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Signal Attenuation (signal + attenuation)
Selected AbstractsInterleukin-6-induced proliferation of pre-B cells mediated by receptor complexes lacking the SHP2/SOCS3 recruitment sites revisitedFEBS JOURNAL, Issue 24 2001Kerstin Friederichs Interleukin-6 (IL-6) induces B-cell proliferation by binding to receptor complexes composed of a specific ,-receptor (gp80; CD126) and the signal transducing receptor subunit gp130 (CD130). Immediately after receptor complex activation, signal transducers and activators of transcription (STATs) 1 and 3 and the Src-homology domain-containing protein tyrosine phosphatase 2 (SHP2) are recruited to gp130 and subsequently tyrosine phosphorylated. The activated dimerized STATs translocate to the nucleus and bind to enhancer elements of IL-6-inducible genes. SHP2 acts as an adapter and links the Jak/STAT pathway to the Ras/Raf/MAPK cascade but it is also involved in signal attenuation. Whereas STAT3 activation appears to be crucial for all biological activities of IL-6, the requirement of SHP2-activation depends on the individual biological response analyzed. The requirement of SHP2 activation for the pre-B cell (Ba/F3) proliferation has been reported previously [Fukada, T., Hibi, M., Yamanaka, Y., Takahashi-Tezuka, M., Fujitani, Y., Yamaguchi, T., Nakajima, K. & Hirano, T. (1996) Immunity5, 449,460]. In contrast, we have recently demonstrated that the presence of a single STAT-recruitment site within gp130 is sufficient for IL-6- induced proliferation of Ba/F3 cells [Schmitz, J., Dahmen, H., Grimm, C., Gendo, C., Müller-Newen, G., Heinrich, P.C. & Schaper, F. (2000) J. Immunol.164, 848,854]. To unravel this discrepancy we analyzed the IL-6-induced dose-dependent proliferation of Ba/F3 cells mediated by receptor complexes lacking SHP2/SOCS3 recruitment sites. Surprisingly, pre-B cells, after stimulation with low amounts of IL-6, proliferate much more efficiently in the absence of the activated SHP2 than in the presence of the tyrosine phosphatase. Therefore, SHP2 activation appears to be relevant for IL-6-induced proliferation only after stimulation with very large amounts of IL-6. [source] Magnetic Resonance Sounding: New Method for Ground Water AssessmentGROUND WATER, Issue 2 2004M. Lubczynski The advantage of magnetic resonance sounding (MRS) as compared to other classical geophysical methods is in its water selective approach and reduced ambiguity in determination of subsurface free water content and hydraulic properties of the media due to the nuclear magnetic resonance (NMR) principle applied. Two case examples are used to explain how hydrogeological parameters are obtained from an MRS survey. The first case example in Delft (the Netherlands) is a multiaquifer system characterized by large signal to noise ratio (S/N = 73), with a 24 m thick, shallow sand aquifer, confined by a 15 m thick clay layer. For the shallow aquifer, a very good match between MRS and borehole data was obtained with regard to effective porosity nc,28% and specific drainage Sd,20%. The MRS interpretation at the level deeper than 39 m was disturbed by signal attenuation in the low resistivity (,10 ,m) media. The second case of Serowe (Botswana) shows a fractured sandstone aquifer where hydrogeological parameters are well defined at depth >74 m below ground surface despite quite a low S/N = 0.9 ratio, thanks to the negligible signal attenuation in the resistive environment. Finally, capabilities and limitations of the MRS technology are reviewed and discussed. MRS can contribute to subsurface hydrostratigraphy description, hydrogeological system parameterization, and improvement of well siting. The main limitations are survey dependence upon the value of the S/N ratio, signal attenuation in electrically conductive environments, nonuniformity of magnetic field, and some instrumental limitations. At locations sufficiently resistive to disregard the signal attenuation problems, the MRS S/N ratio determines how successfully MRS data can be acquired. Both signal and noise vary spatially; therefore, world scale maps providing guidelines on spatial variability of signal and noise are presented and their importance with respect to the MRS survey results is discussed. The noise varies also temporally; therefore, its diurnal and seasonal variability impact upon the MRS survey is covered as well. [source] Addressing a systematic vibration artifact in diffusion-weighted MRIHUMAN BRAIN MAPPING, Issue 2 2010Daniel Gallichan Abstract We have identified and studied a pronounced artifact in diffusion-weighted MRI on a clinical system. The artifact results from vibrations of the patient table due to low-frequency mechanical resonances of the system which are stimulated by the low-frequency gradient switching associated with the diffusion-weighting. The artifact manifests as localized signal-loss in images acquired with partial Fourier coverage when there is a strong component of the diffusion-gradient vector in the left,right direction. This signal loss is caused by local phase ramps in the image domain which shift the apparent k-space center for a particular voxel outside the covered region. The local signal loss masquerades as signal attenuation due to diffusion, severely disrupting the quantitative measures associated with diffusion-tensor imaging (DTI). We suggest a way to improve the interpretation of affected DTI data by including a co-regressor which accounts for the empirical response of regions affected by the artifact. We also demonstrate that the artifact may be avoided by acquiring full k-space data, and that subsequent increases in TE can be avoided by employing parallel acceleration. Hum Brain Mapp, 2010. © 2009 Wiley-Liss, Inc. [source] Seasonal and diurnal variations of total attenuation measured with the ITALSAT satellite at Spino d'Adda at 