Home About us Contact
|
Home About us Contact | ||
|
|
||
Vulgaris L. (vulgari + l)
Kinds of Vulgaris L. Selected AbstractsSearching and oviposition behavior of a mymarid egg parasitoid, Anagrus nigriventris, on five host plant species of its leafhopper host, Circulifer tenellusENTOMOLOGIA EXPERIMENTALIS ET APPLICATA, Issue 1 2000A.K. Al-Wahaibi Abstract Searching and oviposition behavior and parasitization ability of Anagrus nigriventris Girault (Hymenoptera: Mymaridae), an egg parasitoid of beet leafhopper, Circulifer tenellus (Baker) (Homoptera: Cicadellidae), were examined on five host plant species of beet leafhopper: sugar beet (Beta vulgaris L.), red stem filaree (Erodium cicutarium[L.]), peppergrass (Lepidium nitidum Nuttall), desert plantain (Plantago ovata Forsskal), and London rocket (Sisymbrium irio L.). Beet leafhopper embeds its eggs in the tissues of these plant species. For each plant species, A. nigriventris behavior was examined on plants with and without beet leafhopper eggs. Experimental design was a 5 (plant species) by 2 (host eggs present/absent) factorial. Additionally within each treatment, parasitoid behavior was observed over a 22-h period at five different observation periods: t=0, 3, 6, 9, and 22 h where t=0 h represents initial exposure of the insect with the plant. The behavioral events observed were: ,fast walking' (general searching), ,slow walking' (intensive searching), ovipositor probing, grooming, feeding, and resting. Significant differences (,=0.05) among plant species in time spent on the plant, percentage of host eggs parasitized, and behavioral variables associated with intensive searching and oviposition all indicated that the plant species fell into two groups: ,preferred' plants (sugar beet, London rocket, and peppergrass), and ,unpreferred' plants (filaree and plantago). These variables also indicated that the parasitoids spent more time on, searched more, probed more, and oviposited more in plants with host eggs than plants without host eggs. Consistent effects of time (over the observation periods from t=0 to t=22 h) generally were detected only in the preferred plant species that had host eggs present. In these cases, intensive searching and probing decreased as time advanced, while variables related to general searching (,fast walking') and abandoning host egg patches (leaving the plant) tended to increase over time. [source] Molecular weight and humification index as predictors of adsorption for plant- and manure-derived dissolved organic matter to goethiteEUROPEAN JOURNAL OF SOIL SCIENCE, Issue 1 2007T. Ohno Summary Sorptive retention of organic matter is important in maintaining the fertility and quality of soils in agricultural ecosystems. However, few sorption studies have been conducted that use dissolved organic matter (DOM) characteristic of agricultural amendments. We investigated the sorption to goethite (,-FeOOH) of DOM extracted from: (i) above-ground biomass of wheat straw (Triticum aestivum L.), maize residue (Zea mays L.), soybean residue (Glycine max (L.) Merr.), and hairy vetch residue (Vivia billosa L.); (ii) below-ground biomass from maize, soybean, canola (Brassica napus L.), and green bean (Phaseolus vulgaris L.); and (iii) beef, dairy, poultry, and pig animal manures. The apparent molecular weight (MWAP) of the DOM was measured by high performance-size exclusion chromatography and ranged from 312 to 1074 g mol,1. The carboxyl-group content of the DOM measured by potentiometric titration ranged from 4.84 to 21.38 mmol(,) g,1 carbon. The humification index (HIX) determined by fluorescence spectrometry varied from 1.15 to 4.33. Sorption was directly related to both MWAP and HIX values of the DOM. Molecular weight analysis of the solution prior to and after sorption indicated that the DOM molecules > 1800 g mol,1 were preferentially sorbed, resulting in fractionation of the DOM upon reaction with goethite. The multiple regression equation, based only on MWAP and HIX parameters, explained 76% of the variance in amount of DOM sorbed. The results indicate that MWAP and HIX are important factors in controlling the sorption of DOM to mineral surfaces. Amendment with materials that release DOM of higher molecular weight and greater humification will result in enhanced initial sorption of DOM to soil solids, thereby contributing to accumulation of a larger soil organic C pool. [source] Volatile components of Thymus vulgaris L. from wild-growing and cultivated plants in JordanFLAVOUR AND FRAGRANCE JOURNAL, Issue 4 2007M. Hudaib Abstract The composition of the essential oil hydrodistilled from the aerial parts of Thymus vulgaris L. grown in Jordan has been determined by GC and GC,MS. Variations in oil composition and yield between cultivated and wild-growing plants collected from different localities, at different altitudes, have been also evaluated. Higher oil yields were observed in plants growing wild (3.7,5.6% of dried material) than in cultivated plants (1.1,2.0%), and those collected from the Mshaqar region, in the middle of Jordan and at the highest altitude, were the richest in oil (,5.4%). Generally, the oil was characterized by marked levels of phenolic monoterpenoids (mainly thymol and carvacrol) in the range 70.8,89.0%. High levels of the monoterpenoid hydrocarbons p -cymene (3.4,8.2%) and , -terpinene (1.6,7.7%) were also observed. Other major components were 1,8-cineole (up to 2.1%), , -thujone (up to 1.2%), camphor (up to 1.1%) and , -caryophyllene (0.2,2.8%). With the exception of plants growing wild in the Ramtha region, in the far north of Jordan, carvacrol was found as the principal phenol of all other oils (50.6,86.1%) and was dominant (>85%) in wild plant oils. The oil from Ramtha was characterized by the highest level of thymol (,63.8%) as the dominant phenol and was most abundant in p -cymene (8.2%), 1,8-cineole (2.1%) and , -terpinene (7.7%). In addition to assigning carvacrol or thymol chemotypes to the plant, the high content of active monoterpenoid phenols strongly suggests a potential use of Jordanian thyme oil in cough products and antimicrobial,herbal drug combinations. Copyright © 2007 John Wiley & Sons, Ltd. [source] Chemical variability of Artemisia vulgaris L. essential oils originated from the Mediterranean area of France and CroatiaFLAVOUR AND FRAGRANCE JOURNAL, Issue 5 2003I. Jerkovic Abstract The essential oils of Artemisia vulgaris L. originating from France and Croatia were subjected to detailed GC,MS analysis in order to determine possible similarities and differences between them and their chemical compositions, depending on the stage of development. Plant materials were collected in Provence (France) and Dalmatia (Croatia) at four different stages of development: vegetative (June), buds (July), ,owering (August) and seeding (September). Comparison of the chemical composition and content, depending on phenological stages in both localities, shows qualitative similarity, but also the noticeable difference in the amounts of most components. First, the chemical composition of main compounds is very different; Croatian oils are rich in hydrocarbons, which are absent or present in low amounts in French oils. Also, with regard to the yield of essential oils, the yields from Croatian oils were higher (0.09,0.61%) than these from French oil (0.04,0.15%) at each development stage, respectively. Copyright © 2003 John Wiley & Sons, Ltd. [source] Long-range foraging by the honey-bee, Apis mellifera L.FUNCTIONAL ECOLOGY, Issue 4 2000M. Beekman Abstract 1.,Waggle dances of honey-bees (Apis mellifera L.) were decoded to determine where and how far the bees foraged during the blooming of heather (Calluna vulgaris L.) in August 1996 using a hive located in Sheffield, UK, east of the heather moors. The median distance foraged was 6·1 km, and the mean 5·5 km. Only 10% of the bees foraged within 0·5 km of the hive whereas 50% went more than 6 km, 25% more than 7·5 km and 10% more than 9·5 km from the hive. 2.,These results are in sharp contrast with previous studies in which foraging distances were much closer to the hive. In May 1997 the mean foraging distance was 1 km, showing that long-range dancing is not the rule in Sheffield. 3.,The observed foraging distances described in this study may not be exceptional in a patchy environment where differences in patch size and patch quality are large. When travel distances to patches are large, distant patches can probably be utilized only by individuals that live in groups and recruit foragers to the patches found. Only then are the benefits of scouting for distant patches high enough to enable the exploitation of these patches. [source] Effects of elevated temperature and carbon dioxide on seed-set and yield of kidney bean (Phaseolus vulgaris L.)GLOBAL CHANGE BIOLOGY, Issue 8 2002P. V. Vara Prasad Abstract It is important to quantify and understand the consequences of elevated temperature and carbon dioxide (CO2) on reproductive processes and yield to develop suitable agronomic or genetic management for future climates. The objectives of this research work were (a) to quantify the effects of elevated temperature and CO2 on photosynthesis, pollen production, pollen viability, seed-set, seed number, seeds per pod, seed size, seed yield and dry matter production of kidney bean and (b) to determine if deleterious effects of high temperature on reproductive processes and yield could be compensated by enhanced photosynthesis at elevated CO2 levels. Red kidney bean cv. Montcalm was grown in controlled environments at day/night temperatures ranging from 28/18 to 40/30 °C under ambient (350 µmol mol,1) or elevated (700 µmol mol,1) CO2 levels. There were strong negative relations between temperature over a range of 28/18,40/30 °C and seed-set (slope, ,,6.5% °C,1) and seed number per pod (, 0.34 °C,1) under both ambient and elevated CO2 levels. Exposure to temperature >,28/18 °C also reduced photosynthesis (, 0.3 and ,,0.9 µmol m,2 s,1 °C,1), seed number (, 2.3 and ,,3.3 °C,1) and seed yield (, 1.1 and ,,1.5 g plant,1 °C,1), at both the CO2 levels (ambient and elevated, respectively). Reduced seed-set and seed number at high temperatures was primarily owing to decreased pollen production and pollen viability. Elevated CO2 did not affect seed size but temperature >,31/21 °C linearly reduced seed size by 0.07 g °C,1. Elevated CO2 increased photosynthesis and seed yield by approximately 50 and 24%, respectively. There was no beneficial interaction of CO2 and temperature, and CO2 enrichment did not offset the negative effects of high temperatures on reproductive processes and yield. In conclusion, even with beneficial effects of CO2 enrichment, yield losses owing to high temperature (> 34/24 °C) are likely to occur, particularly if high temperatures coincide with sensitive stages of reproductive development. [source] Influence of natural and controlled fermentations on , -galactosides, antinutrients and protein digestibility of beans (Phaseolus vulgaris L.)INTERNATIONAL JOURNAL OF FOOD SCIENCE & TECHNOLOGY, Issue 4 2008Emire Admassu Shimelis Summary The influence of natural fermentation (NF) and controlled fermentation (CF) in diminishing the content of antinutrients, , -galactosides and increments in in vitro protein digestibility was investigated. The dry bean (Phaseolus vulgaris) flour was the substrate used in this research study. A decrease in raffinose oligosaccharide, antinutritional components and pH was observed in both types of fermentation. The natural lactic fermentation of ground beans produced significant increase (P < 0.05) in protein digestibility. For all varieties of beans, raffinose concentration reduced significantly to an undetectable level after 96 h of NF. CF did not have any significant effect on the reduction of the , -galactosides content of the flours during fermentation. NF is an inexpensive method by which consumers can obtain good-quality protein. Both types of fermentation diminish antinutrients and improve the nutritional value of the bean flour, and indicate the potential to use bean flour as an ingredient for fabricated foods. [source] Effects of Deficit Irrigation and Salinity Stress on Common Bean (Phaseolus Vulgaris L.) and Mungbean (Vigna Radiata (L.) Wilczek) Grown in a Controlled EnvironmentJOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 4 2010M. Bourgault Abstract As water for irrigation purposes becomes increasingly scarce because of climate change and population growth, there is growing interest in regulated deficit irrigation (RDI) as a way to improve efficiency of water usage and farm productivity in arid and semi-arid areas. Salinity is also becoming an important problem in these same regions. Experiments were performed to investigate the effects of RDI and salt stress on two legumes crops, common bean (Phaseolus vulgaris L.) and mungbean (Vigna radiata (L.) Wilczek); previous work showed contrasting responses to RDI by these two crops under field conditions. The seed and biomass yields of both crops were reduced as a result of increasing water deficit stress; however, mungbean was able to maintain the same proportion of its biomass in reproductive structures and maintain its harvest index under stress, whereas common bean's decreased. In addition, photosynthesis in mungbean was higher than in common bean and higher at the same levels of transpiration. Finally, salinity stress did not affect the water potential, harvest index or the specific leaf weight of either crop. There were no interactions between salinity and crops or RDI levels, which