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Stark Contrast (stark + contrast)
Selected AbstractsPrivatisation and outsourcing in wartime: the humanitarian challengesDISASTERS, Issue 4 2006Gilles Carbonnier Abstract The tendency today to privatise many activities hitherto considered the exclusive preserve of the state has given rise to sharp debate. The specific nature of humanitarian emergencies elucidates in particularly stark contrast some of the main challenges connected to the privatisation and outsourcing of essential public services, such as the provision of drinking water and health care. Privatising the realms of defence and security, which are at the very core of state prerogative, raises several legal and humanitarian concerns. This article focuses on the roles and responsibilities of the various parties involved in armed conflicts, especially those of private companies engaged in security, intelligence and interrogation work, and in the provision of water supply and health services. It highlights the need for humanitarian and development actors to grasp better the potential risks and opportunities related to privatisation and outsourcing with a view to supplying effective protection and assistance to communities affected by war. [source] The underestimated importance of belowground carbon input for forest soil animal food websECOLOGY LETTERS, Issue 8 2007Melanie M. Pollierer Abstract The present study investigated the relative importance of leaf and root carbon input for soil invertebrates. Experimental plots were established at the Swiss Canopy Crane (SCC) site where the forest canopy was enriched with 13C depleted CO2 at a target CO2 concentration of c. 540 p.p.m. We exchanged litter between labelled and unlabelled areas resulting in four treatments: (i) leaf litter and roots labelled, (ii) only leaf litter labelled, (iii) only roots labelled and (iv) unlabelled controls. In plots with only 13C-labelled roots most of the soil invertebrates studied were significantly depleted in 13C, e.g. earthworms, chilopods, gastropods, diplurans, collembolans, mites and isopods, indicating that these taxa predominantly obtain their carbon from belowground input. In plots with only 13C-labelled leaf litter only three taxa, including, e.g. juvenile Glomeris spp. (Diplopoda), were significantly depleted in 13C suggesting that the majority of soil invertebrates obtain its carbon from roots. This is in stark contrast to the view that decomposer food webs are based on litter input from aboveground. [source] Intralesional bovine papillomavirus DNA loads reflect severity of equine sarcoid diseaseEQUINE VETERINARY JOURNAL, Issue 4 2010R. HARALAMBUS Summary Reasons for performing study: Sarcoids are nonmetastasising, yet locally aggressive skin tumours that constitute the most frequent neoplasm in equids. Infection by bovine papillomaviruses types 1 and 2 (BPV-1, BPV-2) has been recognised as major causative factor in sarcoid pathogenesis, but a possible correlation of intralesional virus load with disease severity has not been established thus far. Hypothesis: Given the pathogenic role of BPV-1 and BPV-2 in sarcoid disease, we suggest that intralesional viral DNA concentration may reflect the degree of affection. Methods: Severity of disease was addressed by recording the tumour growth kinetics, lesion number and tumour type for 37 sarcoid-bearing horses and one donkey. Viral load was estimated via quantitative real-time PCR (qPCR) of the E2, E5, L1 and L2 genes from the BPV-1/-2 genome for one randomly selected lesion per horse and correlated with disease severity. Results: Quantitative PCR against E2 identified viral DNA concentrations ranging from 0,556 copies/tumour cell. Of 16 horses affected by quiescent, slowly growing single tumours or multiple mild-type lesions, 15 showed a viral load up to 1.4 copies per cell. In stark contrast, all equids (22/22) bearing rapidly growing and/or multiple aggressive sarcoids had a viral load between 3 and 569 copies per cell. Consistent results were obtained with qPCR against E5, L1 and L2. Conclusions: While tumours of the same clinical type carried variable virus load, confirming that viral titre does not determine clinical appearance, we identified a highly significant correlation between intralesional viral load and disease severity. Potential relevance: The rapid determination of BPV viral load will give a reliable marker for disease severity and may also be considered when establishing a therapeutic strategy. [source] Do ,Quiet' Places Make Animals Placid?ETHOLOGY, Issue 6 2005Island vs. Most animals that possess potent venom display a wide variety of warning messages to discourage predators. Tiger snakes are large and highly venomous elapids that exhibit these anti-predator behaviours. We compared the anti-predator behaviours of two neighbouring and genetically indistinguishable populations in Western Australia (Herdsman Lake, HL and Carnac Island, CI). CI is free from human, native