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Trifolium Repens (trifolium + repen)
Terms modified by Trifolium Repens Selected AbstractsDirect amplification of nodD from community DNA reveals the genetic diversity of Rhizobium leguminosarum in soilENVIRONMENTAL MICROBIOLOGY, Issue 6 2001Adolphe Zézé Sequences of nodD, a gene found only in rhizobia, were amplified from total community DNA isolated from a pasture soil. The polymerase chain reaction (PCR) primers used, Y5 and Y6, match nodD from Rhizobium leguminosarum biovar trifolii, R. leguminosarum biovar viciae and Sinorhizobium meliloti. The PCR product was cloned and yielded 68 clones that were identified by restriction pattern as derived from biovar trifolii[11 restriction fragment length polymorphism (RFLP) types] and 15 clones identified as viciae (seven RFLP types). These identifications were confirmed by sequencing. There were no clones related to S. meliloti nodD. For comparison, 122 strains were isolated from nodules of white clover (Trifolium repens) growing at the field site, and 134 from nodules on trap plants of T. repens inoculated with the soil. The nodule isolates were of four nodD RFLP types, with 77% being of a single type. All four of these patterns were also found among the clones from soil DNA, and the same type was the most abundant, although it made up only 34% of the trifolii -like clones. We conclude that clover selects specific genotypes from the available soil population, and that R. leguminosarum biovar trifolii was approximately five times more abundant than biovar viciae in this pasture soil, whereas S. meliloti was rare. [source] The influence of arbuscular mycorrhizal colonization and environment on root development in soilEUROPEAN JOURNAL OF SOIL SCIENCE, Issue 4 2003D. Atkinson Summary The production of fine roots is one of the principal means by which carbon, fixed during photosynthesis, enters the soil, and quantifying the production for particular combinations of environmental and biotic factors is important for predicting the sequestration of carbon in the soils of grassland ecosystems. Arbuscular mycorrhizal fungi (AMF) can have a major effect on the production of roots, and we studied how colonization by AMF affects the lifespan of roots. Twenty per cent of control roots of Trifolium repens survived for longer than 42 days whereas 37% survived that long in AMF-colonized plants. The overall survival of the roots of Lolium perenne was less than in T. repens: around 10% of roots survived beyond 42 days and this was not affected by AMF colonization. Previous studies have shown that lifespans of roots can be affected by temperature. We tested the hypothesis that these observations are linked to a change in the morphology of the root system caused by temperature and also by AMF. We found that inoculation with AMF in a microcosm study using Plantago lanceolata grown at various temperatures, with and without AMF, showed no clear effect of AMF on branching patterns. Temperature had a significant effect on total lengths, numbers and branching rates of some higher orders of roots. Total lengths of both secondary and tertiary roots grown at 27°C were about double those of plants grown at 15°C. Colonization by AMF tended to reduce this effect. Evidently the effect of colonization by AMF on root lifespan depends on the species. Increased branching, and thus a greater proportion of ephemeral roots, was responsible for shortening the lives of the roots at increased temperature, which suggests a strong link between lifespan and morphology. [source] Structure and activity of the nitrate-reducing community in the rhizosphere of Lolium perenne and Trifolium repens under long-term elevated atmospheric pCO2FEMS MICROBIOLOGY ECOLOGY, Issue 3 2004Kathrin Deiglmayr Abstract Rhizosphere soil was sampled in monocultures of Lolium perenne and Trifolium repens in June and October 2002, at two different nitrogen fertilisation levels (14 and 56 g N m,2 year,1) and under two pCO2 atmospheres (360 and 600 ppmv) at the Swiss FACE (Free Air Carbon dioxide Enrichment) site. Directly extracted soil DNA was analysed with restriction fragment length polymorphism (PCR-RFLP) by use of degenerated primers for the narG gene encoding the active site of the membrane-bound nitrate reductase. The corresponding enzyme activity of the nitrate reductase was determined colorimetrically after 24 h of anaerobic incubation. The narG PCR-RFLP fingerprints showed that the structure of the nitrate-reducing community was primarily affected by season and pH of the sampling site, whereas CO2 enrichment, plant species or fertiliser treatment had no apparent effect. In contrast, the nitrate reductase activity responded to N fertilisation, CO2 enrichment and plant species in October, whereas in June drought stress most likely kept the enzyme activity at a low level in all treatments. Apparently, the respiratory nitrate-reducing community adapted to different treatments primarily by altered enzyme activity. [source] Seasonal