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Vertical Profiles (vertical + profile)

Distribution by Scientific Domains

Earth and Environmental Science	35%
Life Sciences	30%
Engineering	23%
3 Other Domains	12%

Selected Abstracts

Buckled Bridges Using Film Stress for Three-Dimensional Structures: Effects of Lateral Designs on Vertical Profiles and Dynamic Characteristics

IEEJ TRANSACTIONS ON ELECTRICAL AND ELECTRONIC ENGINEERING, Issue 2 2010
Minoru Sasaki Member
Abstract Bridges buckled by film stress can generate a vertical displacement resulting in three-dimensional structures. We have demonstrated a micromirror lifted by buckled bridges and a vertical comb drive actuator. The structures show rounded profiles in a stable manner. The detailed profiles of the bridges and the lifted micromirrors are examined. The relations between the lateral design and its effect on the vertical profile and the dynamic characteristics are studied. Copyright © 2010 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc. [source]

Vertical profiles of methanogenesis and methanogens in two contrasting acidic peatlands in central New York State, USA

ENVIRONMENTAL MICROBIOLOGY, Issue 8 2006
Hinsby Cadillo-Quiroz
Summary Northern acidic peatlands are important sources of atmospheric methane, yet the methanogens in them are poorly characterized. We examined methanogenic activities and methanogen populations at different depths in two peatlands, McLean bog (MB) and Chicago bog (CB). Both have acidic (pH 3.5,4.5) peat soils, but the pH of the deeper layers of CB is near-neutral, reflecting its previous existence as a neutral-pH fen. Acetotrophic and hydrogenotrophic methanogenesis could be stimulated in upper samples from both bogs, and phylotypes of methanogens using H2/CO2 (Methanomicrobiales) or acetate (Methanosarcinales) were identified in 16S rRNA gene clone libraries and by terminal restriction fragment length polymorphism (T-RFLP) analyses using a novel primer/restriction enzyme set that we developed. Particularly dominant in the upper layers was a clade in the Methanomicrobiales, called E2 here and the R10 or fen group elsewhere, estimated by quantitative polymerase chain reaction to be present at ,108 cells per gram of dry peat. Methanogenic activity was considerably lower in deeper samples from both bogs. The methanogen populations detected by T-RFLP in deeper portions of MB were mainly E2 and the uncultured euryarchaeal rice cluster (RC)-II group, whereas populations in the less acidic CB deep layers were considerably different, and included a Methanomicrobiales clade we call E1-E1,, as well as RC-I, RC-II, marine benthic group D, and a new cluster that we call the subaqueous cluster. E2 was barely detectable in the deeper samples from CB, further evidence for the associations of most organisms in this group with acidic habitats. [source]

The challenge of tree height in Eucalyptus regnans: when xylem tapering overcomes hydraulic resistance

NEW PHYTOLOGIST, Issue 4 2010
Giai Petit
Summary ,Recent research suggests that increasing conduit tapering progressively reduces hydraulic constraints caused by tree height. Here, we tested this hypothesis using the tallest hardwood species, Eucalyptus regnans. ,Vertical profiles of conduit dimensions and vessel density were measured for three mature trees of height 47, 51 and 63 m. ,Mean hydraulic diameter (Dh) increased rapidly from the tree apex to the point of crown insertion, with the greatest degree of tapering yet reported (b > 0.33). Conduit tapering was such that most of the total resistance was found close to the apex (82,93% within the first 1 m of stem) and the path length effect was reduced by a factor of 2000. Vessel density (VD) declined from the apex to the base of each tree, with scaling parameters being similar for all trees (a = 4.6; b = ,0.5). ,Eucalyptus regnans has evolved a novel xylem design that ensures a high hydraulic efficiency. This feature enables the species to grow quickly to heights of 50,60 m, beyond the maximum height of most other hardwood trees. [source]

Phylogenetic analyses of ribosomal DNA-containing bacterioplankton genome fragments from a 4000 m vertical profile in the North Pacific Subtropical Gyre

ENVIRONMENTAL MICROBIOLOGY, Issue 9 2008
Vinh D. Pham
Summary High-throughput identification of rRNA gene-containing clones in large insert metagenomic libraries is difficult, because of the high background of host ribosomal RNA (rRNA) and rRNA genes. To address this challenge, a membrane hybridization method was developed to identify all bacterial small subunit rRNA-containing fosmid clones of microbial community DNA from seven different depths in the North Pacific Subtropical Gyre. Out of 101,376 clones screened, 751 rDNA-containing clones were identified that grouped in ,60 different clades. Several rare sequences only remotely related to known groups were detected, including a Wolbachia -related sequence containing a putative intron or intervening sequence, as well as seven sequences from Order Myxococcales not previously detected in pelagic habitats. Stratified, depth-specific population structure was evident within both cultured and uncultured lineages. Conversely, some eurybathyal members of the genera Alcanivorax and Rhizobium shared identical small subunit ribosomal DNA sequences that were distributed from surface waters to the 4000 m depth. Comparison with similar analyses in Monterey Bay microbial communities revealed previously recognized, as well as some distinctive, depth-stratified partitioning that distinguished coastal from open ocean bacterioplankton populations. While some bias was evident in fosmid clone recovery in a few particular lineages, the overall phylogenetic group recovery and distributions were consistent with previous studies, as well as with direct shotgun sequence data from the same source DNA. [source]