and feral predation. All of these factors represent a continual threat on HL situated on the mainland. Neither body size, nor sex influenced defensive behaviours. However, we observed a marked inter-population difference among adults in the degree to which anti-predator behaviours were displayed when snakes were continually aggravated: HL snakes exhibited a typical warning signal (flat-neck) and bite, while CI snakes remained very docile. In stark contrast, neonates of both populations exhibited marked anti-predator behaviours and both populations were indistinguishable in terms of the intensity of display. Neonates reared in captivity, hence regularly confronted by human predators, became more defensive in comparison with neonates exposed to natural conditions on CI; similarly several adult CI snakes kept in captivity became more defensive. Our results highlight the extreme behavioural plasticity of snakes. We also hypothesize that CI snakes may become more placid over time as they grow up in an environment free from predation. [source] Conformational Flexibility in a Carbobicyclic Diphosphinite LigandEUROPEAN JOURNAL OF INORGANIC CHEMISTRY, Issue 20 2007Ian J. S. Fairlamb Abstract An unsymmetrical bicyclo[3.2.0]heptanyl diphosphinite ligand, FLEXIphosO, shows flexible coordination modes to palladium centres. The X-ray crystal structure for [Pd02(P,P,)3] has been determined which reveals that the bicyclic backbone of the FLEXIphosO ligand exists in an exo -envelope conformation. The change in conformation stands in stark contrast to that observed in mononuclear neutral and cationic palladium(II) complexes containing the FLEXIphosO ligand, where an endo -envelope is observed in solution and in the solid-state. Theoretical studies provide an insight into the relative stability of palladium(0) complexes containing the FLEXIphosO ligand. Another large spanning angle ligand, SPANphos, does not form a similar palladium(0) dimer complex under identical reaction conditions, highlighting the unusual behaviour of the FLEXIphosO ligand.(© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007) [source] Role of the GLT-1 subtype of glutamate transporter in glutamate homeostasis: the GLT-1-preferring inhibitor WAY-855 produces marginal neurotoxicity in the rat hippocampusEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 12 2005Julie V. Selkirk Abstract Glutamate is the major excitatory neurotransmitter in the central nervous system and is tightly regulated by cell surface transporters to avoid increases in concentration and associated neurotoxicity. Selective blockers of glutamate transporter subtypes are sparse and so knock-out animals and antisense techniques have been used to study their specific roles. Here we used WAY-855, a GLT-1-preferring blocker, to assess the role of GLT-1 in rat hippocampus. GLT-1 was the most abundant transporter in the hippocampus at the mRNA level. According to [3H]- l -glutamate uptake data, GLT-1 was responsible for approximately 80% of the GLAST-, GLT-1-, and EAAC1-mediated uptake that occurs within dissociated hippocampal tissue, yet when this transporter was preferentially blocked for 120 h with WAY-855 (100 µm), no significant neurotoxicity was observed in hippocampal slices. This is in stark contrast to results obtained with TBOA, a broad-spectrum transport blocker, which, at concentrations that caused a similar inhibition of glutamate uptake (10 and 30 µm), caused substantial neuronal death when exposed to the slices for 24 h or longer. Likewise, WAY-855, did not significantly exacerbate neurotoxicity associated with simulated ischemia, whereas TBOA did. Finally, intrahippocampal microinjection of WAY-855 (200 and 300 nmol) in vivo resulted in marginal damage compared with TBOA (20 and 200 nmol), which killed the majority of both CA1,4 pyramidal cells and dentate gyrus granule cells. These results indicate that selective inhibition of GLT-1 is insufficient to provoke glutamate build-up, leading to NMDA receptor-mediated neurotoxic effects, and suggest a prominent role of GLAST and/or EAAC1 in extracellular glutamate maintenance. [source] ADAPTIVE POPULATION DIFFERENTIATION IN PHENOLOGY ACROSS A LATITUDINAL GRADIENT IN EUROPEAN ASPEN (POPULUS TREMULA, L.): A COMPARISON OF NEUTRAL MARKERS, CANDIDATE GENES AND PHENOTYPIC TRAITSEVOLUTION, Issue 12 2007David Hall A correct timing of growth cessation and dormancy induction represents a critical ecological and evolutionary trade-off between survival and growth in most forest trees (Rehfeldt et al. 1999; Horvath et al. 2003; Howe et al. 2003). We have studied the deciduous tree European Aspen (Populus tremula) across a latitudinal gradient and compared genetic differentiation in phenology traits with molecular markers. Trees from 12 different areas covering 10 latitudinal degrees were cloned and planted in two common gardens. Several phenology traits showed strong genetic differentiation and clinal variation across the latitudinal gradient, with QST