changes in herbage mass and nutritive value of a range of grazed legume swards under Mediterranean and cool temperate conditionsGRASS & FORAGE SCIENCE, Issue 3 2007U. Sölter Summary Seasonal changes in herbage mass and herbage quality of legume-based swards under grazing by sheep or cattle were investigated at four locations in climatically different zones of Europe: Sardinia (Italy), southern France, northern Germany and south-west England (UK). At each location standard treatments were applied to legumes typical of species widely used in each locality: Medicago polymorpha in Italy, Medicago sativa in France, and Trifolium repens in Germany and in UK. At each site comparisons were made of two other legumes: Trifolium subterraneum and Hedysarum coronarium in Italy, Onobrychis sativa and Trifolium incarnatum in France, Trifolium pratense and Lotus corniculatus in Germany, and Trifolium ambiguum and L. corniculatus in UK. Legumes were sown in mixture with locally appropriate companion grasses, and measurements were made over two or three grazing periods. In Italy M. polymorpha swards gave the greatest herbage mass in grazing period 1 but H. coronarium was more persistent. At the French site all legumes established poorly with no significant herbage mass differences between treatments. At both the UK and German sites L. corniculatus maintained a high proportion of legume in the sward; T. repens showed poor persistence under continuous sheep grazing in UK but persisted under cattle grazing in Germany, while T. ambiguum was slow to establish in the UK, and T. pratense proved to be of comparable herbage mass to the standard T. repens -based sward in the last year of the experiment. The concentration of crude protein and in vitro digestibility of organic matter in the dry matter of herbage showed greater within-season variation than between treatments at each site. It is concluded that, in addition to currently used species, legume-based swards containing H. coronarium, O. sativa and L. corniculatus all have potential to contribute to forage production for low-input grazing and their use merits further consideration in systems of livestock production in Europe. [source] Seedling recruitment dynamics of forage and weed species under continuous and rotational sheep grazing in a temperate New Zealand pastureGRASS & FORAGE SCIENCE, Issue 2 2005G. R. Edwards Abstract Understanding the grazing conditions under which plant populations are limited by seed availability (seed limitation) is important for devising management schemes that aim to manipulate the establishment of weed and forage species. Seeds of three weed species (Cirsium arvense, C. vulgare and Rumex obtusifolius) and five forage species (Lolium perenne, Lotus uliginosus syn. L. pedunculatus, Paspalum dilatatum, Plantago lanceolata and Trifolium repens) were broadcast sown into L. perenne,T. repens pastures in Manawatu, New Zealand and five sheep-grazing and two slug-grazing (with and without molluscicide) treatments were imposed in a split-plot design. Of the five sheep-grazing treatments, four compared continuous grazing with rotational grazing at intervals of 12, 24 and 36 d in spring, with all four grazed under a common rotation for the remainder of the year. The fifth treatment was continuous grazing all year. Seed sowing increased seedling emergence of C. vulgare, L. perenne, P. lanceolata, R. obtusifolius and T. repens under all sheep- and slug-grazing treatments, with differences in seedling densities persisting for at least 21 months. Seed sowing did not increase seedling densities of C. arvense, L. uliginosus or P. dilatatum. The effects of sheep-grazing management on seedling emergence and survival were uncoupled. For the five seed-limited species, seedling emergence was greater on pastures that were rotationally grazed during spring compared with those that were continuously grazed. However, seedling survival was lower in pastures grazed rotationally during summer, autumn and winter, so that after 21 months seedling numbers were greater on plots that were continuously grazed all year. Exclusion of slugs increased seedling recruitment of T. repens but had no impact on the other species. As weed and forage species responded in a similar way to sheep-grazing management (increased under continuous, decreased under rotational), it is unlikely that the goals of reducing weed invasions and enhancing forage species establishment could be carried out concurrently in established pastures with the same management. [source] Studying spatial and temporal dynamics of sward structure at low stocking densities: the use of an extended rising-plate-meter methodGRASS & FORAGE SCIENCE, Issue 4 2003O. Correll Abstract An extended rising-plate-meter method was used to study the spatial and temporal variability of the sward structure of extensively managed pastures over a grazing season. Two treatments of a long-term grazing experiment with heifers were investigated: extensive continuous grazing (EG) with a target sward