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Test Panel (test + panel)
Selected AbstractsRelease liner removal method for transdermal drug delivery systems (TDDS)JOURNAL OF PHARMACEUTICAL SCIENCES, Issue 7 2010Anna M. Wokovich Abstract A release liner removal test is a valuable test for assessing the quality of a transdermal drug delivery system (i.e., TDDS, patch). This test measures the force required to remove the release liner from a patch. The objective of the present study was to establish sample preparation and instrument parameters for measuring release liner removal adhesion for TDDS. Ten TDDS were evaluated (six drugs for a total of 29 lots). Patches which had a rate-controlling membrane were run as-is, since they could not be cut to a precise width without sacrificing their structural integrity. Patches that were square or rectangular in shape were run as-is, and the width of these patches was determined using a digital caliper. Patches which were not square or rectangular in shape and did not have a rate-controlling membrane were cut to a precise width using a specimen cutter. Double-sided tape was used to adhere the liner side of the transdermal system to a clean stainless steel test panel. A release liner peel adhesion method for TDDS is proposed using a dwell time of approximately 3,min, a peel angle of 90°, and a peel speed of 300,mm/min. © 2010 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 99:3177,3187, 2010 [source] Natural rubber latex and chestnut allergy: cross-reactivity or co-sensitization?ALLERGY, Issue 11 2007M. Raulf-Heimsoth Background:, Chestnut and natural rubber latex (NRL) allergy are often associated in the latex-fruit syndrome. Aim of the study:, To establish whether the concurrent NRL and chestnut IgE antibody reactivity are the results of co-sensitization or cross-reactivity. Methods:, Sera from 19 patients with chestnut- and NRL-specific IgE were selected and tested for reactivity with recombinant (r) latex allergens. Cross-reactivity was explored by IgE-inhibition experiments using chestnut or NRL allergens as solid phase on ImmunoCAP. Results:, IgE-antibodies were detected to rHev b 6.01 (prohevein) in 58% of the sera, to rHev b 5 in 32%, to rHev b 12 in four of 13 sera, to rHev b 7.02 and rHev b 11 in four, and to rHev b 1 in two of 19 sera. rHev b 8-IgE antibodies were found in nine sera (47%), whereas six displayed mono-sensitization to rHev b 8 with regard to our test panel. Three of 16 sera showed IgE to cross-reactive carbohydrate determinants. In most sera recognizing rHev b 5 and/or rHev b 6.01 as major allergens the IgE-reactivity to NRL remained unaffected by chestnut extract and chestnut-IgE remained unaffected by NRL extract. Conversely, in sera with rHev b 8 as dominant allergen IgE-binding to NRL was nearly completely inhibited by chestnut and vice versa. IgE-binding to rHev b 8 was abolished by chestnut extract. Conclusions:, Although patients have concomitant IgE antibody reactivity to chestnut and NRL, cross-reactivity could be demonstrated mainly in those patients with IgE to Hev b 8 (profilin) from NRL. [source] Late reactions to the patch-test preparations para-phenylenediamine and epoxy resin: a prospective multicentre investigation of the German Contact Dermatitis Research GroupBRITISH JOURNAL OF DERMATOLOGY, Issue 4 2006U. Hillen Summary Background Late patch-test reactions, developing at day (D) 7 or later have been described for several allergens. Late reactions may reflect patch-test sensitization. Para-phenylenediamine (PPD) and epoxy resins (ER) are potent allergens and therefore may potentially induce patch-test sensitization. Up to now, there has been no prospective study on the frequency of late reactions in routine patch testing with these allergens. Objectives To assess the frequency of late reactions to PPD and ER. Patients/methods In 1748 patients PPD (PPD-base, 1% pet.) and ER [based on diglycidylether of bisphenol A (DGEBA, 1% pet.)], and in 812 patients, nickel sulphate (5% pet.) were removed from the test panel of the standard series and applied on the medial side of the upper arm. Patch-test occlusion time was 24 h in 588 (PPD and ER) and 241 patients (nickel sulphate), respectively, and 48 h in 1160 (PPD and ER) and 571 (nickel