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Selected AbstractsP28 Interleukin-8 from keratinocytes can be used to test for contact allergyCONTACT DERMATITIS, Issue 3 2004Bolli Bjarnason Objective:, To investigate whether secretion of interleukin-8 (IL-8) proteins by keratinocytes following in vitro exposure to a contact allergen can be used to detect contact allergy. Methods:, Suction blisters were made on skin of allergic and anergic subjects to urushiol, the contact allergen of poison ivy. Keratinocyte cultures were prepared and exposed to the allergen in vitro. Controls were the allergen solvent. Variable allergen concentrations, allergen exposure times and cell culture times were used. At the end of each culture time, IL-8 RNA and protein of the culture supernatants were analyzed by PCR and ELISA. Results:, The concentration of IL-8 in the supernatants proved to be a successful way to distinguish between subjects who patch tested positive with a non-toxic concentration of urushiol and subjects who tested negative. In the allergic subjects, a correlation was established between the dose of the allergen and the IL-8 protein concentration in the supernatants. Conclusions:, In vitro testing of contact allergies in patients makes possible an objective assessment of their allergic status without causing a booster effect or risking active sensitizations. The results indicate that the method may be used as an alternative method to animal models for testing consumer products before their marketing, thus avoiding ethical problems and problems related to interpretation of tests because of biological differences between animals and humans. [source] Bariatric surgery , a successful way to battle the weight crisisBRITISH JOURNAL OF SURGERY (NOW INCLUDES EUROPEAN JOURNAL OF SURGERY), Issue 3 2006M. Weber No abstract is available for this article. [source] Developing a Resilience Package for Vulnerable ChildrenCHILD AND ADOLESCENT MENTAL HEALTH, Issue 4 2002Maurice Place Background: In recent years great efforts have been made to clarify what factors make it more likely that a child will develop mental health problems, and what elements increase the resilience of vulnerable children, and hence protect them from becoming disturbed. With the increase in understanding about the nature of such resilience comes the possibility of developing preventative packages for at-risk groups. Method: Children whose parents have significant depressive illness have been offered a supportive package that seeks to increase their resilience. This focuses on the child's resources, family education and interaction, and community resources and supports for the family to access. Results: Initial results show the package increases social activity, reduces emotionality and helps families become more flexible. Conclusion: Helping children reduce their risk of developing mental health problems is rightly an area of growing importance. This package is a potentially successful way of achieving this by promoting their resilience. [source] Affinity prefractionation for MS-based plasma proteomicsPROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 6 2009Maria Pernemalm Abstract The plasma proteome has proven to be one of the most challenging proteomes to profile using currently available proteomics technologies. A plethora of methodologies have been used to profile human plasma in order to discover potential biomarkers for disease and for therapy optimization. Affinity-based prefractionation coupled to MS has been shown to be one of the most successful ways to dig deeper into the plasma proteome. Depletion of high abundant plasma proteins is becoming an initial method of choice in any plasma profiling project. However, several other affinity-based enrichment methods have been published in recent years. Here we review both protein and peptide affinity prefractionation methods coupled with MS-based proteomics. Analysis of the proportion of cellular and extracellular annotated proteins of publicly available MS plasma proteomics data is performed to estimate the analytical depth of various prefractionation methods. [source] | ||||||||||