height of 10 cm and intensive continuous grazing (IG) with a target sward height of 5 cm. Compressed sward height and related herbage mass (HM), dominant plant species and stage of development of phenology were determined at weekly or twice weekly intervals at fixed measuring points. The results demonstrated a strong variability in sward height and HM, especially on the EG treatment. The botanical composition of the standing herbage differed between treatments and between patches of different heights within the same treatment. In areas with a short sward, the herbage was predominantly composed of Agrostis capillaris, Festuca rubra and Trifolium repens. It was more evenly composed and also included taller growing species, such as Alopecurus pratensis and Galium mollugo, in areas with a tall sward. The area potentially available for reproductive development was high in the EG treatment and low in the IG treatment. The method employed proved suitable to provide a detailed description of the dynamics of the sward structure. [source] The host plant range of the pea aphid subspecies Acyrthosiphon pisum ssp. destructor (Johnson) (Hom., Aphididae)JOURNAL OF APPLIED ENTOMOLOGY, Issue 6 2002R. I. K. McVean The plant species used were: Lotus uliginosus, Medicago sativa, Melilotus officinalis, Ononis repens, Sarothamnus scoparius, Trifolium hybridum, Trifolium pratense, Trifolium repens, Vicia cracca and Vicia faba. Vicia faba and Trifolium hybridum were the plants on which aphids reached the greatest size, took the least time to reach maturity, and experienced the lowest mortality. The time taken for the aphids to develop to maturity was negatively correlated with adult size, whereas survival to maturity was positively correlated with adult size. The host preference of the aphids was also assessed. The plant species selected as hosts by alatae were those on which their offspring performed best. [source] Uptake of residual phosphate and freshly applied diammonium phosphate by Lolium perenne and Trifolium repens,JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, Issue 5 2003Anne Gallet Abstract Residual fertilizer phosphorus (residual P) may significantly contribute to crop P nutrition. To test this hypothesis, a pot experiment was conducted with ryegrass (Lolium perenne) and clover (Trifolium repens) grown separately on three different soils which either had not received P fertilizer for at least nine years (0F) or had received P fertilizer equivalent to crop P off-take (F). Soils in the pot experiment were given either none (0F, F) or a single rate of 15 mg P (kg soil),1 as diammonium phosphate (0F+DAP, F+DAP). In the treatments 0F+DAP and F+DAP DAP had been labeled with 33PO4 while in the treatments 0F and F the pool of available soil P had been labeled with carrier-free 33PO4. This allowed estimating the quantities of P in plant dry matter that derived from native soil P, residual fertilizer P or fresh fertilizer P. Fourteen to 62,% of the P in the above ground biomass of white clover or perennial ryegrass were derived from residual P whereas 7 to 28,% were derived from freshly applied DAP. The proportion of P derived from residual P was correlated to the total amount of P fertilizer added to the soils, while the proportion of P derived from DAP was correlated to the concentration of P in the soil solution of the 0F and F soils. Aufnahme von Phosphat aus Rückständen von früherer Düngung und aus frisch ausgebrachtem Diammoniumphosphat durch Lolium perenneund Trifolium repens In Düngerrückständen enthaltener Phosphor (P) kann möglicherweise signifikant zur P-Ernährung von Kulturpflanzen beitragen. Um diese Hypothese zu prüfen, wurde im Topfversuch die P-Aufnahme durch Lolium perenne und Trifolium repens untersucht. Als Substrat dienten drei Böden, die entweder seit mindestens 1989 keine P-Gabe mehr erhalten hatten (0F) oder die jährlich P-Gaben im Umfang der P-Entzüge durch die vorgängigen Kulturen erhalten hatten und deshalb P-Rückstände enthielten (F). Es ergaben sich folgende Verfahren: 0F: ohne P aus Düngerrückständen und frischer Düngung; 0F+DAP: mit P aus frischer Düngung (Diammoniumphosphat, 15 mg P (kg Boden),1); F: mit P aus Düngerrückständen; F+DAP: mit P aus Düngerrückständen und frischer Düngung. In den Verfahren 0F + DAP und F + DAP war DAP mit 33PO4 markiert. In den Verfahren 0F und F war der verfügbare P des Bodens mit carrier-freiem 33PO4 markiert. Dies ermöglichte die Erfassung der Aufnahme von P durch die Pflanzen differenziert nach Herkunft aus Boden, Düngerrückständen und frischem Dünger. Von Düngerrückständen stammten 14,62,%, von frischem Dünger 7,28,% des in den Sprossen der Pflanzen gefundenen P. Der von Düngerrückständen stammende Anteil P in den Pflanzensprossen war korreliert mit dem gesamten Gehalt an P das dem Boden als Dünger zugeführt worden war. Der von frischem DAP stammende Anteil war korreliert mit der P-Konzentration in der Bodenlösung. [source] Increased proline loading to phloem and its effects on nitrogen uptake and