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Variety Of Processes (variety + of_process)
Selected AbstractsSemaphorin and neuropilin expression during early morphogenesis of Xenopus laevisDEVELOPMENTAL DYNAMICS, Issue 12 2008Ulrich Koestner Abstract Semaphorins are major regulators of morphogenesis and are involved in a variety of processes ranging from the guidance of cell migration to the development of cancer. Since semaphorins were first characterized as repulsive neuronal guidance cues, their expression has been best documented in the nervous system. However, broader studies are lacking. Here, we describe the expression of 13 members of the semaphorin family and two neuropilin receptors during early Xenopus laevis development. No particular expression pattern defines any of the semaphorin classes, but many are dynamically expressed in distinct areas undergoing morphogenetic cell movements like the developing mesoderm and the migrating neural crest. Furthermore, the complementary expression patterns of Sema3A/Nrp1 and Sema3F/Nrp2 are maintained across hundreds of millions of years, possibly indicating a conserved role in the guidance of migrating neural crest cells. Developmental Dynamics 237:3853,3863, 2008. © 2008 Wiley-Liss, Inc. [source] Electrochemistry at High Pressures: A ReviewELECTROANALYSIS, Issue 10 2004Debora Giovanelli Abstract High pressure electrochemical studies are potentially dangerous and less immediately implemented than conventional investigations. Technical obstacles related to properties of the working electrode material, preparation of its surface, availability of suitable reference electrodes, and the need for specially designed high pressure equipment and cells may account for the relative lack of experimental data on electrochemistry at high pressures. However, despite the stringent requirements for system and equipment stability, significant developments have been made in recent years and the combination of electrochemical methods with high hydrostatic pressure has provided useful insights into the thermodynamics, kinetics, and other physico-chemical characteristics of a wide range of redox reactions. In addition to fundamental information, high pressure electrochemistry has also lead to a better understanding of a variety of processes under non-classical conditions with potential applications in today's industrial environment from extraction and electrosynthesis in supercritical fluids to measurement of the pH at the bottom of the ocean. The purpose of this article is to detail the experimental pressurizing apparatus for electroanalytical measurements at high pressures and to review the relevant literature on the effect of pressure on electrode processes and on the properties of aqueous electrolyte solutions. [source] Novel domains of the prokaryotic two-component signal transduction systemsFEMS MICROBIOLOGY LETTERS, Issue 1 2001Michael Y. Galperin Abstract The archetypal two-component signal transduction systems include a sensor histidine kinase and a response regulator, which consists of a receiver CheY-like domain and a DNA-binding domain. Sequence analysis of the sensor kinases and response regulators encoded in complete bacterial and archaeal genomes revealed complex domain architectures for many of them and allowed the identification of several novel conserved domains, such as PAS, GAF, HAMP, GGDEF, EAL, and HD-GYP. All of these domains are widely represented in bacteria, including 19 copies of the GGDEF domain and 17 copies of the EAL domain encoded in the Escherichia coli genome. In contrast, these novel signaling domains are much less abundant in bacterial parasites and in archaea, with none at all found in some archaeal species. This skewed phyletic distribution suggests that the newly discovered complexity of signal transduction systems emerged early in the evolution of bacteria, with subsequent massive loss in parasites and some horizontal dissemination among archaea. Only a few proteins containing these domains have been studied experimentally, and their exact biochemical functions remain obscure; they may include transformations of novel signal molecules, such as the recently identified cyclic diguanylate. Recent experimental data provide the first direct evidence of the participation of these domains in signal transduction pathways, including regulation of virulence genes and extracellular enzyme production in the human pathogens Bordetella pertussis and Borrelia burgdorferi and the plant pathogen Xanthomonas campestris. Gene-neighborhood analysis of these new domains suggests their participation in a variety of processes, from mercury and phage resistance to maintenance of virulence plasmids. It appears that the real picture of the complexity of phosphorelay signal transduction in prokaryotes is only beginning to unfold. [source] Histone modifications and chromatin dynamics: a focus on