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Swine
Kinds of Swine Terms modified by Swine Selected AbstractsDevelopment of a Novel Animal Burn Model Using Radiant Heat in Rats and SwineACADEMIC EMERGENCY MEDICINE, Issue 5 2010Reuven Gurfinkel MD Abstract Objectives:, The objective was to develop a novel animal model of burns in rats and pigs. Methods:, The model uses heat that is delivered via a radiant heater with an opening of 5 cm by 5 cm, set at 400°C, for 20 seconds. An advantage of this model is that the heating source does not come into direct contact with the animal, and the heat dispersion surrounding its center is very constant. The device was evaluated in 40 rats and seven pigs. With rats, three to four burns were created on each rat, resulting in a burn covering a total body surface area of 30% to 50%. In pigs, 16 burns were created on each animal. Results:, In rats, infliction of burns resulted in mortality rates of 0%,50% depending on the size of the burns and the rats. In pigs, the burns reepithelialized within approximately 3 weeks and resulted in hourglass contracted scars in two of three burns within 1 month. Conclusions:, The authors describe a novel animal burn model that utilizes radiant heat to create consistent burns that maximizes safety to the investigators and animals. ACADEMIC EMERGENCY MEDICINE 2010; 17:514,520 © 2010 by the Society for Academic Emergency Medicine [source] Twenty-Four Hours Postoperative Results After Orthotopic Cardiac Transplantation in SwineJOURNAL OF CARDIAC SURGERY, Issue 4 2007Matthias Siepe M.D. However, there is no functional data available for a longer time period after transplantation. We have established a pig model to investigate myocardial function 24 hours after orthotopic transplantation.Materials and Methods: Orthotopic cardiac transplantations (HTx) in pigs were performed with a postoperative observation period of 24 hours (n = 6). To analyze myocardial function after transplantation, hemodynamical parameters (Swan-Ganz- and impedance-catheter data) as well as tissue and blood samples were obtained. Regional myocardial blood flow (RMBF) was assessed using fluorescent microspheres. Results: The impedance-catheter parameters demonstrated a preserved contractility in both ventricles 24 hours post-transplantation. In contrast, cardiac output 24 hours after HTx was diminished by 50% as compared to the preoperative value. Conversely, pulmonary vascular resistance increased significantly. The RMBF was increased in both ventricles. Metabolic and histological analyses indicate myocardial recovery 24 hours after HTx with no irreversible damage. Conclusions: For the first time, we were able to establish a porcine model to investigate myocardial function 24 hours after heart transplantation. While the contractility of the transplanted hearts was well-preserved, impaired cardiac output was going along with an increase in pulmonary vascular resistance. Using this clinical relevant model, improvements of human cardiac transplantation and post-transplant contractile dysfunction, especially, could be investigated. [source] Serological markers of hepatitis B, C, and E viruses and human immunodeficiency virus type-1 infections in pregnant women in Bali, IndonesiaJOURNAL OF MEDICAL VIROLOGY, Issue 4 2005I Gede Putu Surya Abstract Except for hepatitis B virus (HBV), there have been few data on serological markers of hepatitis viruses such as hepatitis C virus (HCV) and E virus (HEV), and human immunodeficiency virus type-1 (HIV) in Bali, Indonesia. During 5 months from April to August 2003, sera were collected from 2,450 pregnant women at eight jurisdictions in Bali, and they were tested for markers of these viruses. Only one (0.04%) was positive for antibody to HCV, but none for antibody to HIV. Hepatitis B surface antigen (HBsAg) was detected in 46 (1.9%) at a prevalence significantly lower than that in 271 of the 10,526 (2.6%) pregnant women in Bali surveyed 10 years previously (P,<,0.045). The prevalence of hepatitis B e antigen in pregnant women with HBsAg decreased, also, from 50% to 28% during the 10 years (P,<,0.011). Antibody to HEV (anti-HEV) was examined in 819 pregnant women who had been randomly selected from the 2,450. The overall prevalence of anti-HEV was 18%, and there were substantial regional differences spanning from 5% at Tabanan district to 32% at Gianyar district. Furthermore, the prevalence of anti-HEV differed substantially by their religions. In the Sanglah area of Denpasar City, for instance, anti-HEV was detected in 20 of the 102 (20%) Hindus, significantly more frequently than in only 2 of the 101 (2.0%) Muslims (P,<,0.001). Swine that are prohibited to Muslims, therefore, is likely to serve as a reservoir of HEV in Bali. In conclusion, HBV is decreasing, HCV and HIV have not prevailed, as yet, while HEV is endemic probably through zoonotic infection in Bali. J. Med. Virol. 75:499,503, 2005. © 2005 Wiley-Liss, Inc. [source] Continuous Vagus Nerve Stimulation Effects on the Gut-Brain Axis in SwineNEUROMODULATION, Issue 1 2007Idoia Díaz-Güemes DVM ABSTRACT Objectives., This study was designed to assess vagus nerve stimulation effects on the food intake pattern in swine and determine the electrical stimulus direction. Material and Methods., Fifteen Large White pigs were randomly divided into three groups, groups A,C. All animals underwent implantation of a vagus nerve stimulator at the gastro-esophogeal junction. In group A, the stimulation was switched off, whereas stimulation was switched on in groups B and C. Food intake and body weight were registered in groups A and B, but not in group C, which was used to measure direction of stimulation in the vagus and effect on heart rate and blood pressure. Variables measured in group C included the bispectral index, blood pressure, and heart rate. A Student's t -test and one-way analysis of variance were used to detect differences between groups. All animals were sacrificed to identify effects of implantation and stimulation on the vagus nerve. Results., With respect to food intake, there was no difference between groups A and B; however, body weight did register a continuous increase. During stimulation, in group C arterial pressures decreased significantly, whereas the heart rate and bispectral index increased. Conclusion., The stimulation protocol applied in this study was insufficient to cause changes in the feeding behavior of swine; however, it did increase central nervous system activity. [source] Extracorporeal Support: Improves Donor Renal Graft Function After Cardiac DeathAMERICAN JOURNAL OF TRANSPLANTATION, Issue 6 2010A. Rojas-Pena Donors after cardiac death (DCD) could increase the organ pool. Data supports good long-term