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Spot Resistance (spot + resistance)
Selected AbstractsMagnaporthe oryzae isolates causing gray leaf spot of perennial ryegrass possess a functional copy of the AVR1-CO39 avirulence geneMOLECULAR PLANT PATHOLOGY, Issue 3 2006REBECCA PEYYALA SUMMARY Gray leaf spot of perennial ryegrass (Lolium perenne) is a severe foliar disease caused by the ascomycete fungus Magnaporthe oryzae (formerly known as Magnaporthe grisea). Control of gray leaf spot is completely dependent on the use of fungicides because currently available perennial ryegrass cultivars lack genetic resistance to this disease. M. oryzae isolates from perennial ryegrass (prg) were unable to cause disease on rice cultivars CO39 and 51583, and instead triggered a hypersensitive response. Southern hybridization analysis of DNA from over 50 gray leaf spot isolates revealed that all of them contain sequences corresponding to AVR1-CO39, a host specificity gene that confers avirulence to rice cultivar CO39, which carries the corresponding resistance gene Pi-CO39(t). There was also an almost complete lack of restriction site polymorphism at the avirulence locus. Cloning and sequencing of the AVR1-CO39 gene (AVR1-CO39Lp) from 16 different gray leaf spot isolates revealed just two point mutations, both of which were located upstream of the predicted open reading frame. When an AVR1-CO39Lp gene copy was transferred into ML33, a rice pathogenic isolate that is highly virulent to rice cultivar CO39, the transformants were unable to cause disease on CO39 but retained their virulence to 51583, a rice cultivar that lacks Pi-CO39(t). These data demonstrate that the AVR1-CO39 gene in the gray leaf spot pathogens is functional, and suggest that interaction of AVR1-CO39Lp and Pi-CO39(t) is responsible, at least in part, for the host specificity expressed on CO39. This indicates that it may be possible to use the Pi-CO39(t) resistance gene as part of a transgenic strategy to complement the current deficiency of gray leaf spot resistance in prg. Furthermore, our data indicate that, if Pi-CO39(t) can function in prg, the resistance provided should be broadly effective against a large proportion of the gray leaf spot pathogen population. [source] Molecular diversity and association of SSR markers to rust and late leaf spot resistance in cultivated groundnut (Arachis hypogaea L.)PLANT BREEDING, Issue 1 2010S. Mondal With 1 figure and 2 tables Abstract Molecular diversity and association of simple sequence repeat (SSR) markers with rust and late leaf spot (LLS) resistance were detected in a set of 20 cultivated groundnut genotypes differing in resistance against both diseases. Out of 136 bands amplified from 26 primers, 104 were found polymorphic (76.5%). Cluster analysis (UPGMA) revealed two main clusters separated at 52% Jaccard's similarity coefficient according to disease reaction to rust and LLS. Based on the Kruskal,Wallis one-way anova and simple regression analysis three and four SSR alleles were found associated with rust and LLS resistance, respectively. [source] Evaluation of common wheat cultivars for tan spot resistance and chromosomal location of a resistance gene in the cultivar ,Salamouni'PLANT BREEDING, Issue 4 2006W. Tadesse Abstract A total of 50 wheat (Triticum aestivum L.) cultivars were evaluated for resistance to tan spot, using Pyrenophora tritici-repentis race 1 and race 5 isolates. The cultivars ,Salamouni', ,Red Chief', ,Dashen', ,Empire' and ,Armada' were resistant to isolate ASC1a (race 1), whereas 76% of the cultivars were susceptible. Chi-squared analysis of the F2 segregation data of hybrids between 20 monosomic lines of the wheat cultivar ,Chinese Spring' and the resistant cultivar ,Salamouni' revealed that tan spot resistance in ,Salamouni' was controlled by a single recessive gene located on chromosome 3A. This gene is designated tsn4. The resistant cultivars identified in this study are recommended for use in breeding programmes to improve tan spot resistance in common wheat. [source] Monosomic addition lines of Beta corolliflora in sugar beet: plant morphology and leaf spot resistancePLANT BREEDING, Issue 1 2002D. Gao Abstract Monosomic addition lines in Beta vulgaris from Beta corolliflora were described morphologically and characterized for disease resistance. Monosomic addition plants (2n= 19) were selected among segregating offspring by a squash dot technique in combination with B. corolliflora -specific probes. Plants carrying an added chromosome were characterized by leaf shape, plant size and plant vigour. In this way, most addition lines could be distinguished from diploid beets, however, to identify those plants unequivocally, molecular marker analysis was also necessary. Transmission frequencies of each addition line were determined to be in the range 13.9% (Cor-4) to 60% (Cor-9). High transmission rate of addition line Cor-9 was assumed to be due to apomictic propagation because transmission rate after selfing cannot exceed 50%. Cercospora leaf spot resistance tests were performed on 167 monosomic plants from seven different addition lines, two fragment addition lines and 89 diploid controls. No line exhibited complete resistance, but the monosomic additions Cor-3 and Cor-4 showed significantly lower infection rates than their diploid sibling plants. The identification of monosomic addition lines with apomictic and disease resistance characters offers the possibility of transferring those genes to sugar beet. [source] | ||||||||||