Laboratory experimental check of a conceptual model for infiltration under complex rainfall patterns

HYDROLOGICAL PROCESSES, Issue 3 2006
Florisa Melone
Abstract Experimental evidence of the accuracy of the model proposed by Corradini et al. (1997, Journal of Hydrology192: 104,124) for local infiltration,redistribution,reinfiltration in homogeneous soils is given. The model provides infiltration through the time evolution of the soil water content vertical profile, which is described by an ordinary differential equation in any stage of a given rainfall event. A nearly horizontal laboratory slope was used for the experiments performed over both a medium- and a coarse-textured soil. During each experiment characterized by a complex rainfall pattern, the soil water content , at different depths was continuously monitored using the time-domain reflectometry method. Our results indicate that the model simulated the experimental vertical profiles of , accurately, particularly during the infiltration and reinfiltration stages separated by a rainfall hiatus with redistribution of soil water. These results indicate the reliability of the model in computing the local effective rainfall for hydrological response. Copyright © 2005 John Wiley & Sons, Ltd. [source]

Buckled Bridges Using Film Stress for Three-Dimensional Structures: Effects of Lateral Designs on Vertical Profiles and Dynamic Characteristics

Exploration of a Standing Mesochannel System with Antimatter/Matter Atomic Probes,

ADVANCED MATERIALS, Issue 24 2008
Hiroyuki K. M. Tanaka
Positronium, a system consisting of an electron and its antimatter, a positron, offers a new technique to explore vertical accessibility and connectivity. Here, we show how this technique can be used to map out the vertical profile of mesoporous silica channel systems by comparing a standing (perpendicular to the substrate) 2D hexagonal with a lying (parallel to the substrate) 2D hexagonal mesoporous film. [source]

Seasonal variability in the response of the airflow characteristics to the changes in the macro-scale westerly flow intensity over Europe, 1971,2000

INTERNATIONAL JOURNAL OF CLIMATOLOGY, Issue 4 2009
Michal Marosz
Abstract The aim of the research was to identify the seasonal variability of correlation between the air motion characteristics and common zonal circulation index (Rossby's Index). Air flow characteristics comprised divergence and vorticity. The spatial coverage of the research was the so called Euro-Atlantic Region covering parts of North Atlantic and Europe (40W,40E, 35N,75N). Temporal extent was 1971,2000 thus matching the latest WMO normal period. The data used was the components of the wind vector (u,zonal component, v,meridional component) and geopotential heights (hgt) from NCEP/NCAR Reanalysis. The response of the vorticity field is apparent and the greatest variability is noted at 1000 hPa. The mid and upper levels in the troposphere reveal quite homogenous response of vorticity to the macro-scale forcing. The response of the divergence field is also apparent though more complex in the vertical profile of the troposphere. The analysis of correlation was followed by the description of annual as well as seasonal shape of vorticity and divergence fields in the extremes of Rossby Index. Copyright © 2008 Royal Meteorological Society [source]

Direct analysis of sulfate reducing bacterial communities in gas hydrate-impacted marine sediments by PCR,DGGE

JOURNAL OF BASIC MICROBIOLOGY, Issue S1 2009
Christopher E. Bagwell
Abstract Molecular investigations of the sulfate reducing bacteria that target the dissimilatory sulfite-reductase subunit A gene (dsr A) are plagued by the nonspecific performance of conventional PCR primers. Here we describe the incorporation of the FailSafeÔ PCR System to optimize environmental analysis of dsr A by PCR amplification and denaturing gradient gel electrophoresis. PCR,DGGE analysis of dsr A composition revealed that SRB diversity was greater and more variable throughout the vertical profile of a marine sediment core obtained from a gas hydrate site (GC234) in the Gulf of Mexico than in a sediment core collected from a nearby site devoid of gas hydrates (NBP). Depth profiled dsr B abundance corresponded with sulfate reduction rates at both sites, though measurements were higher at GC234. This study exemplifies the numerical and functional importance of sulfate reducing bacteria in deep-sea sedimentary environments, and incremental methodological advancements, as described herein, will continue to streamline the analysis of sulfate reducer communities in situ. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source]