values generally exceeding 0.5. This is in stark contrast to genetic differentiation at several classes of genetic markers (18 neutral SSRs, 7 SSRs located close to phenology candidate genes and 50 SNPs from five phenology candidate genes) that all showed FST values around 0.015. We thus find strong evidence for adaptive divergence in phenology traits across the latitudinal gradient. However, the strong population structure seen at the quantitative traits is not reflected in underlying candidate genes. This result fit theoretical expectations that suggest that genetic differentiation at candidate loci is better described by FST at neutral loci rather than by QST at the quantitative traits themselves. [source] Multiple forms of genetic instability within a 2-Mb chromosomal segment of 3q26.3,q27 are associated with development of esophageal adenocarcinomaGENES, CHROMOSOMES AND CANCER, Issue 4 2006Lin Lin Gene amplification is one of the mechanisms to activate oncogenes in many cancers, including esophageal adenocarcinoma (EA). In the present study, we used two-dimensional restriction landmark genome scanning to clone a NotI/DpnII fragment that showed increased genomic dosage in 1 of 44 EAs analyzed. This fragment maps to 3q26.3,q27, and subsequent experiments identified two intrachromosomal amplicons within a 10-Mb DNA segment in 7 of 75 (9%) EAs. The distal amplified-core region maps centromeric to the PIK3CA locus, and a microsatellite (D3S1754) within this region exhibited significant instability (MSI), in stark contrast to the genomewide microsatellite stability found in EA. D3S1754-MSI arises in premalignant Barrett's dysplastic cells and preceded amplification of the nascent MSI allele in the corresponding EA. Seven ESTs within the amplified-core were overexpressed in amplicon-containing EAs. One of these, EST AW513672, represents a chimeric transcript that initiated from an antisense promoter sequence in the 5,UTR of a full-length LINE-1 element (L1-5,ASP). Similar chimeric transcripts encoding portions of the MET oncogene and the BCAS3 gene also were overexpressed in EAs, suggesting that L1-5,ASP activation may occur at a broad level in primary EAs. Thus, the fine dissection of a 2-Mb amplified DNA segment in 3q26.3,q27 in EA revealed multiple genetic alterations that had occurred sequentially and/or concurrently during EA development. This article has supplementary material, available at http://www.interscience.wiley.com/jpages/1045-2257/suppmat. © 2005 Wiley-Liss, Inc. [source] Oligophenylenevinylenes in Spatially Confined Nanochannels: Monitoring Intermolecular Interactions by UV/Vis and Raman Spectroscopy,ADVANCED FUNCTIONAL MATERIALS, Issue 6 2008Mariya Aloshyna Abstract Perhydrotriphenylene-based channel-forming inclusion compounds (ICs) and thin films made of polyphenylenevinylene (PPV)-type oligomers with terminal alkoxy groups are investigated and compared in a combined experimental and theoretical approach. Interchromophore interactions and host-guest interactions are elucidated by UV/Vis and Raman spectroscopy. The impact of the local environment of the chromophore on the optical and photophysical properties is discussed in light of quantum-chemical calculations. In stark contrast to thin films where preferential side-by-side orientation leads to quenching of photoluminescence (PL) via non-emissive traps, the ICs are found to be attractive materials for opto-electronic applications: they offer high chromophore concentrations, but at the same time behave as quasi-isolated entities of tightly packed, well-oriented objects with high PL quantum yields and the possibility of color tuning. [source] Immigrant Communities and Civil War*INTERNATIONAL MIGRATION REVIEW, Issue 1 2009David D. Laitin This paper explains why international migrants, who face numerous security and cultural threats in their host societies, are almost never implicated in civil war violence. This is quite different from situations of internal migration, which often set off violence that escalates to civil war proportions. The paper first lays out the stark contrast between the political implications of external and internal migration based on data adapted from the Minorities at Risk (MAR) dataset. It then explores the reasons for the low incidence of civil war violence for international migrants through an examination of three cases: Bahrain, which has a large expatriate community without political rights that has been politically quiescent; Estonia, where some 30 percent of the population are disaffected Russian-speakers linked to post-World War II migrations from other republics of the Soviet Union; and Pakistan, where the immigrant Muhajirs are a partial exception to the general pattern outlined in this paper. It concludes with a general statement of the relationship between immigration and rebellion, where the level of grievances is less consequential than the conditions that make insurgency pay off. [source] Price Dispersion and Consumer