THE QUARTERLY JOURNAL OF THE ROYAL METEOROLOGICAL SOCIETY, Issue 646 2010
J. Thuburn
Abstract In a recent paper, a conservative semi-Lagrangian mass transport scheme SLICE has been coupled to a semi-implicit semi-Lagrangian scheme for the shallow-water equations. The algorithm involves the solution at each timestep of a nonlinear Helmholtz problem, which is achieved by iterative solution of a linear ,inner' Helmholtz problem; this framework, as well as the linear Helmholtz operator itself, are the same as would be used with a non-conservative interpolating semi-Lagrangian scheme for mass transport. However, in order to do this, a reference value of geopotential was introduced into the discretization. It is shown here that this results in a weak dependence of the results on that reference value. An alternative coupling is therefore proposed that preserves the same solution framework and linear Helmholtz operator but, at convergence of the nonlinear solver, has no dependence on the reference value. However, in order to maintain accuracy at large timesteps, this approach requires a modification to how SLICE performs its remapping. An advantage of removing the dependence on the reference value is that the scheme then gives consistent tracer transport. Copyright © 2010 Royal Meteorological Society and Crown Copyright. Published by John Wiley & Sons, Ltd. [source]

An inherently mass-conserving semi-implicit semi-Lagrangian discretisation of the shallow-water equations on the sphere

THE QUARTERLY JOURNAL OF THE ROYAL METEOROLOGICAL SOCIETY, Issue 642 2009
M. Zerroukat
Abstract For the shallow-water equations on the sphere, an inherently mass-conserving semi-Lagrangian discretisation (SLICE) of the continuity equation is coupled with a semi-implicit semi-Lagrangian discretisation of the momentum equations. Various tests from the literature (two with analytical nonlinear solutions) are used to assess the model's performance and also to compare it with that of a variant model that instead employs a standard non-conserving semi-implicit semi-Lagrangian discretisation of the continuity equation. The mass-conserving version gives results that are overall somewhat better than the non-conserving one. Copyright © 2009 Royal Meteorological Society and Crown Copyright [source]

A monotonic and positive,definite filter for a Semi-Lagrangian Inherently Conserving and Efficient (SLICE) scheme

THE QUARTERLY JOURNAL OF THE ROYAL METEOROLOGICAL SOCIETY, Issue 611 2005
Mohamed Zerroukat
Abstract A new monotonic and positive,definite filter is incorporated into an existing Semi-Lagrangian Inherently Conserving and Efficient (SLICE) scheme for transport problems in both Cartesian and spherical geometry. The SLICE scheme is based on a control-volume approach that uses multiple sweeps of a one-dimensional O (,x4) conservation remapping algorithm along predetermined cascade directions. The new filter combines a selective detection algorithm, to pinpoint regions of non-monotonic behaviour, with a hierarchical reduction of the degree of the piecewise reconstruction in such regions, to re-establish monotonicity. The enhanced, monotonic and positive,definite, SLICE scheme is tested in one dimension, and then applied to standard two-dimensional test problems in both Cartesian and spherical geometries. Comparisons with published results of other conservative semi-Lagrangian schemes show that it performs well. © Crown copyright, 2005. [source]

SLICE-S: A Semi-Lagrangian Inherently Conserving and Efficient scheme for transport problems on the Sphere

THE QUARTERLY JOURNAL OF THE ROYAL METEOROLOGICAL SOCIETY, Issue 602 2004
Mohamed Zerroukat
Abstract The Semi-Lagrangian Inherently Conserving and Efficient (SLICE) scheme developed for Cartesian geometry is generalized to spherical geometry. The spherical version, SLICE-S, is similarly based on a Control Volume approach and multiple sweeps of a one-dimensional O(,s4) (where s is the spherical distance) conservative remapping algorithm along Eulerian latitudes, then along Lagrangian longitudes. The resulting conservative scheme requires no restriction on either the polar meridional or zonal Courant numbers. SLICE-S is applied to the standard problems of solid-body rotation and deformational flow, and results are compared with those of a standard non-conservative and other published conservative semi-Lagrangian schemes. In addition to mass conservation, and consistent with the performance of SLICE, the present scheme is competitive in terms of accuracy and efficiency. © Crown copyright, 2004. Royal Meteorological Society [source]