renal graft survival. However, DCDs are <10% of deceased donors in the United States, due to delayed graft function, and primary nonfunction. These complications are minimized by extracorporeal support after cardiac death (ECS-DCD). This study assesses immediate and acute renal function from different donor types. DCDs kidneys were recovered by conventional rapid recovery or by ECS, and transplanted into nephrectomized healthy swine. Warm ischemia of 10 and 30 min were evaluated. Swine living donors were controls (LVD). ECS-DCDs were treated with 90 min of perfusion until organ recovery. After procurement, kidneys were cold storage 4,6 h. Renal vascular resistance (RVR), urine output (UO), urine protein concentration (UrPr) and creatinine clearance (CrCl), were collected during 4 h posttransplantation. All grafts functioned with adequate renal blood flow for 4 h. RVR at 4 h posttransplant returned to baseline only in the LVD group (0.36 mmHg/mL/min ± 0.03). RVR was higher in all DCDs (0.66 mmHg/mL/min ± 0.13), without differences between them. UO was >50 mL/h in all DCDs, except in DCD-30 (6.8 mL/h ± 1.7). DCD-30 had lower CrCl (0.9 mL/min ± 0.2) and higher UrPr >200 mg/dL, compared to other DCDs >10 mL/min and <160 mg/dL, respectively. Normothermic ECS can resuscitate kidneys to transplantable status after 30 min of cardiac arrest/WI. [source] Heavy LYFTing: KASting Pearls Before SwineAMERICAN JOURNAL OF TRANSPLANTATION, Issue 7 2009J. Bromberg The use of "Life Years from Transplant" (LYFT) to allocate kidneys is controversial. Several articles in this issue of AJT present critiques and analysis of KAS and LYFT and provide a road map for future progress. See Special Feature on pages 1500,1532. [source] Indirect Recognition of MHC Class I Allopeptides Accelerates Lung Allograft Rejection in Miniature SwineAMERICAN JOURNAL OF TRANSPLANTATION, Issue 7 2005Tsuyoshi Shoji The role of indirect allorecognition in graft rejection is examined in two experiments using a swine lung transplantation model. First, two swine received class I mismatched grafts without immunosuppression; another two recipients were treated postoperatively with cyclosporine (CsA). These swine exhibited acute and chronic rejection, respectively. All four recipients developed T-cell reactivity to donor-derived class I major histocompatibility complex (MHC) peptides. Second, six swine were immunized with synthetic donor-derived class I allopeptides prior to transplantation. Control groups consisted of nonimmunized recipients (n = 6) and recipients immunized with an irrelevant peptide (n = 3). These recipients all received a 12-day course of post-operative CsA. Swine immunized with allopeptides exhibited accelerated graft rejection, as compared to both control groups (p < 0.01 and p = 0.03, respectively). Within the experimental group, the dominant histologic finding was acute rejection (AR). Obliterative bronchiolitis (OB) was seen in the graft with the longest survival. Both control groups showed a lesser degree of AR, with four out of six nonimmunized swine ultimately developing OB. These studies suggest that indirect allorecognition is operative during lung allograft rejection, and that pre-transplant sensitization to donor-derived MHC allopeptides can accelerate graft rejection. [source] The Hemodynamic Behavior of Arterial Anastomosis Using Fibrin Sealant: Experimental Study in SwineARTIFICIAL ORGANS, Issue 11 2008Eduardo A.V. Rocha Abstract The wide use of biological sealants as a reinforcement for arterial sutures and the small experimental base in literature motivated this study. Our aim was to evaluate the flow, tear pressure, and the need of reinforcement stitches in sutured arteries after a cross-section. This research project complied with the Helsinki convention. The Tissucol (Baxter) fibrin sealant was used in all experiments. The femoral and carotid arteries of 17 swine from the same breed (weighing from 15 to 20 kg) were cross-sectioned after heparinization and subjected to anastomoses using a single continuous plane of 7-0 prolene. We worked with 68 artery samples, 34 in the treatment group and 34 in the control group. For each animal, one carotid and one femoral artery randomly received fibrin sealant with the contralateral side being used as a control. The need and the number of reinforcement stitches were recorded. Ten minutes after protamine infusion, the animals were sacrificed and the arteries were catheterized respecting 1 cm proximal and distal. The arteries were measured and placed on a flow meter to evaluate the flow rate of 10 mL of 0.9% NaCl in a 50 cm high column. The arteries were then subjected to air infusion at increasingly higher pressures (stepwise increases of 25 mm Hg), the grafts were dipped in 0.9% NaCl solution, the first air leakage was observed, and the tear pressure recorded. Data was analyzed with EpiInfo 6 data manager. The external diameters and thickness of the arteries were similar in both the treatment and control group. There was no significant difference between the groups regarding the tear pressure (P = 0.329), flow rate (P = 0.943), and the number of samples with a tear pressure above 200 mm Hg. However, the sealant reduced the number of reinforcement stitches necessary (P = 0.029). We conclude that fibrin sealant reduces the need of additional stitches; however, it does not change the tear pressure nor significantly reduces the flow. [source] Electrophysiology and Hemodynamics of Open Chest Resuscitation from Cardiac Arrest in a SwineACADEMIC EMERGENCY MEDICINE, Issue 1 2009Bphil, David D. Salcido BS First page of article [source] Sensitive Swine and TASER Electronic Control DevicesACADEMIC EMERGENCY MEDICINE, Issue 7 2008Mark W. Kroll PhD No abstract is available for this article. [source] Role of shear stress on nitrite and NOS protein content in different size conduit arteries of swineACTA PHYSIOLOGICA, Issue 2 2009X. Guo Abstract Aim:, Inherent fundamental difference exists among arteries of different sizes. The purpose of this study was to evaluate the relation between regional difference of wall shear stress (WSS) in various sizes arteries and contents of nitrite and NO synthase (NOS) isoforms. Methods:, Five different conduit arteries in a wide range of diameter (1,8 mm) were examined in the hind limbs of 13 pigs. Blood flow rate and outer diameter were measured in vivo to determine WSS. Arterial tissues were harvested for the measurement of nitrite and NOS protein contents. The concentration of nitrite, a product of NO synthesis, was determined by high-performance liquid chromatography method. Western blot analysis was used to assess the protein contents of endothelial NOS (eNOS), inducible NOS (iNOS) and neuronal NOS (nNOS). Results:, Our data show that WSS increases with a decrease in artery diameter. Nitrite level increases with increasing WSS and hence decreases with artery diameter. The eNOS protein contents decrease with an increase in diameter. No significant difference for iNOS and nNOS protein contents was found with different artery diameter. A significant positive correlation between tissue nitrite and eNOS protein contents was also observed. Finally, the WSS-normalized eNOS is not significantly different in various size vessels. Conclusion:, Regional difference in blood flow has no effect on iNOS and nNOS protein contents in these conduit arteries. Regional difference in eNOS expression and nitrite contents may be related to the WSS-induced NO by the endothelium under normal physiological conditions. [source] A Model of Ischemically Induced Ventricular Fibrillation for Comparison of Fixed-dose and Escalating-dose Defibrillation StrategiesACADEMIC EMERGENCY MEDICINE, Issue 6 2004James T. Niemann MD Abstract Objectives: Fixed- and escalating-dose defibrillation protocols are both in clinical use. Clinical observations suggest that the probability of successful defibrillation is not constant across a population of patients with ventricular fibrillation (VF). Common animal models of electrically induced VF do not represent a clinical VF etiology or reproduce clinical heterogeneity in defibrillation probability. The authors hypothesized that a model of ischemically induced VF would exhibit heterogeneous defibrillation shock strength requirements and that an escalating-dose strategy would more effectively achieve prompt defibrillation. Methods:Forty-six swine were randomized to fixed, lower-energy (150 J) transthoracic shocks (group 1) or escalating, higher-energy (200 J,300 J,360 J) shocks (group 2). VF was induced by balloon occlusion of a coronary artery. After 1 or 5 minutes of VF, countershocks with a biphasic waveform were administered. The primary endpoint was successful defibrillation (termination of VF for 5 seconds) with ,3 shocks. Results: VF was induced with occlusion or after reperfusion in 35 animals. Only five of 17 group 1 animals (29%, 95% CI = 10 to 56) could be defibrillated with ,3 shocks; 15 of 18 group 2 animals (83%, 95% CI = 59 to 96) were defibrillated with ,3 shocks (p < 0.002 vs. group 1). Nine of the group 1 animals (75%) that could not be defibrillated with 150-J shocks were rescued with ,3 shocks ranging from 200 to 360 J. Conclusions: In this ischemic VF animal model, defibrillation shock strength requirements varied among individuals, and when defibrillation was difficult, an escalating-dose strategy was more effective for prompt defibrillation than fixed, lower-energy shocks. [source] Enterococcus faecalis with the gelatinase phenotype regulated by the fsr operon and with biofilm-forming capacity are common in the agricultural environmentENVIRONMENTAL MICROBIOLOGY, Issue 6 2009Lilia Macovei Summary The prevalence of gelatinase activity and biofilm formation among environmental enterococci was assessed. In total, 396 enterococcal isolates from swine and cattle faeces and house flies from a cattle farm were screened for gelatinase activity. The most prevalent phenotype on Todd,Hewitt agar with 1.5% skim milk was the weak protease (WP) (72.2% of isolates), followed by the strong protease (SP) 18.7%, and no protease (NP) (9.1%). The majority of WP isolates was represented by Enterococcus hirae (56.9%), followed by Enterococcus faecium (25.9%), Enterococcus casseliflavus (10.4%), Enterococcus gallinarum (5.2%) and Enterococcus saccharolyticus (1.7%). All WP isolates were negative for gelE (gelatinase) and sprE (serine protease) as well as the fsrABDC operon that regulates the two proteases, and only four isolates (7.0%) formed biofilms in vitro. All SP isolates were Enterococcus faecalis positive for the fsrABDC, gelE, sprE genes and the majority (91.2%) formed a biofilm. Diversity of NP isolates was relatively evenly distributed among E. hirae, E. faecium, E. casseliflavus, E. gallinarum, Enterococcus durans, E. saccharolyticus and Enterococcus mundtii. All NP isolates were negative for the fsr operon and only four E. hirae (11.1%) formed a biofilm. Of further interest was the loss of the gelatinase phenotype (18.9% of isolates) from SP isolates after 4 month storage at 4,8°C and several passages of subculture. Results of reverse transcription PCR analysis indicated that mRNA was produced for all the genes in the frs operon and sequencing of the gelE gene did not reveal any significant mutations. However, gelatinase was not detectable by Western blot analysis. Our study shows that E. faecalis with the complete fsr operon and the potential to form a biofilm are relatively common in the agricultural environment and may represent a source/reservoir of clinically relevant strains. In addition, many environmental enterococci, especially E. hirae, produce an unknown WP that can hydrolyse casein but does not contribute to biofilm formation. The stability of the gelatinase phenotype in E. faecalis and its regulation will require additional studies. [source] Impact of animal waste lagoon effluents on chlorpyrifos degradation in soilsENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 12 2000Xinjiang Huang Abstract Animal-derived lagoon effluents are a good source of inorganic nutrients and organic matter; however, they may impact the degradation and transport of soil-applied pesticides. The degradation of chlorpyrifos in poultry-, swine-, and cow-derived effluents and effluent-soil matrices were studied using batch and column incubation studies. Chlorpyrifos was degraded by aerobic microbial processes in animal-derived lagoon effluents. Microbial community analysis by denaturing gradient gel electrophoresis of polymerase chain reaction-amplified 16S ribosomal ribonucleic acid genes showed that a single band became dominant in effluent during chlorpyrifos degradation. In soils, both biotic and abiotic degradation contributed significantly to the overall dissipation of chlorpyrifos. Large differences in degradation rates were observed between soils, with the fastest rate observed in soil with higher pH and cation-exchange capacity. Effluents appeared to have only a minor effect on chlorpyrifos degradation in soils, although effluent-induced increases in soil-solution pH over time may enhance hydrolysis by a few percent in low-pH soils. Soil properties, not effluent properties, appear to control chlorpyrifos degradation under laboratory conditions; however, the impact on changes in soil properties and microbial ecology with long-term effluent irrigation warrants further investigation. [source] Zoonotic risk of hepatitis E virus (HEV): A study