Reservation PricesJOURNAL OF ECONOMICS & MANAGEMENT STRATEGY, Issue 1 2005Simon P. Anderson We describe firm pricing when consumers follow simple reservation price rules. In stark contrast to other models in the literature, this approach yields price dispersion in pure strategies even when firms have the same marginal costs. At the equilibrium, lower price firms earn higher profits. The range of price dispersion increases with the number of firms: the highest price is the monopoly price, while the lowest price tends to marginal cost. The average transaction price remains substantially above marginal cost even with many firms. The equilibrium pricing pattern is the same when prices are chosen sequentially. [source] Genomic islands of speciation or genomic islands and speciation?MOLECULAR ECOLOGY, Issue 5 2010THOMAS L. TURNER Populations of the malaria mosquito, Anopheles gambiae, are comprised of at least two reproductively isolated, sympatric populations. In this issue, White et al. (2010) use extensive sampling, high-density tiling microarrays, and an updated reference genome to clarify and expand our knowledge of genomic differentiation between these populations. It is now clear that DNA near the centromeres of all three chromosomes are in near-perfect disequilibrium with each other. This is in stark contrast to the remaining 97% of the assembled genome, where fixed differences between populations have not been found, and many polymorphisms are shared. This pattern, coupled with direct evidence of hybridization in nature, supports models of "mosaic" speciation, where ongoing hybridization homogenizes variation in most of the genome while loci under strong selection remain in disequilibrium with each other. However, unambiguously demonstrating that selection maintains the association of these pericentric "speciation islands" in the face of gene flow is difficult. Low recombination at all three loci complicates the issue, and increases the probability that selection unrelated to the speciation process alters patterns of variation in these loci. Here, we discuss these different scenarios in light of this new data. [source] Cryobanking of viable biomaterials: implementation of new strategies for conservation purposesMOLECULAR ECOLOGY, Issue 6 2009DOMINIK LERMEN Abstract Cryobanking, the freezing of biological specimens to maintain their integrity for a variety of anticipated and unanticipated uses, offers unique opportunities to advance the basic knowledge of biological systems and their evolution. Notably, cryobanking provides a crucial opportunity to support conservation efforts for endangered species. Historically, cryobanking has been developed mostly in response to human economic and medical needs , these needs must now be extended to biodiversity conservation. Reproduction technologies utilizing cryobanked gametes, embryos and somatic cells are already vital components of endangered species recovery efforts. Advances in modern biological research (e.g. stem cell research, genomics and proteomics) are already drawing heavily on cryobanked specimens, and future needs are anticipated to be immense. The challenges of developing and applying cryobanking for a broader diversity of species were addressed at an international conference held at Trier University (Germany) in June 2008. However, the magnitude of the potential benefits of cryobanking stood in stark contrast to the lack of substantial resources available for this area of strategic interest for biological science , and society at large. The meeting at Trier established a foundation for a strong global incentive to cryobank threatened species. The establishment of an Amphibian Ark cryobanking programme offers the first opportunity for global cooperation to achieve the cryobanking of the threatened species from an entire vertebrate class. [source] The mating game: do opposites really attract?MOLECULAR ECOLOGY, Issue 6 2008JENNIFER L. GOW When selecting a mate, females of many species face a complicated decision: choosing a very closely related mate will lead to inbreeding, while choosing a mate who is too genetically dissimilar risks breaking up beneficial gene complexes or local genetic adaptations. To ensure the best genetic quality of their offspring, the perfect compromise lies somewhere in between: an optimally genetically dissimilar partner. Empirical evidence demonstrating female preference for genetically dissimilar mates is proof of the adage ,opposites attract'. In stark contrast, Chandler & Zamudio (2008) show in this issue of Molecular Ecology that female spotted salamanders often choose males that are genetically more similar to themselves (although not if the males are small). Along with other recent work, these field studies highlight the broad spectrum of options available to females with respect to relatedness in their choice of mate that belies this rule of thumb. [source] A broad transition zone between an inner Baltic hybrid swarm and a pure North Sea subspecies of Macoma balthica (Mollusca, Bivalvia)MOLECULAR ECOLOGY, Issue 6 2008RAISA NIKULA Abstract The populations of the bivalve clam Macoma balthica in the low-salinity Northern Baltic Sea represent an admixture of two strongly diverged genomic origins, the Pacific Macoma balthica balthica (approx. 