OPTIMIZATION OF PRE-FRY DRYING OF YAM SLICES USING RESPONSE SURFACE METHODOLOGY

JOURNAL OF FOOD PROCESS ENGINEERING, Issue 4 2010
OLAJIDE PHILIP SOBUKOLA
ABSTRACT The effect of convective hot-air drying pretreatment and frying time at a frying temperature of 170 ± 1C on moisture and oil contents, breaking force (crispness) and color parameters of yam chips was investigated. Response surface methodology technique was used to develop models for the responses as a result of variation in levels of drying temperature (60,80C), drying time (1,5°min) and frying time (2,6°min). Drying pretreatment had a significant effect on oil and moisture contents, breaking force and color parameters of yam chips, with water removal exhibiting a typical drying profile. Response surface regression analysis shows that responses were significantly (P < 0.05) correlated with drying temperature and time and frying time. The optimum pre-fry drying condition observed was a drying temperature of 70,75C for about 3,4 min while frying for 4,5 min. PRACTICAL APPLICATIONS Deep-fat frying is a very important cooking method and a lot of effort has been devoted to manufacturing fried products with lower oil content and acceptable quality parameters. The information provided in this work will be very useful in manufacturing fried yam chips of acceptable quality attributes through the combination of drying pretreatment conditions. The result is very useful in considering different processing variables and responses at the same time as compared with single factor experiment common in the literature. [source]

MATHEMATICAL MODELLING OF THIN-LAYER DRYING OF KIWIFRUIT SLICES

JOURNAL OF FOOD PROCESSING AND PRESERVATION, Issue 2009
M DOYMAZ
ABSTRACT The effect of temperature on the drying kinetics of kiwifruit was investigated. The drying process was carried out at temperatures of 50, 55 and 60C, air velocity of 2.4 m/s and relative humidity between 10,25%. Drying time decreased considerably with increased air temperature. Drying process took place in the falling rate period. Twelve mathematical models available in the literature were fitted to the experimental data. The models were compared by four statistical parameters; i.e., determination of coefficient, mean relative percent error, reduced chi-square and root mean square error, and the best-fit model was selected. The modified Henderson and Pabis and Verma et al. models were given the best results in describing thin-layer drying of kiwifruits. The effective diffusivity of water during air-drying varied from 1.743 to 2.241 × 10,10 m2/s over the temperature range investigated, with activation energy equal to 22.48 kJ/mol. PRACTICAL APPLICATIONS Drying can be described as an industrial preservation method in which water content and activity of agricultural products are decreased by heated air to minimize biochemical, chemical and microbiological deterioration. Kiwifruit has a very short life because of softening and vitamin loss during cold storage. The use of drying prolongs the shelf-life of the kiwifruit, as the water content reduction slows down deterioration reactions. In this study, drying characteristics of kiwifruits were studied in a convectional hot-air dryer. The objectives of the present study were to determine experimentally the thin-layer drying characteristics and rehydration capacity of samples, and to fit the experimental data to 12 mathematical models available from the literature. [source]

EFFECT OF STORAGE TEMPERATURE ON THE GROWTH OF LISTERIA MONOCYTOGENES ON QUESO BLANCO SLICES,

JOURNAL OF FOOD SAFETY, Issue 3 2006
GAYLEN A. UHLICH
ABSTRACT A five-strain cocktail of Listeria monocytogenes (104 cfu/mL) was inoculated onto individual vacuum-packaged slices (ca. 50 g each) of a commercial, Hispanic-style cheese, that being Queso Blanco. Growth was determined at appropriate intervals during storage at 5, 10, 15, 20 and 25C. In general, as the incubation temperature increased, a shorter lag phase duration (LPD) and a faster growth rate (GR) were observed. The LPD values at 5, 10, 15, 20 and 25C were 65.3, 19.9, 2.1, 8.4 and 11.4 h, respectively. The GR values were 0.011, 0.036, 0.061, 0.090 and 0.099 log cfu/h at 5, 10, 15, 20 and 25C, respectively. There were no statistical differences in LPD at 10, 15, 20 and 25C. However, the LPD during growth at 5C was statistically (P , 0.05) longer than at all other temperatures. The GR values at 20 and 25C were not significantly different from each other, whereas the GR values at 5, 10 and 15C were significantly different from each other as well as from the GR at 20 and 25C (P , 0.05). The maximum population density (MPD) showed relatively little variation over the range of storage temperatures tested, with an average of 8.38 log cfu/g (SD = 0.33). The results of this study indicate that not even the lowest trial temperature of 5C prevented growth over time of the inoculated L. monocytogenes on this sliced product, and that proper storage and handling procedures are required to prevent the bacterium from contaminating the product and/or to control its growth. [source]

Changes in epidemiological patterns of sea lice infestation on farmed Atlantic salmon, Salmo salar L., in Scotland between 1996 and 2006

