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Silage
Kinds of Silage Terms modified by Silage Selected AbstractsMicrobial Community Dynamics of a Continuous Mesophilic Anaerobic Biogas Digester Fed with Sugar Beet SilageENGINEERING IN LIFE SCIENCES (ELECTRONIC), Issue 4 2008B. Demirel Abstract The aim of the study was to investigate the long-term fermentation of an extremely sour substrate without any addition of manure. In the future, the limitation of manure and therefore the anaerobic digestion of silage with a very low buffering capacity will be an increasing general bottleneck for energy production from renewable biomass. During the mesophilic anaerobic digestion of sugar beet silage (without top and leaves) as the sole substrate (without any addition of manure), which had an extreme low pH of around 3.3, the highest specific gas production rate (spec. GPR) of 0.72,L/g volatile solids (VS),d was achieved at a hydraulic retention time (HRT) of 25,days compared to an organic loading rate (OLR) of 3.97,g VS/L,d at a pH of around 6.80. The methane (CH4) content of the digester ranged between 58 and 67,%, with an average of 63,%. The use of a new charge of substrate (a new harvest of the same substrate) with higher phosphate content improved the performance of the biogas digester significantly. The change of the substrate charge also seemed to affect the methanogenic population dynamics positively, thus improving the reactor performance. Using a new substrate charge, a further decrease in the HRT from 25 to 15,days did not influence the digester performance and did not seem to affect the structure of the methanogenic population significantly. However, a decrease in the HRT affected the size of the methanogenic population adversely. The lower spec. GPR of 0.54,L/g,VS,d attained on day,15 of the HRT could be attributed to a lower size of methanogenic population present in the anaerobic digester during this stage of the process. Furthermore, since sugar beet silage is a relatively poor substrate, in terms of the buffering capacity and the availability of nutrients, an external supply of buffering agents and nutrients is a prerequisite for a safe and stable digester operation. [source] Influences of Northern Leaf Blight on corn silage fermentation quality, nutritive value and feed intake by sheepANIMAL SCIENCE JOURNAL, Issue 4 2010Peng WANG ABSTRACT This study examined the differences between non-inoculated (control) corn and Northern Leaf Blight (NLB)-damaged corn (inoculated corn); dry matter (DM) yield, silage fermentation quality, nutritive value and feed intake by sheep were compared. Leaf, stem and grain dry weights and gross yield of inoculated corn were significantly (P < 0.05) decreased compared with control corn. The contents of water-soluble carbohydrate and nitrogen-free extract (NFE) were decreased in inoculated corn compared with control corn. Silage made from both inoculated and control corn showed good fermentation quality. The digestibility of DM, organic matter, ether extract, NFE, and energy of silage made from inoculated corn were significantly (P < 0.05) lower, and contents of total digestible nutrients (TDN) and digestible energy (DE) were also significantly (P < 0.05) lower compared with silage made from the control corn. DM intake showed no significant discrepancy between the two types of silage. TDN and DE intakes from inoculated silage were significantly (P < 0.05) lower compared with control silage. From the above results it was shown that NLB caused a decrease in DM yield and NFE content in corn and a decrease in the nutritive value and feed intake of silage. [source] Clinical acidosis in a Gippsland dairy herdAUSTRALIAN VETERINARY JOURNAL, Issue 6 2005E. BRAMLEY Objective: To report on spontaneous clinical and subclinical acidosis in a large dairy herd, to evaluate the diets and feeding strategies involved, and to report on measures of rumen function in the cows affected. Design: A Gippsland dairy herd was sampled as part of a wider randomised cross-sectional study that examined the prevalence, risk factors for, and effects of, acidosis on rumen function of dairy cattle. Three herds on the farm were involved in the study: the transition herd (cows 3 weeks prior to calving), the very fresh lactating herd (1 Fermentation characteristics and aerobic stability of grass silage inoculated with Lactobacillus buchneri, with or without homofermentative lactic acid bacteriaGRASS & FORAGE SCIENCE, Issue 4 2001F. Driehuis Aerobic spoilage by yeasts and moulds is a major cause of reduced nutritional value of silage and increases the risk of potential pathogenic microorganisms. Recent studies have shown that inoculation with Lactobacillus buchneri inhibits yeast growth and reduces the susceptibility to aerobic spoilage of various ensiled forages. The aim of this study was to determine whether these effects are retained when L. buchneri is added in combination with homofermentative lactic acid bacteria. In three experiments, silages were produced from perennial ryegrass [240,421 g kg,1 dry matter (DM)] inoculated with L. buchneri or L. buchneri plus a mixture of Pediococcus pentosaceus and Lactobacillus plantarum (inoculant PL). Uninoculated silage and silage inoculated with PL alone served as controls. Silages were examined for pH and DM loss in the course of ensilage and chemical and microbiological composition and aerobic stability after 3,4 months. L. buchneri plus PL and PL alone increased the initial rate of pH decline. L. buchneri alone and L. buchneri plus PL enhanced aerobic stability and, in general, reduced yeast and mould counts. In addition, these inoculants increased the final pH and DM loss and the concentrations of acetic acid and 1,2-propanediol (or propionic acid and 1-propanol instead of 1,2-propanediol), and decreased the concentration of lactic acid. The effects of L. buchneri on fermentation products increased with decreasing DM content. In silages of less than 270 g kg,1 DM, L. buchneri increased the ammonia-N concentration. It is suggested that this was associated with the relatively high final pH resulting from the high metabolic activity of L. buchneri in these silages. [source] Microbial Community Dynamics of a Continuous Mesophilic Anaerobic Biogas Digester Fed with Sugar Beet SilageENGINEERING IN LIFE SCIENCES (ELECTRONIC), Issue 4 2008B. Demirel Abstract The aim of the study was to investigate the long-term fermentation of an extremely sour substrate without any addition of manure. In the future, the limitation of manure and therefore the anaerobic digestion of silage with a very low buffering capacity will be an increasing general bottleneck for energy production from renewable biomass. During the