suggest that the two crops do not differ in their response to salinity stress, and that RDI levels do not modify this response. [source] Evaluation of Common Bean for Drought Tolerance in Juana Diaz, Puerto RicoJOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 5 2009T. G. Porch Abstract Drought tolerance is an increasingly important trait in common bean (Phaseolus vulgaris L.) due to the reduction in water resources, a shift in production areas and increasing input costs. The objective of this study was to evaluate 29 genotypes for drought tolerance under drought stress (DS) and reduced stress treatments in Juana Diaz, Puerto Rico. The use of DS and reduced stress treatments facilitated the identification of drought tolerant germplasm that also had good yield potential under more optimal conditions. Based on the results of seed yield under DS and reduced stress conditions, and DS indices, including the geometric mean (GM), stress tolerance index (STI) and percent yield reduction (YR), genotypes were identified with greater yield potential under the tested environment. Based on average GM over the 2 years, the superior common bean genotypes identified were SEA 5, G 21212, A 686, SEN 21 and SER 21. These genotypes performed well in both years and under both treatment conditions and thus may serve as parents for DS improvement and genetic analysis. [source] Environmental and Genotypic Effects on Pod Characteristics Related to Common Bean QualityJOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 4 2004Dr A. M. De Ron Abstract A collection of 121 common bean (Phaseolus vulgaris L.) landraces from Spain and Portugal (Iberian Peninsula) was evaluated in six environments (three locations and 2 years). Significant differences among landraces were found for period of flowering, pod maturity and pod morphology (weight, length, width/thickness and curvature). Wide variation among landraces was revealed by the range of variation observed. Environmental effects were not significant although year × location and landrace × year × location interactions were significant for all traits studied except for pod curvature. Poor consistent expression across the different environments for most of the traits studied was expressed by the low values of constancy (C) with the exception of width/thickness indicating that pod shape (round or flat) was expressed regularly across different environments. Principal component analysis enhanced differences among environments affecting the performance of the bean landraces evaluated. Analysis by environment showed that 51 landraces were adapted to specific environments and only four of them had broad geographical adaptability with similar performance under different conditions. These results could be a starting point for selection of new inbred lines adapted to distinct environments with potential for the improvement of current snap bean cultivars. [source] Leaf Carbon Isotope Discrimination and its Relation with Qualitative Root Traits and Harvest Index in Sugar Beet (Beta vulgaris L.)JOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 5 2003J. T. Tsialtas Abstract Twenty commercial sugar beet varieties were grown at two sites in central Greece during the 2001 growing season (March to October), in a completely randomized block design with three replications. Carbon isotope discrimination (,) in sugar beet leaves was found to be affected by site and marginally by variety. No relation was found between fresh root yield and ,, but significant correlations were found between , and sucrose content (positive) and between , and root ,-amino-N (negative). Also, the harvest index, determined at one site, was negatively correlated with ,. [source] Plant,Water Relations of Kidney Bean Plants Treated with NaCl and Foliarly Applied GlycinebetaineJOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 2 2002C. M. L. Lopez Salinity is at present one of the most serious environmental problems influencing crop growth. It has been extensively demonstrated that salinity affects several physiological processes in the plant, including the plant,water relations of most salt-sensitive crops species. In this study, the effects of salinity on the plant,water relations of kidney bean (Phaseolus vulgaris L.) and the possibility that foliarly applied glycinebetaine improves these water relations are examined. Kidney bean plants were grown in a greenhouse and treated with 0, 30, 50 and 100 mM NaCl, combined with 0, 10 and 30 mM glycinebetaine in foliar applications. Increased salinity levels decreased stomatal conductance, photosynthetic rate, transpiration and leaf relative water content in the 30, 50 and 100 mM treatments relative to the control treatment. Glycinebetaine applications of 10 mM increased stomatal conductance at 50 mM NaCl, ameliorating significantly the effect of salinity on water relations through increases in the leaf relative water content. At 100 mM NaCl, 30 mM glycinebetaine applications in particular contributed to osmotic stress, and had an adverse effect on plants. Our experiment suggests that glycinebetaine can be used as an alternative treatment to reduce the effects of salt stress on the water relations of salt-sensitive plants, but only to limited salinity levels. Furthermore, the improvement in the water status of kidney beans was dose dependent, suggesting that the concentration of glycinebetaine essential for the survival of salt-sensitive plants is species specific and must be determined individually for each plant species. Pflanzen,Wasser-Beziehungen von NaCl-behandelten und mit Glycinbetain besprühten Blättern von Gartenbohnenpflanzen Versalzung ist zur Zeit eine der am meisten wirksamen Umweltprobleme im Hinblick auf das Wachstum von Kulturpflanzen. Es hat umfangreiche Untersuchungen gegeben, die Versalzungswirkungen in ihrem Einfluss auf zahlreiche physiologische Vorgänge in der Pflanze zu untersuchen; hierbei wurden auch die Pflanzen,Wasser-Beziehungen von hochsalzempfindlichen Pflanzenarten berück-sichtigt. In dieser Untersuchung wurden die Einflüsse der Versalzung auf die Pflanzen,Wasser-Beziehungen bei Buschbohnen (Phaseolus vulgaris L.) und die Möglichkeit über Blattbesprühungen mit Glycinbetain die Wasser-Beziehungen zu verbessern, untersucht. Die Buschbohnen wurden im Gewächshaus angezogen und mit 0, 30, 50 mM NaCl in Kombination mit 0, 10, 30 mM Glycinbetain Blattbehandlungen angezogen. Eine Erhöhung der Versalzung führte zu einer Abnahme der stomatären Konduktanz, der Photosyntheserate, der Transpiration und des relativen Blattwassergehaltes bei den Behandlungen mit 30, 50 und 100 mM im Vergleich zur Kontrolle. Glysinbetainanwendungen von 10mM erhöhten die stomatäre Konduktanz bei 50 mM NaCl und verbesserten signifikant den ungünstigen Einfluss der