JOURNAL OF FISH DISEASES, Issue 4 2008
F Lees
Abstract Analyses of a unique database containing sea lice records over an 11 year period provide evidence of changing infestation patterns in Scotland. The data, collected from more than 50 commercial Atlantic salmon farms, indicate that both species of sea lice commonly found in Scotland, Lepeophtheirus salmonis and Caligus elongatus, have declined on farms over the past decade. Reductions for both species have been particularly marked since 2001 when more effective veterinary medicines became available. Treatment data were also available in the database and these show a growing trend towards the use of the in-feed medication emamectin benzoate (Slice®), particularly in the first year of the salmon production cycle. However, this trend towards single product use has not been sustained in 2006, the latest year for which data are available. There is some evidence of region to region variation within Scotland with the Western Isles experiencing higher levels of infestation. However, compared to the levels observed between 1996 and 2000, all regions have benefited from reduced lice infestation, with the overall pattern showing a particular reduction in the second and third quarters of the second year of production. [source]

Bread, a Slice of History

QUALITY ASSURANCE & SAFETY OF CROPS & FOOD, Issue 1 2010
Linda S. Young
No abstract is available for this article. [source]

Cooling Abolishes Neuronal Network Synchronization in Rat Hippocampal Slices

EPILEPSIA, Issue 6 2002
Sam P. Javedan
Summary: ,Purpose: We sought to determine whether cooling brain tissue from 34 to 21°C could abolish tetany-induced neuronal network synchronization (gamma oscillations) without blocking normal synaptic transmission. Methods: Intracellular and extracellular electrodes recorded activity in transverse hippocampal slices (450,500 ,m) from Sprague,Dawley male rats, maintained in an air,fluid interface chamber. Gamma oscillations were evoked by afferent stimulation at 100 Hz for 200 ms. Baseline temperature in the recording chamber was 34°C, reduced to 21°C within 20 min. Results: Suprathreshold tetanic stimuli evoked membrane potential oscillations in the 40-Hz frequency range (n = 21). Gamma oscillations induced by tetanic stimulation were blocked by bicuculline, a ,-aminobutyric acid (GABA)A -receptor antagonist. Cooling from 34 to 21°C reversibly abolished gamma oscillations in all slices tested. Short, low-frequency discharges persisted after cooling in six of 14 slices. Single-pulse,evoked potentials, however, were preserved after cooling in all cases. Latency between stimulus and onset of gamma oscillation was increased with cooling. Frequency of oscillation was correlated with chamber cooling temperature (r = 0.77). Tetanic stimulation at high intensity elicited not only gamma oscillation, but also epileptiform bursts. Cooling dramatically attenuated gamma oscillation and abolished epileptiform bursts in a reversible manner. Conclusions: Tetany-induced neuronal network synchronization by GABAA -sensitive gamma oscillations is abolished reversibly by cooling to temperatures that do not block excitatory synaptic transmission. Cooling also suppresses transition from gamma oscillation to ictal bursting at higher stimulus intensities. These findings suggest that cooling may disrupt network synchrony necessary for epileptiform activity. [source]

Low Concentration of DL-2-Amino-5-phosphonovalerate Induces Epileptiform Activity in Guinea Pig Hippocampal Slices

EPILEPSIA, Issue 10 2001
Ali Gorji
Summary: , Purpose: The specific mechanisms by which low concentrations of cyclosporine induce seizures and low concentrations of phencyclidine provoke behavioral excitation remain to be elucidated. Both compounds block N -methyl- d -aspartate (NMDA) receptors. The aim of this study was to determine if low concentrations of the NMDA-receptor blockers increase the seizure susceptibility. Methods: Guinea pig hippocampal slices were exposed to artificial cerebrospinal fluid containing the NMDA blocker dl -2-amino-5-phosphono-valerate (APV; 0.1,10 ,M). Extracellular field potentials were recorded from CA1 and CA3 regions. Results: Low concentrations of APV induced epileptiform burst discharges (0.1,0.25 ,M), whereas higher doses failed to decrease the seizure threshold (1,10 ,M). Conclusions: The results indicate that the excitatory effect of low concentrations NMDA blockers may play a role in the neurotoxicity of aforementioned substances. [source]

Valproate Prevents Epileptiform Activity After Trauma in an In Vitro Model in Neocortical Slices

EPILEPSIA, Issue 12 2000
Article first published online: 30 JAN 200
First page of article [source]

Rapid Cooling Aborts Seizure-Like Activity in Rodent Hippocampal-Entorhinal Slices