mesophilic anaerobic digestion of sugar beet silage (without top and leaves) as the sole substrate (without any addition of manure), which had an extreme low pH of around 3.3, the highest specific gas production rate (spec. GPR) of 0.72,L/g volatile solids (VS),d was achieved at a hydraulic retention time (HRT) of 25,days compared to an organic loading rate (OLR) of 3.97,g VS/L,d at a pH of around 6.80. The methane (CH4) content of the digester ranged between 58 and 67,%, with an average of 63,%. The use of a new charge of substrate (a new harvest of the same substrate) with higher phosphate content improved the performance of the biogas digester significantly. The change of the substrate charge also seemed to affect the methanogenic population dynamics positively, thus improving the reactor performance. Using a new substrate charge, a further decrease in the HRT from 25 to 15,days did not influence the digester performance and did not seem to affect the structure of the methanogenic population significantly. However, a decrease in the HRT affected the size of the methanogenic population adversely. The lower spec. GPR of 0.54,L/g,VS,d attained on day,15 of the HRT could be attributed to a lower size of methanogenic population present in the anaerobic digester during this stage of the process. Furthermore, since sugar beet silage is a relatively poor substrate, in terms of the buffering capacity and the availability of nutrients, an external supply of buffering agents and nutrients is a prerequisite for a safe and stable digester operation. [source] Utilization of semi-natural grassland through integrated generation of solid fuel and biogas from biomass.GRASS & FORAGE SCIENCE, Issue 4 2009Abstract A procedure (Integrated Generation of Solid Fuel and Biogas from Biomass, IFBB) was developed which uses a screw press to separate the readily digestible constituents of mature grassland biomass into a press fluid for conversion into biogas and a fibrous press cake for processing into a solid fuel. Effects of mechanical dehydration and prior hydrothermal conditioning at different temperatures (5, 60 and 80°C) on concentrations of organic compounds in the press fluid and on methane production in batch experiments were evaluated for five semi-natural grasslands typical of mountain areas of Germany. Results show that the crude protein concentration of the press fluids was higher and crude fibre concentration was lower than that of the parent material (herbage conserved as silage). Digestion tests in batch fermenters showed that the methane yield of the press fluids was double [397,426 normal litre (NL) kg,1 volatile solids (VS) after 13 d] that of the whole-crop grassland silage (218 NL kg,1 VS after 27 d) but no consistent effect of higher temperature during conditioning was observed. Within 13 d of fermentation the decomposition of the organic matter (OM) that occurred in the press fluids was 0·90, whereas after 27 d of fermentation more than 0·40 of the OM remained undigested in the whole-crop silage, pointing at a marked reduction in retention time for anaerobic digestion of press fluids in continuous systems. Press fluids produced 0·90 of the maximum methane yield after 4 to 7 d compared with 19 days for the whole-crop silage. [source] Silage quality when Moringa oleifera is ensiled in mixtures with Elephant grass, sugar cane and molassesGRASS & FORAGE SCIENCE, Issue 4 2009B. Mendieta-Araica Abstract Fourteen different silages were prepared using mixtures of Moringa (Moringa oleifera), Elephant grass (Pennisetum purpureum cv Taiwan) or sugar cane (Saccharum officinarum). Molasses from sugar cane was used in the amounts of either 10 or 50 g kg,1 fresh matter (FM) in treatments without sugar cane. A completely randomized design with three replicates of each treatment was used. The silages were prepared in 1800 mL micro silos and opened after 120 d. The presence of Moringa and Elephant grass in the silage changed the pH by ,0·8 and +0·7, respectively (P < 0·001), indicating a favourable effect of Moringa on silage pH. Overall differences were found among treatments for dry matter content, crude protein and acetic acid concentrations, weight loss, CO2 production and silage pH after spoilage (P < 0·001). Weight loss was proportionately 0·034 and 0·014 in silages with and without sugar cane respectively (P < 0·001). Overall, differences (P < 0·05) were also found for neutral-detergent fibre and lactic acid concentrations, lactic acid bacteria counts, clostridial counts and time to spoilage of the silages. Treatments containing Moringa had higher lactic acid concentrations (+16 g kg,1 DM; P < 0·01) compared to treatments without but the presence of Moringa decreased time to spoilage by 67 h (P < 0·05). No differences were found in propionic acid concentration or fungal growth of the silages. It is concluded that Moringa can be used as a component of high quality silages which also contain high concentrations of crude protein. [source] Effects of different rates and timing of application of nitrogen as slurry and mineral fertilizer on yield of herbage and nitrate-leaching potential of a maize/Italian ryegrass cropping system in north-west PortugalGRASS & FORAGE SCIENCE, Issue 1 2009H. Trindade Abstract Efficient use of cattle-slurry to avoid nitrogen (N) leaching and other losses is important in designing intensive dairy systems to minimize pollution of air and water. The response in dry-matter (DM) yield of herbage and nitrate-leaching potential to different rates and timing of application of N as cattle slurry and/or mineral fertilizer in a double-cropping system producing maize (Zea mays L.) silage and Italian ryegrass (Lolium multiflorum Lam.) was investigated in north-west Portugal. Nine treatments with different rates and combinations of cattle slurry, and with or without mineral-N fertilizer, applied at sowing and as a top-dressing to both crops, were tested and measurements were made of DM yield of herbage, N concentration of herbage, uptake of N by herbage and amounts of residual soil nitrate-N to a depth of 1 m, in a 3-year experiment. Regression analysis showed that the application of 150 and 100 kg of available N ha,1 to maize and Italian ryegrass, respectively, resulted in 0·95 of maximum DM yields of herbage and 0·90 of maximum N uptake by herbage. Residual amounts of nitrate-N in soil after maize ranged from 48 to 278 kg N ha,1 with an exponential increase in response to the amount of N applied; there were higher values of nitrate-leaching potential when mineral-N fertilizer was applied. The results suggest that it is possible in highly productive maize/Italian ryegrass systems to obtain high DM yields of herbage for maize silage and Italian ryegrass