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Step Approach (step + approach)

Distribution by Scientific Domains

Chemistry	40%

Selected Abstracts

In Situ Bioconjugation: Single Step Approach to Tailored Nanoparticle-Bioconjugates by Ultrashort Pulsed Laser Ablation

ADVANCED FUNCTIONAL MATERIALS, Issue 8 2009
Svea Petersen
Abstract A single step approach to tailored nanoparticle-bioconjugates, enabling the generation of gold nanoparticles by laser ablation and their in situ conjugation with any biomolecule bearing an electron donating function (e.g., thiolated oligonucleotides) is established. The integrity of oligonucleotides after conjugation and the stability of bioconjugates in physiological media are investigated. Their size is tailorable via process parameters. This rapid and universal method may provide biochemists with various nanoparticle-bioconjugates for screening the often unpredictable structure,function relationship. [source]

Proton Catalyzed Amino-Claisen Rearrangement: A Practical One Step Approach to the Synthesis of 2-Allylarylamines from N-Allylarylamines.

CHEMINFORM, Issue 19 2007
Seema Jain
Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF. [source]

In Situ Bioconjugation: Single Step Approach to Tailored Nanoparticle-Bioconjugates by Ultrashort Pulsed Laser Ablation

The Treatment for Postirradiation Otitis Media With Effusion: A Study of Three Methods

THE LARYNGOSCOPE, Issue 11 2008
Yao-Dong Xu MD
Abstract Objective: To explore treatments for postirradiation otitis media with effusion (OME) in patients with nasopharyngeal carcinoma. Study Design: This study is a prospective quasi-randomized clinical trial. Methods: Ninety-six patients (135 ears) with OME after the first course of radiotherapy for nasopharyngeal carcinoma were divided into three groups: simple auripuncture plus aspiration, tympanic membrane fenestration with cauterization, and myringotomy plus grommet insertion. Cure rates and incidences of complications were compared. Results: Two deaths occurred. The other 94 patients (132 ears) finished a 2-year follow-up. In group 1, four ears (8.9%) were cured after the first treatment and 17 ears overall (37.8%) were cured by the end of the follow-up. Twenty ears (44.4%) had persistent fluid, two ears (4.4%) developed chronic suppurative otitis media, and five ears (11.1%) developed dry eardrum perforation. In group 2, seven ears (15.6%) were cured after the first treatment and 21 ears overall (46.7%) were cured by the end of the follow-up. Fourteen ears (31.1%) had persistent fluid, three ears (6.7%) developed chronic suppurative otitis media, and seven ears (15.6%) developed dry eardrum perforation. In group 3, eight ears (17.8%) were cured after the first treatment and 23 ears overall (51.1%) were cured by the end of the follow-up. Seven ears (15.6%) had persistent fluid, five ears (11.1%) developed chronic suppurative otitis media, three ears (6.7%) developed eardrum perforation with effusion, and five ears (11.1%) developed dry eardrum perforation. Conclusion: The methods each have advantages and disadvantages. We believe that a step by step approach should be used when choosing the treatment method for postirradiation OME. That is, first apply auripuncture plus aspiration, and then the other methods if this approach is inadequate. Enhanced local care after grommet insertion can effectively reduce the incidence of complications. [source]

New approach for rapid detection of known hemoglobin variants using LC-MS/MS combined with a peptide database,

JOURNAL OF MASS SPECTROMETRY (INCORP BIOLOGICAL MASS SPECTROMETRY), Issue 3 2007
F. Basilico
Abstract The identification of hemoglobin (Hb) variants is usually performed by means of different analytical steps and methodologies. Phenotypic methods, such as gel electrophoresis and high performance liquid chromatography, are used to detect the different electrophoretic or chromatographic behaviors of hemoglobin variants in comparison to HbA0 used as a control. These data often need to be combined with mass spectrometry analyses of intact globins and their tryptic peptide mixtures. As an alternative to a ,step-by-step' procedure, we have developed a ,single step' approach for the identification of Hb variants present in biological samples. This is based on the µHPLC-ESI-MS/MS analysis of the peptide mixture generated by a tryptic digestion of diluted Hb samples and an in-house new database containing solely the variant tryptic peptide of known human Hb variants. The experimental results (full MS and MS/MS spectra) are correlated with theoretical mass spectra generated from our in-house-built variant peptide database (Hbp) using the SEQUEST algorithm. Simple preparation of samples and an automated identification of the variant peptide are the main characteristics of this approach, making it an attractive method for the detection of Hb variants at the routine clinical level. We have analyzed 16 different samples, each containing a different known variant of hemoglobin. Copyright © 2006 John Wiley & Sons, Ltd. [source]