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Tree Shrews (tree + shrew)
Selected AbstractsImmunohistochemical Localization of Inhibin and Steroidogenic Enzymes in the Ovary of Common Tree Shrew (Tupaia glis) and Northern Smooth-tailed Tree Shrew (Dendrogale murina)ANATOMIA, HISTOLOGIA, EMBRYOLOGIA, Issue 5 2000J. Kimura Summary To study the ovarian function of the Order Scandentia, the localization of inhibin and steroidogenic enzymes (3,-hydroxysteroid dehydrogenase/isomerase and aromatase) in the ovaries of common tree shrew (Tupaia glis) and northern smooth-tailed tree shrew (Dendrogale murina) was immunohistochemically analysed. As in the results reported for other mammals, inhibin ,-chain was localized in the follicular epithelium of secondary or Graafian follicles in the two species. The localization of aromatase in the ovary of these two species, however, was different. In the common tree shrew, the aromatase was localized in the thecal cells, whilst in other mammals it is localized in the granulosa cells. These results indicate that in the ovary of the common tree shrew, the oestradiol may be synthesized in the thecal cells. [source] Organization of the main olfactory bulbs of some mammals: Musk shrews, moles, hedgehogs, tree shrews, bats, mice, and ratsTHE JOURNAL OF COMPARATIVE NEUROLOGY, Issue 1 2004Katsuko Kosaka Abstract We immunohistochemically examined the organization of the main olfactory bulbs (MOBs) in seven mammalian species, including moles, hedgehogs, tree shrews, bats, and mice as well as laboratory musk shrews and rats. We focused our investigation on two points: 1) whether nidi, particular spheroidal synaptic regions subjacent to glomeruli, which we previously reported for the laboratory musk shrew MOBs, are also present in other animals and 2) whether the compartmental organization of glomeruli and two types of periglomerular cells we proposed for the rat MOBs are general in other animals. The general laminar pattern was similar among these seven species, but discrete nidi and the nidal layer were recognized only in two insectivores, namely, the mole and laboratory musk shrew. Olfactory marker protein-immunoreactive (OMP-IR) axons extended beyond the limits of the glomerular layer (GL) into the superficial region of the external plexiform layer (EPL) or the nidal layer in the laboratory musk shrew, mole, hedgehog, and tree shrew but not in bat, mouse, and rat. We observed, in nidi and the nidal layer in the mole and laboratory musk shrew MOBs, only a few OMP-IR axons. In the hedgehog, another insectivore, OMP-IR processes extending from the glomeruli were scattered and intermingled with calbindin D28k-IR cells at the border between the GL and the EPL. In the superficial region of the EPL of the tree shrew MOBs, there were a small number of tiny glomerulus-like spheroidal structures where OMP-IR axons protruding from glomeruli were intermingled with dendritic branches of surrounding calbindin D28k-IR cells. Furthermore, we recognized the compartmental organization of glomeruli and two types of periglomerular cells in the MOBs of all of the mammals we examined. These structural features are therefore considered to be common and important organizational principles of the MOBs. J. Comp. Neurol. 472:1,12, 2004. © 2004 Wiley-Liss, Inc. [source] Immunohistochemical Localization of Inhibin and Steroidogenic Enzymes in the Ovary of Common Tree Shrew (Tupaia glis) and Northern Smooth-tailed Tree Shrew (Dendrogale murina)ANATOMIA, HISTOLOGIA, EMBRYOLOGIA, Issue 5 2000J. Kimura Summary To study the ovarian function of the Order Scandentia, the localization of inhibin and steroidogenic enzymes (3,-hydroxysteroid dehydrogenase/isomerase and aromatase) in the ovaries of common tree shrew (Tupaia glis) and northern smooth-tailed tree shrew (Dendrogale murina) was immunohistochemically analysed. As in the results reported for other mammals, inhibin ,-chain was localized in the follicular epithelium of secondary or Graafian follicles in the two species. The localization of aromatase in the ovary of these two species, however, was different. In the common tree shrew, the aromatase was localized in the thecal cells, whilst in other mammals it is localized in the granulosa cells. These results indicate that in the ovary of the common tree shrew, the oestradiol may be synthesized in the thecal cells. [source] Long-term biomechanical properties of rabbit sclera after collagen crosslinking using riboflavin and ultraviolet A (UVA)ACTA OPHTHALMOLOGICA, Issue 2 2009Gregor Wollensak Abstract. Purpose:, Scleral crosslinking by the photosensitizer riboflavin and ultraviolet A (UVA) has been shown to increase significantly the scleral biomechanical rigidity and might therefore become a possible sclera-based treatment modality for progressive myopia. In the present study, the long-term effect of the new crosslinking method on biomechanical properties was investigated in the rabbit sclera. Methods:, A 10 × 10 mm sector of the equatorial sclera of nine Chinchilla rabbit eyes was treated in vivo using a UVA double diode of 370 nm with a surface irradiance of 3 mW/cm2 and application of 0.1% riboflavin-5-phosphate drops as photosensitizer for 30 min. Three days, 4 months and 