sulphate) patients, respectively. Patch tests were read on D1,3 and D2,3, respectively; additional late readings were performed on D7, D14 and D21 after patch-test application. Patients who were not able to return for all scheduled late readings were telephoned on D7, D14 or D21, and questioned about a reaction at the test sites. Patients were instructed to perform daily self-examination from D4 onwards and to return immediately to the clinic if a reaction at the upper arm became visible. Results Data of 1428 patients (ER and PPD) and 638 patients (nickel) were evaluable. In 25 patients (1·8%), patch tests became positive not before D7, among them 21 reactions to PPD (1·5%) and four reactions to ER (0·3%). In five of seven patients, repeated patch tests with PPD disclosed patch-test sensitization as the cause of the late reaction. All late reactions, except for one, occurred in patients in whom patch tests were applied for 48 h. No late reactions were seen with nickel sulphate. Conclusions PPD (1% pet.) elicited late reactions in 1·5% of routine patch tests, the majority of them probably being caused by patch-test sensitization. Therefore, the German Contact Dermatitis Research Group decided to remove PPD 1% pet. from the German standard series and to take efforts to optimize the patch-test conditions of PPD. One way to optimize PPD testing could be to reduce the exposure of PPD 1% to 24 h. Alternatively the patch-test concentration of PPD might be reduced. [source] Incidence and severity of marine borer attack at different depths at Mtongwe Jetty Pontoon Mombasa, KenyaAFRICAN JOURNAL OF ECOLOGY, Issue 4 2009P. K. Sirmah Abstract The activity of marine borers was investigated at Mtongwe Jetty Pontoon, Mombasa, Kilindini harbour using untreated panels of Pinus patula (Schlecht) submerged to different depths. Twenty test panels replicated over 7 months were each strung in three ladder-like frames using nylon twine and suspended vertically, in such a way that the top most panels were 25 cm below the water surface at low and high tide. Monthly observations were made between January and July for the vertical incidence and extent of marine borer attack following the technique of Bobat (1995). Marine borers were collected from test panels, identified and counted. In addition, the percentage weight loss for panels at different depths was determined at the end of 7 months. Teredo fulleri (Clapp) was dominant on panels near water surface while Martesia striata (Linne) and Lyrodus pedicellatus (Quatrefages) were predominant at the bottom. The results showed that attack began within the first month of exposure and was severest by the seventh month. The vertical incidence and extent of attack decreased with increasing depth of panel exposure and was negatively correlated with depth. This is attributed to changes in hydrographic conditions. Résumé L'activité des foreurs marins a étéétudiée dans le Ponton de Mtongwe Jetty, Mombasa, dans le port de Kilindini, en utilisant des panneaux de Pinus patula (Schlecht) non traités, plongés à différentes profondeurs. Vingt panneaux tests identiques ont été attachés avec des cordes de nylon pour former trois cadres ressemblant à des échelles et suspendus pendant sept mois verticalement de façon à ce que les panneaux supérieurs se trouvent à 25 cm sous la surface à marée basse et haute. L'on a fait des observations mensuelles entre janvier et juillet de l'occurrence verticale et l'étendue de l'attaque des foreurs marins en utilisant la technique de Bobat (1995). Les foreurs marins ont été collectés sur les panneaux tests, identifiés et comptés. De plus, on a déterminé le pourcentage de la perte de poids des panneaux aux différentes profondeurs à la fin des sept mois. Teredo fulleri (Clapp) était dominant sur les panneaux situés près de la surface de l'eau alors que Martesia striata (Linné) et Lyrodus pedicellatus (Quatrefages) étaient prédominants en profondeur. Les résultats ont montré que l'attaque a commencé dès le premier mois et que c'est alors qu'elle fut la plus sévère. L'occurrence verticale et l'étendue de l'attaque diminuaient quand le panneau était plus profond et étaient négativement liées à la profondeur. Cela est attribué aux changements des conditions hydrographiques. [source] | ||||||||||