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Selected AbstractsVariability in pain response to a non-pharmacological intervention across repeated routine pain exposure in preterm infants: a feasibility studyACTA PAEDIATRICA, Issue 5 2009E Cignacco Abstract Aim: To explore the variability in pain response in preterm infants across time who received sucrose during routine heel stick. Method: Single group, exploratory repeated measures design. Setting: Two tertiary level neonatal intensive care units (NICU) in Switzerland. Subjects: Nine preterm infants born between 28 2/7 and 31 4/7 weeks of gestation during their first 14 days of life. Measurements: Pain was assessed by the Bernese Pain Scale for Neonates (BPSN), the Premature Infant Pain Profile (PIPP) and the Visual Analogue Scale (VAS). Salivary cortisol was analysed. Results: 72,94% of the variability was within-subject variability, indicating inconsistency of pain responses across the 5 heel sticks. Interrater agreement was highest during heel sticks 1,3 and decreased during heel stick 4 and 5, indicating a possible alteration of pain patterns. No significant differences in the amount of cortisol could be detected before and after the heel sticks (p = 0.55), indicating no stress-induced peak after the painful intervention. However, a general gradual decrease of cortisol levels across time could be detected. Conclusion: A high variability in pain response among preterm neonates across time could be described. Consistency of cortisol levels before and after the heel sticks could indicate the effectiveness of sucrose across time. [source] A novel exocrine structure of the bicellular unit type in the thorax of termitesACTA ZOOLOGICA, Issue 2 2010Teresa Telles Gonçalves Abstract Gonçalves, T. T., DeSouza, O. and Billen, J. 2010. A novel exocrine structure of the bicellular unit type in the thorax of termites , Acta Zoologica (Stockholm) 91: 193,198 Studying the thorax of some Termitidae species, we found two pairs of hitherto unknown lateral glands in the mesothorax and metathorax of both workers and soldiers. The glands consist of distinct clusters of class 3 secretory cells accompanied by their duct cells, located in the upper lateral portion of the thoracic wall. Ultrastructural observations reveal numerous mitochondria, a well-developed Golgi apparatus and vesicular smooth endoplasmic reticulum, indicating a cytoplasm with intensive metabolic activity. The gland is reported to occur in Microcerotermes strunckii, Cornitermes cumulans and Nasutitermes minor, three species comprising an interesting morpho-behavioural gradient, respectively, from only mechanical, through mechanical,chemical, to only chemical defence systems. The extent of such a gradient allows speculations that this gland would be related to the general needs of termites, rather than to some specificities of a single group. We warn, however, that complementary studies are needed, before any conclusions can be drawn. [source] Ant versus bird exclusion effects on the arthropod assemblage of an organic citrus groveECOLOGICAL ENTOMOLOGY, Issue 3 2010JOSEP PIÑOL 1. Predation-exclusion experiments have highlighted that top-down control is pervasive in terrestrial communities, but most of these experiments are simplistic in that they only excluded a single group of predators and the effect of removal was evaluated on a few species from the community. The main goal of our study was to experimentally establish the relative effects of ants and birds on the same arthropod assemblage of canopy trees. 2. We conducted 1-year long manipulative experiments in an organic citrus grove intended to quantify the independent effects of bird and ant predators on the abundance of arthropods. Birds were excluded with plastic nets whereas ants were excluded with sticky barriers on the trunks. The sticky barrier also excluded other ground dwelling insects, like the European earwig Forficula auricularia L. 3. Both the exclusion of ants and birds affected the arthropod community of the citrus canopies, but the exclusion of ants was far more important than the exclusion of birds. Indeed, almost all groups of arthropods had higher abundance in ant-excluded than in control trees, whereas only dermapterans were more abundant in bird-excluded than in control trees. A more detailed analysis conducted on spiders also showed that the effect of ant exclusion was limited to a few families rather than being widespread over the entire diverse spectrum of spiders. 