of HEV infection in animals and humans in suburbs of BeijingHEPATOLOGY RESEARCH, Issue 12 2009Yibin Chang Aim:, To investigate hepatitis E virus (HEV) infection among different animals and workers in pig farms and slaughterhouses, and analyze the genotype of HEV isolated in this study. Methods:, Serum samples were collected from adult swine, cows, sheep, younger swine (< 3 months), and workers in pig farms and slaughterhouses (professional group). Fecal samples were collected from younger swine in the south suburbs of Beijing. Anti-HEV antibody was evaluated by direct sandwich enzyme immunoassay. HEV RNA was extracted from fecal samples and amplified by nested reverse transcription polymerase chain reaction (RT-nPCR). The PCR products were sequenced, and the sequence homology and phylogenetics of the HEV strains isolated from swine were analyzed. Results:, The anti-HEV positivity rates in adult swine, cows, sheep, younger swine, professional group and general population were 98.23% (222/226), 29.35% (54/184), 9.80% (20/207), 60.73% (99/164), 42.51% (105/247) and 20.29% (522/2572), respectively. The HEV RNA positivity rate of fecal samples was 22.89% (19/83) and 16/19 samples were positive for HEV RNA amplified with both primers, HEV open reading frame (ORF)1 and HEV ORF2. Sequence analysis of these 16 samples showed that there were two groups, designated BJ-1 and BJ-2. The nucleotide homology of BJ-1 and BJ-2 was 99%. Phylogenetic analysis indicated that both of these groups belonged to genotype 4d. Conclusion:, Workers in pig farms and slaughterhouses were more likely to contract HEV infection than the general population because of close contact with swine with a high prevalence of anti-HEV. [source] Irreversible electroporation of the pancreas in swine: a pilot studyHPB, Issue 5 2010Kevin P. Charpentier Abstract Background:, Irreversible electroporation (IRE) is a novel, non-thermal method of tissue ablation using short pulses of high-voltage DC current to ablate tissue. Methods:, Irreversible electroporation of the pancreas was performed in four domestic female swine using two monopolar probes spaced 9,15 mm apart. Ninety pulses of 1500 V/cm were delivered for each ablation. Results:, All animals survived for their designated times of 2 h (n= 1), 2 days (n= 1) and 14 days (n= 2), respectively. No procedure-related complications occurred. Three animals in which probes had been spaced at intervals of 10 ± 1 mm showed evidence of irreversible ablation, with ablation height ranging from <10 mm to 21 mm and ablation width ranging from <10 mm to 16 mm by gross appearance and triphenyltetrazolium chloride (TTC) staining. The only animal in which probes had been spaced at intervals of 15 mm did not show evidence of irreversible ablation at 2 weeks. This may be secondary to the wider probe spacing and relatively low voltage, which results in a mostly reversible form of electroporation without cell death. Conclusions:, Irreversible electroporation appears to be a safe method for pancreas tissue ablation. Staining with TTC can predict the zone of IRE ablation within 2 h of treatment. [source] Development of a swine model of secondary liver tumor from a genetically induced swine fibroblast cell lineHPB, Issue 3 2008R. Abbas Abstract Aim. Metastatic disease is the most common liver tumor. Although alternative therapies have been developed for non-surgical candidates, those therapies lacked ideal testing prior to clinical application because of a paucity of large animal models. The purpose of the present study was to develop a model for secondary liver tumor in a large animal. Material and methods. Fibroblasts were isolated from swine ear lobules and then transfected with amphotrophic retroviruses encoding human or murine genetic material (hTERT, p53DD, cyclinD-1, CDK4R24C, Myc T58A, RasG12V). Transformed cell lines were finally inoculated subcutaneously (s.c.) into: 1) immunodeficient mice (nude), 2) immunocompetent mice (wild type), 3) immunosuppressed swine (under tacrolimus or corticosteroids), 4) immunocompetent swine, and 5) into the liver and portal circulation of swine under steroid-based immunosuppression. Results. In the murine model, tumor growth was evident in 100% of the nude mice (n=5), with a peak size of 20 mm (15.22±4.5 mm; mean±SD) at the time of sacrifice (3 weeks). Tumor growth was evident in 71% of the wild mice (n=21), with a peak size of 7.8 mm (4.19±1.1 mm) by the third week of growth. In the swine model, tumor growth was evident in 75% (3/4 ears; n=2) of swine under tacrolimus-based immunosuppression versus 50% of swine under steroids-based immunosuppression (n=2). Tumor growth was slow in two animals, while in one animal the tumor was larger with a peak growth of 42 mm at 3 weeks. The tumor pattern in the ear lobules was characterized by slow growth, with a peak size of 6,8 mm in the immunocompetent swine at 3 weeks. All tumors were shown to be malignant by histology. In contrast, inoculums of the transformed fibroblast cell line in swine livers showed no evidence of tumor growth at 3 weeks. Conclusions. Development of a transformed swine fibroblast cell line was successful, resulting in an in vivo malignant tumor. Cell line inoculums had tumorigenic properties in nude mice, wild-type mice, and immunosuppressed swine, as judged by uncontrolled cell growth, invasion of surrounding tissue, neoangiogenesis, and invasion of normal vasculature, resulting in the formation of tumor nodules. Such properties were not observed in swine upon inoculation into the liver/portal circulation. [source] Serologic survey of swine workers for exposure to H2N3 swine influenza AINFLUENZA AND OTHER RESPIRATORY VIRUSES, Issue 3 2010Amanda Beaudoin Please cite this paper as: Beaudoin et al. (2010) Serologic survey of swine workers for exposure to H2N3 swine influenza A. Influenza and Other Respiratory Viruses 4(3), 163,170. Background, Of the 16 influenza A hemagglutinin (H) subtypes, only H1, H2 and H3 viruses have been shown to cause sustained human infection. Whereas H1 and H3 viruses currently circulate seasonally in humans, H2 viruses have not been identified in humans since 1968. In 2006, an H2N3 influenza virus was isolated from ill swine in the United States. Objective, To assess the potential for zoonotic influenza transmission, the current study looked for serologic evidence of H2 influenza infection among workers at two swine facilities, some exposed and some unexposed to H2N3-positive pigs. Methods, The sera were assessed for antibodies to swine H2 influenza and currently circulating seasonal human influenza A subtypes H1N1 and H3N2. Workers were interviewed to obtain details such as age, influenza vaccination history, experiences of