8 months postoperatively, biomechanical stress,strain measurements of the treated scleral strips were performed and compared to contralateral control sclera using a microcomputer-controlled biomaterial tester. In addition, routine histological controls were performed. Results:, Following the crosslinking treatment, Young's modulus was increased by 320% after 3 days, 277% after 4 months and 502% after 8 months, and ultimate stress by 341% after 3 days, 131% after 4 months and 213.8% after 8 months versus the controls. The decrease in ultimate strain was between 24% and 44.8%. On histology, no tissue damage was detected. Conclusion:, Our new method of scleral collagen crosslinking proved very effective and constant over a time interval of up to 8 months in increasing the scleral biomechanical strength. Therefore, the new treatment might become an option for strengthening scleral tissue in progressive myopia and other conditions associated with weakened sclera. There were no side-effects on the retina or retinal pigment epithelium. The new crosslinking treatment could now be tested in a suitable myopia model (like the tree shrew) and finally in human eyes. [source] ,2A and ,2C -adrenoceptor regulation in the brain: ,2A changes persist after chronic stressEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 5 2003G. Flügge Abstract Stress-induced activation of the central nervous noradrenergic system has been suspected to induce depressive disorders. As episodes of depression often occur some time after a stress experience we investigated whether stress-induced changes in the ,2 -adrenoceptor (,2 -AR) system persist throughout a post-stress recovery period. Brains of male tree shrews were analysed after 44 days of chronic psychosocial stress and after a subsequent 10-day recovery period. Expression of RNA for ,2A and ,2C -adrenoceptors was quantified by in situ hybridization, and receptor binding was determined by in vitro receptor autoradiography. Activities of the sympathetic nervous system and of the hypothalamo,pituitary,adrenal axis were increased during chronic stress but normalized during recovery. ,2A -AR RNA in the glutamatergic neurons of the lateral reticular nucleus was elevated significantly after stress and after recovery (by 29% and 17%). In the dorsal motor nucleus of the vagus, subtype A expression was enhanced after recovery (by 33%). In the locus coeruleus, subtype A autoreceptor expression was not changed significantly. Subtype C expression in the caudate nucleus and putamen was elevated by stress (by 5 and 4%, respectively) but normalized during recovery. Quantification of 3H-RX821002 binding revealed receptor upregulation during stress and/or recovery. Our data therefore show: (i) that chronic psychosocial stress differentially regulates expression of ,2 -adrenoceptor subtypes A and C; (ii) that subtype A heteroreceptor expression is persistently upregulated whereas (iii), subtype C upregulation is only transient. The present findings coincide with post mortem studies in depressed patients revealing upregulation of ,2A -ARs. [source] Variation in mammalian proximal femoral development: comparative analysis of two distinct ossification patternsJOURNAL OF ANATOMY, Issue 3 2007Maria A. Serrat Abstract The developmental anatomy of the proximal femur is complex. In some mammals, including humans, the femoral head and greater trochanter emerge as separate ossification centres within a common chondroepiphysis and remain separate throughout ontogeny. In other species, these secondary centres coalesce within the chondroepiphysis to form a single osseous epiphysis much like the proximal humerus. These differences in femoral ontogeny have not been previously addressed, yet are critical to an understanding of femoral mineralization and architecture across a wide range of mammals and may have key implications for understanding and treating hip abnormalities in humans. We evaluated femora from 70 mammalian species and categorized each according to the presence of a ,separate' or ,coalesced' proximal epiphysis based on visual assessment. We found that ossification type varies widely among mammals: taxa in the ,coalesced' group include marsupials, artiodactyls, perissodactyls, bats, carnivores and several primates, while the ,separate' group includes hominoids, many rodents, tree shrews and several marine species. There was no clear relationship to body size, phylogeny or locomotion, but qualitative and quantitative differences between the groups suggest that ossification type may be primarily an artefact of femoral shape and neck length. As some osseous abnormalities of the human hip appear to mimic the normal morphology of species with coalesced epiphyses, these results may provide insight into the aetiology and treatment of human hip disorders such as femoroacetabular impingement and early-onset osteoarthritis. [source] Conserved cellular function and stress-mediated regulation among members of the proteolipid protein familyJOURNAL OF NEUROSCIENCE RESEARCH, Issue 6 2010María E. Fernández Abstract Chronic stress causes morphological alterations in the hippocampus of rodents and tree shrews, including atrophy of CA3 dendrites and loss of synapses. The molecular mechanisms