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Total Protein Concentration (total + protein_concentration)
Selected AbstractsLevels of pre-kallikrein in resting and stimulated human parotid and submandibular salivaEUROPEAN JOURNAL OF ORAL SCIENCES, Issue 5 2001Carol A. Francis Salivary tissue kallikrein is stored in an active form in human salivary glands. Pre-kallikrein has been demonstrated in mixed saliva, but it is not clear if the various salivary glands contribute equally. This study set out to determine if pre-kallikrein is present in human parotid and submandibular salivas at rest, whether levels change during stimulation, and to compare the pattern of pre-kallikrein and kallikrein secretion with that of total protein. Resting and citric acid-stimulated parotid and submandibular, and gum-stimulated parotid saliva samples were collected from 6 healthy subjects. Salivary flows were determined gravimetrically. Total protein concentration and kallikrein enzymic activity were assayed using standard techniques. Pre-kallikrein was assayed following trypsinisation of duplicate samples. Pre-kallikrein was present in parotid and submandibular ductal saliva. Proportions of pre-kallikrein and active kallikrein were similar in salivas secreted at rest and during stimulation, and both outputs mirrored protein output in both major glands. Gum-stimulated parotid saliva showed lower activity than resting, and no differences were seen between resting and stimulated submandibular samples. [source] CSF analysis in patients with sporadic CJD and other transmissible spongiform encephalopathiesEUROPEAN JOURNAL OF NEUROLOGY, Issue 2 2007A. Green Patients with suspected Creutzfeldt,Jakob disease (CJD) often have routine cerebrospinal fluid (CSF) analysis performed to exclude treatable inflammatory conditions; however, little information is available about the range of results obtained for CSF tests in patients with sporadic CJD and other transmissible spongiform encephalopathies (TSE). Data from 450 patients with sporadic CJD and 47 patients with other TSEs were collected as part of an EC-supported multinational study. Raised white cell counts of >5 cells/,l were found in three of 298 patients with sporadic CJD, with two cell counts of 7 cells/,l and one of 20 cells/,l. Total protein concentrations of >0.9 g/l were found in five of 438 patients with sporadic CJD, although none had a concentration of >1 g/l. CSF oligoclonal IgG was detected in eight of 182 sporadic CJD patients. Of the patients with other TSEs, six had elevated cell counts ranging from 6 to 14 cells/,l but none had total protein concentrations of >0.9 g/l and one patient had detectable oligoclonal IgG. None of the patients with sporadic CJD or other TSEs had abnormalities in all three tests. [source] Effect of Age and Abomasal Puncture on Peritoneal Fluid, Hematology, and Serum Biochemical Analyses in Young CalvesJOURNAL OF VETERINARY INTERNAL MEDICINE, Issue 6 2005Luiz Claudio N. Mendes The goals of this study were to evaluate techniques for collection of peritoneal fluid from calves, establish reference ranges for fibrinogen in peritoneal fluid during the 1st month of life, and determine if abomasal puncture would alter peritoneal fluid or hematologic variables. Twenty-two healthy Holstein calves underwent 3 peritoneal fluid collections on day 1, day 15, and day 30 of age. Fibrinogen concentration in peritoneal fluid was 0.20 g/dL and 0.10 g/dL (P < .05) for day 1 and day 30, respectively, and 0.10 at day 15 (P > .05) for calves without abomasal puncture. Plasma fibrinogen concentration was 0.60 g/dL and 0.70 g/dL (P < .05) for days 15 and 30, respectively, in calves without abomasal puncture. There were no significant differences (P, .05) in peritoneal fluid and peripheral blood total protein and fibrinogen concentrations, specific gravity, total and differential cell count, or erythrocyte counts between calves with or without abomasal puncture. We concluded that the reference ranges established for fibrinogen and total protein concentration are important for accurate evaluation of peritoneal fluid in calves for further comparison with similar-aged animals with gastrointestinal-tract or abdominal-cavity disease. Additionally, accidental abomasal puncture does not alter values of fibrinogen, total protein, and nucleated cell count in peritoneal fluid and does not cause apparent clinical abnormalities. [source] Penetration of ceftiofur into sterile vs.JOURNAL OF VETERINARY PHARMACOLOGY & THERAPEUTICS, Issue 3 2005Mannheimia haemolytica -infected tissue chambers in beef calves after subcutaneous administration of ceftiofur crystalline free acid sterile suspension in the ear pinna The effect of Mannheimia haemolytica infection on the penetration