OBSERVATION WELL NETWORK DESIGN FOR PUMPING TESTS IN UNCONFINED AQUIFERS,

JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION, Issue 1 2003
Xunhong Chen
ABSTRACT: This paper presents a method for determining locations of observation wells to be used in conducting pumping tests in unconfined aquifers. Sensitivity coefficients, the distribution of relative errors, and the correlation coefficients between four aquifer parameters (horizontal and vertical hydraulic conductivities Kr and Kz, storage coefficient S, and specific yield Sy) are used as the criteria for the design of observation well networks and the interpretation of pumping tests. The contours of the relative errors over a vertical profile are very useful in selecting the "best" location of an observation well. Results from theoretical analyses suggest that a wide range of locations is suitable for the determination of Kr and that good locations for the determination of Kz and S may be poorly suited for the determination of Sy. Consideration must be given to the position and lengths of the pumping well screen in the selection of observation well locations. For a given location, the quality of test data can be improved by using high pumping rates and frequent sampling of drawdowns. We found that a minimum of two and preferably three observation locations are needed along a given transect. Results of the four parameters from a single well analysis may contain higher uncertainties. However, composite analyses of multiple observation wells can reduce the correlation between the four aquifer parameters, particularly between Kr and Sy, thus, improving the quality of parameter estimation. Results from two pumping tests conducted at sites located in Nebraska were examined with regard to the proposed methodology. [source]

Processing of turbulent-layer wind speed with Generalized SCIDAR through wavelet analysis

MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 4 2006
B. García-Lorenzo
ABSTRACT We describe a new method involving wavelet transforms for deriving the wind velocity associated with atmospheric turbulent layers from Generalized SCIDAR measurements. The algorithm analyses the cross-correlation of a series of scintillation patterns separated by lapses of ,t, 2,t, 3,t, 4,t and 5,t using wavelet transforms. Wavelet analysis provides the position, direction and altitude of the different turbulent layers detected in each cross-correlation. The comparison and consistency of the turbulent-layer displacements in consecutive cross-correlations allow the determination of their velocities and avoid misidentifications associated with noise and/or overlapping layers. To validate the algorithm, we have compared the velocity of turbulent layers derived on four nights with the wind vertical profile provided by balloon measurements. The software is fully automated and is able to analyse huge amounts of Generalized SCIDAR measurements. [source]

Determination of the profile of atmospheric optical turbulence strength from SLODAR data

MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 2 2006
T. Butterley
ABSTRACT Slope Detection and Ranging (SLODAR) is a technique for the measurement of the vertical profile of atmospheric optical turbulence strength. Its main applications are astronomical site characterization and real-time optimization of imaging with adaptive optical correction. The turbulence profile is recovered from the cross-covariance of the slope of the optical phase aberration for a double star source, measured at the telescope with a wavefront sensor (WFS). Here, we determine the theoretical response of a SLODAR system based on a Shack,Hartmann WFS to a thin turbulent layer at a given altitude, and also as a function of the spatial power spectral index of the optical phase aberrations. Recovery of the turbulence profile via fitting of these theoretical response functions is explored. The limiting resolution in altitude of the instrument and the statistical uncertainty of the measured profiles are discussed. We examine the measurement of the total integrated turbulence strength (the seeing) from the WFS data and, by subtraction, the fractional contribution from all turbulence above the maximum altitude for direct sensing of the instrument. We take into account the effects of noise in the measurement of wavefront slopes from centroids and the form of the spatial structure function of the atmospheric optical aberrations. [source]

Modeling and Simulation of Notional Future Radar in Non-Standard Propagation Environments Facilitated by Mesoscale Numerical Weather Prediction Modeling

NAVAL ENGINEERS JOURNAL, Issue 4 2008
ROBERT E. MARSHALL
Normal near surface radio-frequency (RF) propagation in the littorals across the land,sea boundary is rare due to the land,sea temperature difference, coastline shape, ground cover, urban density, coastal topography, and soil moisture content. The resulting frequent existence of coastal non-standard vertical profiles of refractivity and the resulting RF propagation has a profound impact on the performance of Navy ship-borne radars operating within 100 nm of the shore. In addition, these non-standard RF propagation conditions are spatio-temporally inhomogeneous. These spatial and time dependent propagation conditions and the resulting radar engineering implications cannot be revealed by a single vertical profile of refractivity taken near the ship borne radar. The results from single profile analysis techniques provide no spatiotemporal information and may lead to overly conservative radar design. Mesoscale numerical weather prediction (NWP) is a rapidly maturing technology with a strong operational Navy history that can provide a vertical profile of refractivity every 1 km in the battle space and every hour, up to 48 h, in the future. The Sensor Division at NSWCDD has applied mesoscale NWP for the last 2 years to better understand the performance of prototype radar in realistic four-dimensional (4D) coastal environments. Modern RF parabolic equation models are designed to model specific radar designs and to employ 3D refractivity fields from mesoscale NWP models. This allows for a radar design to be tested in realistic littoral non-standard atmospheres produced by stable internal boundary layers, sea breeze events, and the more rare sub-refractive events. Mesoscale NWP is currently qualitative for this purpose, but a research and development program focused on sea testing of prototype radars is described with the purpose of developing a more quantitative mesoscale NWP technology to support radar acquisition, testing, and operations. [source]

Acclimation of photosynthetic capacity to irradiance in tree canopies in relation to leaf nitrogen concentration and leaf mass per unit area