EPILEPSIA, Issue 10 2000
Matthew W. Hill
Summary Purpose: As a preliminary step in the development of an implantable Peltier device to abort focal neocortical seizures in vivo, we have examined the effect of rapid cooling on seizures in rodent hippocampal-entorhinal slices. Methods: Seizure-like discharges were induced by exposing the slices to extracellular saline containing 4-aminopyridine (50 ,mol/L). Results: When we manually activated a Peltier device that was in direct contact with the slice, seizures terminated within seconds of the onset of cooling, sometimes preceding a detectable decrease in temperature measured near the top of the slice. However, activation of the Peltier device did not stop seizures when slices were no longer in direct physical contact with the device, indicating that this was not a field effect. When cooling was shut off and temperature returned to 33oC, bursting some-times returned, but a longer-term suppressive effect on seizure activity could be observed. In two of our experiments, a custom computer program automatically detected seizure discharges and triggered a transistor-transistor logic pulse to activate the Peltier device. In these experiments, the Peltier device automatically terminated the slice bursting in less than 4 seconds. When the Peltier device was placed in contact with the normal, exposed cortex of a newborn pig, we found that the cortical temperature decreased rapidly from 36oC to as low as 26oC at a depth of 1.7 mm below the cooling unit. Conclusions: These experiments show that local cooling may rapidly terminate focal paroxysmal discharges and might be adapted for clinical practice. [source]

Quantitative effects produced by modifications of neuronal activity on the size of GABAA receptor clusters in hippocampal slice cultures

EUROPEAN JOURNAL OF NEUROSCIENCE, Issue 2 2004
Serge Marty
Abstract The number and strength of GABAergic synapses needs to be precisely adjusted for adequate control of excitatory activity. We investigated to what extent the size of GABAA receptor clusters at inhibitory synapses is under the regulation of neuronal activity. Slices from P7 rat hippocampus were cultured for 13 days in the presence of bicuculline or 4-aminopyridine (4-AP) to increase neuronal activity, or DNQX to decrease activity. The changes provoked by these treatments on clusters immunoreactive for the ,1 and ,2 subunits of the GABAA receptor or gephyrin were quantitatively evaluated. While an increase in activity augmented the density of these clusters, a decrease in activity provoked, in contrast, a decrease in their density. An inverse regulation was observed for the size of individual clusters. Bicuculline and 4-AP decreased whilst DNQX increased the mean size of the clusters. When the pharmacological treatments were applied for 2 days instead of 2 weeks, no effects on the size of the clusters were observed. The variations in the mean size of individual clusters were mainly due to changes in the number of small clusters. Finally, a regulation of the size of GABAA receptor clusters occurred during development in vivo, with a decrease of the mean size of the clusters between P7 and P21. This physiological change was also the result of an increase in the number of small clusters. These results indicate that neuronal activity regulates the mean size of GABAA receptor- and gephyrin-immunoreactive clusters by modifying specifically the number of synapses with small clusters of receptors. [source]

Effects of additional and extended acid etching on bonding to caries-affected dentine

EUROPEAN JOURNAL OF ORAL SCIENCES, Issue 5 2004
César Augusto Galvão Arrais
This study evaluated the effects of additional and extended acid etching on microtensile bond strength (µTBS) of two adhesive systems to sound (SD) and caries-affected dentine (CAD). Flat surfaces of CAD surrounded by SD of 36 extracted carious third molars were assigned to four treatments (i): self-etching adhesive system (Clearfil SE Bond) applied to dentine surfaces following manufacturer's instructions (MI); (ii) after additional etching for 15 s (35% phosphoric acid, PA); (iii) total-etch one-bottle adhesive (Single Bond) applied to dentine surfaces following MI; or (iv), after etching for 45 s with PA. Composite ,blocks' were built on bonded surfaces and restored teeth were vertically sectioned to obtain bonded slices of 0.7 mm thick. Slices were trimmed to create hourglass-shaped specimens (cross-sectional area of 1 mm2), which were tested under tension in a universal testing machine. Additional CAD and SD samples were prepared for scanning electron microscopy observations. Additional and extended etching significantly increased µTBS to CAD; however, µTBS of both adhesives to CAD were significantly lower than to SD. Additional and extended etching can improve bonding to CAD; however, adhesives applied on SD showed the best results for bonding. [source]

