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Technical Approaches (technical + approach)
Selected AbstractsFree Right Internal Thoracic Artery in a "Horseshoe" Configuration: A New Technical Approach for "In Situ" Conduit LengtheningJOURNAL OF CARDIAC SURGERY, Issue 6 2005Jacob Zeitani M.D. We present a method to revascularize the left antero-lateral myocardial wall using the right internal thoracic artery as a "free graft" anastomosed side to end to the "in situ" left internal thoracic artery, in a "horseshoe" fashion. The two ends of the "free graft" were anastomosed to the left anterior descending coronary artery and the second obtuse marginal branch, respectively. This method was successfully used in a 74-year-old patient with severe chronic obstructive pulmonary disease. [source] Technical Approach to Simplify the Purification Method and Characterization of Microbial Transglutaminase Produced from Streptoverticillium ladakanumJOURNAL OF FOOD SCIENCE, Issue 1 2000M.-L. Ho ABSTRACT: In order to fast and economically purify MTGase from Streptoverticillium ladakanum, a stepwise elution method was developed and compared with linear gradient elution method. MTGase was purified to electrophoretical homogeneity by using CM Sepharose CL-6B and Blue Sepharose Fast Flow chromatographies by linear gradient or stepwise methods. The recovery of MTGase by linear gradient and stepwise methods were 68.4% and 81.0%, respectively. The optimal temperature and pH were 40 °C and 5.5, respectively. It was stable at pH 5.0 to 7.0 and had a rate constant (KD) of 6.21 °o 10 -5 min -1 for thermal inactivation at 45 °C. The purified MTGase was activated by K+ Na+, Ca2+, Mn2+, and Mg2+, not affected by Fe3+, EDTA, but inhibited by Cu2+, Zn2+, Hg2+, Ni2+, Co2+, Cd2+, PCMB, NEM, IAA, and PMSF. A simple stepwise method was developed for the purification of MTGase from S. ladakanum. [source] Novel identification of expressed genes and functional classification of hypothetical proteins from Neisseria meningitidis serogroup APROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 18 2007Giulia Bernardini Abstract To implement the 2-DE database of serogroup A Neisseria meningitidis (MenA) and improve its potential of investigation in bacterial biology, cell extracts were separated by tricine-SDS-PAGE and 131 novel proteins were identified by ,LC-ESI-IT-MS/MS. These identifications extended to 404, the number of MenA gene expression products characterized at the proteome level, approximately covering 20% of the total ORFs predicted from genome sequence. This technical approach was particularly useful in ascertaining expression of ribosomal as well as hypothetical proteins. Particular attention was paid to functional characterization of hypothetical proteins by means of software analyses and database searches. [source] Technical decomposition approach of critical to quality characteristics for Product Design for Six SigmaQUALITY AND RELIABILITY ENGINEERING INTERNATIONAL, Issue 4 2010Yihai He Abstract Product Design for Six Sigma (DFSS) approach is a structural and disciplined methodology driven by critical to quality characteristics (CTQs). How to identify and decompose the CTQs is the kernel part in the DFSS process. Traditional method only depends on the quality function deployment (QFD) matrix to flow down CTQs roughly. The paper puts forward a novel technical approach for CTQs decomposition from customer requirements into critical technical parameters based on the relational tree. Specifically, this approach emphasizes the systematic process and quantitative computation on quality relation weight. In order to specify the object of product DFSS, the connotation and evolution model of CTQs are created first. Then along the product development process, a decomposition measure for relational tree of CTQs is studied based on the functional and physical trees in Axiomatic Design (AD). And the quality relation weight computation of its nodes by means of Rough Set and fuzzy Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) is explored. Finally, an application on a car body noise vibration harshness (NVH) improvement, as an example, is given, and the decomposition process of NVH related with the functional and physical trees as well as its node weights computation algorithm are expounded in detail. Copyright © 2009 John Wiley & Sons, Ltd. [source] Science, Stewardship, and Spirituality: The Human Body as a Model for Ecological RestorationRESTORATION ECOLOGY, Issue 1 2006Valentin Schaefer Abstract Ecological restoration is practiced by people of diverse backgrounds working at many different levels in a variety of settings. Portraying the human body as a metaphor of a natural