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Subsequent Regeneration (subsequent + regeneration)
Selected AbstractsUltrastructural changes in skeletal muscle of the tail of the lizard Hemidactylus mabouia immediately following autotomyACTA ZOOLOGICA, Issue 4 2010Tomaz Henrique Araújo Abstract Araújo, T.H., Faria, F.P., Katchburian, E. and Freymüller, E. (2009). Ultrastructural changes in skeletal muscle of the tail of the lizard Hemidactylus mabouia immediately following autotomy. ,Acta Zoologica (Stockholm) 91: 440,446. Although autotomy and subsequent regeneration of lizard tails has been extensively studied, there is little information available on ultrastructural changes that occur to the muscle fibers at the site of severance. Thus, in the present study, we examine the ultrastructure of the musculature of the remaining tail stump of the lizard Hemidactylus mabouia immediately after autotomy. Our results show that exposed portions of the skeletal muscle fibers of the stump that are unprotected by connective tissue bulge to produce large mushroom-like protrusions. These exposed portions show abnormal structure but suffer no leakage of cytoplasmic contents. Many small and large vesicular structures appeared between myofibrils in the interface at this disarranged region (distal) and the other portion of the fibers that remain unchanged (proximal). These vesicles coalesce, creating a gap that leads to the release of the mushroom-like protrusion. So, our results showed that after the macroscopic act of autotomy the muscular fibers release part of the sarcoplasm as if a second and microscopic set of autotomic events takes place immediately following the macroscopic act of autotomy. Presumably these changes pave the way for the formation of a blastema and the beginning of regeneration. [source] Effects of forest harvesting on the occurrence of landslides and debris flows in steep terrain of central JapanEARTH SURFACE PROCESSES AND LANDFORMS, Issue 6 2008Fumitoshi Imaizumi Abstract Landslides and debris flows associated with forest harvesting can cause much destruction and the influence of the timing of harvesting on these mass wasting processes therefore needs to be assessed in order to protect aquatic ecosystems and develop improved strategies for disaster prevention. We examined the effects of forest harvesting on the frequency of landslides and debris flows in the Sanko catchment (central Japan) using nine aerial photo periods covering 1964 to 2003. These photographs showed a mosaic of different forest ages attributable to the rotational management in this area since 1912. Geology and slope gradient are rather uniformly distributed in the Sanko catchment, facilitating assessment of forest harvesting effects on mass wasting without complication of other factors. Trends of new landslides and debris flows correspond to changes in slope stability explained by root strength decay and recovery; the direct impact of clearcutting on landslide occurrence was greatest in forest stands that were clearcut 1 to 10 yr earlier with progressively lesser impacts continuing up to 25 yr after harvesting. Sediment supply rate from landslides in forests clearcut 1 to 10 yr earlier was about 10-fold higher than in control sites. Total landslide volume in forest stands clearcut 0 to 25 yr earlier was 5·8 × 103 m3 km,2 compared with 1·3 × 103 m3 km,2 in clearcuts >25 yr, indicating a fourfold increase compared with control sites during the period when harvesting affected slope stability. Because landslide scars continue to produce sediment after initial failure, sediment supply from landslides continues for 45 yr in the Sanko catchment. To estimate the effect of forest harvesting and subsequent regeneration on the occurrence of mass wasting in other regions, changes in root strength caused by decay and recovery of roots should be investigated for various species and environmental conditions. Copyright © 2007 John Wiley & Sons, Ltd. [source] Factors affecting adventitious regeneration from in vitro leaf explants of ,Improved French' plum, the most important dried plum cultivar in the USAANNALS OF APPLIED BIOLOGY, Issue 1 2010C. Petri An adventitious shoot regeneration protocol from in vitro leaves of the most important dried plum cultivar in the USA, ,Improved French', has been established. Factors affecting regeneration were studied in order to optimise regeneration. The proliferation medium in which the shoots, used as the source of leaf explants, were cultured had a strong influence on subsequent regeneration. Shoot regeneration was observed at