Versalzung auf die Wasser-Beziehungen über eine Erhöhung des relativen Blattwassergehaltes. Verwendung von 100 mM NaCl und 30 mM GB trug zu dem osmotischen Streß durch Versalzung bei und hatten einen ungünstigen Einfluss auf die Pflanzen. Unser Experiment weist darauf hin, dass Glycinbetain eine alternative Möglichkeit ist, um die Einflüsse des Salzstresses auf die Wasser-Beziehungen von salzempfindlichen Pflanzen abzuschwächen; es bestehen aber Begrenzungen bezüglich des Versalzungsgrades, bei denen eine günstige Wirkung nachgewiesen werden kann. Ausserdem ist die Verbesserung im Wasserzustand der Buschbohnen von der Anwendungsstärke abhängig, so dass die Konzentration von GB wesentlich für das Überleben der salzempfindlichen Pflanzenart spezifisch ist und für jede Pflanzenart untersucht werden. [source] Effects of Interactions of Moisture Regime and Nutrient Addition on Nodulation and Carbon Partitioning in Two Cultivars of Bean (Phaseolus vulgaris L.)JOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 4 2001T. Boutraa Major limitations of bean (Phaseolus vulgaris L.) production in arid and semiarid regions are lack of moisture and low soil fertility. An experiment was conducted to determine the effects of soil moisture and N : P : K (20 : 10 : 10) fertilizer on root and shoot growth of two cultivars of bean: cv. Carioca, an indeterminate Brazilian landrace, and cv. Prince, a determinate cultivar grown in Europe. Carioca appears generally stress-tolerant while Prince is intolerant. Seedlings were grown in pots of non-sterile soil at 30, 60 or 90 % field capacity (FC), and given 0, 0.1 or 1 g (kg soil),1 of compound fertilizer. The soil contained a population of effective Rhizobium. Growth of both cultivars was greatest in the high moisture and high nutrient treatments. Root fractions were highest at low nutrient supply; the effect of water was not significant. Leaf fraction decreased as root fraction increased. Numbers of nodules were highest at high and intermediate moisture when no fertilizer was applied. Numbers were lowest at 30 % FC and at the highest fertilizer rate. Masses of nodules and fractions followed the same pattern. Decreasing water regime reduced the relative growth rate (RGR) of Prince, while Carioca maintained high RGR at unfavourable conditions of water and nutrients. Net assimilation rates (NAR) were unaffected by nutrient addition, and reduced by low moisture regime. Water use efficiencies (WUEs) were reduced by water stress but increased by nutrient deficiency. The water utilization for dry matter production was optimal at 60 % FC. Einflüsse der Interaktionen von Bodendenfeuchte und Düngung auf die Knöllchenbildung und Kohlenstoff verteilung bei zwei Bohnenkultivaren (Phaseolus vulgaris L.) Der begrenzende Hauptfaktor der Bohnenproduktion in ariden und semiariden Regionen sind der Feuchtigkeitsmangel und die Bodenfruchbarkeit. Es wurde ein Experiment durchgeführt, um die Wirkungen des Bodenwassers und von N : P : K (20 : 10 : 10) Dünger auf das Wurzel- und Sproßwachstum an zwei Kultivaren von Bohnen (Phaseolus vulgaris L. cv. Carioca, eine brasilianischen, indeterminierte Landsorte und cv. Prince, eine determinierter in Europa angebauter Kultivar) zu untersuchen. Carioca erscheint grundsätzlich streßtoleranter im Vergleich zu Prince. Die Sämlinge wurden in Gefäßen mit nichtsterilisiertem Boden unter Feldkapazitäten von 30,60 oder 90 % mit 0, 0,1 oder 1 g eines Volldüngers angezogen. Der Boden enthielt eine Population von wirksamem Rhizobium. Das stärkste Wachstum wurde bei beiden Kultivaren unter dem Einfluß des höchsten Feuchtigkeitsgehaltes und der höchsten Düngermenge gefunden. Der Wurzelanteil war bei der geringen Düngermenge am niedrigsten. Der Einfluß der Bodenfeuchtigkeit war nicht signifikant. Der Blattanteil nahm mit zunehmendem Wurzelanteil ab. Die Anzahl der Knötchen war bei hoher und mittlerer Bodenfeuchte und ohne Düngeranwendung am höchsten. Die Anzahl war am geringsten bei 30 % FC und der höchsten Düngermenge. Die Knötchenmasse und ihr Anteil reagierte entsprechend. Abnehmende Bodenfeuchte reduzierte die relative Wachtumsrate (RGR) von Prince, während Carioca einen hohen RGR auch bei ungünstigen Bedingungen bezüglich Wasser und Düngung behielt. Die Nettoassimilationsraten wurden durch die Düngung nicht beeinflußt; sie gingen bei geringer Bodenfeuchte zurück. Die Wassernutzungseffiziens (WUE) wurde bei Wasserstreß reduziert, nahm aber bei Düngermangel zu. Die Wassernutzung für die Trockenmasseproduktion war bei 60 % Feldkapazität am höchsten. [source] Within-sex density dependence and population dynamics of red squirrels Sciurus vulgarisJOURNAL OF ANIMAL ECOLOGY, Issue 1 2004Luc A. Wauters Summary 1Social organization and dispersal of red squirrels (Sciurus vulgaris L.) differ between sexes, and intrasexual competition is intense. Therefore, we predicted that demographic parameters should be gender-specific: that is density-dependent factors will be more strongly related to density of the same sex than to density of the opposite sex. We studied the relative importance of within- and between-sex density-dependent factors and of density-independent factors (habitat type, food abundance, winter temperature) on different demographic parameters, in two populations in northern Belgium. 2Spring density of males was positively correlated with tree-seed abundance in the previous year, but this was not the case for females. None of the population parameters we measured differed between habitats, indicating that the same density-dependent and density-independent mechanisms prevailed in coniferous and deciduous habitat. 3Within each sex, we found several demographic parameters that were dependent on the densities of the same sex; however, none of these parameters was found to be dependent on the density of the opposite sex. 4Reproductive rate increased with food abundance and decreased with female density. Adult survival of females decreased with female density in autumn,winter, while survival of adult males in spring,summer increased with the size of the previous year's seed crop. 5Immigration rate of males was higher in spring than in autumn, and spring immigration increased with food abundance. Male recruitment rate, in both seasons, increased with food abundance, but was male density dependent. However, spring,summer loss rates also increased when food supplies were good, suggesting that despite high food availability, emigration of juvenile and subadult males increased when intrasexual competition was intense. Recruitment rate of females decreased with increasing female density. After a good seed crop, more subadult females dispersed, but their settlement success (recruitment) was lower at high female density. 