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Tryptophan Metabolites (tryptophan + metabolite)

Distribution by Scientific Domains
Medical Sciences40%
Chemistry40%

Selected Abstracts

New Tryptophan Metabolites from Cultures of the Lipophilic Yeast Malassezia furfur

CHEMINFORM, Issue 46 2005
Bernhard Irlinger
Abstract For Abstract see ChemInform Abstract in Full Text. [source]

Understanding the role of tryptophan and serotonin metabolism in gastrointestinal function

NEUROGASTROENTEROLOGY & MOTILITY, Issue 12 2009
D. Keszthelyi
Abstract, Tryptophan is the precursor of a wide array of metabolites, which are involved in a variety of aspects of human nutrition and metabolism. Accumulating evidence suggests a role of tryptophan metabolites, especially serotonin (5-hydroxytryptamin) in intestinal (patho) physiology, although mechanisms of action are still poorly understood. Alterations of serotonin metabolism may give rise to gastrointestinal dysfunction. Recently, it has been postulated that other metabolites of tryptophan, mostly of the kynurenine pathway, also play a role in regulating gut function. This review analyses the current knowledge of the interrelationship between tryptophan metabolic pathways and summarizes the existing scientific evidence regarding the role of tryptophan metabolites in intestinal function and in the pathogenesis of gastrointestinal diseases. [source]

Development of a fluorimetric detection method for cinnabarinic acid using ortho -tolyl hydrazine as the derivatization reagent

BIOMEDICAL CHROMATOGRAPHY, Issue 3 2010
Hideaki Iizuka
Abstract A fluorimetric detection method for one of the tryptophan metabolites, cinnabarinic acid (CA), which has recently been reported to have the ability to induce apoptosis in thymocytes, was developed using o -tolyl hydrazine (TH) as the derivatization reagent. The carbonyl group at position 3 in CA was tagged with the hydrazino moiety of TH at 100°C for 30 min, and the generated derivative, CA tagged with TH, fluoresced at 412 nm with a 316 nm excitation wavelength. The CA tagged with TH was separated on a reversed-phase HPLC and detected fluorometrically. The relative standard deviation was in the range of 1.1,8.9% (n = 3), and the detection limit was approximately 12?fmol (signal-to-noise ratio, 3). The proposed HPLC method can be useful for the sensitive detection of CA. Copyright © 2009 John Wiley & Son, Ltd. [source]

KYNURENINE METABOLITES AND INFLAMMATION MARKERS IN DEPRESSED PATIENTS TREATED WITH FLUOXETINE OR COUNSELLING

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, Issue 4 2009
Gillian M Mackay
SUMMARY 1Depression could result from changes in tryptophan availability caused by activation of the kynurenine pathway as a result of inflammation. In the present study, we examined patients newly diagnosed with depression to determine whether kynurenines and related factors change in parallel with improvements in mood. 2Concentrations of 5-hydroxytryptamine (5-HT; serotonin), 5-hydroxyindoleacetic acid (5-HIAA), oxidized tryptophan metabolites, brain-derived neurotrophic factor (BDNF) and inflammatory mediators (interleukin (IL)-2, C-reactive protein (CRP), neopterin) were measured in peripheral blood during an 18 week period of treatment with fluoxetine, fluoxetine plus tri-iodothyronine (T3) or psychiatric counselling. 3The results showed significant improvements in mood, with reduced 5-HT concentrations in patients given fluoxetine and a rise in plasma tryptophan in patients given counselling or fluoxetine and T3. The addition of T3 to the fluoxetine regimen appeared to slow recovery from depression, although the use of T3 was associated with a fall in thyroxine concentrations. Changes in 5-HT concentrations did not correlate with psychiatric scores and were seen only in drug-treated groups, not those given counselling. There were no associated changes in absolute concentrations of kynurenines, BDNF, CRP, neopterin or IL-2. With fluoxetine treatment, there were correlations between the concentrations of kynurenine metabolites and the psychiatric rating scores, whereas no correlations were found with BDNF or inflammatory markers. 4It is concluded that depression scores are largely independent of inflammatory status, but kynurenine metabolism may be related to the degree of depression after fluoxetine treatment. [source]

Filmy channel microchip with amperometric detection

ELECTROPHORESIS, Issue 22 2009
Wei Wang
Abstract In this article, a new type of microchip with filmy channels and a sample-injection fracture is introduced. Unlike commercial microchip, new microchip possessed filmy channel with width 2,3,mm. The effective cooling ability made filmy channel microchip restrain the generation of Joule heat even under electric field of 588,V/cm. Moreover, wider channel could be more easily modified to prevent the absorption of samples, load more samples and result in a higher sensitivity. Sample-injection fracture was first applied to match the filmy channel in microchip. Equipped with an amperometric detector, the characteristics of the newly designed filmy channel microchip had been studied and the results showed that it had good reproducibility, higher sensitivity and excellent separation ability. The microchip was also applied to separate L -tryptophan's metabolites, namely 5-hydroxy- L -tryptophan, 5-hydroxytryptamine and 5-hydroxy-indole-3-acetic acid. [source]