influenza-like-illness, and use of personal protective equipment and hygiene when working with pigs. Exposure and risk factors for positive antibody titers were compared for exposed and unexposed individuals as well as for H2 antibody-positive and H2 antibody-negative individuals. Results, Blood was taken from 27 swine workers, of whom four had positive H2 antibody titers (,1:40). Three of the positive employees were born before 1968 and one had an unknown birth date. Only one of these workers had been exposed to H2N3-positive pigs, and he was born in 1949. Conclusions, These data do not support the hypothesis that swine workers were infected with the emergent swine H2N3 influenza A virus. [source] Spontaneous delivery during veno-venous extracorporeal membrane oxygenation in swine influenza-related acute respiratory failureACTA ANAESTHESIOLOGICA SCANDINAVICA, Issue 9 2010J. Kunstyr No abstract is available for this article. [source] Identification of five chromosomal regions involved in predisposition to melanoma by genome-wide scan in the MeLiM swine modelINTERNATIONAL JOURNAL OF CANCER, Issue 1 2004Claudine Geffrotin Abstract In human familial melanoma, 3 risk susceptibility genes are already known, CDKN2A, CDK4 and MC1R. However, various observations suggest that other melanoma susceptibility genes have not yet been identified. To search for new susceptibility loci, we used the MeLiM swine as an animal model of hereditary melanoma to perform a genome scan for linkage to melanoma. Founders of the affected MeLiM stock were crossed with each other and with healthy Duroc pigs, generating MeLiM, F1 and backcross families. As we had previously excluded the MeLiM CDKN2A gene, we paid special attention to CDK4 and MC1R, as well as to other candidates such as BRAF and the SLA complex, mapping them on the swine radiation hybrid map and/or isolating close microsatellite markers to introduce them into the genome scan. The results revealed, first, that swine melanoma was inherited as an autosomal dominant trait with incomplete penetrance, preferably in black animals. Second, 4 chromosomal regions potentially involved in melanoma susceptibility were identified on Sus Scrofa chromosomes (SSC) 1, 2, 7 and 8, respectively, in intervals 44,103, 1.9,18, 59,73 and 47,62 cM. A fifth region close to MC1R was revealed on SSC 6 by analyzing an individual marker located at position 7.5 cM. Lastly, CDK4 and BRAF were unlikely to be melanoma susceptibility genes in the MeLiM swine model. The 3 regions on SSC 1, 6 and 7, respectively, have counterparts on human chromosomes (HSA) 9p, 16q and 6p, harboring melanoma candidate loci. The 2 others, on SSC 2 and 8, have counterparts on HSA 11 and 4, which might therefore be of interest for human studies. © 2004 Wiley-Liss, Inc. [source] Inheritance of the F4ab, F4ac and F4ad E. coli receptors in swine and examination of four candidate genes for F4acRJOURNAL OF ANIMAL BREEDING AND GENETICS, Issue 2005P. Python Summary Susceptibility to enterotoxigenic Escherichia coli with fimbriae F4ac is dominantly inherited in the pig. A three-generation pedigree was created to refine the position of F4acR on chromosome 13 comprising 202 pigs: eight parents, 18 F1 and 176 F2 pigs. The 17-point analysis indicates that F4acR lies between Sw207 and S0283. Recombinant offspring specify that the most probable order is Sw207,S0075,F4acR,Sw225,S0283. We observed six phenotypes for the three fimbrial variants F4ab, F4ac and F4ad. The two missing phenotypes F4abR,/F4acR+/F4adR+ and F4abR,/F4acR+/F4adR, indicate that pigs susceptible to F4ac are always susceptible to F4ab. Furthermore, a weak and a strong adhesion of F4ab and F4ad bacteria was observed. The weak receptor F4abR (F4abRw) was present only in pigs devoid of the receptor F4acR (F4abR+/F4acR,). In contrast, in pigs with the phenotype F4abR+/F4acR+, F4ab bacteria adhered to the majority of enterocytes. F4abRw constitutes a frequently observed phenotype whose inheritance is still unclear. Strong adhesion of F4ab and F4ac bacteria is most likely influenced by the same receptor that we name F4bcR. The number of F4ad bacteria that adhered to enterocytes was very variable in the adhesion test. Moreover, expression of F4adR was independent of age. Our segregation analyses indicated a dominant inheritance of F4adR, although the number of susceptible pigs was smaller than expected. We examined four genes as candidates for the F4acR locus: the transferrin receptor gene (TFRC) and three genes members of the glucosyl/galactosyltransferase family (B3GnT5, B3GALT3 and B4GALT4). Comparison of sequences from resistant and homozygous susceptible F4ac pigs did not reveal any causative single nucleotide polymorphism in the four genes. Two silent mutations at the positions 295 (C/T) and 313 (T/C) in B3GALT3 were found. Using the somatic cell hybrid panel, B3GnT5 and B3GALT3 were assigned to the chromosomal region SSC13q23-q41. No mutations were found in the cDNA sequences of these genes associated with the F4acR genotypes. [source] Genetic variances due to imprinted genes in cattleJOURNAL OF ANIMAL BREEDING AND GENETICS, Issue 3 2002TH. ENGELLANDT The effect of paternally expressed, i.e. maternally imprinted, genes on slaughter records from 2744 German Gelbvieh finishing bulls were estimated. Significant effects of paternal gametes were found for two fatness traits and an estimate of meat content. Paternally expressed genes explained 14 and 16% of the phenotypic variances for pelvic fat and kidney fat, respectively. Ignoring paternal gametic effects resulted in inflated estimates of the additive genetic variances. The heritabilities of pelvic and kidney fat dropped from 0.31 to 0.16 and from 0.59 to 0.28, respectively, when paternal gametes were fitted. A 15% influence of paternally expressed genes and a reduction in heritability of 20% were also found for estimated meat content. Simulation studies demonstrated that the uncorrelated random effect of the sire is a useful indicator for the presence of paternal gametic effects in variance component estimations. The presented results correspond well with findings in swine, where a paternally expressed QTL at the Igf2 gene influences similar trait complexes. A viable speculation could therefore be that an imprinted bovine Igf2 gene caused the effects described here. Genetische Variation auf Grund von paternal exprimierten Genen beim Rind In der vorliegenden Studie wurde der Effekt von paternal exprimierten Genen auf Merkmale der Schlachtleistung und des Schlachtkörperwertes von 2744 Mastbullen der Rasse Gelbvieh geschätzt. Signifikante Effekte von paternal exprimierten Genen wurden für zwei Fettabschnitte des Schlachtkörpers und für den geschätzten Fleischanteil gefunden, sie erklärten 