assimilation in water-stressed white clover (Trifolium repens)NEW PHYTOLOGIST, Issue 3 2009Bok-Rye Lee Summary ,,The aim of this study was to investigate the physiological significance of increased proline loading to phloem caused by water-deficit stress in relation to nitrogen (N) uptake and assimilation. ,,N uptake and N assimilation were quantified by 15N tracing in well-watered (control) and water deficit-stressed white clover (Trifolium repens). De novo proline synthesis and proline loading to the phloem were also compared between treatments. The relationships among proline concentrations in phloem exudates, N uptake, and assimilation of newly absorbed N were assessed. ,,The newly synthesized proline in the phloem exudates increased rapidly after 3 d of water deficit. The water-deficit treatment significantly reduced the maximum nitrate reductase activity (NRA), and also attenuated de novo synthesis of amino acids and proteins in the roots. The increase in proline concentrations in phloem exudates was closely related to reductions in NRA in the roots, N uptake, and the assimilation of newly absorbed N. The accumulation of proline induced in roots by exogenous proline and NH4Cl treatments was closely associated with the decrease in NRA. ,,These results indicate that increased proline transport to roots via phloem caused by water deficit has a significant influence on the down-regulation of N uptake and the assimilation of newly absorbed N. [source] Effects of defoliation intensity on soil food-web properties in an experimental grassland communityOIKOS, Issue 2 2001Juha Mikola We established a greenhouse experiment based on replicated mini-ecosystems to evaluate the effects of defoliation intensity on soil food-web properties in grasslands. Plant communities, composed of white clover (Trifolium repens), perennial ryegrass (Lolium perenne) and plantain (Plantago lanceolata) with well-established root and shoot systems, were subjected to five defoliation intensity treatments: no trimming (defoliation intensity 0, or DI 0), and trimming of all plant material to 35 cm (DI 1), 25 cm (DI 2), 15 cm (DI 3) and 10 cm (DI 4) above soil surface every second week for 14 weeks. Intensification of defoliation reduced shoot production and standing shoot and root mass of plant communities but increased their root to shoot ratio. Soil microbial activity and biomass decreased with intensification of defoliation. Concentrations of NO3,N in soil steadily increased with intensifying defoliation, whereas NH4,N concentrations did not vary between treatments. Numbers of microbi-detritivorous enchytraeids, bacterial-feeding rotifers and bacterial-feeding nematodes steadily increased with intensifying defoliation, while the abundance of fungal-feeding nematodes was significantly enhanced only in DI 3 and DI 4 relative to DI 0. The abundance of herbivorous nematodes per unit soil mass was lower in DI 3 and DI 4 than in DI 0, DI 1 and DI 2, but when calculated per unit root mass, their abundance tended to increase with defoliation intensity. The abundance of omnivorous and predatory nematodes appeared to be highest in the most intensely defoliated systems. The ratio of abundance of fungal-feeding nematodes to that of bacterial-feeding nematodes was not significantly affected by defoliation intensity. The results infer that defoliation intensity may significantly alter the structure of soil food webs in grasslands, and that defoliation per se is able to induce patterns observed in grazing studies in the field. The results did not support hypotheses that defoliation per se would cause a shift between the bacterial-based and fungal-based energy channels in the decomposer food web, or that herbivore and detritivore densities in soil would be highest under intermediate defoliation. Furthermore, our data for microbes and microbial feeders implies that the effects of defoliation intensity on soil food-web structure may depend on the duration of defoliation and are therefore likely to be dynamic rather than constant in nature. [source] Response of Trifolium repens to a mosaic of bare and vegetated patchesPLANT SPECIES BIOLOGY, Issue 1 2000Lizelotte Leeflang Abstract In herbaceous vegetation, plants are subjected to a spatial heterogeneous distribution of light. Trifolium repens was tested on its ability to avoid vegetated patches by change of stolon growth direction, reduced branching or reduced growth. Treatments consisted of a no grass control, grass on the west side and grass on the north side. When Trifolium was growing beside a grass patch on the west side, plants reduced branching but did not change direction nor was there reduced growth in stolons that were growing towards/in the vegetated patch compared to the control plants. When the grass patch was situated to the north of the plant, plants did not show reduced branching except of the primary stolon, nor was there change of growth direction or reduced growth. The biomass and number of ramets were reduced in the vegetated patch of the west