4. Our results suggest that the relative importance of vertebrate and invertebrate predators in regulating arthropod populations largely depends on the nature of the predator,prey system. [source] Jumping off Arnstein's ladder: social learning as a new policy paradigm for climate change adaptationENVIRONMENTAL POLICY AND GOVERNANCE, Issue 6 2009Kevin Collins Abstract Participation of citizens, groups, organizations and businesses is now an essential element to tackle climate change effectively at international, European Union, national and local levels. However, beyond the general imperative to participate, major policy bodies offer little guidance on what this entails. We suggest that the dominance of Arnstein's ladder of citizen participation in policy discourses constrains the ways we think about, and critically the purposes we ascribe to, participation in a climate change context. We suggest an alternative framing of climate change, where no single group has clear access to understanding the issue and its resolution. Thus adaptation is fundamentally dependent on new forms of learning. Drawing on experiences of social learning approaches to natural resource managing, we explore how a commitment to social learning more accurately embodies the new kinds of role, relationship, practice and sense of purpose required to progress adaptive climate change agendas and practices. Copyright © 2009 John Wiley & Sons, Ltd and ERP Environment. [source] Richness and diversity of helminth communities in tropical freshwater fishes: empirical evidenceJOURNAL OF BIOGEOGRAPHY, Issue 4 2000Anindo Choudhury Abstract Aim Published information on the richness and diversity of helminth parasite communities in tropical freshwater fishes is reviewed in response to expectations of species-rich parasite communities in tropical regions. Location Areas covered include the tropics and some subtropical areas. In addition, the north temperate area of the nearctic zone is included for comparison. Methods Data from 159 communities in 118 species of tropical freshwater fish, summarized from 46 published studies, were used for this review. Parasite community descriptors used in the analyses included component community richness and calculated mean species richness. Data from 130 communities in 47 species of nearctic north temperate freshwater fish were summarized from 31 studies and used for comparison. Results The component helminth communities of many tropical freshwater fish are species-poor, and considerable proportions of fish from certain parts of the tropics, e.g. West African drainages, are uninfected or lightly infected. Mean helminth species richness was low and equaled or exceeded 2.0 in only 22 of 114 communities. No single group of helminths was identified as a dominant component of the fauna and species composition was variable among and within broader geographical areas. The richest enteric helminth assemblages were found in mochokid and clariid catfish with a mixed carnivorous diet, whereas algal feeders, herbivores and detritivores generally had species-poor gut helminth communities. Comparisons indicated that certain areas in the north temperate region had higher helminth species richness in fishes than areas in the tropics. Main conclusions Expectations of high species richness in helminth communities of tropical freshwater fishes are not fulfilled by the data. Direct comparisons of infracommunities and component communities in host species across widely separated phylogenetic and geographical lines are inappropriate. Examination of latitudinal differences in richness of monophyletic parasite groups or of compound communities may uncover patterns different from those found in this study. Richness of helminth communities may be ultimately determined not by the number of host species present but by the degree of divergence of host lineages and by their diversification modes. A phylogenetic framework for hosts and parasites will reveal if increased host species richness within host clades, when host speciation is accompanied by habitat or diet specialization, or both, leads to lower helminth diversity in host species by fragmentation of a core helminth fauna characteristic or specific of the larger host clade. This pattern may be analysed in the context of cospeciation and acquisition from other unrelated hosts (host-sharing or host-switching). [source] Pharmacokinetics and tolerability of modafinil tablets in Chinese subjectsJOURNAL OF CLINICAL PHARMACY & THERAPEUTICS, Issue 4 2008P. Xu PhD Summary Objective:, To investigate the pharmacokinetics and tolerability of modafinil in Chinese subjects. Methods:, Twelve healthy volunteers were given an escalating single dose of modafinil (100, 200 and 400 mg) in a three-period study (study 1). Another 12 volunteers received 100 mg twice daily for 7 days in multiple-dose study (study 2). Blood samples were taken from 0 to 60 h for study 1. And samples for study 2 were collected before administration on three consecutive morning and then from 0 to 60 h after the last dose. Pharmacokinetic parameters were calculated and compared with results from published data. Results:, In study 1, Cmax and area under the concentration,time