Engraving of a Pd77.5Cu6Si16.5 Bulk Metallic Glass,

ADVANCED ENGINEERING MATERIALS, Issue 6 2007
G. Fiore
A bulk cylinder, 2 mm in diameter, of Pd77.5Cu6Si16.5 amorphous alloys has been obtained by copper moulding. Slices of the sample have been engraved in the undercooled liquid regime just above the glass transition reproducing details of a 5 Euro cent coin by suitably choosing the annealing procedure. The samples remained glassy after processing. [source]

Consumer acceptance and physicochemical quality of osmodehydrated carambola (Averrhoa carambola L.) slices

INTERNATIONAL JOURNAL OF CONSUMER STUDIES, Issue 1 2006
Lauren K. Maharaj
Abstract Consumer acceptability and physicochemical properties of candied osmodehydrated (OD) carambola were evaluated in an effort to increase consumption of the fruit. Fresh carambola slices (Averrhoa carambola L.) were soaked in either 15% or 20% or 25% NaCl brine for 18 h, then in 40 °Brix sucrose for 18 h, followed by 60 °Brix sucrose for 18 h and dried at 55°C for 18 h. Slices soaked in 15% NaCl were most preferred by a focus group. Decreases in pH, total soluble solids (TSS) and increase in salinity were recorded in sucrose solutions after soaking carambola. During osmosis, carambola became less green and more orange-yellow (P < 0.05). The pH of candied OD products varied between 3.62 and 4.16, salinity 16,28 ppt, TSS of 67,70 °Brix and 18.8,20.8% moisture. There were no differences (P > 0.05) in flavour or texture for products brined in 15% NaCl and soaked in either refined white sucrose (RS) solutions or brown unrefined sucrose (URS) syrups, but differences (P < 0.05) in appearance, colour and overall acceptability. Candied RS carambola had higher (P < 0.05) overall acceptability (6.2 , liked slightly to moderately) to products from URS (5.6 , neither liked nor disliked to liked slightly), which was also supported by paired preference testing. Flavour was most liked (P < 0.05) of all sensory attributes. [source]

Effects of heat treatment on the quality of fresh-cut Chinese water chestnut

INTERNATIONAL JOURNAL OF FOOD SCIENCE & TECHNOLOGY, Issue 2 2004
Litao Peng
Summary A heat treatment to inhibit browning and maintain the quality of fresh-cut Chinese water chestnut was developed. Slices of Chinese water chestnut, cv. Guilin, were immersed in boiling water for 30 s, placed into film-wrapped trays and then stored at 4 °C for up to 12 days. Changes in browning, eating quality and disease incidence were measured. The effect of heat treatment on the content of total phenolics and activities of phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) was also evaluated. The heat treatment effectively prevented browning associated with PAL, PPO and POD activities and total phenolic content and delayed the decrease in eating quality, which is associated with reduced total soluble solids, titratable acidity and ascorbic acid, compared with fresh-cut Chinese water chestnut. Inhibition of browning by heat treatment without microbial growth was achieved for 12 days of storage at 4 °C. These results showed that heat treatment effectively maintained the quality of fresh-cut Chinese water chestnut. [source]

Nucleolar organizer region staining patterns in paraffin-embedded tissue cells from human skin cancers

JOURNAL OF CUTANEOUS PATHOLOGY, Issue 5 2005
Rosana F. Romão-Corrêa
Background:, Increased number of nucleoli (nucleolar organizer regions, NORs) with abnormal shapes and sizes, including small dots, has been used as prognostic tools to evaluate tumor proliferation levels and troublesome borderline lesions. In this study, NOR patterns of skin cancers were performed in the search of a valuable prognostic method to complement other histological procedures. Methods:, Paraffin-embedded tumor tissue was obtained from basal and squamous cell carcinomas, cutaneous malignant melanoma, premalignant lesions, and Skmel-28 human melanoma cells. Slices were dewaxed and AgNOR stained. The patterns were scored and submitted for statistical analyses. Results:, All types of cancer cells showed variable numbers of abnormally shaped nucleoli and dot-like structures. Only tumor cells presented four or more nucleoli, with or without dots, while 85% of the normal cells had one single NOR without dots. Most data were statistically significant when compared to normal cells. As a whole, squamous cell carcinoma and malignant melanoma tumor cells had less NOR alterations than basal cell carcinoma (BCC) tumor types. Conclusions:, Changes in the number and shape of nucleoli present in malignant cells could be attributed to increased levels on rDNA transcription on cancer cells, besides abnormal remodeling of chromatin, which could disrupt proper nucleoli association. Increased genetic alterations on malignant basal cells could contribute to impair invasive and migration abilities of BCC tumors. [source]