14% und 16% der phänotypischen Varianzen des Becken- und Nierenfettes. Das Nichtbeachten dieser Effekte im linearen Schätzmodell führte zu einer Überschätzung der additiv genetischen Varianzen in den jeweiligen Merkmalen. Wurden die Effekte der paternalen Gameten im Schätzmodell berücksichtigt, fielen die geschätzten Heritabilitäten des Becken- und Nierenfettes von h2=0,31 auf h2=0,16 bzw. von h2=0,59 auf h2=0,28. Der Einfluß der paternalen Gameten auf die phänotypische Varianz des geschätzten Fleischanteils des Schlachkörpers wurde auf 15% geschätzt. Bei Berücksichtigung dieser Effekte im linearen Schätzmodell fiel die Heritabilität des Merkmals Fleischanteil um 20%. In Simulationsstudien konnte gezeigt werden, daß der unkorrelierte, zufällige Effekt des Vaters ein nützlicher Indikator für eventuell vorhandene paternal gametische Effekte bei der Schätzung von Varianzkomponenten sein kann. Die gefundenen Ergebnisse weisen Parallelen zu Untersuchungen beim Schwein auf, wo ein paternal exprimierter QTL am Igf2 Locus ähnliche Merkmalskomplexe beeinflußt. Eine mögliche Spekulation könnte daher sein, daß die hier beschriebenen Effekte beim Rind durch einen Igf2 Locus verursacht werden, der dem Phänomen des Imprinting unterliegt. [source] Identification of monozygous twins and microsatellite mutation rate in pigs from QTL linkage analysis dataJOURNAL OF ANIMAL BREEDING AND GENETICS, Issue 5 2001L. Grapes In previous work, microsatellite markers have primarily been tools for genome studies. However, the use of marker data can be extended beyond its original intent to maximize the amount of information obtained. There have been few studies to determine the occurrence of monozygous (MZ) twins in pigs. The advent of DNA marker technology, and microsatellites in particular, allows MZ twins to be identified based on their genotype data. To determine if MZ twin births occur in pigs, genotypes for F2 individuals, n=525, from 65 Berkshire × Yorkshire families were examined. One pair of female twins was found to have matching genotype data (95% CI: 0,2.94 twins). This is a unique result since there have been no published reports to date of twin pigs that survived until birth. Additionally, three dinucleotide microsatellite mutations were found after screening 134 565 meioses of 125 loci spanning the entire genome and the X chromosome. The average mutation rate for the population, n=570, was 2.23 × 10,5 (95% CI: 6.17 × 10,6,6.51 × 10,5). A mutation rate similar to this was published earlier for dinucleotide repeat microsatellite mutations in swine. Identification de jumeaux monozygotes et taux de mutation des microsatellites à partir de données d, analyse de liaison avec des caractères quantitatifs Jusqu'à présent, les marqueurs microsatelittes ont été principalement utilisés comme outils pour étudier le génome. Cependant, l'utilisation des données de marquage peut être étendue au delà de ce but initial et permet d'obtenir d'autres types d'informations. Au cours des dernières années, il n'y a eu que peu d'études visant à determiner l'existence de jumeaux monozygotes (MZ) chez le porc. L'avènement des techniques de marquage de l'ADN, et plus particulièrement des microsatelittes, permet l'identification de jumeaux MZ sur la base de leur génotype aux marqueurs. Afin de déterminer s'il existe des jumeaux MZ chez le porc, nous avons examiné les génotypes d'individus F2 (n=525) issus de 65 familles Berkshire × Yorkshire. La recherche d'individus ayant un genotype identique a permis d'identifer un couple de jumeaux femelles (95% CI: 0,2.94). Il s'agit d'un résultat unique car jusqu'à ce jour, il n'y avait aucun cas publié de jumeaux ayant survécus après la naissance chez le porc. Par ailleurs, après analyse de 134 565 méioses pour 125 loci répartis sur l'ensemble du génome et sur le chromosome X, 3 mutations ont été trouvées au niveau de microsatelittes dinucleotidiques. Le taux moyen de mutation dans la population (n=570) a été estiméà 2.23 × 10,5 (95% IC: 6.17 × 10,6à 6.51 × 10,5). Un taux de mutation similaire à celui-ci a été publié précédemment pour des marqueurs microsatelittes dinucleotidiques chez le porc. [source] Stability of genetic parameter estimates for production traits in pigsJOURNAL OF ANIMAL BREEDING AND GENETICS, Issue 3 2001J. Wolf Changes in variance component estimates in growing sets of performance data in two pig breeds were investigated. Data was used from the field and station test of Czech Landrace (LA: 75 099 observations) and the Slovakian breed, White Meaty swine (WM: 32 203 observations). In LA the traits analysed were estimated lean meat content (LM) and average daily gain (ADGF) on field test and average daily gain (ADGS) and weight of valuable cuts (VCW) on station test. In WM the traits analysed were backfat thickness on field and station test (BFF, BFS, respectively), proportion of valuable cuts (VCP) on station test, ADGF and ADGS. Covariance components were estimated from four- and five-trait animal models using the VCE software. Omitting data from factor levels with a low number of records led to 4.2% of LA records and 21.7% of WM records being deleted. Changes in genetic and residual variance estimates were less than 5% for all traits in LA and less than 12% for all traits except ADGS in WM. The changes in estimated genetic variances caused by 18 months (LA) or 24 months (WM) of new data were 2,25% and the changes in estimated residual variances were less than 5% in LA and less than 20% in WM. In both breeds, changes in heritability estimates did not exceed 0.06 in absolute value. In LA, it is reasonable to use genetic parameter estimates for 3 years before re-estimation. In WM the time interval should be shorter because of changes in the estimates caused by their lower accuracy arising from the smaller size of the data-set and smaller frequency of station testing. Stabilität der Schätzwerte genetischer Parameter für Produktionsmarkmale beim Schwein Für zwei Schweinerassen wurden Änderungen der Varianzkomponentenschätzwerte in wachsenden Leistungsprüfungsdatensätzen untersucht. Die beiden Ausgangsdatensätze bestanden aus Feld- und Stationsprüfdaten der Tschechischen Landrasse (LA , 75 099 Beobachtungen) bzw. der Slowakischen Rasse White Meaty (WM , 32 203 Beobachtungen). Folgende Merkmale wurden ausgewertet: Magerfleischanteil (LM) und Lebenstagszunahme (ADGF) aus der Feldprüfung sowie Prüftagszunahme (ADGS) und Gewicht wertvoller Teilstücke (VCW) aus der Stationsprüfung bei der LA; Rückenspeckdicke aus der Feld- und Stationsprüfung (BFF bzw. BFS), Anteil wertvoller Teilstücke (VCP) aus der Stationsprüfung sowie ADGF und ADGS bei der Rasse WM. Die Kovarianzkomponenten wurden für Vier- bzw. Fünf-Merkmals-Tiermodelle mit dem Programm