curve of modafinil and modafinil acid were increased proportionally with dose levels; t1/2 was independent on the dose levels. In study 2, the steady state was reached on day 4, and mean trough plasma concentration of modafinil was 1·36 ± 0·34 ,g/mL. Apparent plasma clearance and apparent volume of distribution were lower in 100 mg twice-daily group than those in 100 mg single group. The adverse events were mild and moderate in study 1 and 2. Conclusions:, In this pharmacokinetic study, modafinil was safe and well tolerated by young healthy Chinese subjects. The major pharmacokinetic parameters of modafinil in Chinese subjects are similar to those reported in Caucasians although the half-life seems to be longer in the former than in the latter. This apparent difference requires investigation. [source] cDNA cloning of the housefly pigment-dispersing factor (PDF) precursor protein and its peptide comparison among the insect circadian neuropeptidesJOURNAL OF PEPTIDE SCIENCE, Issue 2 2004Ayami Matsushima Abstract Pigment-dispersing factor (PDF), an 18-amino acid neuropeptide, is a principal circadian neurotransmitter for the circadian rhythms of the locomotor activity in flies. Recently, two completely different types of PDF precursor were clarified; that of the cricket Gryllus bimaculatus and that of the last-summer cicada Meimuna opalifera. The G. bimaculatus PDF precursor is extraordinarily short and comprises a nuclear localization signal (NLS), while the M. opalifera PDF precursor is of ordinary length, comparable to that seen for the precursors of crustacean ,-PDH homologues. Although their PDF peptide regions were exactly the same, the regions containing a signal peptide combined with a PDF-associated peptide (PAP) were remarkably different from each other. Such a grouping suggested a fundamental role for the PAP peptide in the circadian clock, perhaps associated with PDF function. In the present study, the cDNA cloning of PDF from the adult brains of the housefly Musca domestica was carried out and it was found that an isolated clone (527 bp) encodes a PDF precursor protein of ordinary length. The PDF peptide shows a high sequence identity (78%,94%) and similarity (89%,100%) to insect PDFs and also to the crustacean ,-PDH peptides. In particular, there is only a single amino acid difference between the PDFs of Musca and Drosophila; at position 14 Ser for Musca PDF and Asn for Drosophila PDF. A characteristic Ser10 in Drosophila was retained in Musca, indicating the presence of a structural profile unique to these PDFs. The results of sequence analyses suggest that Musca and Drosophila PDFs are to be considered members of a single group that has evolved structurally. When the primary structure of the PAP regions was compared, the Musca PDF precursor also belonged to the same group as that to which the Drosophila PDF precursor belongs. Copyright © 2003 European Peptide Society and John Wiley & Sons, Ltd. [source] The use of ribotyping and antibiotic resistance patterns for identification of host sources of Escherichia coli strainsLETTERS IN APPLIED MICROBIOLOGY, Issue 1 2005M. Samadpour Abstract Aims:, To compare antibiotic resistance and ribotyping patterns ability to identify triplicate isolates sent from a group of 40 Escherichia coli taken from seven host sources. Methods and Results:, Of the 120 isolates, 22 isolates were resistant to ampicillin, streptomycin, tetracycline and trimethoprim and 98 isolates were susceptible. Antibiotic patterns identified 33 of the triplicates and three of the six groups had isolates from multiple hosts. Ribotyping divided the isolates into 27 ribotype groups with all triplicates grouped into the same ribotype group with one host per group. Conclusions:, Antibiotic susceptibility pattern placed 98 of the isolates in a single group with 50% of the antibiotic susceptibility pattern groups containing multiple host species. Ribotyping groups were host specific with each host having one to seven ribotype groups. Significance and Impact of the Study:, Antibiotic susceptibility pattern groups have been used for environmental source identification and faecal pollution tracking, however these groups do not always distinguish between host species. Stability of the markers is a potential concern and this system can only be used if antibiotic resistance levels are high in the isolates studied. All isolates have a ribotype group which was stable and like other molecular methods has advantages over antibiotic susceptibility pattern groups which uses a phenotypic method. [source] Hydroid assemblages from the Southwestern Atlantic Ocean (34,42° S)MARINE ECOLOGY, Issue 1 2009Gabriel N. Genzano Abstract This paper provides updated taxonomic knowledge about hydrozoan species and provides ecological information including geographical and bathymetric distributions and