filamentous fungiFEMS MICROBIOLOGY REVIEWS, Issue 3 2008Gerald Brosch Abstract The readout of the genetic information of eukaryotic organisms is significantly regulated by modifications of DNA and chromatin proteins. Chromatin alterations induce genome-wide and local changes in gene expression and affect a variety of processes in response to internal and external signals during growth, differentiation, development, in metabolic processes, diseases, and abiotic and biotic stresses. This review aims at summarizing the roles of histone H1 and the acetylation and methylation of histones in filamentous fungi and links this knowledge to the huge body of data from other systems. Filamentous fungi show a wide range of morphologies and have developed a complex network of genes that enables them to use a great variety of substrates. This fact, together with the possibility of simple and quick genetic manipulation, highlights these organisms as model systems for the investigation of gene regulation. However, little is still known about regulation at the chromatin level in filamentous fungi. Understanding the role of chromatin in transcriptional regulation would be of utmost importance with respect to the impact of filamentous fungi in human diseases and agriculture. The synthesis of compounds (antibiotics, immunosuppressants, toxins, and compounds with adverse effects) is also likely to be regulated at the chromatin level. [source] ,Have You Got a Boyfriend or are You Single?': On the Importance of Being ,Straight' in Organizational ResearchGENDER, WORK & ORGANISATION, Issue 3 2006Attila Bruni The article focuses on heterosexuality as a covert feature of organization studies as well as of organizational research. In fact, while organization studies have discussed the gendered and the gendering aspects of organizational practices and organizational theory, the implication of heterosexuality has yet to receive intensive analysis in these fields. And while the mutual and reflexive constitution of the observer and the observed has been the topic of a considerable amount of research, the dimension of (heterosexual) desire in this process of mutual constitution is still largely unexplored. Referring to three different episodes that occurred while the author was doing organizational ethnography, the article suggests that a heterosexual model of desire is called into action both in organizational and research activities and that focusing on it can be an occasion to question not only the gender (and heterosexual) biases of organizational practices but also the way in which gender and sexuality are mobilized while doing research. In particular, on the basis of the concept of cathexis, the article shows how heterosexuality is learnt and enacted as a situated practice and through a variety of processes: performing power, negotiating and displaying that one belongs to an organizational culture, obscuring the hetero-normativity of professional identities and neglecting the emotional engagement that characterizes research activities and that exposes the researcher to an otherwise vulnerable position. [source] Turf exfoliation in the high Drakensberg, Southern AfricaGEOGRAFISKA ANNALER SERIES A: PHYSICAL GEOGRAPHY, Issue 1 2002Stefan W. Grab Limited research attention has focussed on turf exfoliation as a denudation process in mountain environments. This paper examines some characteristics of turf exfoliation forms identified within particular valley zones in the Drakensberg alpine belt. Morphological and sedimentological data are presented for turf exfoliated sites investigated in the Mashai Valley of eastern Lesotho. It is found that a variety of processes, including needle ice action, biological activity, fluvial processes and deflation, operating synergistically, are responsible for contemporary turf exfoliation in the high Drakensberg. It is apparent that the strong seasonality from mild, wet summers to cold, dry winters has helped induce the annual cycle of dominating processes. [source] Inversion of earthquake focal mechanisms to obtain the seismotectonic stress IV,a new method free of choice among nodal planesGEOPHYSICAL JOURNAL INTERNATIONAL, Issue 3 2002Jacques Angelier Summary A new method is presented, to obtain the stress state that best accounts for a set of double couple focal mechanisms of earthquakes. This method is based on the slip shear stress component (SSSC) criterion. The sum of the SSSC values is maximized as a function of four unknowns that describe the reduced stress tensor, including the orientations of the principal stress axes and the ratio between the principal stress differences. This new method combines two advantages. First, no choice between the nodal planes of each focal mechanism is needed, because of the intrinsic properties of the SSSC. Secondly, the runtime is negligible regardless of the size of the data set, because the inverse