PLANT CELL & ENVIRONMENT, Issue 3 2002
P. Meir
Abstract The observation of acclimation in leaf photosynthetic capacity to differences in growth irradiance has been widely used as support for a hypothesis that enables a simplification of some soil-vegetation-atmosphere transfer (SVAT) photosynthesis models. The acclimation hypothesis requires that relative leaf nitrogen concentration declines with relative irradiance from the top of a canopy to the bottom, in 1 : 1 proportion. In combination with a light transmission model it enables a simple estimate of the vertical profile in leaf nitrogen concentration (which is assumed to determine maximum carboxylation capacity), and in combination with estimates of the fraction of absorbed radiation it also leads to simple ,big-leaf' analytical solutions for canopy photosynthesis. We tested how forests deviate from this condition in five tree canopies, including four broadleaf stands, and one needle-leaf stand: a mixed-species tropical rain forest, oak (Quercus petraea (Matt.) Liebl), birch (Betula pendula Roth), beech (Fagus sylvatica L.) and Sitka spruce (Picea sitchensis (Bong.) Carr). Each canopy was studied when fully developed (mid-to-late summer for temperate stands). Irradiance (Q, µmol m,2 s,1) was measured for 20 d using quantum sensors placed throughout the vertical canopy profile. Measurements were made to obtain parameters from leaves adjacent to the radiation sensors: maximum carboxylation and electron transfer capacity (Va, Ja, µmol m,2 s,1), day respiration (Rda, µmol m,2 s,1), leaf nitrogen concentration (Nm, mg g,1) and leaf mass per unit area (La, g m,2). Relative to upper-canopy values, Va declined linearly in 1 : 1 proportion with Na. Relative Va also declined linearly with relative Q, but with a significant intercept at zero irradiance (P < 0·01). This intercept was strongly related to La of the lowest leaves in each canopy (P < 0·01, r2 = 0·98, n= 5). For each canopy, daily lnQ was also linearly related with lnVa(P < 0·05), and the intercept was correlated with the value for photosynthetic capacity per unit nitrogen (PUN: Va/Na, µmol g,1 s,1) of the lowest leaves in each canopy (P < 0·05). Va was linearly related with La and Na(P < 0·01), but the slope of the Va : Na relationship varied widely among sites. Hence, whilst there was a unique Va : Na ratio in each stand, acclimation in Va to Q varied predictably with La of the lowest leaves in each canopy. The specific leaf area, Lm(cm2 g,1), of the canopy-bottom foliage was also found to predict carboxylation capacity (expressed on a mass basis; Vm, µmol g,1 s,1) at all sites (P < 0·01). These results invalidate the hypothesis of full acclimation to irradiance, but suggest that La and Lm of the most light-limited leaves in a canopy are widely applicable indicators of the distribution of photosynthetic capacity with height in forests. [source]

An analytical model for the rapid intensification of tropical cyclones

THE QUARTERLY JOURNAL OF THE ROYAL METEOROLOGICAL SOCIETY, Issue 642 2009
Chanh Q. Kieu
Abstract The nonlinearity and complexity of the primitive equations have been key obstacles to our understanding of tropical cyclones (TCs), particularly in relation to the dynamical processes leading to their rapid intensification. In this study, an axisymmetric model, in which all nonlinear terms in the horizontal momentum equations are retained, is used to examine analytically the effects of organized deep convection on TC rapid intensification. By prescribing a vertical profile of the vertical motion with exponential growth in the core region, a class of exact time-dependent solutions for the primary circulations of TCs are obtained. The analytical solutions are shown to capture well many observed dynamical structures in both the core and outer regions and the rapid growth of TCs in terms of maximum winds and central pressure drops. The analytical solutions reveal that (1) the rotational flows in the inner-core region grow double-exponentially, and the central pressure drops occur at rates much faster than the rotational growth; (2) the amplification rates of the primary circulations differ profoundly from those of the secondary circulations; (3) the rotational flows tend to grow from the bottom upwards with the fastest growth occurring at the lowest levels; and (4) the TC growth rates depend critically on the vertical structure of tangential flows, with a faster rate for a lower-level peak rotation. The nonlinear dynamics are shown to play an important role in the rapid growth of TCs. It is demonstrated that the analytical solutions can also be used to construct dynamically consistent vortices for the initialization of TC models. Limitations and possible improvements of the analytical model are also discussed. Copyright © 2009 Royal Meteorological Society [source]