VCE berechnet. Das Auslassen von Daten von Klassen mit geringer Besetzung führte dazu, daß in der LA 4,2% und in WM 21,7% der Daten gelöscht wurden. Die Änderungen in den genetischen und den Rest-Varianzen waren in der LA bei allen Merkmalen kleiner als 5% und in WM bei allen Merkmalen mit Ausnahme von ADGS kleiner als 12%. Durch Hinzufügen von Daten aus einem Zeitraum von 18 (LA) bzw. 24 (WM) Monaten änderten sich die genetischen Varianzen um 2 bis 25%. Die Änderungen in den Restvarianzen lagen unter 5% bei der LA und unter 20% bei WM. Die maximale Änderung der Heritabilitätskoeffizienten überstieg in beiden Rassen nicht 0,06. Bei der LA sollte ein Zeitintervall von drei Jahren zu einer Neuschätzung der genetischen Parameter ausreichen, bei WM sollte wegen der beobachteten Änderungen der Schätzwerte, der kleineren Datenmenge und des geringeren Anteils stationsgeprüfter Tiere das Zeitintervall kürzer sein. [source] Identification of a Brevibacterium marker gene specific to poultry litter and development of a quantitative PCR assayJOURNAL OF APPLIED MICROBIOLOGY, Issue 1 2010J.L. Weidhaas Abstract Aim:, To identify a DNA sequence specific to a bacterium found in poultry litter that was indicative of faecal contamination by poultry sources. Methods and Results:, Faecally contaminated poultry litter and soils were used as source material for the development of a quantitative polymerase chain reaction (qPCR) method targeting the 16S rRNA gene of a Brevibacterium sp. The identified sequence had 98% nucleotide identity to the 16S rRNA gene of Brevibacterium avium. The qPCR method was tested on 17 soiled litter samples; 40 chicken faecal samples; and 116 nontarget faecal samples from cattle, swine, ducks, geese, and human sewage collected across the United States. The 571-bp product was detected in 76% of poultry-associated samples, but not in 93% of faecal samples from other sources. Marker concentrations were 107,109 gene copies per gram in soiled litter, up to 105 gene copies per gram in spread-site soils, and 107 gene copies per litre in field run-off water. Results were corroborated by a blinded study conducted by a second laboratory. Conclusion:, The poultry-specific PCR product is a useful marker gene for assessing the impact of faecal contamination as a result of land-applied poultry litter. Significance and Impact of the Study:, This study describes the first quantitative, sensitive and specific microbial source tracking method for the detection of poultry litter contamination. [source] Detection and distribution of probiotic Escherichia coli Nissle 1917 clones in swine herds in GermanyJOURNAL OF APPLIED MICROBIOLOGY, Issue 6 2006S. Kleta Abstract Aims:, To verify the presence of Escherichia coli Nissle 1917 as a natural isolate in swine and to characterize in vitro probiotic properties as well as in vivo persistence in a feeding experiment. Methods and Results:, During studies on the intestinal microflora of pigs, we isolated E. coli Nissle 1917 sporadically from a pig population over a period of 1 year. The identity of the isolates as E. coli Nissle 1917 was verified by serotyping, Nissle-specific PCR, macrorestriction analysis (pulsed field gel electrophoresis) and the determination of in vitro probiotic properties in invasion and adhesion assays using a porcine intestinal epithelial cell line. Both the E. coli isolates and the E. coli Nissle 1917 strain showed strong reductions in adhesion of porcine enteropathogenic E. coli and invasion of Salmonella typhimurium with epithelial cells in vitro, with a probiotic effect. Screening of five epidemiologically unlinked swine farms and two wild boar groups showed one farm positive for E. coli Nissle 1917. A feeding experiment with four piglets showed viable E. coli Nissle 1917 in the intestine of three animals. Conclusions:, The results of this study suggest that the E. coli Nissle 1917 strain is already partially established in swine herds, but the colonization of individual animals is variable. Significance and Impact of the Study:, We report natural, long-term colonization and transmission of the probiotic E. coli Nissle 1917 strain in a swine herd, characterized individual persistence and colonization properties in swine and established an in vitro porcine intestinal epithelial cell model of probiotic action. The results of this study would have implications in the use of this strain as a probiotic in swine and contribute to a better understanding of the individual nature of intestinal bacterial persistence and establishment. [source] A porcine model for fixed drug eruptions in humans: the case of antipyrine in the Yucatan micropigJOURNAL OF APPLIED TOXICOLOGY, Issue 1 2006Yvonne Pak Abstract To date, there is no acceptable animal model to investigate fixed drug eruptions (FDEs) in humans. We briefly report here observations suggesting that the Yucatan micropig may be a useful animal model for that purpose. During an investigation of antipyrine absorption and disposition, we observed the development of FDEs after intravenous administration of a 1 g dose. Our observations were consistent with those reported in several investigations of humans taking a single dose of antipyrine. To confirm these results, a naïve micropig was challenged. A male uncastrated Yucatan micropig (27.2 kg) was given a 1 g dose of antipyrine intravenously. After 30 days, this pig was rechallenged with the same intravenous dose of antipyrine (1 g). Blood samples were obtained to examine immunological endpoints. During the initial challenge, a fluid plaque (ca. 1,1.5 cm) appeared on the left hip of the pig ca. 6 h after dosing. After the rechallenge, inflamed pink patches were observed at the same sites where the blisters formed initially; however, no blisters re-formed. Changes of neutrophil, lymphocyte and eosinophil levels from baseline were noted 8 h after challenge. The micropig did not seem otherwise affected by the FDEs. These observations suggest that the Yucatan micropig, or swine in general, may be a useful animal model for detecting drugs that may cause FDEs in humans. Copyright © 2005 John Wiley & Sons, Ltd. [source] Site-specific percutaneous absorption of methyl salicylate and VX in domestic swineJOURNAL OF APPLIED TOXICOLOGY, Issue 3 2002E. J. Scott Duncan Abstract The site specificity of the percutaneous absorption of methyl salicylate (MeS) and the organophosphate nerve agent VX (O -ethyl S -(2-diisopropylaminoethyl) methylphosphonothioate) was examined in anaesthetized domestic swine that were fully instrumented for physiological endpoints. Four different anatomical sites (ear, perineum, inguinal crease and epigastrium) were exposed to the MeS and the serum levels were measured over a 6-h time period. The