treatment and the patch adjacent to the grass patch. In the north treatment biomass and number of ramets were only reduced in the vegetated patch. Compared to the control, total plant biomass was reduced in the west-grass-patch treatment but not in the north-grass-patch treatment. Reduced branching near grass patches could be an added mechanism by which to avoid vegetated patches; it is not very effective, however, because biomass production did not increase in the open patches but was only reduced in the vegetated patches. [source] Revegetation of Pb/Zn Mine Tailings, Guangdong Province, ChinaRESTORATION ECOLOGY, Issue 1 2000Z. H. Ye The Lechang lead/zinc mine is located in the north part of Guangdong Province, southern China. The tailings residue from the extraction of lead/zinc ores was permanently stored in tailings ponds, which required revegetation to reduce the environmental impact. A field study was, therefore, conducted to evaluate the effects of different ameliorants, including: (1) pig manure (PM); (2) mushroom compost (MC); (3) burnt coal residue (BC); (4) fly ash (FA); and (5) surface soil on the growth of Agropyron elongatum (tall wheat grass), Cynodon dactylon (Bermuda grass), Lolium multiflorum (Italian ryegrass), and Trifolium repens (clover) in the tailings residue. The results from the core profiles indicated that adding FA (10 cm) or BC (15 cm) as a barrier layer between the cover soil and the tailings could increase pH, compared to the treatment with soil only. C. dactylon grew well and had a high cover (90,100%) in all the treatment plots except the control plots without any amendment. A. elongatum and L. multiflorum had a higher cover when grown in plots covered with a barrier layer using FA or BC (both with surface soil), than those grown in plots covered with surface soil only. Treatment plots receiving a thicker soil cover (30 cm) had a better dry weight yield than those with a thinner soil cover (15 cm), regardless of the barrier layer. The results from this study indicate that the use of either 15 cm BC or 10 cm FA as a barrier layer with surface soil, or the use of 38 tonnes PM/ha and 6 cm MC, were effective for the revegetation of Pb/Zn mine tailings. C. dactylon was the best species among the four species used for revegetation. Key words: reclamation, Pb/Zn mine tailings, burnt coal, mushroom compost, fly ash, Bermuda grass, Italian ryegrass, clover. [source] Germination and Initial Root Growth of Four Legumes as Affected by Landfill Biogas AtmosphereRESTORATION ECOLOGY, Issue 1 2000L. Marchiol The most important problem in the restoration of closed landfills is the production of toxic gases by decomposition of refuse. Such gases affect the root system of plants growing on these sites. The aim of the present study was to assess the effects induced by landfill biogas contamination on germination and initial root growth of Vicia villosa (hairy vetch), Lotus corniculatus (birdsfoot trefoil), Trifolium pratense (red clover), and Trifolium repens (white clover). In laboratory conditions, simulated landfill and control gas were supplied to the seedlings. The composition of the simulated landfill gas used was: 16% O2, 8% CO2, 3% CH4, and 73% N2; a control gas was also tested (21% O2, 0. 035% CO2, and 78% N2). Percentage of germinated seeds was determined after 6 and 12 days from the starting date; at the same time qualitative assays of metabolic root functionality were also performed by using an agar technique in order to visualize changes in rhizosphere pH. At the end of the experiment, the length of the primary and secondary root was measured. Germination after 6 days was affected by the gas treatment; the landfill biogas caused a delay in germination with respect to the control in seeds of V. villosa and L. corniculatus. Root fresh weight and dry weight were significantly decreased by biogas treatment in V. villosa and T. repens. In contrast, root dry weight was higher in gas treated L. corniculatus and T. pratense compared to control seedlings. Total root system was significantly higher in treated T. pratense. The qualitative assay suggests, with the exception of T. pratense, a metabolic adjustment of the treated seedlings. Key words: restoration, landfill biogas, legumes. [source] Assessing the dominance of Phleum pratense cv. climax, a species commonly used for ski trail restorationAPPLIED VEGETATION SCIENCE, Issue 2 2009Francis Isselin-Nondedeu Abstract Questions: (1) Are some species used for ski trail restoration too dominant to allow native species to re-establish? (2) What plant traits can be used to predict which species are good competitors? We tested the hypothesis that limited native species establishment on ski trails is caused by (1) the dominance of Phleum pratense cv. climax (PPC) and (2) the asymmetry of competitive interactions. Location: Sub-alpine area in the northern French Alps. Methods: PPC was cultivated outdoors over 2 years with 15 alpine species in a systematic design with high- and low-nutrient soil