A consistent vertical Bowen ratio profile in the planetary boundary layer

THE QUARTERLY JOURNAL OF THE ROYAL METEOROLOGICAL SOCIETY, Issue 620 2006
M. Hantel
Abstract It has recently been suggested that the integrand b=,,,,, of the subgrid-scale conversion rate between available and kinetic energy has a measurable impact upon the Lorenz energy cycle. Here we discuss a technique to estimate this quantity within the lower part of an atmospheric column by relating b to the subgrid-scale fluxes of sensible and latent heat in form of their sum (the total convective heat flux, c, to be diagnosed from the pertinent energy law) and their ratio (a generalized Bowen ratio, ,, to be specified a priori). We focus on the frequently observed case that c vanishes at or above the top of the boundary layer, which implies that , must be minus unity at the same level (referred to as ,critical pressure'). , at the earth's surface is taken as measured. Observations suggest that the vertical curvature of the , profile is negative in the boundary layer. We specify an analytic vertical profile ,(,) that interpolates these pieces of information; , is a non-dimensional vertical coordinate. The pertinent thermodynamic energy law from which the column profile c(,) is gained (referred to here as convection equation) is driven by the (observed) grid-scale budget; the solution c is over most of the boundary layer quite insensitive to ,. It is only in the immediate vicinity of the critical pressure that c(,) becomes sensitively dependent upon ,(,); it actually turns infinite at this level (a ,pole' of the convection equation). We remove the pole through adjusting the critical pressure by a uniquely determined (and actually quite small) amount. This makes the , profile consistent with the convection equation and with the other convective flux profiles, across the entire boundary layer. The remaining open parameter that cannot be fixed by our method is the curvature of the Bowen ratio profile. This exercise has implications for about a third of all atmospheric columns over the globe and thus may be relevant for the quantification of the global energy cycle. Copyright © 2006 Royal Meteorological Society [source]

Wavelet analysis and the governing dynamics of a large-amplitude mesoscale gravity-wave event along the East Coast of the United States

THE QUARTERLY JOURNAL OF THE ROYAL METEOROLOGICAL SOCIETY, Issue 577 2001
Fuqing Zhang
Abstract Detailed diagnostic analyses are performed upon a mesoscale numerical simulation of a well-observed gravity-wave event that occurred on 4 January 1994 along the East Coast of the United States. The value of using wavelet analysis to investigate the evolving gravity-wave structure and of using potential vorticity (PV) inversion to study the nature of the flow imbalance in the wave generation region is demonstrated. The cross-stream Lagrangian Rossby number, the residual in the nonlinear balance equation, and the unbalanced geopotential-height field obtained from PV inversion are each evaluated for their usefulness in diagnosing the flow imbalance. All of these fields showed clear evidence of strong imbalance associated with a middle-to-upper tropospheric jet streak, and tropopause fold upstream of the large-amplitude gravity wave several hours before the wave became apparent at the surface. Analysis indicates that a train of gravity waves was continuously generated by geostrophic adjustment in the exit region of the unbalanced upper-level jet streak as it approached the inflection axis in the height field immediately downstream of the maximum imbalance associated with the tropopause fold. A split front in the middle troposphere, characterized by the advance of the dry conveyor belt above the warm front, was overtaken by one of these propagating waves. During this merger process, a resonant interaction resulted, which promoted the rapid amplification and scale contraction of both the incipient wave (nonlinear wave development) and the split front (frontogenesis). The gravity wave and front aloft became inseparable following this merger. The situation became even more complex within a few hours as the vertical motion enhanced by this front-wave interaction acted upon a saturated, potentially unstable layer to produce elevated moist convection. An analysis of the temporal changes in the vertical profile of wave energy flux suggests that moist convective downdraughts efficiently transported the wave energy from the midlevels downward beneath the warm-front surface, where the wave became ducted. However, pure ducting was not sufficient for maintaining and amplifying the waves; rather, wave-CISK (Conditional Instability of the Second Kind) was crucial. This complex sequence of nonlinear interactions produced a long-lived, large-amplitude gravity wave that created hazardous winter weather and disrupted society over a broad and highly populated area. Although gravity waves with similar appearance to this large-amplitude wave of depression occasionally have been seen in other strong cyclogenesis cases involving a jet streak ahead of the upper-level trough axis, it is unknown whether other such events share this same sequence of interactions. [source]

Dynamic Systems of Petroleum Accumulation in the Nanpu Depression, Bohai Bay Basin, China

ACTA GEOLOGICA SINICA (ENGLISH EDITION), Issue 5 2010
LIU Guangdi
Abstract: It is significant to distinguish the dynamic systems of petroleum accumulation (DSPA) for the understanding of petroleum accumulation and distribution. According to the formation pressure framework, genetic types of petroleum and characteristics of conduit systems, three dynamic systems of petroleum accumulation were identified in the vertical profile in the Nanpu depression, Bohai Bay basin. The deeper DSPA (including formations Es3 to Es2) is a sealed system with high-overpressure and high-mature self-sourced oil. Most of the crude oil in the system accumulated in the periods of late Oligocene (23.5 Ma) and late Pliocene (2.4 Ma). The middle DSPA (including formations Es1 to Ed1) is an overpressured half-sealed system with mature or lower-mature self-sourced oil. The accumulation of oil in the system also occurred in the late Oligocene (23.5 Ma) and late Pliocene (2.4 Ma). The shallower DSPA (including formations Ed2 to Q) is a hydrostatic system with lower-mature alien-sourced oil from the middle system. Oil within this system accumulated only in the late Pliocene period. The oil in the shallower system migrated vertically along the faults from the formerly accumulated oil in the middle system by lateral migration along the sandbodies, whereas petroleum accumulation in the deeper system was mainly derived from the system itself by lateral migration along the sandbodies and rarely migrated out of the system. In this case, it seems that the deeper system is a more potential exploration prospect in addition to the other two proved favorable systems. [source]