dose absorbed at the ear region was 11 ,g cm,2 with an initial flux of 0.063 ,g cm,2min,1, whereas at the epigastrium region the dose absorbed was 3 ,g cm,2 with an initial flux of 0.025 ,g cm,2min,1. For this reason further studies were carried out with VX on the ear and the epigastrium only. In animals treated with agent on the epigastrium, blood cholinesterase (ChE) activity began to drop 90 min after application and continued to decline at a constant rate for the remainder of the experiment to ca. 25% of awake control activity. At this time there were negligible signs of poisoning and the medical prognosis was judged to be good. In contrast, the ChE activity in animals receiving VX on the ear decreased to 25% of awake control values within 45 min and levelled out at 5,6% by 120 min. Clinical signs of VX poisoning paralleled the ChE inhibition, progressing in severity over the duration of the exposure. It was judged that these animals would not survive. The dramatic site dependence of agent absorption leading to vastly different toxicological endpoints demonstrated in this model system has important ramifications for chemical protective suit development, threat assessment, medical countermeasures and contamination control protocols. Copyright © 2002 Crown in the right of Canada. Published by John Wiley & Sons, Ltd. [source] Genetically Manipulated Human Skeletal Myoblast Cells for Cardiac TransplantationJOURNAL OF CARDIAC SURGERY, Issue 6 2002Kh H Haider Aim: Considering the promise of skeletal myoblast cell transplantation to improve cardiac function in myocardial myopathies, we aim in the present study to investigate the potential of human skeletal myoblast cells (HSMC) as a carrier for therapeutic genes for the heart muscle. Methods: Skeletal muscle sample is obtained from rectus femoris of the donor and is processed in the tissue culture to generate HSMC by a patented process of Cell Therapy Inc. The HSMC are grown in large 225 mm2 tissue culture flasks coated with collagen for enhanced cell adherence, using patented Super Medium (Cell Therapy Inc., Singapore) containing 10% fetal calf serum, to 80% confluence. The HSMC are passaged at regular time intervals of 48-72 hours to prevent in vitro differentiation. The HSMC thus obtained are transduced three times with retroviral vector carrying Lac-Z reporter gene before transplantation. The Lac-Z transduced HSMC are harvested by trypsinization, washed and re-suspended in serum free Super Medium. Ischemic Porcine model is created by clamping ameroid ring around left circumflex coronary artery in Yorkshire swine, four weeks prior to cell transplantation. For cell transplantation, the animal is anaesthetized, ventilated and heart is exposed by left thoracotomy. Fifteen injections (0.25 ml each) containing 300 million cells are injected in to the left ventricle endocardially under direct vision. For control animal, only culture medium without cells is injected. The animal is euthanized at pre-determined time, heart is explanted and processed for histological examination. The cryosectioning of the tissue and subsequent staining for Lac-Z expression and Hematoxylin-Eosin staining is carried out by standard methods. Results: The skeletal muscle samples processed by the patented method of Cell Therapy yield 85-90% pure HSMC. The preliminary data shows that repeated transductions of myoblast cells with retrovirus carrying Lac-Z yield highly efficient 70-75% Lac-Z positive HSMC population (Figure 1). Dye exclusion test using Trypan blue reveals >95% cell viability at the time of injection. Gross sections of the cardiac tissue stained positive for Lac-Z expression (Figure 2). Histological examination showed the presence of grafted myoblast cells expressing Lac-Z gene in the cardiac tissue (Figure 3). Conclusion: In the light of our preliminary results, we conclude that HSMC may prove to be excellent carriers of transgene for cardiac muscle cells which otherwise are refractory to ordinary gene transfection methods. The use of HSMC mediated gene delivery to cardiac muscle is safer as compared to direct injection of viral vectors in to the heart muscle. Furthermore, the grafted myoblast cells will additionally serve to strengthen the weakened heart muscle. Figure 1.Human Skeletal myoblasts transduced with Lac-Z carrying retrovirus and stained with x-gal. Figure 2.Gross sections of heart muscle stained for Lac-Z expression. Figure 3.X-gal stained porcine heart muscle counter-stained with Eosin. The heart was explanted 6 weeks after transplantation of Lac-Z stained human myoblasts. The arrow shows Lac-Z expressing myoblast cells. [source] Dynamic Registration of Preablation Imaging With a Catheter Geometry to Guide Ablation in a Swine Model: Validation of Image Integration and Assessment of Catheter Navigation AccuracyJOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY, Issue 1 2010J. JASON WEST M.D. Image Integration with a Catheter Mapping System.,Background: Catheter ablation of atrial and ventricular tachyarrhythmia involves anatomically based cardiac ablation strategies. CT and MRI images provide the most detailed cardiac anatomy available. Integration of these images into a mapping system should produce detailed and accurate models suitable to guide ablation. Objective: The purpose of this study was to validate and assess the accuracy of a novel CT and MRI image integration algorithm designed to facilitate catheter navigation and ablation. Methods: Using a lateral thoracotomy, markers were sutured to the epicardial surface of each cardiac chamber in 12 swine. Detailed CT/MRI anatomy was imported into the mapping system. The CT/MRI image was then integrated with a detailed catheter geometry of the relevant chamber using a new image integration algorithm. The epicardial markers, identified from the CT/MRI images, were then displayed on the surface of the integrated image. Guided only by the integrated CT/MRI, a single RF lesion was directed at the corresponding endocardial site for each epicardial marker. At autopsy, the distance from the endocardial RF lesion to the target site was assessed. Results: The mean position error (CT/MRI) for the left atrium was 2.5 ± 2.4 mm/5.1 ± 3.9 mm, for the right atrium 6.2 ± 6.5 mm/4.3 ± 2.2 mm, for the right ventricle 6.2 ± 4.3 mm/6.6 ± 5.3 mm, and for the left ventricle 4.7 ± 3.4 mm/3.1 ± 2.7 mm. There was no cardiac perforation or tamponade. Conclusion: CT and MRI images can be effectively utilized for catheter navigation when integrated into a mapping system. This novel registration module with dynamic registration provides effective guidance for ablation. (J Cardiovasc Electrophysiol, Vol. 21, pp. 81,87, January 2010) [source] | |||||||||||||||||||||||||||||||||||||