herbage with minimal leaching losses by using slurry exclusively at annual rates of up to 250 kg available N ha,1 (equivalent to 480 kg total N ha,1) in three applications. [source] Dry matter production, nutritive value and efficiency of nutrient utilization of a complementary forage rotation compared to a grass pasture systemGRASS & FORAGE SCIENCE, Issue 3 2008S. C. Garcia Abstract In pasture-based dairy farming, new sustainable systems that involve the annual dry matter (DM) production of grazed and conserved forage beyond the potential of grazed pasture alone are being sought. The objective of this experiment conducted in Australia was to compare a complementary forage rotation (CFR) for conservation and grazing, comprising an annual sequence of three crops, namely maize (Zea mays L), forage rape (Brassica napus L) and a legume (Persian clover, Trifolium repesinatum L or maple pea, Pisum sativum L), with a pasture [kikuyu grass (Pennisetum clandestinum) over-sown with short-rotation ryegrass (Lolium multiflorum L)] as a pasture control treatment. The experiment was a complete randomized block design with four replicates (,0·7 ha each). Annual dry-matter (DM) yield over the 3 years averaged >42 t ha,1 year,1 for the CFR treatment and >17 t ha,1 year,1 for the pasture treatment. The high DM yield of the CFR treatment resulted from >27 t ha,1 year,1 from maize harvested for silage and >15 t DM ha,1 year,1 utilized by grazing the forage rape and legumes. Total input of nitrogen (N) and water were similar for both treatments, resulting in higher N- and water-use efficiency for the CFR treatment, which was more than twice that for the pasture treatment. Overall, the nutritive value of the pasture treatment was slightly higher than the mean for that of the CFR treatment. The implications of these results are that a highly productive system based on the CFR treatment in conjunction with the use of pasture is achievable. Such a dairy production system in Australia could increase the total supply of feed resources grown on-farm and the efficiency of use of key resources such as N and water. [source] The microbiological and chemical composition of silage over the course of fermentation in round bales relative to that of silage made from unchopped and precision-chopped herbage in laboratory silosGRASS & FORAGE SCIENCE, Issue 3 2008J. McEniry Abstract The composition of baled silage frequently differs from that of comparable conventional silage. A factorial experiment was conducted with three wilting treatments (0, 24 or 48 h) × three ensiling systems [unchopped grass in bales, unchopped grass in laboratory silos (LS), precision-chopped grass in LS] × six stages of ensiling to (i) confirm that the fermentation of unchopped grass in LS could be used as an adequate model for baled silage fermentation, (ii) quantify the differences between baled silage and silage made from precision-chopped herbage across a range of dry-matter contents and (c) quantify the fermentation dynamics within the various treatments. The onset of fermentation as evidenced by the accumulation of fermentation products and the decline in pH were slower (P < 0·05) in baled silage compared with silage made from precision-chopped herbage. Furthermore the pH (P < 0·001) and overall concentration of fermentation acids (P < 0·01) were lower while ammonia-N concentration was generally higher in baled silage, making it more conducive to the activities of Clostridia, Enterobacteria and yeast. Numbers of Enterobacteria were higher (P < 0·001) in baled silage in the early stages of ensilage and persisted in baled silage at the end of the storage period. The implications of a slower onset of fermentation in baled herbage are greater in farm practice, as the fermentation would be further restricted by a more extensive wilting of the herbage prior to ensiling. [source] Production and behavioural responses of high- and low-yielding dairy cows to different periods of access to grazing or to a maize silage and soyabean meal diet fed indoorsGRASS & FORAGE SCIENCE, Issue 4 2006O. Hernandez-Mendo Abstract The study examined whether high-yielding cows grazing pasture respond differently from low-yielding cows in milk production and feeding behaviour, to increasing the time made available for eating a maize silage and soyabean meal (TAMS) diet indoors and reducing the time available for grazing (TAG). Two experiments, each lasting 42 d, were carried out in spring (Experiment 1) and autumn (Experiment 2) using Holstein-Friesian cows at two different levels of milk yield (MYL). Milk production and feeding behaviour were examined for TAG + TAMS systems of 19 h, TAG plus 1 h TAMS (19 + 1), and 5 h TAG plus 15 h TAMS (5 + 15). There were two levels of concentrate (0 and 6 kg d,1), and in the spring experiment two sward heights (4,6 and 8,10 cm) were also studied. Milk yield, persistency of milk yield, liveweight change and estimated total DM intake were significantly higher on the 5 + 15 than on the 19 + 1 grazing system in Experiment 1 but not in Experiment 2. There were no significant interactions of TAG + TAMS treatment with MYL for any production or behavioural measurements except for maize silage feeding time, where high MYL cows spent a significantly greater time eating maize silage than low MYL cows on the 5 + 15 treatment but not on the 19 + 1 treatment. It can be concluded that high- and low-yielding cows respond similarly in milk production and feeding behaviour to different combinations of TAG and TAMS. In autumn, estimated daily intakes of herbage were lower on both grazing treatments relative to spring, resulting from lower rates of herbage intake with no compensatory increase in grazing time. In contrast, rates of intake of maize silage were higher in autumn especially on the 19 + 1 system. These results may imply a change of preference from herbage to maize silage between spring and autumn. [source] Effects of grass pasture and concentrate-based feeding systems for spring-calving dairy cows in early spring on performance during lactationGRASS & FORAGE SCIENCE, Issue 3 2005E. Kennedy Abstract The effect of offering a total mixed ration of silage and concentrate (proportionately 0·44 silage) system [indoor feeding system (IF)] was compared with grazing at a high daily herbage allowance with a low level of concentrate supplementation [early grazing system (EG)] in early spring on the performance of spring-calving dairy cows in Ireland. Sixty-four spring-calving Holstein,Friesian dairy cows (mean calving date, 2 February) were allocated to one of two systems between 16 February and 4 April 2004. An equal number of primiparous and multiparous cows were assigned to each system. The dairy cows on the IF system