Large-scale distribution and activity patterns of an extremely low-light-adapted population of green sulfur bacteria in the Black Sea

ENVIRONMENTAL MICROBIOLOGY, Issue 5 2010
Evelyn Marschall
Summary The Black Sea chemocline represents the largest extant habitat of anoxygenic phototrophic bacteria and harbours a monospecific population of Chlorobium phylotype BS-1. High-sensitivity measurements of underwater irradiance and sulfide revealed that the optical properties of the overlying water column were similar across the Black Sea basin, whereas the vertical profiles of sulfide varied strongly between sampling sites and caused a dome-shaped three-dimensional distribution of the green sulfur bacteria. In the centres of the western and eastern basins the population of BS-1 reached upward to depths of 80 and 95 m, respectively, but were detected only at 145 m depth close to the shelf. Using highly concentrated chemocline samples from the centres of the western and eastern basins, the cells were found to be capable of anoxygenic photosynthesis under in situ light conditions and exhibited a photosynthesis,irradiance curve similar to low-light-adapted laboratory cultures of Chlorobium BS-1. Application of a highly specific RT-qPCR method which targets the internal transcribed spacer (ITS) region of the rrn operon of BS-1 demonstrated that only cells at the central station are physiologically active in contrast to those at the Black Sea periphery. Based on the detection of ITS-DNA sequences in the flocculent surface layer of deep-sea sediments across the Black Sea, the population of BS-1 has occupied the major part of the basin for the last decade. The continued presence of intact but non-growing BS-1 cells at the periphery of the Black Sea indicates that the cells can survive long-distant transport and exhibit unusually low maintenance energy requirements. According to laboratory measurements, Chlorobium BS-1 has a maintenance energy requirement of ,1.6,4.9·10,15 kJ cell,1 day,1 which is the lowest value determined for any bacterial culture so far. Chlorobium BS-1 thus is particularly well adapted to survival under the extreme low-light conditions of the Black Sea, and can be used as a laboratory model to elucidate general cellular mechanisms of long-term starvation survival. Because of its adaptation to extreme low-light marine environments, Chlorobium BS-1 also represents a suitable indicator for palaeoceanography studies of deep photic zone anoxia in ancient oceans. [source]

Experimental and modeling investigation of metal release from metal-spiked sediments

ENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 12 2005
Richard F. Carbonaro
Abstract In sediments that contain iron monosulfide, cadmium, nickel, lead, zinc, and silver(I) form insoluble metal sulfides that lower the metal ion activity in the sediment,pore water system, thereby reducing toxicity. However, metal sulfides are susceptible to oxidation by molecular oxygen resulting in metal solubilization. To better understand the sources and sinks of metal sulfides in sediments, iron monsulfide,rich freshwater sediments were spiked with cadmium, nickel, lead, zinc, or silver(I) and placed into cylindrical cores with an overlying layer of oxygen-saturated water. Measurements of the dissolved metal concentration in the overlying water were made as a function of time and the vertical profiles of acid-volatile sulfide (AVS) and simultaneously extracted metal (SEM) were measured after 150 d. A one-dimensional reactive and transport model has been employed to help elucidate processes controlling the fate of metals in sediments. The model incorporates metal-sulfide formation, metal-sulfide oxidation, and metal partitioning onto sediment organic carbon and iron oxyhydroxide to simulate the vertical transport of metals throughout the sediment core. [source]

Earth's magnetic field: ocean current contributions to vertical profiles in deep oceans

GEOPHYSICAL JOURNAL INTERNATIONAL, Issue 1 2001
F. E. M. (Ted) Lilley
SUMMARY The Earth's main magnetic field, arising in the core, should, in the ocean, have a well-defined uniform gradient with depth. Superimposed upon this uniform gradient may be magnetic signals due to a variety of sources. These include crustal magnetization, the transient fluctuations arising external to the Earth and causing secondary induced fields within it; and, the focus of the present paper, magnetic signals arising from the motional induction of seawater moving in the steady main magnetic field of Earth. There are circumstances where theory predicts such motionally-induced magnetic fields to be of order 102 nT, and to vary with depth in a way which is directly related to the velocity profile. Exploratory soundings of the magnetic field with depth have been made in the oceans around Australia, both to test these predictions, and to investigate the practicability of measuring such profiles. The magnetic field parameter observed has been that of the ,total field', which should sense the component of the ocean velocity which lies in the magnetic meridian. The magnetometer has been lowered by cable from a ship and also operated free-fall to the seafloor (and return). The observations appear both to confirm the theoretical gradient of the main field where there is no ocean current and, where ocean currents exist, to give evidence of their profiles resolved in the direction of magnetic north. In particular, observations taken in an eddy of the East Australian Current show the correct contrast in sign for north and south flowing streams. [source]

Inferring nocturnal surface fluxes from vertical profiles of scalars in an Amazon pasture

