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Tea Tree Oil (tea + tree_oil)
Selected AbstractsInhibition of acetylcholinesterase activity by tea tree oil and constituent terpenoidsFLAVOUR AND FRAGRANCE JOURNAL, Issue 2 2006Mitsuo Miyazawa Abstract In vitro inhibition of bovine erythrocyte acetylcholinesterase (AChE) activity by tea tree oil was investigated. The main constituents in the tea tree oil batch used for the analysis of AChE inhibition were terpinen-4-ol (35.6%), , -terpinene (19.5%), , -terpinene (8.3%), p -cymene (7.2%) and 1,8-cineole (4.4%). AChE was measured by a colorimetric method. IC50 values were obtained for tea tree oil and , -pinene and were 51.2 µg/ml and 57.1 µg/ml, respectively. Tea tree oil was found to contain mixed-type inhibitors; a mixture of main constituents and main constituents showed competitive inhibition. Copyright © 2005 John Wiley & Sons, Ltd. [source] Inhibition of acetylcholinesterase activity by tea tree oil and constituent terpenoidsFLAVOUR AND FRAGRANCE JOURNAL, Issue 6 2005Mitsuo Miyazawa Abstract In vitro inhibition of bovine erythrocyte acetylcholinesterase activity by tea tree oil was investigated. The main constituents in the tea tree oil batch used for analysis of acetylcholinesterase inhibition were terpinen-4-ol (35.6%), , -terpinene (19.5%), , -terpinene (8.3%), p -cymene (7.2%) and 1,8-cineole (4.4%). AChE was measured by a colorimetric method. IC50 values were obtained for tea tree oil and , -pinene and were 51.2 and 57.1 µg[sol ]ml, respectively. Tea tree oil was found to comprise of mixed type inhibitors as the main constituents. The main constituents were competitive inhibitors. Copyright © 2005 John Wiley & Sons, Ltd. [source] Tea tree oil concentration in follicular casts after topical delivery: Determination by high-performance thin layer chromatography using a perfused bovine udder modelJOURNAL OF PHARMACEUTICAL SCIENCES, Issue 2 2005S.S. Biju Abstract Tea tree oil, a popular antimicrobial agent is recommended for the treatment of acne vulgaris, a disease of the pilosebaceous unit. Tea tree oil formulations (colloidal bed, microemulsion, multiple emulsion, and liposomal dispersion containing 5% w/w tea tree oil) were applied to bovine udder skin. The follicular uptake of tea tree oil upon application was determined by a cyanoacrylate method. Tea tree oil was determined by quantifying terpinen-4-ol content using high-performance thin layer chromatography. The accumulation of tea tree oil in the follicular casts was 0.43,±,0.01, 0.41,±,0.009, 0.21,±,0.006, and 0.16,±,0.005 percentage by weight (milligram oil/gram of sebum plug) for microemulsion, liposomal dispersion, multiple emulsion, and colloidal bed, respectively. This is the first study of its kind to quantify tea tree oil concentration in the follicles. © 2004 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 94:240,245, 2005 [source] Tea tree oil reduces histamine-induced skin inflammationBRITISH JOURNAL OF DERMATOLOGY, Issue 6 2002K.J. Koh Summary Background Tea tree oil is the essential oil steam-distilled from Melaleuca alternifolia , an Australian native plant. In recent years it has become increasingly popular as an antimicrobial for the treatment of conditions such as tinea pedis and acne. Objectives ,To investigate the anti-inflammatory properties of tea tree oil on histamine-induced weal and flare. Methods Twenty-seven volunteers were injected intradermally in each forearm (study and control assigned on an alternating basis) with histamine diphosphate (5 µg in 50 µL). Flare and weal diameters and double skin thickness were measured every 10 min for 1 h to calculate flare area and weal volume. At 20 min, 25 µL of 100% tea tree oil was applied topically to the study forearm of 21 volunteers. For six volunteers, 25 µL paraffin oil was applied instead of tea tree oil. Results Application of liquid paraffin had no significant effect on histamine-induced weal and flare. There was also no difference in mean flare area between control arms and those on which tea tree oil was applied. However, mean weal volume significantly decreased after tea tree