problem is solved by analytical means so that the numerical aspects are reduced to a minimum. For these reasons, the SSSC-based inversion is easily included in a variety of processes for separating or refining the data. A typical set of focal mechanisms of earthquakes in Taiwan is processed to illustrate the application and potential of the new method. [source] Rafts in oligodendrocytes: Evidence and structure,function relationshipGLIA, Issue 6 2006Ellen Gielen Abstract The plasma membrane of eukaryotic cells exhibits lateral inhomogeneities, mainly containing cholesterol and sphingomyelin, which provide liquid-ordered microdomains (lipid "rafts") that segregate membrane components. Rafts are thought to modulate the biological functions of molecules that become associated with them, and as such, they appear to be involved in a variety of processes, including signal transduction, membrane sorting, cell adhesion and pathogen entry. Although still a matter of ongoing debate, evidence in favor of the presence of these microdomains is gradually accumulating but a consensus on issues like their size, lifetime, composition, and biological significance has yet to be reached. Here, we provide an overview of the evidence supporting the presence of rafts in oligodendrocytes, the myelin-producing cells of the central nervous system, and discuss their functional significance. The myelin membrane differs fundamentally from the plasma membrane, both in lipid and protein composition. Moreover, since myelin membranes are unusually enriched in glycosphingolipids, questions concerning the biogenesis and functional relevance of microdomains thus appear of special interest in oligodendrocytes. The current picture of rafts in oligodendrocytes is mainly based on detergent methods. The robustness of such data is discussed and alternative methods that may provide complementary data are indicated. © 2006 Wiley-Liss, Inc. [source] Accelerating executive transitions at The Home DepotGLOBAL BUSINESS AND ORGANIZATIONAL EXCELLENCE, Issue 5 2008Leslie W. Joyce The Home Depot accelerates "speed to competency" for new executives through a variety of processes and tools tailored to different types of transitions. The approaches focus on the early days of the transition, engage key stakeholders in the process, and emphasize organizational realities, including what it really takes to make it at The Home Depot. The author discusses research into the factors common to successful transitions in the company; the company's acceleration framework; two different approaches, for role-based transitions in the field and context-based transitions at headquarters; and other tools for supporting executives new to the company and/or their roles. © 2008 Wiley Periodicals, Inc. [source] Selecting thioredoxins for disulphide proteomics: Target proteomes of three thioredoxins from the cyanobacterium Synechocystis sp.PROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue S1 2006PCC 680 Abstract Searching for enzymes and other proteins which can be redox-regulated by dithiol/disulphide exchange is a rapidly expanding area of functional proteomics. Recently, several experimental approaches using thioredoxins have been developed for this purpose. Thioredoxins comprise a large family of redox-active enzymes capable of reducing protein disulphides to cysteines and of participating in a variety of processes, such as enzyme modulation, donation of reducing equivalents and signal transduction. In this study we screened the target proteomes of three different thioredoxins from the unicellular cyanobacterium Synechocystis sp. PCC 6803, using site-directed active-site cysteine-to-serine mutants of its m -, x - and y -type thioredoxins. The properties of a thioredoxin that determine the outcome of such analyses were found to be target-binding capacity, solubility and the presence of non-active-site cysteines. Thus, we explored how the choice of thioredoxin affects the target proteomes and we conclude that the m -type thioredoxin, TrxA, is by far the most useful for screening of disulphide proteomes. Furthermore, we improved the resolution of target proteins on non-reducing/reducing 2-DE, leading to the identification of 14 new potentially redox-regulated proteins in this organism. The presence of glycogen phosphorylase among the newly identified targets suggests that glycogen breakdown is redox-regulated in addition to glycogen synthesis. [source] The way of our errors: Theme and variationsPSYCHOPHYSIOLOGY, Issue 1 2010Robert F. Simons Abstract Negative feedback, either internal or external, is a fundamental guide to human learning and performance. The neural system that underlies the monitoring of performance and the adjustment of behavior has been subject to multiple neuroimaging investigations that uniformly implicate the anterior cingulate cortex and other prefrontal structures as crucial to these