60% genomic contribution) and Atlantic Macoma balthica rubra (40%). Using allozyme and mtDNA characters, we describe the broad transition from this hybrid swarm to the pure M. b. rubra in the saline North Sea waters, spanning hundreds of kilometre distance. The zone is centred in the strong salinity gradient of the narrow Öresund strait and in the adjacent Western Baltic. Yet the multilocus clines show no simple and smoothly monotonic gradation: they involve local reversals and strong differences between neighbouring populations. The transitions in different characters are not strictly coincident, and the extent of introgression varies among loci. The Atlantic influence extends further into the Baltic in samples from the southern and eastern Baltic coasts than on the western coast, and further in deeper bottoms than at shallow (< 1 m) sites. This fits with the counterclockwise net circulation pattern and with a presumably weaker salinity barrier for invading Atlantic type larvae in saline deeper water, and corresponding facilitation of outwards drift of Baltic larvae in diluted surface waters. Genotypic disequilibria were strong particularly in the shallow-water samples of the steepest transition zone. This suggests larval mixing from different sources and limited interbreeding in that area, which makes a stark contrast to the evidence of thorough amalgamation of the distinct genomic origins in the inner Baltic hybrid swarm of equilibrium structure. [source] TBP domain symmetry in basal and activated archaeal transcriptionMOLECULAR MICROBIOLOGY, Issue 1 2009Mohamed Ouhammouch Summary The TATA box binding protein (TBP) is the platform for assembly of archaeal and eukaryotic transcription preinitiation complexes. Ancestral gene duplication and fusion events have produced the saddle-shaped TBP molecule, with its two direct-repeat subdomains and pseudo-two-fold symmetry. Collectively, eukaryotic TBPs have diverged from their present-day archaeal counterparts, which remain highly symmetrical. The similarity of the N- and C-halves of archaeal TBPs is especially pronounced in the Methanococcales and Thermoplasmatales, including complete conservation of their N- and C-terminal stirrups; along with helix H,1, the C-terminal stirrup of TBP forms the main interface with TFB/TFIIB. Here, we show that, in stark contrast to its eukaryotic counterparts, multiple substitutions in the C-terminal stirrup of Methanocaldococcus jannaschii (Mja) TBP do not completely abrogate basal transcription. Using DNA affinity cleavage, we show that, by assembling TFB through its conserved N-terminal stirrup, Mja TBP is in effect ambidextrous with regard to basal transcription. In contrast, substitutions in either its N- or the C-terminal stirrup abrogate activated transcription in response to the Lrp-family transcriptional activator Ptr2. [source] Non-growing Escherichia coli cells starved for glucose or phosphate use different mechanisms to survive oxidative stress,MOLECULAR MICROBIOLOGY, Issue 4 2001Patrice L. Moreau Recent data suggest that superoxide dismutases are important in preventing lethal oxidative damage of proteins in Escherichia coli cells incubated under aerobic, carbon starvation conditions. Here, we show that the alkylhydroperoxide reductase AhpCF (AHP) is specifically required to protect cells incubated under aerobic, phosphate (Pi) starvation conditions. Additional loss of the HP-I (KatG) hydroperoxidase activity dramatically accelerated the death rate of AHP-deficient cells. Investigation of the composition of spent culture media indicates that ,ahpCF katG cells leak nutrients, which suggests that membrane lipids are the principal target of peroxides produced in Pi-starved cells. In fact, the introduction of various mutations inactivating repair activities revealed no obvious role for protein or DNA lesions in the viability of ahp cells. Because the death of ahp cells was directly related to ongoing aerobic glucose metabolism, we wondered how glycolysis, which requires free Pi, could proceed. 