GLOBAL CHANGE BIOLOGY, Issue 5 2004
Otávio C. Acevedo
Abstract Ecosystem carbon budgets depend on there being good representative surface flux observations for all land use types during the entire diurnal cycle. In calm conditions that often occur at night, especially in areas of small roughness (such as pastures), ecosystem respiration rate is poorly measured using the eddy covariance (EC) technique. Nocturnal vertical profiles of temperature, humidity and winds were observed using tethered balloon soundings in a pasture in the eastern Amazon during two campaigns in 2001. The site is characterized by very weak winds at night, so that there is insufficient turbulence for the EC technique to determine fluxes accurately. To compensate, the time evolution of the profiles is used to determine surface fluxes at early morning and these are compared with those observed by EC at a nearby micrometeorological tower. The nocturnal boundary layer thickness h is determined as the height to which the surface fluxes must converge so that energy budget closure is achieved. The estimated values range from 30 m, around 22:00 hours LST, to more than 100 m just before dawn. These are in good agreement with the observed thickness of a frequently observed fog layer during the middle of the night. During the early portion of the night, when the accumulation layer is shallow, there is appreciable decrease of dCO2/dt with height. On calm nights, CO2 accumulation rate is larger near the surface than at higher levels. On windier nights, this accumulation rate is vertically uniform. Hence, extrapolation of tower profiles for estimating fluxes must be done carefully. Although uncertainties remain large, an alternate approach to the EC method is described for measuring nighttime surface CO2 fluxes under stable atmospheric conditions. [source]

A New Multilevel Ground Water Monitoring System Using Multichannel Tubing

GROUND WATER MONITORING & REMEDIATION, Issue 4 2002
Murray D. Einarson
A new multilevel ground water monitoring system has been developed that uses custom-extruded flexible 1.6-inch (4.1 cm) outside-diameter (O.D.) multichannel HOPE tubing (referred to as Continuous Multichannel Tubing or CMT) to monitor as many as seven discrete zones within a single borehole in either unconsolidated sediments or bedrock. Prior to inserting the tubing in the borehole, ports are created that allow ground water to enter six outer pie-shaped channels (nominal diameter = 0.5 inch [1.3 cm]) and a central hexagonal center channel (nominal diameter = 0.4 inch [1 cm]) at different depths, facilitating the measurement of depth-discrete piezometric heads and the collection of depth-discrete ground water samples. Sand packs and annular seals between the various monitored zones can be installed using conventional tremie methods. Alternatively, bentonite packers and prepacked sand packs have been developed that are attached to the tubing at the ground surface, facilitating precise positioning of annular seals and sand packs. Inflatable rubber packers for permanent or temporary installations in bedrock aquifers are currently undergoing site trials. Hydraulic heads are measured with conventional water-level meters or electronic pressure transducers to generate vertical profiles of hydraulic head. Ground water samples are collected using peristaltic pumps, small-diameter bailers, inertial lift pumps, or small-diameter canister samplers. For monitoring hydrophobic organic compounds, the CMT tubing is susceptible to both positive and negative biases caused by sorption, desorption, and diffusion. These biases can be minimized by: (1) purging the channels prior to sampling, (2) collecting samples from separate 0.25-inch (0.64 cm) O.D. Teflon sampling tubing inserted to the bottom of each sampling channel, or (3) collecting the samples downhole using sampling devices positioned next to the intake ports. More than 1000 CMT multilevel wells have been installed in North America and Europe to depths up to 260 feet (79 m) below ground surface. These wells have been installed in boreholes created in unconsolidated sediments and bedrock using a wide range of drilling equipment, including sonic, air rotary, diamond-bit coring, hollow-stem auger, and direct push. This paper presents a discussion of three field trials of the system, demonstrating its versatility and illustrating the type of depth-discrete data that can be collected with the system. [source]

Laboratory experimental check of a conceptual model for infiltration under complex rainfall patterns

The impact of vertical resolution on regional model simulation of the west African summer monsoon

INTERNATIONAL JOURNAL OF CLIMATOLOGY, Issue 10 2008
Leonard M. Druyan
Abstract The RM3 regional climate model is used to simulate the west African summer monsoon for six June,September seasons using NCEP reanalysis data for lateral boundary forcing. The study compares the performance of the previously published 16-level version with a newly tested 28-level version, both running on a horizontal grid with 0.5° spacing, in order to determine what improvements in simulations are achieved by increased vertical resolution. Comparisons between the performances include diagnostics of seasonal mean precipitation rates and circulation, vertical profiles of cumulus heating rates, frequencies of shallow and deep convection and diagnostics related to transient African easterly waves (AEWs). The characteristics of a composite AEW simulated at both vertical resolutions are presented. Results show that the most significant impact of increasing the vertical resolution is stronger circulation, stronger vertical wind shear and higher amplitude AEWs. The simulations with higher vertical resolution also achieve higher peaks of cumulus latent heating rates. Spatial,temporal correlations between simulated daily 700 mb meridional winds versus corresponding NCEP reanalysis data and simulated daily precipitation versus estimates from the Tropical Rainfall Measurement Mission (TRMM) archive were equally high at both vertical resolutions. Copyright © 2007 Royal Meteorological Society [source]