executive functions. The present article describes a series of experiments that employed event-related potentials to study a variety of processes associated with internal or external feedback. Three medial-frontal negativities (error-related negativity, correct-response negativity, feedback-related negativity) are highlighted, each of which plays an important role in the monitoring and dynamic adjustment of behavior. Extensions of basic research on these ERPs to questions relevant to clinical-science are also provided. [source] Interfacing mind and brain: A neurocognitive model of recognition memoryPSYCHOPHYSIOLOGY, Issue 5 2000Axel Mecklinger A variety of processes contribute to successful recognition memory, some of which can be associated with spatiotemporally distinct event-related potential old/new effects. An early frontal and a subsequent parietal old/new effect are correlated with the familiarity and recollection subcomponents of recognition memory, respectively, whereas a late, postretrieval old/new effect seems to reflect an ensemble of evaluation processes that are set by the task context in which retrieval occurs. Both the early frontal and the parietal old/new effects are differentially modulated by the informational content (e.g., object forms and spatial locations) of recognition and seem to rely on brain systems damaged in amnesia. The late frontal effect appears to reflect prefrontal cortex activation. A neurophysiologically based model of recognition memory retrieval is presented and it is shown that coupling recognition memory subprocesses with distinct old/new effects allow examination of the time course of the processes that contribute to correct and to illusory memories. In conjunction with event-related functional magnetic resonance imaging activation patterns the brain systems recruited by various aspects of episodic memory retrieval can be identified. [source] Photorefractive effect of ferroelectric liquid crystalsTHE CHEMICAL RECORD, Issue 1 2006Takeo Sasaki Abstract This paper reviews our recent work on the photorefractive effect of ferroelectric liquid crystals (FLCs). The photorefractive effect is defined as the optical modulation of the refractive index of a medium as a result of a variety of processes. The interference of two laser beams in a photorefractive material establishes a refractive index grating. This phenomenon enables the creation of different types of photonic applications. FLCs exhibit fast electric field response, and the orientation of the molecular axis of FLCs changes its direction according to the change in direction of the spontaneous polarization (Ps). When two laser beams interfere in a photoconductive FLC, an orientational grating is formed. The mechanism of the formation of the grating is based on the response of the Ps to the photoinduced internal electric field. The time of formation of the refractive index grating is significantly shorter in FLC materials. © 2006 The Japan Chemical Journal Forum and Wiley Periodicals, Inc. Chem Rec 6: 43,51; 2006: Published online in Wiley InterScience (www.interscience.wiley.com) DOI 10.1002/tcr.20071 [source] Analysis of B function in legumes: PISTILLATA proteins do not require the PI motif for floral organ development in Medicago truncatulaTHE PLANT JOURNAL, Issue 1 2009Reyes Benlloch Summary The B-class gene PISTILLATA (PI) codes for a MADS-box transcription factor required for floral organ identity in angiosperms. Unlike Arabidopsis, it has been suggested that legume PI genes contribute to a variety of processes, such as the development of floral organs, floral common petal,stamen primordia, complex leaves and N-fixing root nodules. Another interesting feature of legume PI homologues is that some of them lack the highly conserved C-terminal PI motif suggested to be crucial for function. Therefore, legume PI genes are useful for addressing controversial questions on the evolution of B-class gene function, including how they may have diverged in both function and structure to affect different developmental processes. However, functional analysis of legume PI genes has been hampered because no mutation in any B-class gene has been identified in legumes. Here we fill this gap by studying the PI function in the model legume species Medicago truncatula using mutant and RNAi approaches. Like other legume species, M. truncatula has two PI homologues. The expression of the two genes, MtPI and MtNGL9, has strongly diverged, suggesting differences in function. Our analyses show that these genes are required for petal and stamen identity, where MtPI appears to play a predominant role. However, they appear not to be required for development of the nodule, the common primordia or the complex leaf. Moreover, both M. truncatula PI homologues lack the PI motif, which indicates that the C-terminal motif is not essential for PI activity. [source] | ||||||||||||||||