31P nuclear magnetic resonance spectra showed that Pi-starved cells consumed Pi but were apparently able to liberate Pi from phosphorylated products, notably through the synthesis of UDP-glucose. Whereas expression of the ahpCF and katG genes is enhanced in an OxyR-dependent manner in response to H2O2 challenge, we found that the inactivation of oxyR and both oxyR and rpoS genes had little effect on the viability of Pi-starved cells. In stark contrast, the inactivation of both oxyR and rpoS genes dramatically decreased the viability of glucose-starved cells. [source] Gilles Deleuze: psychiatry, subjectivity, and the passive synthesis of timeNURSING PHILOSOPHY, Issue 4 2006Marc Roberts rmn diphe ba(hons) pgce pgcrm ma phd student Abstract, Although ,modern' mental health care comprises a variety of theoretical approaches and practices, the supposed identification of ,mental illness' can be understood as being made on the basis of a specific conception of subjectivity that is characteristic of ,modernity'. This is to say that any perceived ,deviation' from this characteristically ,modern self' is seen as a possible ,sign' of ,mental illness', given a ,negative determination', and conceptualized in terms of a ,deficiency' or a ,lack'; accordingly, the ,ideal',therapeutic' aim of ,modern' mental health care can be understood as the ,rectification' of that ,deficiency' through a ,re-instatement' of the ,modern self'. Although contemporary mental health care is increasingly becoming influenced by the so-called ,death' of the ,modern self', this paper will suggest that it is the work of the 20th century French philosopher, Gilles Deleuze, that is able to provide mental health care with a coherent determination of a ,post- modern self'. However, a Deleuzian account of subjectivity stands in stark contrast to ,modernity's' conception of subjectivity and, as such, this paper will attempt to show how this ,post- modern' subjectivity challenges many of the assumptions of ,modern' mental health care. Moreover, acknowledging the complexity and the perceived difficulty of Deleuze's work, this paper will provide an account of subjectivity that can be understood as ,Deleuzian' in its orientation, rather than ,Deleuze's theory of subjectivity', and therefore, this paper also seeks to stimulate further research and discussion of Deleuze's work on subjectivity, and how that work may be able to inform, and possibly even reform, the theoretical foundations and associated diagnostic and therapeutic practices of psychiatry, psychotherapy, and mental health nursing. [source] Framing Anti-Americanism and the Media in South Korea: TV vs Newspaper,PACIFIC FOCUS, Issue 3 2009Yongho Kim This study indicates a stark contrast between the frames by TV news and that of the major newspapers in the coverage of the two candlelight demonstrations in 2002 and 2008 in South Korea. The contrast implies that the frames used by TV news and major newspapers were constructed by two different thematic frames that competed for dominance in public concern: national interest on the one hand, and national prestige and human interests on the other. The TV news framed stories on the 2002 Yangju county accident and the import of American beef in ways to trigger anti-Americanism, which was contrasted with major newspapers' consistent emphasis on national interest. Difference in ownership, target audience and the degree of dependence on advertising sponsors offered plausible explanations for the difference in frames. In addition, the number of journalists going into politics also offered insights into why TV news and newspapers framed the 2002 Yangju county accident and the import of US beef in very unique ways, either by triggering anti-demonstrations or by tranquilizing protests through emphasizing national interests. Overall, difference in frames between major TV news and major newspaper stories represents ideological difference in the public opinion over conservatives versus progressives. [source] Quantum dot-based protein micro- and nanoarrays for detection of prostate cancer biomarkersPROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 9 2008Anisha Gokarna Abstract In this article, we demonstrate the fabrication and detection of cancer protein biochips consisting of micro- and nanoarrays whereby pegylated quantum dots (QDs) conjugated to antibodies (Abs) of prostate specific antigens (PSA) were used for the detection of clinical biomarkers such as PSA. BSA which acts as an efficient blocking layer in microarrays, tends to show an interaction with QDs. In view of this fact, we investigated two series of samples which were fabricated in the presence and absence of BSA blocking layer. Variation in the incubation time required for the antigen,antibody interaction to take place, different proteins as controls and the effect of bare QDs on these microarrays, were the three main parameters which were studied in these two series. Samples fabricated in the absence of BSA blocking layer exhibited an extremely high specificity in the detection of cancer proteins and were also marked by negligible nonspecific binding effects of QDs, in stark contrast to the samples fabricated using BSA as a blocking layer. Fabrication of nanoarrays of QD-conjugated PSA Abs having a spot size of nearly 900,nm has also been demonstrated. Thus, we show the potential offered by QDs in in vitro analysis of cancer biomarker imaging. [source] Salinity stress adaptation competence in the extremophile Thellungiella halophila in comparison with its relative Arabidopsis thalianaTHE PLANT JOURNAL, Issue 5 2005Qingqiu Gong Summary In stark contrast to Arabidopsis, a