ecosystem can be useful in identifying the breadth of strategies used to restore the natural environment. A technical approach, environmental stewardship, and more spiritual traditional attitudes to ecological restoration can be viewed as analogous to restoring health to the human body through an operation in a hospital, administering remedies to oneself from the medicine cabinet, and taking a broader holistic/preventative approach to cultivate the mind,body connection through, e.g., yoga. [source] Computer-assisted navigation applied to fetal cardiac interventionTHE INTERNATIONAL JOURNAL OF MEDICAL ROBOTICS AND COMPUTER ASSISTED SURGERY, Issue 3 2007Stephen P. Emery Abstract Background Prenatal cardiac interventions (PCI) for human fetal aortic valve (AoV) stenosis can reduce left ventricular hypoplasia and restore ventricular growth and function. However, ,freehand' needle delivery from the maternal skin through the uterine wall, fetal chest and ventricular apex to cross the fetal AoV remains technically challenging and time intensive, and is the rate-limiting step in the procedure. Methods We developed a computer-assisted navigation (CANav) system that tracks the position and orientation of a two-dimensional (2D) ultrasound image relative to the trajectory of an electromagnetic (EM) embedded needle and stylet. We tested the CANav system in vitro using a water bath phantom, then in vivo using adult rats and pregnant (fetal) sheep. Results The CANav system accurately tracked the delivered needle position in both in vitro phantom and adult rat model experiments. We performed 22 PCI attempts with or without CANav in a fetal sheep model. Maternal laparotomy was required to adjust the fetal position in 50% of the procedures. The time required to deliver the needle from the skin into the left ventricle (LV) using CANav was 2.9 ± 1.7 (range 2,7) min (n = 14) vs. 5.5 ± 4.3 (range 1,12) min (n = 8) without CANav (p < 0.05). The time needed to cross the aortic valve once the needle was within the LV was similar with and without CANav (p = 0.19). Conclusions CANav reduces the PCI time required to accurately deliver a needle to the fetal heart. Adaptations of this technical approach may be relevant to other congenital cardiac conditions and ultrasound-guided medical procedures. Copyright © 2007 John Wiley & Sons, Ltd. [source] Grids challenged by a Web 2.0 and multicore sandwichCONCURRENCY AND COMPUTATION: PRACTICE & EXPERIENCE, Issue 3 2009Geoffrey Fox Abstract We discuss the application of Web 2.0 to support scientific research (e-Science) and related ,e-more or less anything' applications. Web 2.0 offers interesting technical approaches (protocols, message formats, and programming tools) to build core e-infrastructure (cyberinfrastructure) as well as many interesting services (Facebook, YouTube, Amazon S3/EC2, and Google maps) that can add value to e-infrastructure projects. We discuss why some of the original Grid goals of linking the world's computer systems may not be so relevant today and that interoperability is needed at the data and not always at the infrastructure level. Web 2.0 may also support Parallel Programming 2.0,a better parallel computing software environment motivated by the need to run commodity applications on multicore chips. A ,Grid on the chip' will be a common use of future chips with tens or hundreds of cores. Copyright © 2008 John Wiley & Sons, Ltd. [source] World Bank Influence and Institutional Reform in ArgentinaDEVELOPMENT AND CHANGE, Issue 3 2009Maria F. Tuozzo ABSTRACT During the 1990s, reforms concerned with ,good governance' became popular with multilateral and bilateral lenders. This trend was led by the World Bank, which claimed that in order to achieve economic development, institutions mattered. This article looks at governance reforms in Argentina, specifically in the judicial sector, and contends that World Bank involvement affected the nature, reach and depth of these initiatives. The influence of the Bank can be traced through three dimensions that have characterized its approach to institutional reform: donor-driven designs for project reform; reliance on technical approaches; and restricted forms of decision making in project initiatives. Such an approach to institutional change conditioned domestic reform in Argentina and contributed to piecemeal and inadequate initiatives. The author also argues that the Bank's approach in Argentina can be traced to wider strategies that derive from embedded institutional practices and ideological foundations within the institution that throw into question the Bank's capacities to promote such reforms. [source] Prevalence of BRCA1 genomic rearrangements in a large cohort of Italian breast