer after every tenth run. It was found important to perform this regeneration procedure on time. The obtained results show good repeatability of the apparent EOF mobility with percentage RSDs below 3% (n,=,60) in various cases. These good results were mainly confirmed in long-term series with more than 200 runs each. Only very high concentrations (175,,M) of ,-lactoglobulin and ,-casein at pH,3.5 gave RSD% values above 5%. For these conditions, the further test of 85% m/m phosphoric acid as rinsing reagent showed a good repeatability of the apparent EOF mobilities as well. [source] A century of the synthesis of dapsone: its anti-infective capacity now and thenINTERNATIONAL JOURNAL OF DERMATOLOGY, Issue 10 2000Ronni Wolf MD Background Although dapsone was first synthesized in 1908, a quarter of a century was to pass before it was used in the treatment of bacterial infections. Dapsone was, however, too toxic for humans (because of the excess dosage which was administered at that time) and was thus considered to be of no value in the treatment of common bacterial infections. Since the early 1950s, dapsone has been recognized as being uniquely effective against a number of noninfectious, inflammatory diseases and, today, this is its main indication. Thus, the reason why dapsone was first introduced into medicine, namely the treatment of bacterial infections, has been set aside and its main current applications are the treatment of noninfectious, inflammatory, autoimmune, and bullous diseases. Objective To study the anti-infective capacity of dapsone against common bacterial infections. As many patients who receive dapsone for the treatment of noninfectious, inflammatory diseases have a concomitant bacterial infection or a superinfection of their skin disease, we thought that, if dapsone proved to be effective against common bacterial infections, it may obviate the need for an additional antimicrobial drug in these patients. Methods Three bacterial ATCC> strains (Streptococcus pyogenes, Staphylococcus aureus, and Escherichia coli) were tested by a macrodilution minimal inhibitory concentration (MIC) test for dapsone. Dapsone concentrations were between 0.06 and 1125 ,g/mL. Results Even the highest concentration of dapsone of 1125 ,g/mL did not inhibit bacterial growth. Conclusions Our results indicate that dapsone has no antibacterial effects whatsoever. Even at very high concentrations, it does not suppress the growth of most susceptible strains of bacteria. The story of dapsone (i.e. the long time that elapsed between its synthesis to its use for the chemotherapy of infectious diseases) will not repeat itself this time. [source] Organic acids: old metabolites, new themesJOURNAL OF CHEMICAL TECHNOLOGY & BIOTECHNOLOGY, Issue 10 2006Israel Goldberg Abstract Fumaric, L -malic and citric acids are intermediates of the oxidative tricarboxylic acid (TCA) cycle which in eukaryotes is localized in mitochondria. These organic acids are synthesized and accumulated in the medium to very high concentrations by filamentous fungi such as Aspergillus spp. and Rhizopus sp. This article reviews basic research on the unusual acid production capability and the associated metabolic pathways operating under defined stress conditions in these specific fungi. In particular, we describe and discuss the importance of the cytosolic reductive TCA pathway, which includes the cytosolic activities of pyruvate carboxylase, malate dehydrogenase and fumarase, for production of fumaric and L -malic acids. This article also describes the differences between fumaric acid, L -malic acid and citric acid production by different organisms (filamentous fungi, yeast, and higher eukaryotes), and the possible application of novel technologies (genetic engineering and bioinformatics) to fungal systems which may offer new industrial potential of filamentous fungi for the production of valuable metabolites. Copyright © 2006 Society of Chemical Industry [source] Lysosomal sequestration of amine-containing drugs: Analysis and therapeutic implicationsJOURNAL OF PHARMACEUTICAL SCIENCES, Issue 4 2007Allyn M. Kaufmann Abstract Amine-containing drugs represent a very important class of therapeutic agents, with the majority of all drugs containing at least one basic nitrogen. For many decades, it has been known that weakly basic compounds can be sequestered into acidic organelles such as lysosomes. Some amines can achieve very high concentrations and induce a dramatic expansion (vacuolization) of the compartment. In the early 70s, Nobel laureate and discoverer of lysosomes, Christian de Duve et al. wrote an elegant commentary describing the theoretical basis for lysosomal sequestration of amines, referring to the process as pH-partitioning and the substrates as lysosomotropics. Recently, a resurgence of interest in the intracellular distribution of drugs has occurred considering its therapeutic importance. Specifically, lysosomal sequestration of amines has received considerable attention for reasons including its involvement in drug resistance, inducement of phospholipidosis, and its influence on whole body distribution/pharmacokinetics. Moreover, the sequestration phenomenon has been recently exploited in the development of a novel drug targeting strategy. This review will focus on these occurrences/developments and conclude with a commentary on the expected impact that knowledge regarding the intracellular distribution of drugs will likely have on future drug development processes. © 2006 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 96: 729,746, 2007 [source] Characterization of a bacteriocin produced by Prevotella nigrescens ATCC 25261LETTERS IN APPLIED MICROBIOLOGY, Issue 5 2004J. Kaewsrichan Abstract Aims:, To characterize the antimicrobial activity produced by Prevotella nigrescens ATCC 25261, and to evaluate its safety on cultured gingival fibroblasts. Methods and Results:, An antimicrobial activity was obtained from purifying the culture supernatant of Pr. nigrescens ATCC 25261. Purification of the active compound was achieved with ammonium sulphate precipitation followed by anion-exchange and gel filtration chromatography. As revealed by SDS-PAGE, the active fraction was relatively homogeneous, showing a protein with an approximate molecular weight of 41 kDa. The antimicrobial compound, named nigrescin, exhibited a bactericidal mode of action against Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, and Actinomyces spp. Nigrescin was stable in a pH range between 6·5 and 9·5, at 100°C for 10 min, and resistant to lyophilization. But its activity was lost after proteinase K treatment. Despite at very high concentrations beyond the minimum inhibitory concentration (MIC), nigrescin was not toxic to the gingival fibroblasts. Conclusion:, Nigrescin is a novel bacteriocin produced by Pr. nigrescens ATCC 25261. It exhibits antimicrobial activity against species that are implicated in periodontal diseases. The absence of toxicity on the gingival fibroblasts suggests the possibility in using of nigrescin for an application in periodontal treatment. Significance and Impact of the Study:, Novel evidence on nigrescin would make Pr. nigrescens ATCC 25261 attractive in biotechnological applications as an antimicrobial agent in clinical dentistry. [source] Seabed Morphology and Pollution Along the Bagnoli Coast (Naples, Italy): a Hypothesis for Environmental RestorationMARINE ECOLOGY, Issue 2002Tommaso De Pippo Abstract. The interaction of coastal and submarine morphology with the hydrodynamic regimes exerts a control on coastal dynamic processes, conditioning the dispersion of sediments and potential pollutants existing in the area. Thus, the study of such parameters is useful in order to define environmental threats. Within the submerged sector of the Bagnoli coast and particularly in the southern part of the investigated area, there are sediment groups composed of very fine sands located in low-energy zones due to wave refraction and diffraction; they can also be found on the sea bottom and on the man-made structures typical of this zone. These areas show high pollutant levels of N, P, Cu, Fe, Mn, Ni, Pb, Zn, Cd, polyaromatic hydrocarbons (PAHs) and polychlorinated bi-phenyls (PCBs). The northern area, a place of high-energy hydrodynamic processes, also shows high concentration levels of pollutants due to the presence of secondary cell circulation. Morphological research and analysis of textural characteristics of bottom sediments along the Bagnoli coast allowed the actual processes and their evolution in space and time to be defined. It has also been possible to correlate such processes to the seabed morphology system, the wave formations which affect the coast, the complex system of sediment transport, as well as to the man-made interventions in the area. The results of recent chemical analyses of beach sediments and bottom sediments off the Bagnoli coast were also incorporated. They prove the presence of heavy metals, PAHs and PCBs in high and sometimes very high concentrations. Finally, based on the results of research and analyses, a hypothesis for an intervention for environmental restoration has been formulated in order to renaturalise the coast through dredging and treatment of the sand, both on the seabed and on the emerged beach. [source] Absent reduction by HIV protease inhibitors of Candida albicans adhesion to endothelial cellsMYCOSES, Issue 3 2007Barbara Falkensammer Summary Highly active antiretroviral therapy including HIV protease inhibitors has led to a marked reduction of clinically relevant mucosal candidiasis. We have previously shown that HIV protease inhibitors directly inhibit adhesion of Candida albicans to epithelial cells at concentrations that are reached in vivo during antiretroviral therapy. The aim of this study was to establish whether HIV protease inhibitors also inhibit adhesion of Candida to endothelial cells, which play a major role in systemic fungal disease. Three C. albicans strains were incubated with human umbilical vein endothelial cells or an endothelial cell line in the presence of either Ritonavir, Saquinavir or Indinavir. Subsequently, adherence was determined by counting colony-forming units. The results were comparable and revealed that Ritonavir and Saquinavir significantly inhibited adherence to endothelial cells at only very high concentrations which are likely not reached in vivo, and Indinavir did not even inhibit then. Inhibition of adhesion of C. albicans to human cells by HIV protease inhibitors is not a general feature, but strongly cell type-dependent, and clearly not observed for endothelial cells in vitro, which are a main target of systemic candidiasis in vivo. [source] Osteoprotegerin in the Inner Ear May Inhibit Bone Remodeling in the Otic Capsule,THE LARYNGOSCOPE, Issue 1 2005Andreas F. Zehnder MD Abstract Objectives: To elucidate factors that may be responsible for the inhibition of remodeling of bone within the otic capsule. Methods: Expression of osteoprotegerin (OPG), receptor activator of nuclear factor kappa B (RANK), and RANK ligand (RANKL) were assayed in samples of bone obtained from the otic capsule, calvarium, and femur, and from the soft tissue within the cochlea using semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) in mice. Immunostaining was used for histologic localization of the gene products. An enzyme-linked immunosorbent assay (ELISA) was used to quantify the amount of OPG within perilymph, serum, and cerebrospinal fluid. The micro-anatomy of the interface between the otic capsule and the fluid spaces of the cochlea was investigated by brightfield and phase-contrast microscopy and by three-dimensional reconstruction in the mouse and human. Results: OPG, a powerful inhibitor of bone remodeling, was expressed at extremely high levels within the soft tissue of the cochlea and was present in the perilymph at very high concentrations. The OPG produced within the inner ear may diffuse into the surrounding otic capsule, where it may be responsible for inhibition of bone turnover. Our anatomic studies revealed an extensive system of interconnected canaliculi within the otic capsule that had direct openings into the fluid spaces of the inner ear, thus providing a possible anatomic route for the diffusion of OPG from the inner ear into the surrounding bone. Conclusion: OPG, a potent inhibitor of osteoclast formation and function, is expressed at high levels within the inner ear and is secreted into the perilymph and the surrounding bone and may serve to inhibit active bone remodeling within the otic capsule, especially immediately adjacent to the cochlea. By this means, the cochlear soft tissue may control the nature of the surrounding petrous bone. [source] | |||||||||||||||||||