of ceftiofur and desfuroylceftiofur metabolites into tissue chambers was studied in cattle after subcutaneous administration of ceftiofur crystalline free acid sterile suspension (CCFA-SS). Four tissue chambers were implanted subcutaneously in each of 12 calves. Approximately 45 days after implantation, two chambers were inoculated with M. haemolytica (106 colony-forming units per chamber) while the remaining two chambers were inoculated with sterile phosphate-buffered saline. Twenty-four hours after inoculation, CCFA-SS was administered subcutaneously in the middle third of the caudal ear pinna of each calf. Chamber fluid and blood samples were collected at predetermined times for 10 days following dosing and analyzed for ceftiofur and desfuroylceftiofur metabolites by high-performance liquid chromatography. Concentrations of ceftiofur and desfuroylceftiofur metabolites in plasma and tissue chamber fluid remained above a threshold of 0.2 ,g/mL for at least 8 days. Infected tissue chamber fluid concentrations of ceftiofur and desfuroylceftiofur metabolites were significantly higher than those in non-infected tissue chamber fluid, which correlated with significantly higher total protein concentration in infected tissue chambers. These results indicate that single subcutaneous administration of CCFA-SS at 6.6 mg/kg can be expected to provide effective therapy of susceptible bacterial infections for a period of at least 1 week. [source] Chronic inflammatory demyelinating polyneuropathy associated with tumor necrosis factor-, antagonistsMUSCLE AND NERVE, Issue 5 2010Amer Alshekhlee MD Abstract Biologic therapy with tumor necrosis factor (TNF)-, antagonists for rheumatoid arthritis has been well established. We describe two patients with rheumatoid arthritis who developed chronic inflammatory demyelinating polyneuropathy (CIDP) during their course of therapy with TNF-, antagonists. A 45-year-old woman and a 49-year-old man, both with a history of rheumatoid arthritis, were treated with etanercept and infliximab, respectively. Clinical signs of peripheral neuropathy developed 2 weeks and 12 months after the initiation of TNF-, antagonists. Electrodiagnostic studies at variable points during the disease course showed signs of acquired demyelination consistent with CIDP. Cerebrospinal fluid examination showed albuminocytologic dissociation (total protein concentration 118 mg/dl and 152 mg/dl, respectively). Both patients failed to improve after discontinuation of the offending agent, and they responded poorly to corticosteroids. However, there was clinical and electrophysiologic recovery after initiation of intravenous immunoglobulin (IVIg) therapy. CIDP may occur early or late during the treatment course with TNF-, antagonists. IVIg may reverse and stabilize the inflammatory process. Muscle Nerve 41: 742,747, 2010 [source] C-C chemokine receptor 2 (CCR2) deficiency improves bleomycin-induced pulmonary fibrosis by attenuation of both macrophage infiltration and production of macrophage-derived matrix metalloproteinasesTHE JOURNAL OF PATHOLOGY, Issue 5 2004Toshiyuki Okuma Abstract Macrophage infiltration is implicated in various types of pulmonary fibrosis. One important pathogenetic process associated with pulmonary fibrosis is injury to basement membranes by matrix metalloproteinases (MMPs) that are produced mainly by macrophages. In this study, C-C chemokine receptor 2-deficient (CCR2,/,) mice were used to explore the relationship between macrophage infiltration and MMP activity in the pathogenesis of pulmonary fibrosis, using the bleomycin-induced model of this disease process. CCR2 is the main (if not only) receptor for monocyte chemoattractant protein-1/C-C chemokine ligand 2 (MCP-1/CCL2), which is a critical mediator of macrophage trafficking, and CCR2 ,/, mice demonstrate defective macrophage migration. Pulmonary fibrosis was induced in CCR2,/, and wild-type (CCR2+/+) mice by intratracheal instillation of bleomycin. No significant differences in the total protein concentration in bronchoalveolar lavage (BAL) fluid, or in the degree of histological lung inflammation, were observed in the two groups until day 7. Between days 3 and 21, however, BAL fluid from CCR2,/, mice contained fewer macrophages than BAL fluid from CCR2+/+ mice. Gelatin zymography of BAL fluid and in situ zymography revealed reduced gelatinolytic activity in CCR2,/, mice. Immunocytochemical staining showed weaker expression of MMP-2 and MMP-9 in macrophages in BAL fluid from CCR2,/, mice at day 3. Gelatin zymography of protein extracted from alveolar macrophages showed reduced gelatinolytic activity of MMP-2 and MMP-9 in CCR2,/, mice. At days 14 and 21, lung remodelling and the hydroxyproline content of lung tissues were significantly reduced in CCR2,/, mice. These results suggest that the CCL2/CCR2 functional pathway is involved in the pathogenesis of bleomycin-induced pulmonary fibrosis and that CCR2 deficiency may improve the outcome of this disease by regulating macrophage infiltration and macrophage-derived MMP-2 and MMP-9 production. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. [source] An analysis of refractometry as a method of determining blood total protein concentration in the American lobster Homarus americanus (Milne Edwards)AQUACULTURE RESEARCH, Issue 8 2002G Ozbay Abstract Given the high unit value of live American lobsters (Homarus americanus), a non-destructive field method to assess physiological state is desirable during out-of-water transport conditions. This study compared an Uricon® specific gravity refractometer, with a commercial veterinary blood analyser (Vet-Test), as a method for determining the blood total protein concentration in live, out-of-water individuals. Regression analysis of R2 = 0.8641 (n = 64) was observed in determination of blood total protein concentration, y = 0.0036x + 1.0163 where x is the protein concentration in mg dL,1 in Vet-Test. Our results (R2 = 0.864, SE ± 0.003) support the results (R2 = 0.985) of the previous study conducted by Leavitt & Bayer (1977) in which serum total protein was compared with the refractometric method. We therefore confirm that the refractometric method can be used as a reliable method to indicate health of a lobster by measuring the blood total protein concentration. The results show good correlation between the protein concentrations observed using the refractometric method and a Vet-Test blood chemistry analyser. anova analysis was significant between the protein refractometer and the Vet-Test (P < 0.05). Although protein concentration units obtained from both methods were different the results followed the same trends. Refractometry as a method is reliable to determine blood total protein concentrations in the American lobster. Consequently, a refractometric method can be used directly by commercial growers and distributors to assess responses to holding facility conditions and to feeding regimes. Whole blood protein concentrations may provide more information than serum protein concentrations as measured in the study of Leavitt & Bayer (1977). [source] High Recovery Refolding of rhG-CSF from Escherichia coli, Using Urea Gradient Size Exclusion ChromatographyBIOTECHNOLOGY PROGRESS, Issue 1 2008Chaozhan Wang Protein folding liquid chromatography (PFLC) is a powerful tool for simultaneous refolding and purification of recombinant proteins in inclusion bodies. Urea gradient size exclusion chromatography (SEC) is a recently developed protein refolding method based on the SEC refolding principle. In the presented work, recombinant human granulocyte colony-stimulating factor (rhG-CSF) expressed in Escheriachia coli ( E. coli) in the form of inclusion bodies was refolded with high yields by this method. Denatured/reduced rhG-CSF in 8.0 mol·L -1 urea was directly injected into a Superdex 75 column, and with the running of the linear urea concentration program, urea concentration in the mobile phase and around the denatured rhG-CSF molecules was decreased linearly, and the denatured rhG-CSF was gradually refolded into its native state. Aggregates were greatly suppressed and rhG-CSF was also partially purified during the refolding process. Effects of the length and the final urea concentration of the urea gradient on the refolding yield of rhG-CSF by using urea gradient SEC were investigated respectively. Compared with dilution refolding and normal SEC with a fixed urea concentration in the mobile phase, urea gradient SEC was more efficient for rhG-CSF refolding&‐;in terms of specific bioactivity and mass recovery, the denatured rhG-CSF could be refolded at a larger loading volume, and the aggregates could be suppressed more efficiently. When 500 ,L of solubilized and denatured rhG-CSF in 8.0 mol·L -1 urea solution with a total protein concentration of 2.3 mg·mL -1 was loaded onto the SEC column, rhG-CSF with a specific bioactivity of 1.0 × 108 IU·mg -1 was obtained, and the mass recovery was 46.1%. [source] Ocular penetration of intravenously administered enrofloxacin in the horseEQUINE VETERINARY JOURNAL, Issue 2 2008T. J. DIVERS Summary Reason for performing study: Information on antibiotic concentrations in the equine eye following systemic therapy is limited. Reports that Leptospira spp. are frequently present in the eyes of horses with recurrent uveitis, emphasises a need for studies on ocular concentrations of specific antibiotics. Hypotheses: 1) Enrofloxacin, administered i.v. at 7.5 mg/kg bwt q. 24 h, results in aqueous humour concentrations greater than the reported minimum inhibitory concentration (MIC) for Leptospira pomona. 