6Seed crop size positively affected red squirrel densities through increased reproduction, immigration and adult survival of males, but density-dependent reproduction and within-sex density-dependent recruitment of locally born juveniles and dispersing subadults limit the fluctuations in numbers and regulate densities in winter,early spring, as well as in summer. [source] Comparative genomics-guided loop-mediated isothermal amplification for characterization of Pseudomonas syringae pv. phaseolicolaJOURNAL OF APPLIED MICROBIOLOGY, Issue 3 2009X. Li Abstract Aims:, To design and evaluate a loop-mediated isothermal amplification (LAMP) protocol by combining comparative genomics and bioinformatics for characterization of Pseudomonas syringae pv. phaseolicola (PSP), the causal agent of halo blight disease of bean (Phaseolus vulgaris L.). Methods and Results:, Genomic sequences of Pseudomonas syringae pathovars, P. fluorescens and P. aeruginosa were analysed using multiple sequence alignment. A pathovar-specific region encoding pathogenicity-related secondary metabolites in the PSP genome was targeted for developing a LAMP assay. The final assay targeted a polyketide synthase gene, and readily differentiated PSP strains from other Pseudomonas syringae pathovars and other Pseudomonas species, as well as other plant pathogenic bacteria, e.g. species of Pectobacterium, Erwinia and Pantoea. Conclusion:, A LAMP assay has been developed for rapid and specific characterization and identification of PSP from other pathovars of P. syringae and other plant-associated bacteria. Significance and Impact of the Study:, This paper describes an approach combining a bioinformatic data mining strategy and comparative genomics with the LAMP technology for characterization and identification of a plant pathogenic bacterium. The LAMP assay could serve as a rapid protocol for microbial identification and detection with significant applications in agriculture and environmental sciences. [source] SOME PHYSICOMECHANICAL AND NUTRITIONAL PROPERTIES OF BARBERRY (BERBERIS VULGARIS L.) FRUITSJOURNAL OF FOOD PROCESS ENGINEERING, Issue 4 2009MEHMET AKBULUT ABSTRACT The barberry (Berberis vulgaris L.) fruits growing wild in Turkey were analyzed for some physical (dimensions, geometric mean diameter, sphericity, bulk density, fruit density, volume, terminal velocity, hardness and porosity) and chemical (moisture, reducing sugar, ascorbic acid, total anthocyanin and phenolics, crude protein, crude oil, crude energy, crude fiber, ash, pH, acidity, alcohol-soluble extract and color) properties. Mineral content of barberry fruits growing wild in Turkey was determined by inductively coupled plasma-atomic emission spectrometry. The average pulp mass ratio, thickness, width, length, mass, volume, geometric mean diameter, sphericity and projected area were measured as 75.59%, 3.51 mm, 3.32 mm, 7.69 mm, 0.07 g, 71 mm3, 4.46 mm, and 0.58 and 0.2535 cm2, respectively. The energy, reducing sugar, protein, cellulose, oil, ash, acidity, ascorbic acid, total phenolics, total anthocyanin and soluble solid matter values of barberry fruits were established as 69.25 kcal/g, 6.52%, 10.32%, 9.42%, 0.84%, 1.12%, 3.10%, 256.48 mg/kg, 789.32 mg/100 g, 931.05 mg/kg and 19.4%, respectively. It is very important to evaluate the technological properties of equipment used in harvesting, transportation, storage and processing of fresh fruits. Also, the information supplied on the proximate composition of the barberry fruit is highly beneficial for human nutrition. PRACTICAL APPLICATIONS The chemical properties such as ascorbic acid, anthocyanin and phenolic compounds, reducing sugar, crude protein, crude fiber, crude oil, ash and mineral contents of barberry fruits are mostly important for human nutrition and processing. In addition, it is very important to establish the physical properties of equipment used in harvesting, transportation, storage and processing of fresh fruits. However, further studies are necessary to understand the physical properties for equipment design and to provide necessary information for the use of wild edible fruits. [source] EFFECT OF EXTRUSION COOKING AND SODIUM BICARBONATE ADDITION ON THE CARBOHYDRATE COMPOSITION OF BLACK BEAN FLOURSJOURNAL OF FOOD PROCESSING AND PRESERVATION, Issue 2 2002JOSE DE J. BERRIOS ABSTRACT Extrusion cooking and chemical leavening agents such as sodium bicarbonate (NaHCO3), may induce changes in carbohydrate fractions of extruded black bean (Phaseolus vulgaris L.) flours. Bean flours at 20% moisture, with NaHCO3 added at levels from 0.0 to 2.0%, were extruded at a screw speed of 200 rpm. The temperature profile ranged from 23 to 160C. Extruded bean flours with 0.1 to 0.4% added NaHCO3 were selected for sugar analyses based on color and flavor acceptability. The major sugars determined in the bean samples were galactose (0.10%), sucrose (2.08%), and stachyose (2.00%). Extruded samples had an increase in total sugars. Also, an increase in soluble fiber and a decrease of insoluble fiber fractions were observed. Sucrose was the only free sugar which concentration decreased consistently as a result of extrusion processing. Extrusion conditions and the selected levels of NaHCO3 used in this study did not significantly change the oligosaccharide content of the black bean flours. [source] Impact of Thermal Treatment on Color and Pigment Pattern of Red Beet (Beta vulgaris L.) PreparationsJOURNAL OF FOOD SCIENCE, Issue 6 2004K.M. Herbach ABSTRACT: The impact of heating at 85°C during 8 h on overall color and betalain pattern of red beet (Beta vulgaris L. ssp. vulgaris) juice was investigated. Although the hue angle of 358° in fresh juice was indicative of the typical red-purple appearance, heating for 8 h induced an unexpected shift to 62° resulting in a yellow-orange solution. To monitor the underlying structural alterations of betalains, a new high-performance liquid chromatography separation compatible with mass spectrometry was developed. Applying this method, 2 novel yellow neobetanin structures and 2 orange-red betanin degradation products were preliminarily identified, and neobetanin formation resulting from heat exposure was proven for the 1st time. These 5 compounds were held responsible for the orange shift of red beet juice during thermal treatment. The relevance of these findings for industrial beet processing was demonstrated by comparison of pigment patterns of heated red beet juice samples and a commercial concentrate. On the basis of these results, a scheme for the thermal degradation of betanin is proposed. [source] In-vitro anti-inflammatory effect of Eucalyptus globulus and Thymus vulgaris: nitric oxide inhibition in J774A.1 murine macrophagesJOURNAL OF PHARMACY AND PHARMACOLOGY: AN INTERNATI ONAL JOURNAL OF PHARMACEUTICAL SCIENCE, Issue 2 2004E. Vigo ABSTRACT It is