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Spermatozoa Motility (spermatozoa + motility)

Distribution by Scientific Domains
Earth and Environmental Science100%

Selected Abstracts

Protein profile study in European eel (Anguilla anguilla) seminal plasma and its correlation with sperm quality

JOURNAL OF APPLIED ICHTHYOLOGY, Issue 5 2010
D. S. Peņaranda
Summary Along with sperm quality parameters, the protein profile of European eel seminal plasma was analyzed during induced spermiation (n = 56 samples). Motility, Percentage of live cells, spermatozoa head morphometry and concentration showed low values during the initial weeks of spermiation and maintained high levels throughout the rest of the experiment. The protein profile gradient by SDS-PAGE (4,15%) registered four important electrophoretic bands around 80, 40, 26 and 12 KDa. Three of them showed significant differences in concentration during treatment (80, 40 and 12 KDa), and all of them showed the highest value on the 8th week. Both 80 and 12 KDa bands increased until the 8th week, followed by a progressive decline. One possible explanation for these profiles is that, in the first weeks of treatment, proteins originated from blood plasma are accumulated in the seminal plasma, and from the 8th week some of these proteins are incorporated into the spermatic membranes. The 40 KDa protein band also increased during the first 8 weeks, but maintained high concentrations in the seminal plasma for the rest of the experiment. One result confirms the theory that the presence of proteins in the seminal plasma having a molecular weight lower than 50 KDa increased spermatozoa motility, since the 40 KDa band displayed significantly higher values coinciding with the high percentages of spermatozoa motility. Seminal plasma proteins seem to have an important role in spermatogenesis and spermatozoa movement, but further studies are necessary to discover the identity of these proteins and their precise functions. [source]

Dietary n-3/n-6 ratio affects the biochemical composition of Eurasian perch (Perca fluviatilis) semen but not indicators of sperm quality

AQUACULTURE RESEARCH, Issue 9 2010
Emilie Henrotte
Abstract In general, the effects of dietary fatty acids (FA) on sperm quality have received less attention than egg quality, and were never studied in perch. This study investigated the effects of dietary FAs on the quality and chemical composition of sperm in Eurasian perch (Perca fluviatilis). Two experimental diets containing 16% lipids and 45% proteins were compared. The n-3/n-6 ratios tested were 0.2 for diet 1 (D1) and 7.0 for diet 2 (D2). No significant effects of the n-3/n-6 ratio were observed on the sperm characteristics, either in terms of the sperm volume (around 1.2 mL) and density, spermatozoa motility (94%) and velocity, or the sperm osmolality. All these parameters corresponded to semen of good quality in Eurasian perch. Interestingly, both the FA composition and the lipid class profile of the semen were correlated to the tested diet. However, basal levels of certain highly unsaturated FAs such as eicosapentaenoic acid, 20:5 n-3 and docosahexaenoic acid, 22:6 n-3, were maintained in the sperm irrespective of the diet tested. Perch semen was characterized by high levels of cholesterol, phosphatidylethanolamine and phosphatidylcholine. In conclusion, the dietary n-3/n-6 ratio affects the lipid composition of perch semen but not the indicators of sperm quality. [source]

Sperm motility and fertilizing ability in the Persian sturgeon Acipenser persicus

AQUACULTURE RESEARCH, Issue 9 2005
Sayyed Mohammad Hadi Alavi
Abstract The motility and fertilizing ability of the Persian sturgeon, Acipenser persicus, spermatozoa were investigated. Optimum ionic content (Na+, K+, Ca2+ and Mg2+) and pH of activation solution as well as the optimum dilution rate were determined. The results show optimum motility characteristics of spermatozoa in buffered solutions containing 25, 0.2, 3 and 10 mM L,1 Na+, K+, Ca2+ and Mg2+, respectively, at dilution rate 1:50 and pH 8.0. To test the fertilizing ability of sperm, two buffered saline solutions were used as activation solution of sperm motility. The present study indicated (1) spermatozoa motility is one of key factors that influence on fertilizing ability of sperm, (2) a high fertilizing ability of sperm is obtained after dilution in saline solutions rather than in freshwater and (3) a maximum fertilization rate occurs in buffered saline solution containing 0.2 mM L,1 K+. There is also a good correlation between biochemical characteristics of seminal plasma and fertilizing ability of sperm. [source]

Some characteristics and short-term preservation of spermatozoa of Deccan mahseer, Tor khudree (Sykes)

AQUACULTURE RESEARCH, Issue 5 2005
Nagappa Basavaraja
Abstract Over the years, the natural stocks of Tor khudree (Cyprinidae) have depleted due to anthropogenic activities and hence it is considered a threatened species in India. Several in situ and ex situ conservation strategies have been suggested for the revival of T. khudree stocks. The total volume of milt obtained from hormone-injected (gonadotropin-releasing hormone analogue+domperidone) T. khudree were significantly higher (six to seven times) than that of uninjected fish. The total number of spermatozoa per fish was also higher in injected fish (6.5 × 108,7.6 × 108) than that obtained from uninjected counterparts (1.3 × 108,1.8 × 108). On the contrary, the spermatozoa density and spermatocrit were found to be lower in injected fish than that of the controls. Spermatozoa density and spermatocrit ranged between 4.1 × 108,4.4 × 108 spermatozoa mL,1 and 38.1,39.4%, respectively, in injected fish, whereas the figures fluctuated between 6.0 × 108,7.8 × 108 spermatozoa mL,1 and 61.5,63.1%, respectively, in uninjected fish. However, there was no significant difference in the spermatozoa motility rates between experimental and control fish. Different spermatozoa-activating media revealed no significant difference in spermatozoa motility between hormone-injected and uninjected mahseers. But motility duration was the longest with NaCl+urea (190,193 s) and the shortest with tap water (50,55 s) in the experimental and control groups. Short-term preservation of the spermatozoa of T. khudree indicated that spermatozoa stored at 4°C had higher motility rates than those preserved at room temperature either in the presence or absence of oxygen. [source]