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Versatile Strategy (versatile + strategy)

Distribution by Scientific Domains

Polymers and Materials Science	50%

Selected Abstracts

A Flexible and Versatile Strategy for the Covalent Immobilization of Chiral Catalysts Based on Pyridinebis(oxazoline) Ligands.

CHEMINFORM, Issue 49 2005
Alfonso Cornejo
Abstract For Abstract see ChemInform Abstract in Full Text. [source]

Dual Stimuli-Responsive Supramolecular Polypeptide-Based Hydrogel and Reverse Micellar Hydrogel Mediated by Host,Guest Chemistry

ADVANCED FUNCTIONAL MATERIALS, Issue 4 2010
Yi Chen
Abstract Versatile strategies are currently being discovered for the fabrication of synthetic polypeptide-based hybrid hydrogels, which have potential applications in polymer therapeutics and regenerative medicine. Herein, a new concept,the reverse micellar hydrogel,is introduced, and a versatile strategy is provided for fabricating supramolecular polypeptide-based normal micellar hydrogel and reverse micellar hydrogels from the same polypeptide-based copolymer via the cooperation of host,guest chemistry and hydrogen-bonding interactions. The supramolecular hydrogels are thoroughly characterized, and a mechanism for their self-assembly is proposed. These hydrogels can respond to dual stimuli,temperature and pH,and their mechanical and controlled drug-release properties can be tuned by the copolymer topology and the polypeptide composition. The reverse micellar hydrogel can load 10% of the anticancer drug doxorubicin hydrochloride (DOX) and sustain DOX release for 45 days, indicating that it could be useful as an injectable drug delivery system. [source]

Capturing Complex Protein Gradients on Biomimetic Hydrogels for Cell-Based Assays

ADVANCED FUNCTIONAL MATERIALS, Issue 21 2009
Steffen Cosson
Abstract A versatile strategy to rapidly immobilize complex gradients of virtually any desired protein on soft poly(ethylene glycol) (PEG) hydrogel surfaces that are reminiscent of natural extracellular matrices (ECM) is reported. A microfluidic chip is used to generate steady-state gradients of biotinylated or Fc-tagged fusion proteins that are captured and bound to the surface in less than 5,min by NeutrAvidin or ProteinA, displayed on the surface. The selectivity and orthogonality of the binding schemes enables the formation of parallel and orthogonal overlapping gradients of multiple proteins, which is not possible on conventional cell culture substrates. After patterning, the hydrogels are released from the microfluidic chip and used for cell culture. This novel platform is validated by conducting single-cell migration experiments using time-lapse microscopy. The orientation of cell migration, as well as the migration rate of primary human fibroblasts, depends on the concentration of an immobilized fibronectin fragment. This technique can be readily applied to other proteins to address a wealth of biological questions with different cell types. [source]

A versatile approach for the syntheses of poly(ester amide)s with pendant functional groups

JOURNAL OF POLYMER SCIENCE (IN TWO SECTIONS), Issue 15 2009
Katelyn M. Atkins
Abstract Poly(ester amide)s (PEAs) are emerging as promising materials for a wide range of biomedical applications due to their potential for both hydrolytic and enzymatic degradation, as well as the ease with which their properties can be tuned by the choice of monomers. The incorporation of pendant functional handles along the PEA backbone has the potential to further expand their applications by allowing the charge and hydrophilicity of the polymers to be altered, and facilitating the conjugation of active molecules such as drugs, targeting groups, and cell signaling molecules. Described here is a simple and versatile strategy based on orthogonal protecting groups, by which L -lysine and L- aspartic acid can be incorporated into several families of PEAs based on monomers including the diacids succinic and terephthalic acid, the diols 1,4-butanediol and 1,8-octanediol, and the amino acids L- alanine and L- phenylalanine. All polymers were thoroughly characterized by nuclear magnetic resonance spectroscopy, infrared spectroscopy, size exclusion chromatography, thermogravimetric analysis, and differential scanning calorimetry. It was demonstrated that the side chain protecting groups could be readily removed, allowing the pendant amines or carboxylic acids to be functionalized. In particular, the carboxylic acid groups on a polymer containing L- aspartic acid units were converted to N -hydroxysuccinimidyl esters, providing a useful template for further derivatization. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 3757,3772, 2009 [source]

Induction and analysis of cell adhesion and differentiation on inkjet micropatterned substrates

PHYSICA STATUS SOLIDI (C) - CURRENT TOPICS IN SOLID STATE PHYSICS, Issue 6 2007
A. Blau
Abstract In the context of interfacing biological entities to technical substrates, proof of principle results on patterning cell culturing substrates by inkjet deposition of the cell adhesion mediator polyethyleneimine are presented. Pattern fidelity, cell adhesion, and cell differentiation of chick neurons and glia cells on such interfaces were evaluated microscopically. The exemplary results demonstrate that piezo inkjet printing of biologically active compounds has the potential of becoming an easy to handle and versatile strategy for applying a wide variety of substances in any combination and concentration with cellular to subcellular resolution onto a wide selection of substrates. (© 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source]

A versatile strategy to define the phosphorylation preferences of plant protein kinases and screen for putative substrates

THE PLANT JOURNAL, Issue 1 2008
Florina Vlad
Summary Most signaling networks are regulated by reversible protein phosphorylation. The specificity of this regulation depends in part on the capacity of protein kinases to recognize and efficiently phosphorylate particular sequence motifs in their substrates. Sequenced plant genomes potentially encode over than 1000 protein kinases, representing 4% of the proteins, twice the proportion found in humans. This plethora of plant kinases requires the development of high-throughput strategies to identify their substrates. In this study, we have implemented a semi-degenerate peptide array screen to define the phosphorylation preferences of four kinases from Arabidopsis thaliana that are representative of the plant calcium-dependent protein kinase and Snf1-related kinase superfamily. We converted these quantitative data into position-specific scoring matrices to identify putative substrates of these kinases in silico in protein sequence databases. Our data show that these kinases display related but nevertheless distinct phosphorylation motif preferences, suggesting that they might share common targets but are likely to have specific substrates. Our analysis also reveals that a conserved motif found in the stress-related dehydrin protein family may be targeted by the SnRK2-10 kinase. Our results indicate that semi-degenerate peptide array screening is a versatile strategy that can be used on numerous plant kinases to facilitate identification of their substrates, and therefore represents a valuable tool to decipher phosphorylation-regulated signaling networks in plants. [source]