well known that nitric oxide (NO) plays an important role in the pathogenesis of inflammatory diseases. Eucalyptus globulus Labill. and Thymus vulgaris L. have been used in traditional medicine in the treatment of bronchitis, asthma and other respiratory diseases. The present study focuses on the effects of these two extracts on NO production induced by lipopolysaccharide (LPS) and interferon-, (IFN-,) in the murine macrophage cell line J774A.1. In addition, cell viability, scavenging activity and inducible nitric oxide synthase (iNOS) mRNA expression were evaluated. E. globulus and T. vulgaris extracts significantly inhibited the enhanced production of NO induced by LPS and IFN-, in a dose-dependent manner. Treatment with these two extracts did not reduce cell viability at any dose used. Both plant extracts showed significant scavenging of NO radicals released by an NO donor, PAPANONOate. Results also show that pre-treatment with E. globulus and T. vulgaris extracts significantly inhibits iNOS mRNA expression. This study thus suggests that the inhibition of net NO production by these two extracts may be due to their NO scavenging activity and/or their inhibitory effects on iNOS gene expression. [source] Sodium-induced calcium deficiency in sugar beet during substitution of potassium by sodiumJOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, Issue 2 2009Abdul Wakeel Abstract Functions of sodium (Na+) and potassium (K+) are closely associated. In some crops, Na+ is able to prevent or reduce considerably the occurrence of K+ deficiency. Sugar beet (Beta vulgaris L.) is a natrophilic crop, and positive effects of Na+ applications on yield were observed when K+ was sufficiently supplied. However, it is not known which specific function of K+ can limit the growth of sugar beet when K+ is substituted by an equivalent amount of Na+. Therefore, K+ substitution by Na+ was investigated for sugar beet in hydroponics. Surprisingly, no K+ -deficiency symptoms were observed. However, calcium (Ca2+) concentrations in the leaves were significantly decreased. Moreover, Ca2+ uptake and translocation through xylem sap were reduced in Na+ -treated plants. It is concluded that Ca2+ uptake by roots and its translocation via xylem sap primarily limit the possibility of K+ substitution by Na+ in sugar beet nutrition. [source] Evaluation and standardisation of small-scale canning methods for small white beans (Phaseolus vulgaris L.) canned in tomato sauceJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 7 2006Daleen van der Merwe Abstract Canning and evaluation procedures should be standardised to ensure that beans selected, based on canning quality, meet the requirements set by the market and processors. For the purpose of evaluating the canning qualities of small white beans in tomato sauce, three small-scale canning techniques were evaluated, one of which was found to deliver a product with quality parameters similar to those of international standards. Using South African small white bean cultivars and the selected method, Teebus, the cultivar used by industry as the standard to indicate acceptable canning quality, displayed better visual appearance and less split beans than with the other two methods. The percentage washed drained weight and texture values of Teebus were also in agreement with international standards. The evaluation procedure for the small white beans after canning was also optimised, by comparing two procedures, which identified texture, visual appearance (scale 1 to 10), splits (scale 1 to 10), hydration coefficient, clumping, size and colour as the statistically most suitable quality parameters. With the aid of the developed method, it was possible to define standard values for South African ,choice' and ,standard' grade beans, which previously was based only on ,visual inspection' by a trained inspection panel. Copyright © 2006 Society of Chemical Industry [source] Seasonal patterns of sucrose concentration in relation to other quality parameters of sugar beet (Beta vulgaris L.)JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 1 2006Christine Kenter Abstract The chemical composition of sugar beet is the most important parameter affecting its processing. Sugar factories require beet with high concentrations of sucrose and low concentrations of melassigenic substances to maximise the amount of extractable sugar. In order to plan the processing campaign, forecasts of root and sugar yield by prediction models are possible but there are no means to predict the technical quality of the beet. In the present study, the seasonal development and physiological relationships of different parameters of sugar beet quality were analysed. In order to estimate possibilities for quality forecasts, the concentrations of beet quality variables in October were correlated with corresponding quality measurements in late summer and to weather variables during the growing season by linear regressions. In 2000 and 2001, 27 field trials were conducted on commercial farm fields in all sugar beet growing areas in Germany. From June to October, sequential samples were taken every 4 weeks and the concentrations of sucrose, potassium, sodium, total soluble nitrogen, ,-amino nitrogen, nitrate, betaine, reducing sugars and marc in the beet were determined. The sucrose concentration increased progressively until the final harvest date in autumn, whereas the concentrations of the melassigenic substances decreased markedly until late summer and remained fairly constant as the season progressed. Marc concentration was the most stable of the parameters analysed. The sucrose concentration was positively correlated with the concentrations of dry matter, betaine and marc, but negatively with nitrate concentration and leaf yield throughout the season. The correlation between the concentrations of sucrose and nitrogenous compounds measured in summer and their final concentrations in autumn was rather weak. However, it was close for potassium, sodium and marc and a satisfactory prediction of their final concentrations was possible by the end of August. Based on weather data, beet quality was not predictable. Therefore, it seems to be difficult to integrate beet quality parameters into prediction models. Copyright © 2005 Society of Chemical Industry [source] Effects of temporal and spatial variations in food supply on the space and habitat use of red squirrels (Sciurus vulgaris L.)JOURNAL OF ZOOLOGY, Issue 2 2000Peter W. W. Lurz Abstract In non-native conifer plantations characterized by strong spatial and temporal variations in the availability of tree seeds in Spadeadam Forest, northern England, the home range and habitat use of red squirrels Sciurus vulgaris was very flexible. Males tended to have much larger home ranges than