were housed for a 7-week period and offered a diet of 10·9 kg DM cow,1 d,1 (s.d. 2·3) of concentrate, the remainder of the diet was 8·6 kg DM cow,1 d,1 (s.d. 1·9) of grass silage. The dairy cows on the EG system were offered a mean daily herbage allowance of 15·1 kg DM cow,1 d,1 (s.d. 3·7) and were supplemented with 3·0 kg DM cow,1 d,1 (s.d. 1·0) of concentrate. There was no difference in milk yield between the two systems but the cows in the EG system had a higher milk protein concentration (2·9 g kg,1) and a higher milk protein yield than in the IF system. Milk fat concentration was higher for cows in the IF than EG system (3·0 g kg,1). There was no difference in total daily dry-matter intake between the systems, measured in week 6 of the study. Mean live weight of the cows in the IF system was greater than in the EG system. The results of the study suggest that a slightly greater performance can be achieved by a system offering a high daily herbage allowance to spring-calving dairy cows in early lactation compared with a system offering a total mixed ration containing a high proportion of concentrate with grass silage. [source] Fermentation patterns of small-bale silage and haylage produced as a feed for horsesGRASS & FORAGE SCIENCE, Issue 2 2005C. E. Müller Abstract The fermentation quality of small-bale silage and haylage for feeding to horses in Sweden, and using a conventional high-density hay baler, was investigated in two experiments. Treatments studied were use of additives (inoculants containing lactic acid bacteria and a chemical additive consisting of hexamethylenetetramine, sodium nitrate, sodium benzoate and sodium propionate), the influence of dry-matter (DM) content of wilted herbage and the effect of number of stretch film layers on fermentation pattern and aerobic stability. All silages and haylages were made from predominantly Timothy swards and were well fermented as indicated by low levels of ammonia and butyric acid. Values of pH were higher and concentrations of organic acids were lower in haylages than in the silages. This was not considered to be indicative of a poor fermentation in the haylage but of a restricted fermentation due to the high DM content of the herbage. The additives enhanced aerobic storage stability because of inhibition of mould growth. The only statistically significant effect of varying the number of stretch film layers was a higher content of CO2 inside the bales when ten layers of stretch film were applied compared with six layers. [source] Consequences for biodiversity of reducing inputs to upland temperate pastures: effects on beetles (Coleoptera) of cessation of nitrogen fertilizer application and reductions in stocking rates of sheepGRASS & FORAGE SCIENCE, Issue 2 2004P. Dennis Abstract Current policies for upland pasture management in the UK encourage the integration of environmental objectives with livestock production through extensification of grazing systems. This study tested the hypothesis that a greater sward height in the summer would increase the diversity and abundance of grassland beetles (Coleoptera) as has been demonstrated for insects of indigenous grasslands. The hypothesis was tested with an experiment on an upland sheep pasture in mid-Wales. Experimental treatments received different nitrogen fertilizer inputs (0 or 50 kg ha,1), sheep stocking densities (12 or 9 ewes ha,1) and average sward heights in summer were constrained to 3·5 or 5·5 cm by conserving surplus grass for silage in subplots. Five treatments, replicated in three randomized blocks, combined the two stocking densities and two sward heights without nitrogen fertilizer inputs, with the fifth combining the higher stocking density, shortest sward height and the nitrogen fertilizer input. Beetles were sampled with twelve pitfall traps in each of the fifteen plots from June to September in 1993 and 1995. In years 1 (1993) and 3 (1995) of the experiment, more Coleoptera species occurred in the tall sward (an average of nine species in addition to the forty-one species present in the sward with the conventional sward height). Continuously grazed as opposed to ensiled subplots supported more beetle species but fewer individuals. Species composition of ground (Carabidae) and rove (Staphylinidae) beetles varied between treatments more than the arithmetic differences in species number. The experimental results supported the hypothesis but the benefits of taller swards to species diversity were small in the sown pastures of the study compared with indigenous upland grasslands (c. 33% fewer species). Inheritance effects of drainage, fertilizer and lime inputs, and the different species and management of cultivated pastures, may constrain the conservation benefits of altered pasture management compared with indigenous grasslands. [source] Intake, liveweight gain and feed preference by steers fed combinations of lucerne and Westerwolds ryegrass silagesGRASS & FORAGE SCIENCE, Issue 1 2002E. Charmley Nutritive value and voluntary intake of legumes are generally considered to be higher than those of grasses when ensiled at similar digestibility, although high levels of soluble protein can result in low N utilization by animals and high losses to the environment. The objectives of this experiment were to describe the optimum combination of Westerwolds ryegrass (Lolium multiflorum Lam. cv. Aubade) and lucerne (Medicago sativa L. cv. AC Caribou) silages to maximize liveweight gain of steers fed silage, determine chemical components that are important and ascertain whether steers selected the optimum mixture when given a choice. Both silages contained similar concentrations of dry matter (DM), acid-detergent fibre (ADF) and organic acids, but lucerne silage had higher concentrations of N, soluble-N and ammonia-N. Westerwolds ryegrass silage contained more neutral-detergent fibre (NDF). In a 12-week experiment, voluntary intake by Hereford steers was not influenced when the proportion of the two silages was changed from 1 to 0 in 0·25 increments. However, liveweight gain and feed efficiency increased linearly (P < 0·001) as the proportion of ryegrass silage fed was increased. When preconditioned to either of the two silages, steers showed a significant preference for ryegrass over lucerne (P < 0·05). When conditioned to a mixture of both silages, no preference was elicited. It is suggested that extensive solubilization and deamination of protein in the lucerne silage may have caused the preference for Westerwolds ryegrass silage and the higher liveweight gains on diets containing higher proportions of Westerwolds ryegrass silage. [source] Fermentation characteristics and aerobic stability of grass silage inoculated with Lactobacillus