a mean frequency of 52% when a Murashige-based and Skoog-based shoot culture medium with 3 ,M N6 -benzylaminopurine and 0.25 ,M indole-3-butyric acid (IBA) was employed compared with shoot regeneration frequencies of less than 5% for a Quoirin-based and Lepoivre-based shoot culture medium, with 8.9 ,M N6 -benzylaminopurine and 0.49 ,M IBA. The shoot regeneration medium contained ,-naphthaleneacetic acid at 2.0,6.0 ,M and thidiazuron at 4.5,15.0 ,M. 2,4 Dichlorophenoxy-acetic acid at 9.0 ,M was included in the medium but only for the first 4 days of culture. Shoot regeneration frequencies were positively related to thidiazuron concentration and significantly greater (P < 0.05) for 9,15 ,M thidiazuron than for the media with 4.5 ,M thidiazuron. Leaf explants, incubated in a 16-h-light/8-h-dark photoperiod or in the dark for 1 week followed by exposure to light, showed significantly more organogenic activity (P < 0.01) than was observed for leaves cultured in the dark for 2 or 3 weeks before they were transferred to the light. The utilisation of Bacto agar (0.7%) as the gelling agent increased organogenesis compared with media gelled with TC Agar (0.7%), or an agar,gellan gum blend (AgargelÔ) (0.45%). The addition of the ethylene inhibitor silver thiosulphate at 60,120 ,M also improved organogenesis. When all the studied factors were optimised, a regeneration rate of 65% was achieved. Rooting frequency of regenerated shoots was significantly increased (P < 0.05) by the use of full-strength Murashige and Skoog salts (40%) or 100 mg L,1 phloroglucinol (53%) to the rooting medium. [source] Spatial ecology of a threatened python (Morelia spilota imbricata) and the effects of anthropogenic habitat changeAUSTRAL ECOLOGY, Issue 3 2005D. PEARSON Abstract Large predators play important ecological roles, but often are sensitive to habitat changes and thus are early casualties of habitat perturbation. Pythons are among the largest predators in many Australian environments, and hence warrant conservation-orientated research. Carpet pythons (Morelia spilota imbricata) have declined across much of south-western Australia presumably because of habitat clearance and degradation. Information on habitat use, home range sizes and movements is needed to plan for the conservation of this important predator. We studied pythons at two study sites (Garden Island and Dryandra Woodland) with markedly different climates, habitat types and disturbance histories. We surgically implanted radio-transmitters in 91 pythons and tracked them for periods of 1 month to 4 years. Dryandra pythons remained inactive inside tree hollows during cooler months (May,September), whereas some (especially small) pythons on Garden Island continued to move and feed. Overall weekly displacements (mean = 100,150 m) were similar at the two study sites and among sex/age classes, except that reproductive females were sedentary during summer while they were incubating eggs. Home ranges averaged 15,20 ha. Adult male pythons had larger home ranges than adult females at Dryandra, but not at Garden Island. Radio-tracked snakes at Dryandra exhibited high site fidelity, returning to previously occupied logs after long absences and reusing tree hollows for winter shelter. Many of the logs used by snakes had been felled during plantation establishment >70 years ago, with little subsequent regeneration of source trees. In contrast, Garden Island snakes usually sheltered under dense shrubs. Habitat usage was similar among different sex/age classes of snakes at each site, except that juvenile pythons were more arboreal than adults. Although carpet pythons demonstrate great flexibility in habitat use, certain habitat elements appear critical for the persistence of viable populations. Fire plays a central role in this process, albeit in complex ways. For example, low-intensity fires reduce the availability of hollow logs on the ground at Dryandra and fail to regenerate shrub thickets required by prey species. Paradoxically, high-intensity fires stimulate shrub thickets and fell trees creating new logs , but might also threaten overwinter trees. Thus, the impact of disturbances (such as wildfires) on the viability of python populations will be mediated in complex ways by alteration to important microhabitats such as vegetation cover or log availability. At Dryandra, landscape management should include occasional fire events to generate new logs as well as shrub thickets used by prey. Strategic burning may also be required at Garden Island to regenerate some vegetation communities. [source] | ||||||||||