females and core-areas of most breeding females seemed mutually exclusive. Adult female red squirrels were found to increase their home range and core-area size in forest patches where food was less abundant. Home-range size was significantly related to home-range quality and the extent of overlap by other females. In contrast with high-quality continuous conifer forests: (1) a considerable proportion of adult males and females at Spadeadam shifted home range, (2) both sexes had much larger home ranges than reported from other habitats in Britain or Belgium. Many ranges were multinuclear, particularly from January onwards, when supplies of seeds become depleted through consumption and seed shed. Squirrels tracked the availability of conifer seeds (lodgepole pine cones throughout the study, Norway spruce cones in spring 1992 and Sitka spruce cones in autumn 1993) and intensively used several non-adjacent activity centres in temporally food-rich patches. Consequently, habitat preference changed markedly with time. The squirrels seemed to maximize nitrogen intake and to avoid the smaller seeds when possible. This resulted in an overall preference for a mixed diet of lodgepole pine and spruce seeds and avoidance of Sitka spruce seeds when Norway spruce seeds were available. These results lend support to the hypothesis of Ostfeld (1985) that when food is sparse and patchily distributed, females should develop intrasexual territoriality, concentrating their activity in food-rich patches, while males should be non-territorial and adapt their space use to the distribution of females. [source] Scanning electron microscopy applied to seed-borne fungi examinationMICROSCOPY RESEARCH AND TECHNIQUE, Issue 7 2009Marcelo De Carvalho Alves Abstract The aim of this study was to test the standard scanning electron microscopy (SEM) as a potential alternative to study seed-borne fungi in seeds, by two different conditions of blotter test and water restriction treatment. In the blotter test, seeds were subjected to conditions that enabled pathogen growth and expression, whereas the water restriction method consisted in preventing seed germination during the incubation period, resulting in the artificial inoculation of fungi. In the first condition, seeds of common bean (Phaseolus vulgaris L.), maize (Zea mays L.), and cotton (Gossypium hirsutum L.) were submitted to the standard blotter test and then prepared and observed with SEM. In the second condition, seeds of cotton (G. hirsutum), soybean (Glycine max L.), and common bean (P. vulgaris L.) were, respectively, inoculated with Colletotrichum gossypii var. cephalosporioides, Colletotrichum truncatum, and Colletotrichum lindemuthianum by the water restriction technique, followed by preparation and observation with SEM. The standard SEM methodology was adopted to prepare the specimens. Considering the seeds submitted to the blotter test, it was possible to identify Fusarium sp. on maize, C. gossypii var. cephalosporioides, and Fusarium oxysporum on cotton, Aspergillus flavus, Penicillium sp., Rhizopus sp., and Mucor sp. on common bean. Structures of C. gossypii var. cephalosporioides, C. truncatum, and C. lindemuthianum were observed in the surface of inoculated seeds. Microsc. Res. Tech., 2009. © 2009 Wiley-Liss, Inc. [source] Characterization of a novel Toll/interleukin-1 receptor (TIR)-TIR gene differentially expressed in common bean (Phaseolus vulgaris cv. Othello) undergoing a defence response to the geminivirus Bean dwarf mosaic virusMOLECULAR PLANT PATHOLOGY, Issue 2 2007YOUNG-SU SEO SUMMARY Common bean (Phaseolus vulgaris L.) cultivar (cv.) Othello develops a hypersensitive response-associated vascular resistance to infection by Bean dwarf mosaic virus (BDMV), a single-stranded DNA virus (genus Begomovirus, family Geminiviridae). A PCR-based cDNA subtraction approach was used to identify genes involved in this resistance response. Eighteen clones, potentially involved with BDMV resistance, were identified based upon being up-regulated in BDMV-infected tissues and/or having sequence similarity with known resistance-associated genes. Analysis of these clones revealed potential genes involved in pathogen defence, including pathogenesis-related protein genes and resistance gene analogues (RGAs). Further characterization of one RGA, F1-10, revealed that it encodes a predicted protein with a double Toll/interleukin-1 receptor (TIR) motif. Full-length (F1-10) and spliced (F1-10sp) forms of the RGA were strongly up-regulated in BDMV-infected cv. Othello hypocotyl tissues by 4 days post-inoculation, but not in equivalent mock-inoculated tissues. In agroinfiltration experiments, F1-10, but not F1-10sp, mediated resistance to BDMV in the susceptible common bean cv. Topcrop. By contrast, transgenic Nicotiana benthamiana lines expressing F1-10 or F1-10sp were not resistant to BDMV. Interestingly, when these transgenic lines were inoculated with the potyvirus Bean yellow mosaic virus, some F1-10 lines showed a more severe symptom phenotype compared with non-transgenic control plants. Based on these findings, F1-10 was named: Phaseolus vulgaris VIRUS response TIR-TIR GENE 1 (PvVTT1). [source] Evaluation of the toxicity of 17 essential oils against Choristoneura rosaceana (Lepidoptera: Tortricidae) and Trichoplusia ni (Lepidoptera: Noctuidae),PEST MANAGEMENT SCIENCE (FORMERLY: PESTICIDE SCIENCE), Issue 10 2010Cristina M Machial Abstract BACKGROUND: The obliquebanded leafroller, Choristoneura rosaceana Harris, and the cabbage looper, Trichoplusia ni Hübner, are serious fruit and vegetable pests requiring multiple insecticide applications per year. To reduce non-target exposure to hazardous insecticides and to curb resistance development, alternative controls are required. Accordingly, a selection of 17 essential oils was screened against both lepidopteran pests, and the influence of azinphos-methyl resistance on essential oil toxicity to C. rosaceana was studied. RESULTS: Of the 17 essential oils screened, patchouli oil (Pogostemon cablin Benth.) and thyme oil (Thymus vulgaris L.) were selected for further testing against C. rosaceana, whereas patchouli oil, garlic oil (Allium sativum L.) and lemongrass oil (Cymopogon nardus L.) were selected for further testing against T. ni. LC50 and LD50 values confirmed that patchouli oil was the most toxic to C. rosaceana larvae, with LC50 = 2.8 µL mL,1 and LD50 = 8.0 µg insect,1. Garlic oil was the most toxic oil to T. ni larvae with LC50 = 3.3 µL mL,1 and LD50 = 22.7 µg insect,1, followed by patchouli oil and lemongrass oil. Azinphos-methyl-resistant leafrollers were 1.5-fold more tolerant to patchouli oil and 2.0-fold more tolerant to thyme oil. CONCLUSIONS: Based on these results, patchouli oil and other essential oils have sufficient efficacy