2) Aqueous humour paracentesis sufficiently disrupts the blood-aqueous humour barrier (BAB) to cause an increase in aqueous humour protein and enrofloxacin concentrations. Methods: Aqueous humour enrofloxacin and total protein concentrations were determined in 6 healthy, mature horses after i.v. administration of enrofloxacin. Paracentesis was performed on the left eye on Days 3 and 4, one hour following enrofloxacin administration, to determine enrofloxacin concentrations in healthy eyes and in eyes with mechanical disruption of the BAB. Paracentesis was also performed on the right eye 23 h after enrofloxacin administration. Blood samples were collected from the horses at identical times to determine enrofloxacin aqueous humour:plasma ratios. Results: Mean ± s.d. enrofloxacin concentration in the aqueous humour one hour post administration on Day 3 was 0.32 ± 0.10 mg/l (range 0.18-0.47); and aqueous humour enrofloxacin, total protein and aqueous humour:plasma enrofloxacin ratios were higher on Day 4 than Day 3. Conclusions and potential relevance: Following disruption of the BAB, enrofloxacin concentrations were above the reported MIC for Leptospira pomona. [source] CSF analysis in patients with sporadic CJD and other transmissible spongiform encephalopathiesEUROPEAN JOURNAL OF NEUROLOGY, Issue 2 2007A. Green Patients with suspected Creutzfeldt,Jakob disease (CJD) often have routine cerebrospinal fluid (CSF) analysis performed to exclude treatable inflammatory conditions; however, little information is available about the range of results obtained for CSF tests in patients with sporadic CJD and other transmissible spongiform encephalopathies (TSE). Data from 450 patients with sporadic CJD and 47 patients with other TSEs were collected as part of an EC-supported multinational study. Raised white cell counts of >5 cells/,l were found in three of 298 patients with sporadic CJD, with two cell counts of 7 cells/,l and one of 20 cells/,l. Total protein concentrations of >0.9 g/l were found in five of 438 patients with sporadic CJD, although none had a concentration of >1 g/l. CSF oligoclonal IgG was detected in eight of 182 sporadic CJD patients. Of the patients with other TSEs, six had elevated cell counts ranging from 6 to 14 cells/,l but none had total protein concentrations of >0.9 g/l and one patient had detectable oligoclonal IgG. None of the patients with sporadic CJD or other TSEs had abnormalities in all three tests. [source] Stimulated whole salivary flow rate and composition in menopausal women with oral dryness feelingORAL DISEASES, Issue 3 2007F Agha-Hosseini The aim of this study was to compare stimulated whole saliva flow rate and composition of menopausal women with/without oral dryness (OD) feeling. A case,control study was carried out in 42 selected menopausal women aged 52,73 years with or without OD feeling (21 as case and 21 as control) conducted at the Clinic of Oral Medicine, Tehran University of Medical Sciences. Paraffin-stimulated saliva samples were obtained by expectoration. The stimulated whole saliva composition was measured by a spectrophotometer [magnesium (Mg+2), calcium (Ca+2), chloride (Cl,), inorganic phosphate (Pi) and total protein], flame-photometry [sodium (Na+)] and ion selective electrode (ISE) [potassium (K+)] methods. No significant differences were found in stimulated whole saliva flow rate, Mg+2, Cl,, Pi, Na+, K+ and total protein concentrations between the two groups, but the mean calcium concentration was significantly higher in cases than in controls (P = 0.003). It seems that the level of salivary calcium concentration may be higher in menopausal women with OD feeling than in the control group. [source] Quantitative dietary threonine requirement of juvenile Pacific white shrimp, Litopenaeus vannamei (Boone) reared in low-salinity waterAQUACULTURE RESEARCH, Issue 8 2009Ming-Yan Huai Abstract An 8-week feeding trial was conducted to determine the threonine requirement of juvenile Pacific white shrimp Litopenaeus vannamei (Boone) in low-salinity water (0.50,1.50 g L,1). Diets 1,6 were formulated to contain 360 g kg,1 crude protein with fish meal, wheat gluten and pre-coated crystalline amino acids with six graded levels of l -threonine (9.9,19.0 g kg,1 dry diet). Diet 7, which was served as a reference, contained only intact proteins (fish meal and wheat gluten). Each diet was randomly assigned to triplicate groups of 30 shrimps (0.48±0.01 g), each four times daily. Shrimps fed the reference diet had similar growth performance and feed utilization efficiency compared with shrimps fed the diets containing 13.3 g kg,1 or higher threonine. Maximum specific growth rate (SGR) and protein efficiency ratio were obtained at 14.6 g kg,1 dietary threonine, and increasing threonine beyond this level did not result in a better performance. Body compositions, triacyglycerol and total protein concentrations in haemolymph were significantly affected by the threonine level; however, the threonine contents in muscle, aspartate aminotransferase and alanine aminotransferase activities in haemolymph were not influenced by the dietary threonine levels. Broken-line regression analysis on SGR indicated that optimal dietary threonine requirement for L. vannamei was 13.6 g kg,1 dry diet (37.8 g kg,1 dietary protein). [source] An analysis of refractometry as a method of determining blood total protein concentration in the American lobster Homarus americanus (Milne Edwards)AQUACULTURE RESEARCH, Issue 8 2002G Ozbay Abstract Given the high unit value of live American lobsters (Homarus americanus), a non-destructive field method to assess physiological state is desirable during out-of-water transport conditions. This study compared an Uricon® specific gravity refractometer, with a commercial veterinary blood analyser (Vet-Test), as a method for determining the blood total protein concentration in live, out-of-water individuals. Regression analysis of R2 = 0.8641 (n = 64) was observed in determination of blood total protein concentration, y = 0.0036x + 1.0163 where x is the protein concentration in mg dL,1 in Vet-Test. Our results (R2 = 0.864, SE ± 0.003) support the results (R2 = 0.985) of the previous study conducted by Leavitt & Bayer (1977) in which serum total protein was compared with the refractometric method. We therefore confirm that the refractometric method can be used as a reliable method to indicate health of a lobster by measuring the blood total protein concentration. The results show good correlation between the protein concentrations observed using the refractometric method and a Vet-Test blood chemistry analyser. anova analysis was significant between the protein refractometer and the Vet-Test (P < 0.05). Although protein concentration units obtained from both methods were different the results followed the same trends. Refractometry as a method is reliable to determine blood total protein concentrations in the American lobster. Consequently, a refractometric method can be used directly by commercial growers and distributors to assess responses to holding facility conditions and to feeding regimes. Whole blood protein concentrations may provide more information than serum protein concentrations as measured in the study of Leavitt & Bayer (1977). [source] Comparison of cysteinyl leukotriene concentrations between exhaled breath condensate and bronchoalveolar lavage fluidCLINICAL & EXPERIMENTAL ALLERGY, Issue 12 2008E. Ono Summary Background Collection of exhaled breath condensate (EBC) is a simple, non-invasive method of obtaining samples from the airways and it can be repeated in short intervals without side effects; therefore, it provides an opportunity to monitor the changes in concentration of inflammatory mediators in the airways. However, EBC analysis still has several unresolved issues. Objective To better understand the characteristics of EBC, we compared cysteinyl leukotriene (CysLT) concentrations between bronchoalveolar lavage fluid (BALF) and EBC. We also attempted to correct CysLT concentrations in BALF and EBC diluted with saline and water vapour using biological markers. Methods EBC was collected from 14 patients with idiopathic pulmonary fibrosis before bronchoscopy. We measured CysLT concentrations and also quantified tyrosine, urea and total protein as possible biomarkers for correcting dilution. Results (1) We have validated the quantification of CysLTs in EBC. (2) Although a significant correlation was observed among tyrosine and urea concentrations in BALF, urea and total protein concentrations were below the detection limit in EBC. (3) CysLT concentrations were higher in BALF than in EBC (median, 15.96 pg/mL vs. 5.5 pg/mL; P=0.001) and there was no correlation of CysLT concentrations in BALF with those in EBC. A significant correlation of the ratio of total CysLT concentration to tyrosine concentration (CysLT/Y) in EBC with that in BALF was observed (r=0.547, P=0.043). (4) CysLT/Y in EBC correlated with serum KL-6 concentration and total cell count in BALF, and CysLT/Y in BALF also correlated with exhaled NO concentration and %VC. Conclusions CysLT/Y in EBC significantly correlated with that in BALF and some clinical parameters correlated with CysLT/Y. Tyrosine concentration may be used to correct the dilution error for CysLT concentrations, and CysLT/Y in EBC can be a surrogate marker for CysLT concentrations in BALF. [source] | ||||||||||||||||||||||