biological substrata for the various hydroid assemblages from the Sub-Antarctic Biogeographical Region, mainly Buenos Aires and the Uruguayan coasts. Five of the 41 species found are new records for the study region. Thirty-one species (75.6%), all found at depths of less than 80 m, have cosmopolitan distributions. Biodiversity decreased markedly below 80 m depth, where nine species (,22%) distributed in the Southern hemisphere and one endemic species (2.4%) were found. Most species were non-specific epizoites, occurring on diverse substrata. A non-parametric multivariate similarity analysis revealed nine species groups that were correlated with large-scale and local oceanographic patterns and with the availability of suitable substrata. The main hydroid substrata for eight of the groups were cnidarians, molluscs (mainly blue mussels), quartzite rocks and sponges. In a single group found in Patagonian scallop beds, the main biological substrata were polychaete tubes, other hydroids and scallops. [source] Evidence of genetic distinction and long-term population decline in wolves (Canis lupus) in the Italian ApenninesMOLECULAR ECOLOGY, Issue 3 2004V. Lucchini Abstract Historical information suggests the occurrence of an extensive human-caused contraction in the distribution range of wolves (Canis lupus) during the last few centuries in Europe. Wolves disappeared from the Alps in the 1920s, and thereafter continued to decline in peninsular Italy until the 1970s, when approximately 100 individuals survived, isolated in the central Apennines. In this study we performed a coalescent analysis of multilocus DNA markers to infer patterns and timing of historical population changes in wolves surviving in the Apennines. This population showed a unique mitochondrial DNA control-region haplotype, the absence of private alleles and lower heterozygosity at microsatellite loci, as compared to other wolf populations. Multivariate, clustering and Bayesian assignment procedures consistently assigned all the wolf genotypes sampled in Italy to a single group, supporting their genetic distinction. Bottleneck tests showed evidences of population decline in the Italian wolves, but not in other populations. Results of a Bayesian coalescent model indicate that wolves in Italy underwent a 100- to 1000-fold population contraction over the past 2000,10 000 years. The population decline was stronger and longer in peninsular Italy than elsewhere in Europe, suggesting that wolves have apparently been genetically isolated for thousands of generations south of the Alps. Ice caps covering the Alps at the Last Glacial Maximum (c. 18 000 years before present), and the wide expansion of the Po River, which cut the alluvial plains throughout the Holocene, might have provided effective geographical barriers to wolf dispersal. More recently, the admixture of Alpine and Apennine wolf populations could have been prevented by deforestation, which was already widespread in the fifteenth century in northern Italy. This study suggests that, despite the high potential rates of dispersal and gene flow, local wolf populations may not have mixed for long periods of time. [source] Structured review of enamel erosion literature (1980,1998): a critical appraisal of experimental, clinical and review publicationsORAL DISEASES, Issue 4 2000G. Maupome OBJECTIVE: To attain an objective account of the methods to measure enamel erosion used in 1980,1998 publications, a structured review of the literature was undertaken. METHODS: Inclusion and exclusion criteria were applied to 731 clinical/experimental research and review reportS. Eighty-five included papers were subsequently rated according to ,hierarchy of evidence' guidelines to assess the strength of the report's design and the relevance of the evidence to replicating enamel erosion in vivo in humanS. Scores were assigned to rate each aspect in the guidelines. RESULTS: A total of 16 clinical, 13 review and 56 experimental papers were assessed; 36.4% were published during 1996,1998.Excluding reviews, 16 papers were qualitative and 56 quantitative; 51 used human enamel. Our classification yielded nine groups of methods (five scoring systems and 26 measurement techniques).CTFPHE (Can Med Assoc J 1992; 147: 443) grading of research reports indicated that 2.8% provided evidence grade I; 20.8%, grade IIa; 63.9%, grade III; and 12.5%, grade IV. CONCLUSIONS: There has been a consistent increase in the body of knowledge. The overall quality of publications has not substantially changed over time. Experimental studies were more often quantitative, and quantitative studies had better research designS. No single group of research methods had obviously superior research designs. [source] The Asian birth outcome gapPAEDIATRIC & PERINATAL EPIDEMIOLOGY, Issue 4 2006Cheng Qin Summary Asians are often considered a single