to be considered as components of an essential oil-based insecticide that targets these lepidopteran pests. Copyright © 2010 Crown in the right of Canada. Published by John Wiley & Sons, Ltd [source] Estimation of plasmalemma conductivity to ascorbic acid in intact leaves exposed to ozonePHYSIOLOGIA PLANTARUM, Issue 4 2000Irina Bichele To establish the capacity of the leaf mesophyll plasmalemma of Phaseolus vulgaris L. to supply ascorbate (ASC) into the cell wall by simple diffusion, a method for calculating plasmalemma diffusional conductivity to ascorbic acid (AA) in intact leaves was evaluated. The core of the approach is that in the presence of a sink for ascorbate in the cell wall, cell wall total ascorbic acid concentration [TAA]cw (=[ASC]cw+[AA]cw) reaches zero at some positive whole-leaf total ascorbic acid concentration [TAA]l. It is shown that [TAA]l at [TAA]cw=0 is proportional to the sink for ASC in the cell wall and the reciprocal of plasmalemma conductivity. The predicted proportional relationship between [TAA]cw and [TAA]l was confirmed by decreasing TAA levels in leaves through predarkening. Furthermore, increasing the sink intensity for ASC in the cell wall by the acute exposure of leaves to 450 nmol ozone mol,1 during re-illumination, [TAA]cw reached zero at 2.7-fold higher [TAA]l than without ozone, and the slope of the relationship increased twofold. Plasmalemma diffusional conductivities to AA of 2.9×10,6 and 1.8×10,6 m s,1, needed to maintain [TAA]cw at the observed level, were calculated from the increase in [TAA]l at [TAA]cw=0 and from the two different estimates of the sink for ASC. A value of 1.3×10,6 m s,1 was calculated on the basis of the oil-water distribution coefficient for TAA. It is concluded that the demand for ASC in the mesophyll cell wall of the investigated leaves could be met by simple diffusion of AA through the plasmalemma. From the measured increase in the slope of the relationship [TAA]cw versus [TAA]l, an increase in the cell wall pH of 0.3 units was estimated under the influence of ozone. [source] Glutathione S-transferases and malondialdehyde in the liver of NOD mice on short-term treatment with plant mixture extract P-9801091PHYTOTHERAPY RESEARCH, Issue 4 2003R. Petlevski Abstract Changes in the concentration of glutathione S-transferases (GSTs) and malondialdehyde (MDA) were assessed in the liver of normal and diabetic NOD mice with and without treatment with the plant extract P-9801091. The plant extract P-9801091 is an antihyperglycaemic preparation containing Myrtilli folium (Vaccinium myrtillus L.), Taraxaci radix (Taraxacum of,cinale Web.), Cichorii radix (Cichorium intybus L.), Juniperi fructus (Juniperus communis L.) , Centaurii herba (Centaurium umbellatum Gilib.), Phaseoli pericarpium (Phaseolus vulgaris L.), Millefolii herba (Achillea millefolium L.), Mori folium (Morus nigra L.), Valerianae radix (Valeriana of,cinalis L.) and Urticae herba et radix (Urtica dioica L). Hyperglycaemia in diabetes mellitus is responsible for the development of oxidative stress (via glucose auto-oxidation and protein glycation), which is characterized by increased lipid peroxide production (MDA is a lipid peroxidation end product) and/or decreased antioxidative defence (GST in the liver is predominantly an , enzyme, which has antioxidative activity). The catalytic concentration of GSTs in the liver was signi,cantly reduced in diabetic NOD mice compared with normal NOD mice (p < 0.01), while the concentration of MDA showed a rising tendency (not signi,cant). The results showed that statistically signi,cant changes in antioxidative defence occurred in the experimental model of short-term diabetes mellitus. A 7-day treatment with P-9801091 plant extract at a dose of 20 mg/kg body mass led to a signi,cant increase in the catalytic concentration of GSTs in the liver of diabetic NOD mice (p < 0.01) and a decrease in MDA concentration (not signi,cant), which could be explained by its antihyperglycaemic effect. Copyright © 2003 John Wiley & Sons, Ltd. [source] On the mechanism of rejuvenation of ageing detached bean leaves by low-concentration stressorsPLANT BIOLOGY, Issue 2 2009P. Nyitrai Abstract The effect of low concentrations of some stress-inducing compounds of different toxicity and chemical nature, such as Cd and Pb salts or DCMU, was investigated on the senescence of chloroplasts in detached primary leaves of bean (Phaseolus vulgaris L.). After 1 week of senescence followed by root development from the petiole, these agents stimulated chlorophyll accumulation and photosynthetic activity (14CO2 fixation) as compared to the control, thus inducing rejuvenation. Low-concentration stressors increased the level of active cytokinins in roots and leaves during the treatment, as monitored by the Amaranthus betacyanin bioassay and high-pressure liquid chromatography. The lithium ion, an inhibitor of the PIP2 -IP3/DAG signal transduction pathway, abolished the stimulating effect of stressors, both in roots (retarding cytokinin synthesis) and consequently also in leaves (reducing cytokinin-dependent chlorophyll accumulation). This suggests the involvement of the PIP2 -IP3/DAG signal transduction pathway in generation of these consecutive organ-specific responses. [source] Effect of Salt Stress on Carbon Metabolism and Bacteroid Respiration in Root Nodules of Common Bean (Phaseolus vulgaris L.)PLANT BIOLOGY, Issue 4 2000A. Ferri Abstract: In the present work, we examined the effect of salinity on growth, N fixation and carbon metabolism in the nodule cytosol and bacteroids of Phaseolus vulgaris, and measured the O2 consumption by bacteroids incubated with or without the addition of exogenous respiratory substrates. The aim was to ascertain whether the compounds that accumulate under salt stress can increase bacteroid respiration and whether this capacity changes in response to salinity in root nodules of Phaseolus vulgaris. The plants were grown in a controlled environment chamber, and 50, 100 mM or no NaCl (control) was added to the nutrient solution. Two harvests were made, at the vegetative growth period and at the beginning of the reproductive period. The enzyme activities in the nodule cytosol were reduced by the salt treatments, while in the bacteroid cytosol the enzyme activities increased at high salt concentrations at the first harvest and for ADH in all treatments. The data presented here confirm that succinate and malate are the preferred substrates for bacteroid respiration in common bean, but these bacteroids may also utilize glucose, either in control or under saline conditions. The addition of proline or lactate to the incubation medium significantly raised oxygen consumption in the bacteroids isolated from plants treated with salt. [source] | |||||||||||||||||||