Basic Technologies (basic + technology)

Distribution by Scientific Domains

Selected Abstracts

Cover Picture: Electrophoresis 18'2010

Article first published online: 20 SEP 2010
Issue no. 18 is a special issue on "Miniaturization in Asia-Pacific" comprising 21 manuscripts distributed over 5 separate parts. Part I has 1 review article on microfluidics gradient platform for controlling cellular behavior. Part II has 6 research articles dealing with various basic technologies in microfluidics. Part III reports a variety of detection approaches described in 6 research articles. DNA and protein analysis using microfluidics are treated in 3 research articles making up part IV. The last 5 articles in this issue (Part V) are on cell analysis. [source]

Cover Picture: Electrophoresis 18'2009

Article first published online: 17 SEP 200
Issue no. 18 is a special issue on "Miniaturization in Asia-Pacific". It has 25 contributions "covering a spectrum of topics pertaining to fundamentals, basic technologies, detection approaches and applications, and it is expected to reflect the rapid progress of up-to-date microfluidics research in the Asia-Pacific region." [source]

Cover Picture: Electrophoresis 9/2008

Article first published online: 28 APR 200
Regular issues provide a wide range of research and review articles covering all aspects of electrophoresis. Here you will find cutting-edge articles on methods and theory, instrumentation, nucleic acids, CE and CEC, miniaturization and microfluidics, proteomics and two-dimensional electrophoresis. The 20 papers gathered here span areas across miniaturization theory, basic technologies and applications. While not comprehensive, this special issue represents a selection of the state-of-the-art science of miniaturization and its application in various areas of life sciences. We feel to a large degree that this special issue sets a good foundation, and we are looking forward to another thought-provoking update in the near future. Issue 9 also offers one Fast Track article describing particularly important investigations in electrophoresis: "AC Electrokinetic separation and detection of DNA nanoparticles in high conductance solutions." [source]

Establishment of a chick embryo model for analyzing liver development and a search for candidate genes

Yuji Yokouchi
The liver plays a crucial role in metabolism. There is considerable interest in how the liver develops, as such knowledge could prove of importance in regenerative medicine. However, our understanding of liver development remains somewhat limited. We have developed a model system using the chick embryo that is cost effective and is easy to manipulate experimentally. We performed four fundamental studies: (i) construction of an atlas of the developing chick liver; (ii) identification of differentiation marker genes in the developing chick embryo; (iii) development of germ-layer specific electroporation; and (iv) establishment of organ culture from the developing chick liver. Using this system, we have been able to demonstrate the functions of candidate genes within a shorter period and in a more cost-effective manner. In parallel with the establishment of this system, we examined the expression patterns of genes known to be required for organ development in the developing chick embryo in order to identify genes also involved in liver development. To date, we have found sixteen genes that are expressed in the developing chick liver (GELD, genes expressed in liver development). This knowledge will be fundamental to the establishment of the basic technology for engineering liver tissue in the future. [source]

Most readily usable methods to measure ocular blood flow

Purpose SIS Lecture. Methods Literature search. Results Ocular Blood Flow Research Association (OBFRA, recently merged with another organization - ISOCO, into one single Association for Ocular CDirculation - AOC) made a significant contribution to standardization of the blood flow measuring techniques in the field of ophthalmology. A consens was reached on the number of OBF measurements techniques that occured in the past decades. Particular emphasis was placed on the basic technology, specific parameters and interpretation, accuracy and reproducibility, field of clinical applications. Open questions were extensively discussed, limits of each technique clearly postulated. and a consensus statement put together for each of the technique involved. It encompassed techniques like color Doppler imaging, laser Doppler flowmetry (continuous as well as scanning LDF), laser Doppler velocimetry, Retinal Vessel Analyzer, combination of the vessel diameter measurement and the LDV, laser interferometry of the fundus pulsations amplitude, retinal oxymetry, measurements of the pulsatile component of the blood flow, blue field entoptic method and the newest - Doppler OCT. Conclusion There is no overwhelming measuring technique able to cover all the aspects of the research and the daily clinical routine. Various parameters and various vascular beds are involved, which makes the interpretation of the obtained results strenuous. Of particular importance is the capability of OBF measuring techniques to capture one dynamic feature of ocular circulation - its ability to regulate and to response to various challenges. It is widely believed that not the constantly reduced blood flow, but rather the lack of regulation thereof, leads to prevalent ocular diseases. [source]