oil application (10 min after tea tree oil application, P = 0·0004, Mann,Whitney U-test). Conclusions ,This is the first study to show experimentally that tea tree oil can reduce histamine-induced skin inflammation. [source] In vitro study on the anticandidal activity of Melaleuca alternifolia (tea tree) essential oil combined with chitosanFLAVOUR AND FRAGRANCE JOURNAL, Issue 4 2008Claudia Juliano Abstract The in vitro activities of commercial Melaleuca alternifolia essential oil (tea tree oil; TTO) and chitosan were investigated against Candida albicans, both alone and in combination, to assess their potential synergistic action; their anticandidal properties were assessed using standard microbiological techniques, such as MIC evaluation and a ,time,kill' test. Our results demonstrated that both TTO and chitosan possess remarkable activity against Candida: TTO inhibited C. albicans at 0.5 % v/v and was able to kill yeast cells in 120,min at 1% and in 300 min at 0.5,0.25%; as far as chitosan was concerned, 0.5 mg/ml were required to inhibit Candida growth in liquid medium, whereas 0.5,2 mg/ml chitosan required 24 h to kill them in the ,time,kill' test; this effect was pH-dependent, being present at pH 5.0 and disappearing at pH 2.0. When TTO and chitosan were tested together, the polymer reduced the MIC of TTO but did not enhance the activity of the oil as evaluated in the ,time,kill' test. Copyright © 2008 John Wiley & Sons, Ltd. [source] Inhibition of acetylcholinesterase activity by tea tree oil and constituent terpenoidsFLAVOUR AND FRAGRANCE JOURNAL, Issue 2 2006Mitsuo Miyazawa Abstract In vitro inhibition of bovine erythrocyte acetylcholinesterase (AChE) activity by tea tree oil was investigated. The main constituents in the tea tree oil batch used for the analysis of AChE inhibition were terpinen-4-ol (35.6%), , -terpinene (19.5%), , -terpinene (8.3%), p -cymene (7.2%) and 1,8-cineole (4.4%). AChE was measured by a colorimetric method. IC50 values were obtained for tea tree oil and , -pinene and were 51.2 µg/ml and 57.1 µg/ml, respectively. Tea tree oil was found to contain mixed-type inhibitors; a mixture of main constituents and main constituents showed competitive inhibition. Copyright © 2005 John Wiley & Sons, Ltd. [source] Inhibition of acetylcholinesterase activity by tea tree oil and constituent terpenoidsFLAVOUR AND FRAGRANCE JOURNAL, Issue 6 2005Mitsuo Miyazawa Abstract In vitro inhibition of bovine erythrocyte acetylcholinesterase activity by tea tree oil was investigated. The main constituents in the tea tree oil batch used for analysis of acetylcholinesterase inhibition were terpinen-4-ol (35.6%), , -terpinene (19.5%), , -terpinene (8.3%), p -cymene (7.2%) and 1,8-cineole (4.4%). AChE was measured by a colorimetric method. IC50 values were obtained for tea tree oil and , -pinene and were 51.2 and 57.1 µg[sol ]ml, respectively. Tea tree oil was found to comprise of mixed type inhibitors as the main constituents. The main constituents were competitive inhibitors. Copyright © 2005 John Wiley & Sons, Ltd. [source] Tea tree oil concentration in follicular casts after topical delivery: Determination by high-performance thin layer chromatography using a perfused bovine udder modelJOURNAL OF PHARMACEUTICAL SCIENCES, Issue 2 2005S.S. Biju Abstract Tea tree oil, a popular antimicrobial agent is recommended for the treatment of acne vulgaris, a disease of the pilosebaceous unit. Tea tree oil formulations (colloidal bed, microemulsion, multiple emulsion, and liposomal dispersion containing 5% w/w tea tree oil) were applied to bovine udder skin. The follicular uptake of tea tree oil upon application was determined by a cyanoacrylate method. Tea tree oil was determined by quantifying terpinen-4-ol content using high-performance thin layer chromatography. The accumulation of tea tree oil in the follicular casts was 0.43,±,0.01, 0.41,±,0.009, 0.21,±,0.006, and 0.16,±,0.005 percentage by weight (milligram oil/gram of sebum plug) for microemulsion, liposomal dispersion, multiple emulsion, and colloidal bed, respectively. This is the first study of its kind to quantify tea tree