group in epidemiological research. This study examines the extent of differences in maternal risks and birth outcomes for six Asian subgroups. Using linked birth/infant death certificate data from the State of California for the years 1992,97, we assessed maternal socio-economic risks and their effect on birthweight, preterm delivery (PTD), neonatal, post-neonatal and infant mortality for Filipino (87 120), Chinese (67 228), Vietnamese (45 237), Korean (23 431), Cambodian/Laotian (21 239) and Japanese (18 276) live singleton births. The analysis also included information about non-Hispanic whites and non-Hispanic blacks in order to give a sense of the magnitude of risks among Asians. Logistic regression models explored the effect of maternal risk factors and PTD on Asian subgroup differences in neonatal and post-neonatal mortality, using Japanese as the reference group. Across Asian subgroups, the differences ranged from 2.5- to 135-fold for maternal risks, and 2.2-fold for infant mortality rate. PTD was an important contributor to neonatal mortality differences. Maternal risk factors contributed to the disparities in post-neonatal mortality. Significant differences in perinatal health across Asian subgroups deserve ethnicity-specific interventions addressing PTD, teen pregnancy, maternal education, parity and access to prenatal care. [source] Educating general practitioners about prenatal testing: approaches and challengesPRENATAL DIAGNOSIS, Issue 7 2005Sylvia Metcalfe Abstract Objectives To examine the effects of an education intervention on the knowledge and practice behaviours of general practitioners (GPs) regarding prenatal screening and diagnostic testing in Melbourne, Australia. Methods A single group, pre-test, repeated post-test design was used. Information on demographics, knowledge, behaviours and skills was collected via a self-administered questionnaire prior to the educational intervention. Responses to individual questions were coded and scores calculated, as well as a percent total score. Following the education, data were again collected, immediately afterwards (Post-Q) and six-to-eight months later (Foll-Q). Results Data for all three time points were collected from 63 GPs and showed a variable, and relatively poor knowledge regarding aspects of prenatal testing, especially before education. The percent mean total score at baseline was 51.2 ± 1.59% (CI 48.02 to 54.39), which increased significantly (p < 0.001) in both Post-Q (62.88 ± 1.51%; CI 59.86 to 65.89) and Foll-Q (58.92 ± 1.6%; CI 55.71 to 62.12). Conclusion The educational intervention significantly increased knowledge and practice behaviour of GPs, even up to eight months later, but this could be improved further, to a considerable extent. There is a need for ongoing multi-faceted approaches to educating GPs on prenatal testing to ensure that they are engaged in appropriate practice. Copyright © 2005 John Wiley & Sons, Ltd. [source] Sources of protein in two semi-arid zone mistletoe specialists: Insights from stable isotopesAUSTRAL ECOLOGY, Issue 7 2009LAURENCE P. BAREA Abstract Obtaining adequate levels of dietary protein is essential for the physiology of consumers. This presents potential problems for frugivorous birds because fruit is generally low in protein rendering it nutritionally inadequate and potentially explaining the rarity of exclusive frugivory in birds. We addressed this issue by determining the isotope composition (15N/14N) in the whole blood of two mistletoe consumers, that is, painted honeyeater (Grantiella picta, Meliphagidae) and mistletoebird (Dicaeum hirundinaceum, Dicaeidae) during the grey mistletoe (Amyema quandang, Loranthaceae) fruiting peak in a semi-arid woodland, NSW, Australia. Grey mistletoe fruit pulp and arthropods were isotopically distinct (mean ,15N fruit 4.4,vs. arthropods 7.1,), thus readily discriminated using the stable isotope approach. Painted honeyeaters and mistletoebirds formed a single group based on their mean ,15N values and, on average, assimilated approximately half of their nitrogen from mistletoe fruit although individual variation was high. The importance of nitrogen derived from mistletoe fruit did not track its abundance in the environment, suggesting that at least during peak fruiting, this resource is not limiting at this site. Researchers should account for intraspecific variation and take a cautious approach when reconstructing diets using stable isotopes by incorporating individual-based analyses rather than presenting mean values alone. This is the first study to use the isotope approach to investigate the dietary relationship of mistletoe frugivores and mistletoe fruit and has implications for our understanding of the nutritional ecology of frugivores and its functional relationship to ecosystem processes such as seed dispersal. [source] | ||||||||||||||||