underlying these structural changes remain largely unknown. We have previously identified M6a as a stress responsive gene and shown that M6a is involved in filopodium/spine outgrowth and, likely, synapse formation. M6a belongs to the proteolipid protein (PLP) family, all of their members having four transmembrane domains that allow their localization at the plasma membrane. In the present work, we analyzed other members of this family, the closely related M6b as well as PLP and its splice variant DM20. We found that chronic restraint stress in mice reduces M6b and DM20, but not PLP, mRNA levels in the hippocampus. In addition, M6b and DM20, but again not PLP, induce filopodium formation in primary cultures of hippocampal neurons. Several M6b protein isoforms were studied, all of them having similar effects except for the one lacking the transmembrane domains. Our results reveal a conserved cellular function and a stress-mediated regulation among members of the proteolipid protein family, suggesting an involvement of proteolipid proteins in the stress response. © 2009 Wiley-Liss, Inc. [source] Frontal Fusion: Collapse of Another Anthropoid SynapomorphyTHE ANATOMICAL RECORD : ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY, Issue 3 2008Alfred L. Rosenberger Abstract We test the hypothesis that the fused interfrontal suture of anthropoids is a uniquely distinguishing feature and a derived characteristic indicative of their monophyletic origin. Our survey of nonanthropoid primates and several archontan families indicates frontal fusion is widespread. It is most variable (fused, open or partially fused) inter- and intra-specifically among strepsirhines. The frontal bone is more commonly fused in living lemuroids and indrioids than among lorisoids. It appears to be fused regularly among Eocene adapids. Among nonanthropoid haplorhines, the interfrontal is fused in Tarsius, even in neonates and invariably in adults, probably also in all fossil tarsiiforms preserving the frontal bone, and in the late Eocene protoanthropoid Rooneyia. The plesiadapiform pattern remains uncertain, but fusion is ubiquitous among living tree shrews, colugos and bats. Distributional evidence implies that interfrontal fusion was present in the last common ancestor (LCA) of haplorhine primates and possibly in the LCA of euprimates as well. Anthropoids, therefore, cannot be defined cladistically by interfrontal fusion, not out of concern for homoplasy but because it is probably a primitive feature inherited from other taxa related to anthropoids. Fusion of the large anthropoid frontal bone, which was extended anteriorly to roof the orbits and expanded laterally in connection with a wide forebrain in the LCA of anthropoids and protoanthropoids, may have been preadaptive to the evolution of the postorbital septum. The zygomatico-frontal suture of the septum may provide an alternative mechanism for dissipating the calvarial strains of mastication formerly taken up by an open interfrontal suture. Anat Rec, 291:308,317, 2008. © 2008 Wiley-Liss, Inc. [source] Organization of the main olfactory bulbs of some mammals: Musk shrews, moles, hedgehogs, tree shrews, bats, mice, and ratsTHE JOURNAL OF COMPARATIVE NEUROLOGY, Issue 1 2004Katsuko Kosaka Abstract We immunohistochemically examined the organization of the main olfactory bulbs (MOBs) in seven mammalian species, including moles, hedgehogs, tree shrews, bats, and mice as well as laboratory musk shrews and rats. We focused our investigation on two points: 1) whether nidi, particular spheroidal synaptic regions subjacent to glomeruli, which we previously reported for the laboratory musk shrew MOBs, are also present in other animals and 2) whether the compartmental organization of glomeruli and two types of periglomerular cells we proposed for the rat MOBs are general in other animals. The general laminar pattern was similar among these seven species, but discrete nidi and the nidal layer were recognized only in two insectivores, namely, the mole and laboratory musk shrew. Olfactory marker protein-immunoreactive (OMP-IR) axons extended beyond the limits of the glomerular layer (GL) into the superficial region of the external plexiform layer (EPL) or the nidal layer in the laboratory musk shrew, mole, hedgehog, and tree shrew but not in bat, mouse, and rat. We observed, in nidi and the nidal layer in the mole and laboratory musk shrew MOBs, only a few OMP-IR axons. In the hedgehog, another insectivore, OMP-IR processes extending from the glomeruli were scattered and intermingled with calbindin D28k-IR cells at the border between the GL and the EPL. In the superficial region of the EPL of the tree shrew MOBs, there were a small number of tiny glomerulus-like spheroidal structures where OMP-IR axons protruding from glomeruli were intermingled with dendritic branches of surrounding calbindin D28k-IR cells. Furthermore, we recognized the compartmental organization of glomeruli and two types of periglomerular cells in the MOBs of all of the mammals we examined. These structural features are therefore considered to be common and important organizational principles of the MOBs. J. Comp. Neurol. 472:1,12, 2004. © 2004 Wiley-Liss, Inc. [source] | ||||||||||||||||||||||