related species, Thellungiella halophila (Thellungiella salsuginea; salt cress), displays extreme tolerance to high salinity, low humidity and freezing. High nucleotide sequence identity permits the use of tools developed for Arabidopsis for Thellungiella transcript profiling, for which a microarray platform with >25 000 DNA elements (70-mer oligonucleotides) was used. Microarray transcript profiling and intensity analysis, quantitative RT-PCR, and metabolite profiles define genes and pathways that showed shared and divergent responses to salinity stress in the two species. Shared responses are exemplified by 40% of the regulated genes functioning in confining ribosomal functions, photosynthesis and cell growth, as well as activating osmolyte production, transport activities and abscisic acid-dependent pathways. An additional 60% of regulated genes distinguished Thellungiella from Arabidopsis. Analysis of the differences showed that Arabidopsis exhibited a global defense strategy that required bulk protein synthesis, while Thellungiella induced genes functioning in protein folding, post-translational modification and protein redistribution. At 150 mm NaCl, Thellungiella maintained unimpeded growth. Transcript intensity analyses and metabolite profiles supported the microarray results, pointing towards a stress-anticipatory preparedness in Thellungiella. [source] Perception of a cetacean mass stranding in Italy: the emergence of compassionAQUATIC CONSERVATION: MARINE AND FRESHWATER ECOSYSTEMS, Issue 6 2010Giovanni Bearzi Abstract 1The view that whales are malicious monsters has been pervasive throughout history. Conversely, the idea that these animals experience suffering has emerged only recently. One way of investigating perceptual, as well as behavioural, shifts is assessing general public reactions to mortality events involving wild, rare and charismatic animals. 2Here, the responses of 118 individuals to questions regarding the mass stranding of seven sperm whales (Physeter macrocephalus) along the Adriatic Sea coast of Italy in December 2009 are reported through interviews taken at the stranding site and in the direct proximity of the dead animals. 3When asked why the whales were stranded, 44.1% of the respondents suggested anthropogenic causes and 21.2% non-anthropogenic. The remaining 34.7% mentioned a generic ,disorientation' or stated they did not know. When asked how they felt about the whales, 68.6% expressed feelings of compassion or care towards the animals. Clearly non-compassionate attitudes accounted for only 4.1% of the sample. Finally, 21.2% expressed feelings that were ambiguous in terms of being suggestive of compassionate or non-compassionate attitudes, including 11.9% amazement, 4.2% deprecation and 5.1% powerlessness. 4These results are in stark contrast with information obtained from accounts of similar events that have occurred in historical times, up until the first half of the 20th century. For centuries, responses to cetacean live strandings,typically including killing and harming of the animals,were either utilitarian or characterized by feelings including fear and a desire to ,subjugate the beast', with no apparent concern for their suffering and death. 5It is concluded that attitudes towards whales,today strikingly revolving around sadness, compassion and a sense of loss,have changed dramatically over time, with a steep turnaround in the 1970/1980s. Full appreciation of the ongoing evolution in public perception can channel marine conservation efforts and assist in the design of response strategies to marine mammal strandings. Copyright © 2010 John Wiley & Sons, Ltd. [source] Invariability of central metabolic flux distribution in Shewanella oneidensis MR-1 under environmental or genetic perturbationsBIOTECHNOLOGY PROGRESS, Issue 5 2009Yinjie J. Tang Abstract An environmentally important bacterium with versatile respiration, Shewanella oneidensis MR-1, displayed significantly different growth rates under three culture conditions: minimal medium (doubling time ,3 h), salt stressed minimal medium (doubling time ,6 h), and minimal medium with amino acid supplementation (doubling time ,1.5 h). 13C-based metabolic flux analysis indicated that fluxes of central metabolic reactions remained relatively constant under the three growth conditions, which is in stark contrast to the reported significant changes in the transcript and metabolite profiles under various growth conditions. Furthermore, 10 transposon mutants of S. oneidensis MR-1 were randomly chosen from a transposon library and their flux distributions through central metabolic pathways were revealed to be identical, even though such mutational processes altered the secondary metabolism, for example, glycine and C1 (5,10-Me-THF) metabolism. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009 [source] Academic Paths, Ageing and the Living Conditions of Students in the Late 20th Century,CANADIAN REVIEW OF SOCIOLOGY/REVUE CANADIENNE DE SOCIOLOGIE, Issue 2 2001Arnaud Sales Le monde étudiant est profondément marqué par la diversité des tra-jectoires individuelles, qui sont souvent très éloignées d'un déroule-ment linéaire des études. Les conséquences sur l'âge des étudiants sont majeures, et la condition étudiante ne peut plus être définie comme une expérience strictement juvénile. Or, entre 20 et 30 ans, l'âge engendre des impératifs différentiels sur le plan des conditions et des modes de vie qui ne sont pas toujours compatibles avec la condition étudiante classique. Cette étude des parcours et de la situation financière des étudiants des universités québécoises de langue françhise et anglaise montre comment s'opère la déconnexion entre jeunesse et condition étudiante, et comment cette déconnexion influe sur la différenciation des conditions de vie et de financement des études. Student life is profoundly marked by the diversity of individual trajectories, which are in stark contrast with the linear path traditionally taken by students. The impact on the age of the student population is significant: indeed, student life can no longer be qualified as strictly for the young. Between the ages of 20 and 30 years, different imperatives come into play in terms of living conditions and lifestyle. These imperatives are not always compatible with the conditions of classic student life. This study of the academic paths and the financial situation of Quebec university students shows how the disconnection between student condition and youth occurs and how this disconnection impacts the differentiation of student's living conditions and modes of financing university studies. [source] Thermodynamic/Kinetic Control in the Isomerization of the [{tBuNP(,-NtBu)}2]2, IonCHEMISTRY - A EUROPEAN JOURNAL, Issue 9 2004Andrew D. Bond Dr. Abstract The unique structure of [(tBuN)2PK], (2) (containing [(tBuN)2P], monoanions) is in stark contrast to the previously reported Li+ analogue [{[tBuNP(,-NtBu)]2}2]Li4 (1) (containing the dimeric [{tBuNP(,-NtBu)}2]2, ion). DFT and 31P NMR spectroscopic studies reveal that the formation of the monoanion arrangements are most thermodyamically favored for Li, Na, and K, 1 being the product of kinetic control and 2 being the product of thermodynamic control. [source] Cottage industry, migration, and marriage in nineteenth-century EnglandECONOMIC HISTORY REVIEW, Issue 4 2008NIGEL GOOSE There has been considerable debate concerning the impact of the industrial employment of women upon their demographic behaviour in nineteenth-century England. This article assesses the impact of employment in the cottage industry of straw plait and hat making in the county of Hertfordshire, comparing and contrasting districts where the industry was prominent with those where it was not. It is discovered that in 1851 the availability of straw industry employment encouraged earlier marriage, most notably in those parishes where the industry was particularly heavily concentrated, although overall levels of nuptiality and proportions ultimately marrying were similar in straw and non-straw areas alike. By 1871, however, the skewed sex ratio that such employment produced among young adults served to offset this positive effect. As the industry waned in the later nineteenth century, the experience of different regions of the county converged, while throughout the period the data suggest that urban/rural contrasts and the suburbanization of London produced more stark contrasts in female marriage patterns than did the availability of cottage industry employment. [source] Comprehensiveness versus Pragmatism: Consensus at the Japanese,Dutch InterfaceJOURNAL OF MANAGEMENT STUDIES, Issue 8 2007Niels G. Noorderhaven abstract By comparing the views of managers working at the interface of two consensus-oriented societies, Japan and the Netherlands, we show important differences between the consensus decision-making processes as seen by Japanese and Dutch managers. These differences relate to how complete the agreement of opinion should be in order to speak of consensus, with the Japanese managers demanding a more complete consensus than the Dutch. The processes and conditions that Japanese and Dutch managers see as leading to consensus also differ. Japanese consensus is based on a more ordered, sequential process than Dutch consensus. Our respondents differed deeply regarding the role of the hierarchy in their own and the others' consensus processes, with both Japanese and Dutch managers seeing their own consensus process as less hierarchical. Our findings show that the concept of consensus is interpreted quite differently by Japanese and Dutch managers. This is an important warning for companies operating at the interface of these two societies. More in general our research illustrates the usefulness for international management research of detailed comparative studies focusing not on stark contrasts but on more subtle differences between management practices. [source] | ||||||||||||||||||||||