conditions. For each species relative survival, competitive performance and relationships with plant traits were measured. Results: PPC exerted strong dominance on most of its neighbouring species. Survival performance of Anthyllis vulneraria, Luzula sudetica and Lotus alpinus were dramatically reduced. Results of above-ground competition showed that species were trapped in asymmetric competition. Festuca rubra, Trifolium repens, Alchemilla xanthochlora, Trifolium pratense and Plantago alpina best counteracted PPC. Below-ground competition was more symmetric, particularly at the high nutrient level. Plant traits such as biomass, canopy size and specific leaf area were positively correlated with competitive performance of the species. Conclusion: The study has implications for the management of restored ski trails since PPC may hinder the establishment of native sub-alpine species. Consequently, recommendations should focus on (1) maintaining a low proportion or decreasing the proportion of PPC seeds in the revegetation mix and (2) reducing soil fertilization. Plant traits and competition experiments can help to predict changes in restored grasslands. [source] Do virus-resistant plants pose a threat to non-target ecosystems?AUSTRAL ECOLOGY, Issue 5 2009Abstract One key environmental risk associated with the release of novel disease-resistant plants is the potential for non-target host populations to acquire resistance genes and undergo enemy release, leading to damage to associated native plant populations in high conservation-value ecosystems. Unfortunately, the dynamics of most natural pathosystems are poorly understood, and risk assessment of disease-resistant plants remains a challenge. Here we describe the first stage of a multi-tiered risk assessment strategy aimed at quantifying potential ecological release in a model pathosystem (the weedy pasture species Trifolium repens infected with Clover yellow vein virus; ClYVV) in order to assess the level of risk posed by genetically modified and conventionally bred disease-resistant host genotypes to non-target plant communities in south-eastern Australia. Glasshouse inoculation and growth experiments using 14 ClYVV isolates and 20 wild T. repens lines collected from high conservation-value montane grassland and woodland communities show that viral infection reduces the survival and growth of host plants by on average 10,50%. However, T. repens lines exhibited variable levels of resistance and tolerance to virus infection and ClYVV isolates differed in infectivity and aggressiveness, with grassland isolates having a greater pathogenic effect on associated host plants than woodland isolates. We conclude that ClYVV potentially plays an important role in limiting the size of T. repens populations in some at-risk non-target ecosystems and that second-tier field experiments are required to adequately quantify the risk associated with the commercial release of V-R T. repens genotypes in Australia. [source] Do virus-resistant plants pose a threat to non-target ecosystems?AUSTRAL ECOLOGY, Issue 5 2009Abstract It has been widely argued that the acquisition of novel disease resistance genes by wild host populations following the release of novel pathogen-resistant plants into agricultural systems could pose a significant threat to non-target plant communities. However, predicting the magnitude of ecological release in wild plant populations following the removal of disease remains a major challenge. In this paper we report on the second phase of a tiered risk assessment designed to investigate the role of disease on host growth, survival, fecundity and fitness in a model pathosystem (the pasture species Trifolium repens infected with Clover yellow vein virus, ClYVV) and to assess the level of risk posed to at-risk native plant communities in southeast Australia by newly developed genetically modified and conventionally bred virus-resistant T. repens genotypes. Multi-year field experiments conducted in woodland and grassland environments using host-pathogen arrays derived from 14 ClYVV isolates and 21 T. repens genotypes indicate that viral infection reduces fecundity, growth and survival of wild T. repens plants but that the severity of these effects depends on host tolerance to infection, isolate aggressiveness and specific spatial and temporal environmental conditions. Demographic modelling showed that by reducing host survival and growth, ClYVV also limits the intrinsic population growth rate and niche size of wild T. repens populations. Given the significant fitness cost associated with viral infection we conclude that virus-resistant T. repens genotypes may pose a threat to some high conservation-value non-target ecosystems in SE Australia. We also argue that long-term, multi-tiered experiments conducted in a range of controlled and non-controlled environments are necessary to detect and accurately quantify risks associated with the release of disease-resistant plants in general. [source] | |||||||||||||||||||||||||