and breast/ovarian cancer families without detectable BRCA1 and BRCA2 point mutationsGENES, CHROMOSOMES AND CANCER, Issue 9 2006Simona Agata The presence of genomic rearrangements of the BRCA1 gene in breast and/or ovarian cancer families has been intensively investigated in patients from various countries over the last years. A number of different rearrangements have been reported by several studies that clearly document the involvement of this mutation type in genetic predisposition to breast and ovarian cancer. Population-specific studies are now needed to evaluate the prevalence of genomic rearrangements before deciding whether to include ad hoc screening procedures into standard diagnostic mutation detection approaches. Indeed, the vast majority of the studies have been performed on small, highly selected, sample sets because of the limitations imposed by the laborious technical approaches. Moreover, prevalence figures are likely to differ across different countries according to the ethnic origin of each specific population. Here we analyze a large cohort of 653 Italian probands, negative for BRCA1 and BRCA2 point mutations, gathered from four National Institutions. We report the identification of BRCA1 genomic rearrangements in 12 independent families. Noteworthy, half of the probands carry mutations that recur in more than one Italian family. Considering the whole spectrum of Italian BRCA1 gene rearrangements identified thus far in consecutive patients, we estimate that alterations of this type account for 19% (95% CI: 0.11 < 0.19 < 0.28) of the BRCA1 mutation positive families. We conclude that the search for major genomic rearrangements is essential for an accurate and comprehensive BRCA1 mutation detection strategy in Italy. © 2006 Wiley-Liss, Inc. [source] Evolution of the amygdaloid complex in vertebrates, with special reference to the anamnio-amniotic transitionJOURNAL OF ANATOMY, Issue 2 2007Nerea Moreno Abstract Numerous studies over the last few years have demonstrated that the amygdaloid complex in amniotes shares basic developmental, hodological and neurochemical features. Furthermore, homologous territories of all the main amygdaloid subdivisions have been recognized among amniotes, primarily highlighted by the common expression patterns for numerous developmental genes. Thus, derivatives from the lateral pallium, ventral pallium and subpallium constitute the fundamental parts of the amygdaloid complex. With the development of new technical approaches, study of the precise neuroanatomy of the telencephalon of the anuran amphibians (anamniotes) has been possible. Current embryological, hodological and immunohistochemical evidence strongly suggests that most of the structures present in amniotes are recognizable in these anamniotes. These investigations have yielded enough results to support the notion that the organization of the anuran amygdaloid complex includes subdivisions with their origin in ventral pallial and subpallial territories; a strong relationship with the vomeronasal and olfactory systems; abundant intra-amygdaloid connections; a main output centre involved in the autonomic system; recognizable amygdaloid fibre systems; and distinct chemoarchitecture. Therefore, the new ideas regarding the amygdaloid evolution based on the recent findings in anamniotes, and especially in anurans, strongly support the notion that basic amygdaloid structures were present at least in the brain of ancestral tetrapods organized following a basic plan shared by tetrapods. [source] Basic Rules of Dosimetry in Endovascular BrachytherapyJOURNAL OF INTERVENTIONAL CARDIOLOGY, Issue 6 2000PHILIPPE A. COUCKE M.D. Endovascular brachytherapy after percutaneous coronary intervention (PCI), is becoming a standard approach for the treatment and prevention of restenosis. A variety of technical approaches are currently available to deliver ionizing irradiation to the vascular target. Basically two kinds of radioactive isotopes are available that emit gamma radiation (photons) or beta radiation (electrons). The pitfalls and solutions for the optimization of dosimetry are discussed. As might be expected, the inhomogeneous dose distribution across the target volume results in recurrence by underdosage or in complications because of overdosage. Moreover, uniformization of the target definition and reporting of the dose distribution in endovascular brachytherapy is a prerequisite for comparison between the results of the various clinical trials and is absolutely necessary to improve the therapeutic efficacy of this new approach in the prevention of restenosis after coronary angioplasty with or without stenting. [source] Centre credentialing for Trans Tasman