buchneri, with or without homofermentative lactic acid bacteriaGRASS & FORAGE SCIENCE, Issue 4 2001F. Driehuis Aerobic spoilage by yeasts and moulds is a major cause of reduced nutritional value of silage and increases the risk of potential pathogenic microorganisms. Recent studies have shown that inoculation with Lactobacillus buchneri inhibits yeast growth and reduces the susceptibility to aerobic spoilage of various ensiled forages. The aim of this study was to determine whether these effects are retained when L. buchneri is added in combination with homofermentative lactic acid bacteria. In three experiments, silages were produced from perennial ryegrass [240,421 g kg,1 dry matter (DM)] inoculated with L. buchneri or L. buchneri plus a mixture of Pediococcus pentosaceus and Lactobacillus plantarum (inoculant PL). Uninoculated silage and silage inoculated with PL alone served as controls. Silages were examined for pH and DM loss in the course of ensilage and chemical and microbiological composition and aerobic stability after 3,4 months. L. buchneri plus PL and PL alone increased the initial rate of pH decline. L. buchneri alone and L. buchneri plus PL enhanced aerobic stability and, in general, reduced yeast and mould counts. In addition, these inoculants increased the final pH and DM loss and the concentrations of acetic acid and 1,2-propanediol (or propionic acid and 1-propanol instead of 1,2-propanediol), and decreased the concentration of lactic acid. The effects of L. buchneri on fermentation products increased with decreasing DM content. In silages of less than 270 g kg,1 DM, L. buchneri increased the ammonia-N concentration. It is suggested that this was associated with the relatively high final pH resulting from the high metabolic activity of L. buchneri in these silages. [source] The effect of harvest date and inoculation on the yield, fermentation characteristics and feeding value of forage pea and field bean silagesGRASS & FORAGE SCIENCE, Issue 3 2001M. D. Fraser Two experiments describe the ensiling potential of whole-crop forage peas (Pisum sativum) and field beans (Vicia faba). In Experiment 1, forage peas (cv. Magnus) and field beans (cv. Mayo) were harvested at 10, 12 and 14 weeks after sowing, and ensiled in 10 kg mini-silos either untreated or treated with an inoculant (Lactobacillus plantarum). In terms of yield and ensiling potential, the optimum growth stage for harvesting forage peas occurred at 12 weeks of growth. In contrast, delaying the harvest of field beans until 14 weeks gave the highest yields of dry matter (DM) and crude protein (CP). Changes in crop maturity had little effect on the chemical composition of the fresh forages, but between-harvest date differences were observed in the DM, ammonia-N, CP, water-soluble carbohydrates (WSC), acid-detergent fibre (ADF), neutral-detergent fibre (NDF), lactic acid and volatile fatty acids (VFA) concentrations and pH of the corresponding silages. Fermentation was improved by applying an inoculant. In Experiment 2, forage peas and field beans were harvested at 14 weeks after sowing and ensiled as round-bale silage, either untreated or treated with an inoculant. The yields of the crops were similar, and the only difference in the chemical composition of the wilted forages was a higher CP concentration in the field beans. However, after the ensiling process was complete, the forage pea silages were found to have significantly higher DM, WSC, starch and butyric acid concentrations compared with the field bean silages, and lower ammonia-N, CP, ADF, acetic acid and lactic acid concentrations. Inoculation was found to increase the lactic acid concentration and reduce the pH and ammonia-N and acetic acid concentrations of the silages. Each of the silages produced in Experiment 2 was offered to six Suffolk crossbred wether lambs, aged 10 months. Voluntary DM intakes were similar on all treatments, despite the apparent digestibility of the forage pea silages being significantly higher than that of the field bean silages. Nitrogen retention was higher for lambs offered forage pea silage. Application of an inoculant was found to have a negative effect on the amount of N retained, indicating the necessity for more detailed investigations into proteolytic activity within these crops during the fermentation process. [source] Cultivation and ensilage of yacon plants (Smallanthus sonchifolius [Poepp.GRASSLAND SCIENCE, Issue 1 2009& Endl.] H. Robinson), the function of yacon silage Abstract Yacon (Smallanthus sonchifolius [Poepp. & Endl.] H. Robinson) plant cultivation has extended rapidly throughout Japan since 1985 because of its utility as a tuberous root crop. In this study, strains with high yields of both above-ground and underground parts were identified to investigate the use of above-ground parts for animal feed silage. In addition, we measured the anti-oxidative activity of the silage as a functional characteristic. Data from 5 years of the cultivation of 17 yacon strains suggest that the most suitable strain for planting in the Aso region of Japan is SY217, which has a high yield of both above-ground parts and tuberous roots. The root tuber parts of yacon are used almost entirely as food; actually, most foliage parts are hardly used, except for some above-ground parts, which are used as tea. The fermentation qualities of the silage using all above-ground parts and harvest residue tuberous roots (harvest residues) are high and their dry matter digestibility, investigated using pigs, is 66.7%. Fresh above-ground parts had very strong anti-oxidative activity as compared with butylated hydroxyanisole and ,-tocopherol. In addition, that activity is retained during ensiling for 4 weeks. These results suggest that the entire yacon plant can be used effectively as a feedstuff resource with the added bonus of anti-oxidant activity. [source] Ensilage of wilted whole crop rice (Oryza sativa L.) using a roll baler for chopped material: Silage quality in long-term storageGRASSLAND SCIENCE, Issue 2 2007Hidenori Kawamoto Abstract We examined the effects of long-term storage on the fermentation quality, chemical composition, and digestibility of wilted whole crop rice silage prepared using a roll baler for chopped material (set chop length, 13 mm) and compared the results with those obtained by using a conventional roll baler. The roll balers were used for ensiling whole crop rice of three types: (i) dough-ripe stage with light wilting (45% dry matter); (ii) dough-ripe stage with heavy wilting (65% dry matter); and (iii) yellow-ripe stage with light wilting (45% dry matter). The apparent dry matter density was higher in the ensiled roll bales composed of chopped whole crop rice (chopped bales) than in those