QUALITY OF FRESH-CUT TOMATOES

JOURNAL OF FOOD QUALITY, Issue 3 2004
ABDUL HAKIM
ABSTRACT Tomato fruit at pink or light-red stages of ripening were cut into slices, rinsed with water containing 50 ,L.L,1 sodium hypochlorite and drained. They were stored in perforated polyethylene package at 1C in air or controlled atmosphere for five and ten days. Weight loss, chlorophyll and lycopene contents, seed discoloration, fungal infection, total soluble solids content, titratable acidity, sugar/acid ratio, pH, flavor and taste, firmness, ascorbic acid content, rate of respiration and ethylene production, and electrolyte leakage were evaluated after each storage period. The overall quality of controlled atmosphere-stored slices was better than that of air stored slices. Slices from light-red fruit had lower weight loss, minimum seed discoloration, lower [source]

A Novel Method for Air Drying Aloe Leaf Slices by Covering with Filter Papers as a Shrink-Proof Layer

JOURNAL OF FOOD SCIENCE, Issue 9 2009
S.A. Kim
ABSTRACT:, To prevent the shrinkage of aloe vera slices during air drying, a method utilizing a shrink-proof layer was developed. The sample was configured of whole leaf aloe slices, where 1 side or both sides were covered with filter papers as shrink-proof layers. After air drying by varying the air temperature and the slice thickness, the drying characteristics, as well as several quality factors of the dried aloe vera leaf slices, were analyzed. In the simulation of the drying curves, the modified Page model showed the best fitness, representing a diffusion-controlled drying mechanism. Nonetheless, there was a trace of a constant-rate drying period in the samples dried by the method. Shrinkage was greatly reduced, and the rehydration ratios increased by approximately 50%. Scanning electron microscopic analysis revealed that the surface structure of original fibrous form was well sustained. FT-IR characteristics showed that the dried samples could sustain aloe polysaccharide acetylation. Furthermore, the functional properties of the dried slices including water holding capacity, swelling, and fat absorption capability were improved, and polysaccharide retention levels increased by 20% to 30%. Therefore, we concluded that application of shrink-proof layers on aloe slices provides a novel way to overcome the shrinkage problems commonly found in air drying, thereby improving their functional properties with less cost. Practical Application: This research article demonstrates a novel air drying method using shrink-proof layers to prevent the shrinkage of aloe slices. We analyzed extensively the characteristics of shrinkage mechanism and physical properties of aloe flesh gels in this drying system. We concluded that this method can be a beneficial means to retain the functional properties of dried aloe, and a potential alternative to freeze drying, which is still costly. [source]

Diazepam Promotes ATP Recovery and Prevents Cytochrome c Release in Hippocampal Slices After In Vitro Ischemia

JOURNAL OF NEUROCHEMISTRY, Issue 3 2000
Francesca Galeffi
Abstract: Benzodiazepines protect hippocampal neurons when administered within the first few hours after transient cerebral ischemia. Here, we examined the ability of diazepam to prevent early signals of cell injury (before cell death) after in vitro ischemia. Ischemia in vitro or in vivo causes a rapid depletion of ATP and the generation of cell death signals, such as the release of cytochrome c from mitochondria. Hippocampal slices from adult rats were subjected to 7 min of oxygen-glucose deprivation (OGD) and assessed histologically 3 h after reoxygenation. At this time, area CA1 neurons appeared viable, although slight abnormalities in structure were evident. Immediately following OGD, ATP levels in hippocampus were decreased by 70%, and they recovered partially over the next 3 h of reoxygenation. When diazepam was included in the reoxygenation buffer, ATP levels recovered completely by 3 h after OGD. The effects of diazepam were blocked by picrotoxin, indicating that the protection was mediated by an influx of Cl - through the GABAA receptor. It is interesting that the benzodiazepine antagonist flumazenil did not prevent the action of diazepam, as has been shown in other studies using the hippocampus. Two hours after OGD, the partial recovery of ATP levels occurred simultaneously with an increase of cytochrome c (,400%) in the cytosol. When diazepam was included in the reoxygenation buffer, it completely prevented the increase in cytosolic cytochrome c. Thus, complete recovery of ATP and prevention of cytochrome c release from mitochondria can be achieved when diazepam is given after the loss of ATP induced by OGD. [source]

Effects of Ethanol on Persistent Activity and Up-States in Excitatory and Inhibitory Neurons in Prefrontal Cortex

