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Stage Larvae (stage + larva)
Selected AbstractsAntigen selection for future anti- Trichuris vaccines: a comparison of cytokine and antibody responses to larval and adult antigen in a primary infectionPARASITE IMMUNOLOGY, Issue 9 2008H. DIXON SUMMARY Trichuriasis, caused by the whipworm Trichuris trichiura, is endemic in tropical and subtropical areas, affecting approximately 1 billion people. Child anthelminthic treatment programmes are being implemented but repeated treatments are costly, may prevent the development of acquired immunity and can lead to the development of drug resistant parasites. Thus, the development of a vaccine which would lead to the acquisition of immunity at an earlier age and reduce community faecal egg output would be beneficial. Development of subunit vaccines requires the identification of protective antigens and their formulation in a suitable adjuvant. Trichuris muris is an antigenically similar laboratory model for T. trichiura. Subcutaneous vaccination with adult excretory,secretory products (ES) protects susceptible mouse strains from T. muris. Larval stages may contain novel and more relevant antigens which when incorporated in a vaccine induce worm expulsion earlier in infection than the adult worm products. This study finds negligible difference in the cellular and humoral immune response to T. muris adult and third stage larva(e) (L3) ES during a primary T. muris infection, but identifies high molecular weight proteins in both adult and L3 ES as potential vaccine candidates. [source] Effects of Dietary Heat-killed Lactobacillus plantarum on Larval and Post-larval Kuruma Shrimp, Marsupenaeus japonicus BateJOURNAL OF THE WORLD AQUACULTURE SOCIETY, Issue 2010Ha Thanh Tung Two feeding trials were conducted to evaluate the effects of heat-killed Lactobacillus plantarum (HK-LP) on larval and post-larval kuruma shrimp, Marsupenaeus japonicus Bate. Five microbound diets were formulated to contain levels of a preparation containing 20% HK-LP (HK-LP Prep): 0, 0.001, 0.01, 0.1, and 1 g/kg. In the first experiment, zoea1 stage larvae were fed test diets for 8 d. Some parameters such as survival, developmental stage, metamorphosis to post-larvae, and formalin stress resistance were evaluated. In second trial, post-larval shrimp (16 ± 0.04 mg) were fed test diets for 30 d. Survival, body weight gain, specific growth rate, feed efficiency ratio, individual dry weight, total body length, osmotic and formalin stress resistances, and protease activity were evaluated. Results indicated that the larvae that received HK-LP Prep at 0.1 and 1 g/kg diets showed significantly (P < 0.05) higher survival than that of the control group. For the post-larval trial, shrimp that received HK-LP Prep at 1 g/kg diet were significantly higher than the control group in most of the growth parameters and stress resistance. However, shrimp that received HK-LP Prep at 0.1 g/kg diet were significantly higher than the control group in survival only. For protease activity, there was no significant difference detected among groups. [source] Protective immunization of calves against Ostertagia ostertagi using fourth stage larval extractsPARASITE IMMUNOLOGY, Issue 9-10 2010A. M. HALLIDAY Summary ConA lectin was used to isolate glycoproteins from detergent extracts of fourth stage Ostertagia ostertagi larvae. This preparation contained proteins additional to those observed in a similar fraction prepared from adult O. ostertagi. Two vaccine trials were conducted with this preparation, and sub-fractions thereof, in groups of 6,8 worm-free calves. All groups were challenged with 50 000 O. ostertagi larvae 1 week after the final immunization, and protection was assessed by comparing the egg and worm counts of the immunized groups with their respective controls. Immunization with the ConA-binding antigen or its sub-fractions induced high titre serum antibody responses. In the first trial, the cumulative egg count of the group immunized with unfractionated antigen was 60% lower than the corresponding control value, and worm counts were 47% lower. In the second trial, the cumulative egg counts of the vaccinated groups ranged from 70% to 85% lower than the corresponding controls, with worm counts up to 64% lower. It was concluded that detergent-soluble, ConA-binding extracts prepared from O. ostertagi fourth stage larvae contained protective immunogens that were as effective as the best antigens published for O. ostertagi to date. [source] Angiostrongylus vasorum (French heartworm) in a dog imported into AustraliaAUSTRALIAN VETERINARY JOURNAL, Issue 1-2 2007AI Tebb This report describes a case of Angiostonglyus vasorum infection, sometimes referred to as French heartworm disease, in a dog imported into Western Australia from the United Kingdom. Diagnosis was made by identification of first stage larvae on fine needle aspiration of a consolidated lung lobe. First stage larvae were also identified in the faeces by the Baermann technique. The main clinical signs in this case were coughing and tachypnoea. The dog was treated successfully with fenbendazole at 50 mg/kg once daily for 10 days. [source] |