composed of non-chopped whole crop rice (conventional bales) (195,250 kg m,3 vs 156,218 kg m,3, respectively). The formation of volatile fatty acids and ammonia-nitrogen was low in all types of silage. Further, no marked differences in the chemical composition and apparent dry matter digestibility were observed between silage from the two types of bales. However, there were significant differences in their lactic acid and ethanol contents. The lactic acid production in the conventional silage was low (0.08,0.14% fresh matter), whereas that in the chopped silage was high (0.71,0.97% fresh matter). A lower pH value (pH 4.0,4.3) was retained in the chopped silage after 10 months of storage. High ethanol production (1.1,2.5% fresh matter) was observed in the conventional silage, whereas ethanol production decreased to less than 1% in the chopped silage. These results indicate that although the ethanol fermentation is readily enhanced in the ensilage of wilted whole crop rice performed by a conventional baler, the ensilage performed by a baler for chopped material encourages lactic acid fermentation and suppresses ethanol production over a wide moisture range throughout the long-term storage. [source] Fermentation quality and nutritive value of green soybean stover silageGRASSLAND SCIENCE, Issue 1 2007Ken-ichi Horiguchi Abstract The aim of this study was to examine the effect of fermented juice of epiphytic lactic acid bacteria (FJLB) on fermentation quality and nutritive value of green soybean stover silage. Green soybean stover was cut and packed in drum silos without or with 5% (volume/weight) FJLB. Four sheep were fed a basal diet of 40% reed canarygrass hay and 60% commercial concentrate (dry matter basis) and assigned to two treatments: green soybean stover silage preserved without FJLB or with FJLB. Crude protein and neutral detergent fiber contents of green soybean stover silage (dry matter basis) were 16.4% and 45.2% for the silage without FJLB, and 15.8% and 47.1% for the silage with FJLB, respectively. Lactic acid content of the fresh green soybean stover silage was 1.41% for the silage without FJLB and 1.63% for the silage with FJLB. The butyric acid content of the fresh green soybean stover silage with FJLB was substantially lower than that of the silage without FJLB. There was no effect of FJLB added for silage making on the nutrient content, nitrogen retention or ruminal fermentation of the diets. It was estimated that total digestible nutrients content and digestible crude protein content of green soybean stover silage were 64.2% and 11.2% of dry matter, respectively. [source] Use of soiling rice crop silage as feed for growing pigsGRASSLAND SCIENCE, Issue 3 2005Toshiyoshi Takahashi [source] Metabolic and productive response to ruminal protein degradability in early lactation cows fed untreated or xylose-treated soybean meal-based dietsJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 6 2009M. Jahani-Moghadam Summary Effects of different dietary rumen undegradable (RUP) to degradable (RDP) protein ratios on ruminal nutrient degradation, feed intake, blood metabolites and milk production were determined in early lactation cows. Four multiparous (43 ± 5 days in milk) and four primiparous (40 ± 6 days in milk) tie-stall-housed Holstein cows were used in a duplicated 4 × 4 Latin square design with four 21-day periods. Each period had 14-day of adaptation and 7-day of sampling. Diets contained on a dry matter (DM) basis, 23.3% alfalfa hay, 20% corn silage and 56.7% concentrate. Cows were first offered alfalfa hay at 7:00, 15:00 and 23:00 hours, and 30 min after each alfalfa hay delivery were offered a mixture of corn silage and concentrate. Treatments were diets with RUP:RDP ratios of (i) 5.2:11.6 (control), (ii) 6.1:10.6, (iii) 7.1:9.5 and (iv) 8.1:8.5, on a dietary DM% basis. Different RUP:RDP ratios were obtained by partial and total replacement of untreated soybean meal (SBM) with xylose-treated SBM (XSBM). In situ study using three rumen-cannulated non-lactating cows showed that DM and crude protein (CP) of SBM had greater rapidly degradable fractions. The potentially degradable fractions were degraded more slowly in XSBM. Treatment cows produced greater milk, protein, lactose, solids-non-fat and total solids than control cows. Increasing RUP:RDP reduced blood urea linearly. Feed costs dropped at RUP:RDP ratios of 6.1:10.6 and 7.1:9.5, but not at 8.1:8.5, compared with the 5.2:11.6 ratio. Intake of DM and CP, rumen pH, blood glucose, albumin and total protein, faecal and urine pH, changes in body weight and body condition score, and milk lactose and solids-non-fat percentages did not differ among treatments. Results provide evidence that increasing dietary RUP:RDP ratio from 5.2:11.6 to 7.1:9.5 optimizes nitrogen metabolism and milk production and reduces feed costs in early lactation cows. Reduced blood urea suggests reprodutive benefits. [source] Effects of level of feed intake and Fusarium toxin-contaminated wheat on rumen fermentation as well as on blood and milk parameters in cowsJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 3-4 2006K. Seeling Summary The aims of this study were to examine the effects of and possible interactions between dry matter (DM) intake and feeding Fusarium toxin-contaminated wheat on ruminal fermentation, serum chemical parameters and milk yield of dairy cows. Fourteen dairy cows equipped with ruminal and duodenal cannulas were analysed. All animals were fed the same ration, the daily feed amounts being adjusted to current performance. On DM basis, the ration consisted of 60% concentrate including 55% wheat [Fusarium -contaminated wheat (mycotoxin period) or control wheat (control period)] and was completed with 40% maize and grass silage. Each cow was fed the contaminated wheat [deoxynivalenol (DON), 8.21 mg/kg DM and zearalenone (ZON), 0.09 mg/kg DM] and the control wheat (0.25 mg DON/kg DM and 51 ,g ZON/kg DM). As expected, a higher organic matter (OM) intake decreased the amounts of fermented crude nutrients related to the respective intakes. An increased amount of crude protein degraded (p < 0.05) and a lower molar percentage of propionate in the rumen fluid were observed when feeding the Fusarium toxin-contaminated wheat at increased OM intakes in comparison with the control wheat. The activities of serum aspartate aminotransferase (ASAT; p < 0.001), glutamate dehydrogenase (GLDH; p < 0.01) and gamma glutamyl transferase (, -GT; p < 0.01) increased with increasing OM intake and were not related to the mycotoxin contamination of the wheat. [source] Degradation of transgenic Cry1Ab DNA and protein in Bt-176 maize during the ensiling processJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 