oil concentration in the follicles. © 2004 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 94:240,245, 2005 [source] Herbal medicines for treatment of fungal infections: a systematic review of controlled clinical trialsMYCOSES, Issue 3-4 2004Karen W. Martin Antimykotische Chemotherapie; Phytomedizin Summary Traditional medicine has made use of many different plant extracts for treatment of fungal infections and some of these have been tested for in vitro antifungal activity. This systematic review evaluates antifungal herbal preparations that have been tested in controlled clinical trials. Four electronic databases were searched for controlled clinical trials of antifungal herbal medicines. Data were extracted in a standardized manner by two independent reviewers and are reviewed narratively. Seven clinical trials met our inclusion criteria. Tea tree oil preparations were tested in four randomized clinical trials and some positive outcomes were attributed to the intervention in all trials. Solanum species (two trials) and oil of bitter orange preparations (one trial) were compared with conventional treatments. In all cases encouraging results were reported. There are few controlled clinical trials of herbal antifungal medicines. The most thoroughly clinically tested is tea tree oil, which holds some promise. All herbal remedies require further investigation in rigorous clinical trials. Zusammenfassung Die traditionelle Medizin nutzt eine Vielzahl unterschiedlicher Pflanzenextrakte zur Behandlung von Pilzinfektionen, die teilweise auf antimyzetische Wirksamkeit in vitro untersucht wurden. Dieser Überblick bewertet diejenigen antimyzetischen Zubereitungen pflanzlichen Ursprungs, die in kontrollierten klinischen Studien geprüft worden sind. Zu diesem Zweck wurden vier elektronische Datenbanken gesichtet. Die Daten wurden mit einer standardisierten Methode von zwei unabhängigen Gutachtern erhoben und werden im Folgenden bewertend dargestellt. Sieben klinische Studien erfüllten unsere Einschlusskriterien. Teebaumöl-Zubereitungen wurden in vier randomisierten klinischen Studien getestet, und einige positive Ergebnisse wurden in allen Studien auf den Wirkstoff zurückgeführt. Zubereitungen von Solanum -Arten (zwei Studien) und Orangenbitteröl wurden mit konventionellen Behandlungsmethoden verglichen. In allen Studien wurden ermutigende Resultate erzielt. Diese wenigen kontrollierten klinischen Studien mit antimyzetischen Zubereitungen pflanzlichen Ursprungs ergaben, dass Teebaumöl am vielversprechendsten ist. Alle pflanzlichen Zubereitungen erfordern jedoch weitere Studien unter kritischen klinischen Versuchbedingungen. [source] Tea tree oil reduces histamine-induced skin inflammationBRITISH JOURNAL OF DERMATOLOGY, Issue 6 2002K.J. Koh Summary Background Tea tree oil is the essential oil steam-distilled from Melaleuca alternifolia , an Australian native plant. In recent years it has become increasingly popular as an antimicrobial for the treatment of conditions such as tinea pedis and acne. Objectives ,To investigate the anti-inflammatory properties of tea tree oil on histamine-induced weal and flare. Methods Twenty-seven volunteers were injected intradermally in each forearm (study and control assigned on an alternating basis) with histamine diphosphate (5 µg in 50 µL). Flare and weal diameters and double skin thickness were measured every 10 min for 1 h to calculate flare area and weal volume. At 20 min, 25 µL of 100% tea tree oil was applied topically to the study forearm of 21 volunteers. For six volunteers, 25 µL paraffin oil was applied instead of tea tree oil. Results Application of liquid paraffin had no significant effect on histamine-induced weal and flare. There was also no difference in mean flare area between control arms and those on which tea tree oil was applied. However, mean weal volume significantly decreased after tea tree oil application (10 min after tea tree oil application, P = 0·0004, Mann,Whitney U-test). Conclusions ,This is the first study to show experimentally that tea tree oil can reduce histamine-induced skin inflammation. [source] | |||||||||||||