ALCOHOLISM, Issue 12 2009
John J. Woodward
Background:, Elucidating mechanisms that underlie the neural actions of ethanol is critical for understanding how this drug affects behavior. Increasing evidence suggests that, in addition to mid-brain dopaminergic regions, higher cortical structures play an important role in the pathophysiology associated with alcohol abuse. Previous studies from this laboratory used a novel slice co-culture system to demonstrate that ethanol reduces network-dependent patterns of activity in excitatory pyramidal neurons of the prefrontal cortex (PFC). In the present study, we examine the effect of ethanol on the activity of fast-spiking (FS) interneurons, a sub-population of neurons critically involved in shaping cortical activity. Methods:, Slices containing the dorsolateral PFC were prepared from neonatal C57 mice and maintained in culture. After 2 to 3 weeks in vitro, whole-cell patch-clamp electrophysiology was used to monitor spontaneous episodes of persistent activity in prelimbic PFC neurons. In some experiments, the use-dependent NMDA receptor blocker, MK801, was included in the pipette recording solution to assess the contribution of NMDA receptors to up-states. Results:, MK801 reduced up-state amplitude and revealed underlying fast EPSPs in excitatory pyramidal neurons while having little effect on these parameters in FS interneurons. Despite this difference, ethanol (44 mM), significantly reduced up-state duration and up-state area in both pyramidal and FS interneurons. Conclusions:, These results suggest that ethanol reduces the activity of FS interneurons due to disruption of network-dependent activity. This would be expected to further impair the ability of PFC networks to carry out their normal function and may contribute to the adverse effects of ethanol on PFC-dependent behaviors. [source]

Anatomical Features of the Carpal Flexor Retinaculum of the Horse

ANATOMIA, HISTOLOGIA, EMBRYOLOGIA, Issue 6 2008
A. Probst
Summary This study aims to elucidate the topographical anatomy of the carpal flexor retinaculum or palmar anular carpal ligament (PACL) in the horse. Ten specimen of the carpus of five healthy horses were studied by dissection in layers. Slices of 5 mm in thickness facilitated observation of the soft tissues. The superficial layer of the PACL subdivides into five compartments: one for the palmar nerve and the arterial and venous branches, one for only the radial artery, one for the radial vein, and one for the tendon of the radial carpal flexor muscle, and finally for the deep layer that supports all tendinous structures located palmar to the carpus, as well as the median artery and palmar medial nerve. The sections of the segmented PACL that are affected by carpal canal syndrome may vary with the aetiology of the space-occupying process. Precise anatomical knowledge of the structures may help in understanding the pathological processes and determining the most appropriate therapy. [source]

NEUTRON TOMOGRAPHY AS A VALUABLE TOOL FOR THE NON-DESTRUCTIVE ANALYSIS OF HISTORICAL BRONZE SCULPTURES

ARCHAEOMETRY, Issue 2 2010
E. H. LEHMANN
This paper explains, with the example of a Roman object (the ,Mars from Oberweningen', Switzerland), why neutrons can be used preferentially for the non-destructive analysis of metallic objects of relatively large size. This method is superior to the conventional X-ray tomography method due to the higher penetration ability in metals such as copper, tin and even lead. With this method, differing materials can also easily be differentiated. In this way, the inner and outer structures of objects can be observed with the help of tools based on mathematical algorithms. Slices at arbitrary positions and segmentation of different material clusters aid the understanding of manufacturing processes and can describe the present preservation status. This helps in selecting optimal and additional conservation measures in museums for further preservation. Beside the qualitative overview on structure and inner properties of the objects, quantitative information can be derived about material content and composition. This method and the subsequently needed facilities for this method are available at PSI for similar studies on demand. [source]

DTI in Context: Illustrating Brain Fiber Tracts In Situ

COMPUTER GRAPHICS FORUM, Issue 3 2010
Pjotr Svetachov
Abstract We present an interactive illustrative visualization method inspired by traditional pen-and-ink illustration styles. Specifically, we explore how to provide context around DTI fiber tracts in the form of surfaces of the brain, the skull, or other objects such as tumors. These contextual surfaces are derived from either segmentation data or generated using interactive iso-surface extraction and are rendered with a flexible, slice-based hatching technique, controlled with ambient occlusion. This technique allows us to produce a consistent and frame-coherent appearance with precise control over the lines. In addition, we provide context through cutting planes onto which we render gray matter with stippling. Together, our methods not only facilitate the interactive exploration and illustration of brain fibers within their anatomical context but also allow us to produce high-quality images for print reproduction. We provide evidence for the success of our approach with an informal evaluation with domain experts. [source]

Out-of-Core and Dynamic Programming for Data Distribution on a Volume Visualization Cluster