3-4 2006B. Lutz Summary Maize silage is commonly used as feed for farm animals. The aim of this study was to monitor the time-dependent degradation of non-recombinant chloroplast DNA (exemplified by the rubisco gene) in comparison with the recombinant cry1Ab gene in the course of the ensiling process. In parallel, the Cry1Ab protein content and fragment sizes were determined. Fragments of the rubisco (173, 896, 1197, 1753 and 2521 bp) and of the cry1Ab gene (211, 420, 727 and 1423 bp) were selected to investigate the DNA degradation process. The detection of the Cry1Ab protein was performed using an enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Rubisco gene fragments of 173 bp were still detectable after 61 days, while fragments of 1197 and 2521 bp were detectable up to 30 days and on the first day only respectively. Polymerase chain reaction (PCR) analyses revealed that fragments of the cry1Ab gene with sizes of 211 and 420 bp were detectable up to 61 days, fragments with sizes of 727 and 1423 bp, 30 and 6 days respectively. The ELISA showed a decrease of the Cry1Ab protein in maize silage during the ensiling process. No marked degradation was observed during the first 43 h. Thereafter, a sharp decrease was measured. After 61 days, 23.6 ± 0.9% of the initial Cry1Ab protein was still detectable. Immunoblotting confirmed the results of the ELISA showing a positive signal of approximately 60 kDa size for 8 days of ensiling; no further immunoactive fragments were detectable by immunoblotting. In conclusion, the ensiling process markedly decreases the presence of long functional cry1Ab gene fragments and full size Cry1Ab protein. [source] Selenium supplementation and selenium status of dairy cows fed diets based on grass, grass silage or maize silageJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 3-4 2002M. GIERUS In three separate trial series (TS) the effect of diet composition on selenium (Se) status of dairy cows were investigated. Diets were formulated based mainly on grass (TS1), grass silage (TS2) or maize silage (TS3) with different levels of Se supplementation. Each TS comprised a total of 30 dairy cows and contained one treatment group without Se supplementation (control) and two groups with increasing levels of Se supplementation (levels 1 and 2). Selenium was administered as Na-selenite. The control groups of the different TS showed a very low Se supply of 38,54 ,g Se/kg DM. At level 1 the Se supply was increased to 102,165 ,g Se/kg DM and at level 2 was 294,373 ,g Se/kg DM. After completion of the 6-week trials the average plasma Se concentration of the control cows (without Se supplementation) across all TS was 21.5 ,g/l; this increased significantly following Se supplementation, to 37.7 ,g/l at level 1 and 61.5 ,g/l at level 2. The plasma glutathione peroxidase (GSH-Px) activity of the control cows averaged 67 U/l, rising considerably after supplementation at level 1 to a value of 101 U/l, but showed little further increase at level 2 with a mean value of 120 U/l. By contrast, the average Se content of the milk was unchanged in the control and level 1 groups at 10.5 ,g/kg and 10.9 ,g/kg, respectively, and only increased markedly after supplementation at level 2 to a mean value of 15.1 ,g/kg. The diet based on maize silage, while having a similar Se content as the grass and grass silage-based diets, resulted in a slightly improved Se status, which is due to a higher Se intake from soybean meal. [source] Responses of milk production to the intravenous infusion of amino acids in dairy cows given diets of grass silage and cereal-based supplementsJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 9-10 2001C.-H. Kim Three experiments were carried out to examine responses of milk production to the intravenous infusion of amino acids in dairy cows given diets of grass silage and supplements based on barley, with or without added soyabean meal and ranging in crude protein content from 16 to 19% in dry matter. Particular attention was given to histidine, administered alone or in combination with methionine, lysine and tryptophan. Responses of milk protein secretion to infusion of histidine were seen only when the diet contained a supplement of barley alone. When soyabean meal was included, there were no responses of milk production to infusion of any of the infused amino acids. Calculations suggested that, although histidine remained first-limiting when soya was included in the diet, any response to infusion of histidine was blocked by the rapidly emerging deficiency of another amino acid, probably leucine. The results confirm that, for diets based on grass silage and supplements of cereal only, histidine is first-limiting such that increases of milk protein secretion can be obtained in response to infusion of histidine alone. In assessing the practical significance of this finding, it should be remembered that greater responses in the yield of milk protein can probably be obtained by substituting 1 kg of soyabean meal for 1 kg of cereal, which is likely to be an easier and cheaper option. [source] Testing biotic indicator taxa: the sensitivity of insectivorous mammals and their prey to the intensification of lowland agricultureJOURNAL OF APPLIED ECOLOGY, Issue 1 2008Michael J. O. Pocock Summary 1Changes to agricultural policy aim to extensify agriculture and increase biodiversity. However, it is not known how sensitive many taxa are to intensification. Sensitive taxa could be used as biotic indicators, to assess changes over time and the effectiveness of policy changes. 2We sampled shrews, bats and their prey (beetles, flies and moths) on matched pairs of sites and assessed the response in their abundance to aspects of intensification: increased agrochemical inputs (using abundance on matched organic and conventional cereal fields as a proxy), the switch from hay to silage (using abundance on matched hay and silage fields) and boundary loss (using abundance in the field and near the boundary as a proxy, in cereal and grass separately). We quantified the abundance-derived sensitivity of the taxa in order to assess their use as biotic indicators. 3There was substantial variation in the sensitivity of taxa to the three aspects of intensification. Most estimates (51%) of sensitivity to boundary loss were significant, but only 8% for increased agrochemical inputs and 16% for the switch from hay to silage. Sensitivity to one aspect of intensification was not significantly related to sensitivity to another. 