Radiation Oncology Group trial 06.02: multicentre feasibility study of accelerated partial breast irradiationJOURNAL OF MEDICAL IMAGING AND RADIATION ONCOLOGY, Issue 4 2009T Kron Summary Introduction:, Inconsistencies in contouring target volumes for partial breast irradiation (PBI) may result in geographical misses and compromise treatment outcomes. The present study aimed to (1) determine the variability of the target volumes contoured and treatment plans generated by participating centres in credentialing for participation in a multicentre PBI trial; and (2) assess dosimetric changes when standardized target volumes were used. Methods:, The CT image sets of two de-identified patients post-breast conserving surgery were used. Contouring of the target volumes for the two cases was performed and a treatment plan as per protocol specifications was generated for each case by the seven participating centres. Planning of both cases was repeated by five centres using a set of standardized target volumes to evaluate resulting dosimetric changes in the treatment plans. Results:, The surgical cavity, the part of the planning target volume used for dose evaluation and ipsilateral whole breast volumes contoured by the centres varied by 25%, 16% and 21% (1 standard deviation), respectively. The dosimetric variations found when the standardized target volumes were used were smaller than those noted when centre-specific volumes were used. The volumes of the ipsilateral lungs receiving 30% of the prescribed dose and the volumes of the ipsilateral whole breasts receiving 95% and 50% of the prescribed dose were reduced in the treatment plans developed using the standardized target volumes. Conclusions:, Given the impact of contouring on dose distributions, quality assurance procedures in clinical trials of PBI need to take into account both the technical approaches and the contouring. [source] Theoretical and technical approaches to the clinical case: Advantages and disadvantages of present-day pluralism,THE INTERNATIONAL JOURNAL OF PSYCHOANALYSIS, Issue 6 2004ALAN D. FELIX First page of article [source] The PTEN gene in locally progressive prostate cancer is preferentially inactivated by bi-allelic gene deletionTHE JOURNAL OF PATHOLOGY, Issue 5 2006PCMS Verhagen Abstract PTEN is frequently inactivated during the development of many cancers, including prostate cancer, and both bi-allelic and mono-allelic PTEN inactivation may contribute to tumorigenesis. PTEN mutations in clinical cancer specimens can easily be recorded but mono- or bi-allelic gene deletions are often difficult to assess. We performed a comprehensive study to detect PTEN inactivation in 40 locally progressive clinical prostate cancer specimens obtained by transurethral resection of the prostate, utilizing a variety of complementary technical approaches. The methods to detect PTEN deletion included allelotype analysis, dual-colour FISH and array-based CGH. We also applied a novel semi-quantitative approach, assessing the PTEN-WT (wild-type): PTEN- , (pseudogene) ratio (WPR). Structural analysis of PTEN was performed by single-strand conformational polymorphism (PCR-SSCP) and sequencing. PTEN protein expression was assessed by immunohistochemistry. Our data predict complete PTEN inactivation in 12 samples (30%), nine of these by bi-allelic deletion. Loss of one PTEN copy was also detected by several methodologies but the number could not be accurately assessed. Immunohistochemistry indicated the absence of PTEN protein in 15 samples, and heterogeneous expression of the protein in eight tumours. Taken together, these data show that bi-allelic deletion is a major mechanism of PTEN inactivation in locally progressive prostate cancer. Copyright © 2006 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. [source] Context and its significance in identifying ,what works' in child protectionCHILD ABUSE REVIEW, Issue 3 2005Adrian Barton Abstract The repetitive nature of the underlying problems with child protection systems identified by many inquiries into child deaths suggests that the ability to transpose successful strategies from one area to another may be contingent on more than a ,technical' approach to best practice. Current policy responses to failing child protection systems are arguably based on an assumption that practices that work in one area may be applied in other areas without reference to the existing base for practice. Drawing on our own experiences in the field, we attempt to explore some methodological issues relevant to the evaluation of service provision and the dissemination of effective practice in interagency working. Copyright © 2005 John Wiley & Sons, Ltd. [source] | |||||||||||||||||||