Modeling heterogeneous downward dense gas-particle flows

AICHE JOURNAL, Issue 5 2010
Regis Andreux
Abstract A novel approach is proposed to model heterogeneous downward dense gas-particle flows. The homogeneous behavior of the flow is described by the mass and momentum transport equations of the gas and particulate phases solved using a mono-dimension finite volume method on staggered grids. The heterogeneous features of the flow are predicted simultaneously using the bubble-emulsion formalism. The gas compressibility is taken into consideration. The model is supplemented with a new correlation to account for the wall-particle frictional effects. The predictions are compared with the vertical profiles of pressure and the amount of gas that flows up and down two standpipes and a cyclone dipleg of an industrial fluid catalytic cracking unit and of a cold small-scale circulating fluidized bed. The trends are well predicted. The model gives further information and is thus an innovative starting point for downward dense gas-particle flow hydrodynamics investigation. © 2009 American Institute of Chemical Engineers AIChE J, 2010 [source]

The development of a new dust uplift scheme in the Met Office Unified ModelÔ

METEOROLOGICAL APPLICATIONS, Issue 4 2009
D. Ackerley
Abstract Aeolian mineral dust aerosol is an important consideration in the Earth's radiation budget as well as a source of nutrients to oceanic and land biota. The modelling of aeolian mineral dust has been improving consistently despite the relatively sparse observations to constrain them. This study documents the development of a new dust emissions scheme in the Met Office Unified ModelÔ (MetUM) based on the Dust Entrainment and Deposition (DEAD) module. Four separate case studies are used to test and constrain the model output. Initial testing was undertaken on a large dust event over North Africa in March 2006 with the model constrained using AERONET data. The second case study involved testing the capability of the model to represent dust events in the Middle East without being re-tuned from the March 2006 case in the Sahara. While the model is unable to capture some of the daytime variation in AERONET AOD there is good agreement between the model and observed dust events. In the final two case studies new observations from in situ aircraft data during the Dust Outflow and Deposition to the Ocean (DODO) campaigns in February and August 2006 were used. These recent observations provided further data on dust size distributions and vertical profiles to constrain the model. The modelled DODO cases were also compared to AERONET data to make sure the radiative properties of the dust were comparable to observations. Copyright © 2009 Royal Meteorological Society and Crown Copyright [source]

Assimilation of radar reflectivity into the LM COSMO model with a high horizontal resolution

METEOROLOGICAL APPLICATIONS, Issue 4 2006
Z. Sokol
Abstract An assimilation of radar reflectivity into a numerical weather prediction (NWP) model with a horizontal resolution of 2.8 km is presented and applied to three severe convective events. The suggested assimilation method takes into account differences between the model and radar-derived precipitation in modifying vertical profiles of water vapour mixing ratio in each model time step by the nudging approach. Version 3.9 of the LM COSMO (Local Model COSMO) ,NWP model used in this study includes the explicit formulation of the cloud and rain processes involved. Two variants of the assimilation technique are designed and outputs of their implementation are compared. The first variant makes use of the ground data only, while the second utilises vertical profiles of precipitation water. Both variants provide an improvement of precipitation forecast in comparison with outputs of the control run without assimilation procedures applied. When the assimilated radar data indicate initial precipitation near an expected storm, the NWP model is capable of forecasting basic features of the storm development two to three hours ahead. Three case studies are presented. In one, the assimilation method that takes into account the vertical structure of the precipitation water yields better results than the others which utilise ground data only. However, for the remaining two case studies both types of the assimilation method produce comparable results. Copyright © 2006 John Wiley & Sons, Ltd. [source]

Modeling and Simulation of Notional Future Radar in Non-Standard Propagation Environments Facilitated by Mesoscale Numerical Weather Prediction Modeling

Spatial separation of litter decomposition and mycorrhizal nitrogen uptake in a boreal forest

NEW PHYTOLOGIST, Issue 3 2007
Björn D. Lindahl
Summary ,,Our understanding of how saprotrophic and mycorrhizal fungi interact to re-circulate carbon and nutrients from plant litter and soil organic matter is limited by poor understanding of their spatiotemporal dynamics. ,,In order to investigate how different functional groups of fungi contribute to carbon and nitrogen cycling at different stages of decomposition, we studied changes in fungal community composition along vertical profiles through a Pinus sylvestris forest soil. We combined molecular identification methods with 14C dating of the organic matter, analyses of carbon:nitrogen (C:N) ratios and 15N natural abundance measurements. ,,Saprotrophic fungi were primarily confined to relatively recently (< 4 yr) shed litter components on the surface of the forest floor, where organic carbon was mineralized while nitrogen was retained. Mycorrhizal fungi dominated in the underlying, more decomposed litter and humus, where they apparently mobilized N and made it available to their host plants. ,,Our observations show that the degrading and nutrient-mobilizing components of the fungal community are spatially separated. This has important implications for biogeochemical studies of boreal forest ecosystems. [source]