COMPUTER GRAPHICS FORUM, Issue 1 2009
S. Frank
I.3.2 [Computer Graphics]: Distributed/network graphics; C.2.4 [Distributed Systems]: Distributed applications Abstract Ray directed volume-rendering algorithms are well suited for parallel implementation in a distributed cluster environment. For distributed ray casting, the scene must be partitioned between nodes for good load balancing, and a strict view-dependent priority order is required for image composition. In this paper, we define the load balanced network distribution (LBND) problem and map it to the NP-complete precedence constrained job-shop scheduling problem. We introduce a kd-tree solution and a dynamic programming solution. To process a massive data set, either a parallel or an out-of-core approach is required. Parallel preprocessing is performed by render nodes on data, which are allocated using a static data structure. Volumetric data sets often contain a large portion of voxels that will never be rendered, or empty space. Parallel preprocessing fails to take advantage of this. Our slab-projection slice, introduced in this paper, tracks empty space across consecutive slices of data to reduce the amount of data distributed and rendered. It is used to facilitate out-of-core bricking and kd-tree partitioning. Load balancing using each of our approaches is compared with traditional methods using several segmented regions of the Visible Korean data set. [source]

DiFi: Fast 3D Distance Field Computation Using Graphics Hardware

COMPUTER GRAPHICS FORUM, Issue 3 2004
Avneesh Sud
We present an algorithm for fast computation of discretized 3D distance fields using graphics hardware. Given a set of primitives and a distance metric, our algorithm computes the distance field for each slice of a uniform spatial grid baly rasterizing the distance functions of the primitives. We compute bounds on the spatial extent of the Voronoi region of each primitive. These bounds are used to cull and clamp the distance functions rendered for each slice. Our algorithm is applicable to all geometric models and does not make any assumptions about connectivity or a manifold representation. We have used our algorithm to compute distance fields of large models composed of tens of thousands of primitives on high resolution grids. Moreover, we demonstrate its application to medial axis evaluation and proximity computations. As compared to earlier approaches, we are able to achieve an order of magnitude improvement in the running time. Categories and Subject Descriptors (according to ACM CCS): I.3.3 [Computer Graphics]: Distance fields, Voronoi regions, graphics hardware, proximity computations [source]

Out-of-core compression and decompression of large n -dimensional scalar fields

COMPUTER GRAPHICS FORUM, Issue 3 2003
Lawrence Ibarria
We present a simple method for compressing very large and regularly sampled scalar fields. Our method is particularlyattractive when the entire data set does not fit in memory and when the sampling rate is high relative to thefeature size of the scalar field in all dimensions. Although we report results foranddata sets, the proposedapproach may be applied to higher dimensions. The method is based on the new Lorenzo predictor, introducedhere, which estimates the value of the scalar field at each sample from the values at processed neighbors. The predictedvalues are exact when the n-dimensional scalar field is an implicit polynomial of degreen, 1. Surprisingly,when the residuals (differences between the actual and predicted values) are encoded using arithmetic coding,the proposed method often outperforms wavelet compression in anL,sense. The proposed approach may beused both for lossy and lossless compression and is well suited for out-of-core compression and decompression,because a trivial implementation, which sweeps through the data set reading it once, requires maintaining only asmall buffer in core memory, whose size barely exceeds a single (n,1)- dimensional slice of the data. Categories and Subject Descriptors (according to ACM CCS): I.3.5 [Computer Graphics]: Compression, scalar fields,out-of-core. [source]

Reparallelization techniques for migrating OpenMP codes in computational grids

CONCURRENCY AND COMPUTATION: PRACTICE & EXPERIENCE, Issue 3 2009
Michael Klemm
Typical computational grid users target only a single cluster and have to estimate the runtime of their jobs. Job schedulers prefer short-running jobs to maintain a high system utilization. If the user underestimates the runtime, premature termination causes computation loss; overestimation is penalized by long queue times. As a solution, we present an automatic reparallelization and migration of OpenMP applications. A reparallelization is dynamically computed for an OpenMP work distribution when the number of CPUs changes. The application can be migrated between clusters when an allocated time slice is exceeded. Migration is based on a coordinated, heterogeneous checkpointing algorithm. Both reparallelization and migration enable the user to freely use computing time at more than a single point of the grid. Our demo applications successfully adapt to the changed CPU setting and smoothly migrate between, for example, clusters in Erlangen, Germany, and Amsterdam, the Netherlands, that use different kinds and numbers of processors. Benchmarks show that reparallelization and migration impose average overheads of about 4 and 2%, respectively. Copyright © 2008 John Wiley & Sons, Ltd. [source]