4Insectivorous mammals were relatively insensitive to increased agrochemical inputs and the switch from hay to silage, but strongly sensitive to boundary loss. 5Taxa with significant sensitivity to increased agrochemical inputs included some Carabidae and Diptera. We found fewer significant differences in abundance between organic and conventional farms than previous workers, probably because we controlled for boundary characteristics, suggesting that the quality of field boundaries is important in influencing biodiversity differences between organic and conventional farms. 6The switch from hay to silage had a positive effect on some Coleoptera and Diptera but a substantial negative effect on Hepialidae (Lepidoptera). 7Synthesis and applications. The recorded sensitivity of taxa to changes in agricultural practices is highly variable. Therefore, the selection of biotic indicator taxa of agricultural intensification is not straightforward. If surveys of biotic indicator taxa are used to assess the effectiveness of changes in agricultural practice, empirical evidence should be used to select suitable taxa. [source] The use of a quantitative real-time polymerase chain reaction assay for identification and enumeration of Lactobacillus buchneri in silageJOURNAL OF APPLIED MICROBIOLOGY, Issue 3 2008R.J. Schmidt Abstract Aims:, To detect and quantify Lactobacillus buchneri in plant samples with the aid of polymerase chain reaction (PCR) methods. Methods and Results:, DNA from silage samples spiked with different amounts of L. buchneri cells was isolated using a lysozyme/sodium dodecyl sulfate lysis and phenol/chloroform extraction method. The DNA served as a template for PCR amplification with primers specific for the bacterium. The primers were developed by comparison of 16S rDNA sequences from different lactic acid bacteria (LAB) and testing for specificity with 11 different strains of LAB. As few as 100 L. buchneri colony-forming units per gram of silage could be detected. Additionally, the technique was successfully applied to quantify the population of L. buchneri in two cultivars of corn with or without inoculation. Conclusions:, The PCR assay provided a specific and rapid tool for identifying and enumerating L. buchneri in silage samples. Significance and Impact of the Study:, The use of microbial inoculants for silage production is a safe and environment friendly practice, but the full potential of such additives can only be achieved with a better understanding of the fate and activity of the microbes involved. The current study describes a methodology to detect and enumerate L. buchneri, a micro-organism used as an inoculant. [source] Bacteriological and chemical changes occurring in Bunker-stored silage covered with biodegradable coatingJOURNAL OF APPLIED MICROBIOLOGY, Issue 2 2007P. Denoncourt Abstract Aims:, To evaluate the efficacy of a biodegradable silage coating for the ability to protect timothy (Phleum pratensa) type silage against spoilage and its quality under natural conditions. Methods and Results:, Triplicate mini-silos of silage were prepared for three treatments (1: uncoated; 2: coated with biodegradable coating and 3: sealed with plastic), two types of storage (unprotected or protected from rain) and 10 sampling times (0, 7, 14, 21, 28, 35, 42, 56, 63 and 70 days postensiling). Triplicate mini-silos were opened at each sampling time for microbiological (total aerobic bacteria, lactic acid bacteria, moulds and yeasts) and biochemical analyses [pH, dry matter (DM), water-soluble sugars (WSC), lactic (LA), acetic, propionic and butyric acids content]. The study showed that at day 70, counts of moulds and yeasts in silages protected against rain and coated with biodegradable coating were 5·98 log CFU g,1 when compared with 5·92 and 3·62 log CFU g,1 in samples from plastic-sealed silage and uncoated silage, respectively. The pH was low and stable pH (4·34) when compared with uncoated (7·17) and plastic sealed (8·34) silages (P 0·05). A DM, WSC and LA content of 421·7, 13·4 and 20·9 g kg,1 was, respectively, observed. For silage stored outdoors, a level of moulds and yeasts of 3·77 log CFU g,1 of silage was also observed in silages coated with biodegradable coating after 28 days of storage. A stable pH showing a mean value of 4 was also observed. The pH, DM, WSC and LA content were, respectively, 4·18, 341·1, 13·34 and 31·8 g kg,1 in these samples. After 70 days of storage, the level of moulds and yeasts on silage sealed with biodegradable coating was 7·73 log CFU g,1. A DM, WSC and LA content of 291·9, 5·56 and 10·0 g kg,1 was, respectively, observed. Conclusions:, When compared with uncoated silage, the application of biodegradable coating can preserve the quality of silage for up to a month when exposed to rain and up to 70 days when protected from rain. Significance and Impact of the Study:, Results emphasize the possibility of the use of a biodegradable coating as an alternative to plastic film for sealing horizontal bunker silos. [source] Combination of a urease inhibitor and a plant essential oil to control coliform bacteria, odour production and ammonia loss from cattle wasteJOURNAL OF APPLIED MICROBIOLOGY, Issue 2 2007V.H. Varel Abstract Aim:, To evaluate urea hydrolysis, volatile fatty acid (VFA) production (odour) and coliforms in cattle waste slurries after a urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) and a plant oil component (thymol) were added. Methods and Results:, Faeces from cattle fed a diet of 70% corn silage and 30% alfalfa haylage, urine and distilled water in the ratio 50 : 35 : 15 were blended at high speed for 1 min. Triplicate aliquots of 750 ml were amended with NBPT plus or minus thymol and reblended for 1 min, and were poured into 1·6 l wide-mouth jars covered 90% with a lid. After 56 days, thymol (2000 mg kg,1 waste) in combination with NBPT (80 mg kg,1 waste) retained 5·2 g of an initial 9·2 g of urea in cattle waste slurries, compared with less than 1 g of urea retained when NBPT was the only additive (P < 0·05). Another experiment using excreta from cattle fed 76·25% high moisture corn, 19·25% corn silage and a 4·5% supplement, blended at a low speed, gave a similar response with urea hydrolysis; and the two treatments, thymol alone and thymol in combination with NBPT, reduced VFA production (P < 0·01) and eliminated all coliform bacteria by day 1. A third experiment indicated coliforms disappeared in the no addition treatment after 8 days; however, they were viable at 6·6 × 104 CFU g,1 waste beyond 35 days in the NBPT treatment. Conclusions:, Thymol supplements the effect of NBPT by increasing the inhibitory period for hydrolysis of urea in cattle waste slurries and nitrogen retention in the waste. Significance and Impact of the Study:, Thymol and NBPT offer the potential to reduce odour and pathogens in cattle manure, and increase the fertilizer value. [source]
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