18.7, 39.6 and 49.5 GHzINTERNATIONAL JOURNAL OF SATELLITE COMMUNICATIONS AND NETWORKING, Issue 4 2004Carlo Riva Abstract The ITALSAT satellite experiment started in January 1991 and ended in January 2001, permitting an extensive program of propagation measurements at 18.7, 39.6 and 49.5 GHz. In these frequency bands, up- and down-links experience severe signal attenuation due to meteorological effects such as those due to gas (oxygen and water vapour), clouds, turbulence and, especially, rain. The propagation measurement campaigns aim mainly at assessing and at modelling the appropriate fade margin compensating propagation attenuation in the design of satellite communication systems. This margin depends significantly on the season and on the time of the day, due to variations of meteorological conditions. This paper reports the results obtained from copolar signal measurements carried out at the Earth station of Spino d'Adda, near Milano (North Italy), at the three frequencies during 7 years (from 1994 to 2000). The measured cumulative distribution functions of total attenuation are compared to ITU-R models' prediction. Moreover the statistics conditioned to single months of the year, seasons and 4 h contiguous periods of the day are also shown. These statistics can be useful for telecommunication systems whose service quality and design must be matched to the season of the year or the time of the day. Copyright © 2004 John Wiley & Sons, Ltd. [source] Effects of cord motion on diffusion imaging of the spinal cordMAGNETIC RESONANCE IN MEDICINE, Issue 2 2006Hardave S. Kharbanda Abstract Measurement of diffusion and its dependence on direction has become an important tool for clinical and research studies of the brain. Diffusion imaging of the spinal cord may likewise prove useful as an indicator of tissue damage and axonal integrity; however, it is more challenging to perform diffusion imaging in the cord than in the brain. Here we report a study of the effects of motion on single-shot fast spin echo (FSE) diffusion tensor imaging (DTI) of the spinal cord. Diffusion imaging was performed at four different times in the cardiac cycle both without and with velocity compensation of the diffusion gradients. Uncompensated diffusion images demonstrated substantial signal loss artifacts in the cord that were strongly dependent on the delay after the pulse-oximeter trigger. Quantitative diffusion analysis was also strongly affected by this motion artifact. The use of flow-compensated gradients helped to restore normal signal in the cord, especially at particular trigger delays. Theoretical arguments suggest that improved spatial resolution may help eliminate this signal loss. Even with higher spatial resolution, motion-related signal attenuation may still occur in diffusion imaging of pathologies that alter the motion of the cord. However, this same cord motion may contain diagnostically valuable information when probed using appropriate diffusion imaging approaches. Magn Reson Med, 2006. © 2006 Wiley-Liss, Inc. [source] Segmentation and Estimation for SNP Microarrays: A Bayesian Multiple Change-Point ApproachBIOMETRICS, Issue 3 2010Yu Chuan Tai Summary High-density single-nucleotide polymorphism (SNP) microarrays provide a useful tool for the detection of copy number variants (CNVs). The analysis of such large amounts of data is complicated, especially with regard to determining where copy numbers change and their corresponding values. In this article, we propose a Bayesian multiple change-point model (BMCP) for segmentation and estimation of SNP microarray data. Segmentation concerns separating a chromosome into regions of equal copy number differences between the sample of interest and some reference, and involves the detection of locations of copy number difference changes. Estimation concerns determining true copy number for each segment. Our approach not only gives posterior estimates for the parameters of interest, namely locations for copy number difference changes and true copy number estimates, but also useful confidence measures. In addition, our algorithm can segment multiple samples simultaneously, and infer both common and rare CNVs across individuals. Finally, for studies of CNVs in tumors, we incorporate an adjustment factor for signal attenuation due to tumor heterogeneity or normal contamination that can improve copy number estimates. [source] In situ near infrared spectroscopy for analyte-specific monitoring of glucose and ammonium in streptomyces coelicolor fermentationsBIOTECHNOLOGY PROGRESS, Issue 1 2010Nanna Petersen Abstract There are many challenges associated with in situ collection of near infrared (NIR) spectra in a fermentation broth, particularly for highly aerated and agitated fermentations with filamentous organisms. In this study, antibiotic fermentation by the filamentous bacterium Streptomyces coelicolor was used as a model process. Partial least squares (PLS) regression models were calibrated for glucose and ammonium based on NIR spectra collected in situ. To ensure that the models were calibrated based on analyte-specific information, semisynthetic samples were used for model calibration in addition to data from standard batches. Thereby, part of the inherent correlation between the analytes could be eliminated. The set of semisynthetic samples were generated from fermentation broth from five separate fermentations to which different amounts of glucose, ammonium, and biomass were added. This method has previously been used off line but never before in situ. The use of semisynthetic samples along with validation on an independent batch provided a critical and realistic evaluation of analyte-specific models based on in situ NIR spectroscopy. The prediction of glucose was highly satisfactory resulting in a RMSEP of 1.1 g/L. The prediction of ammonium based on NIR spectra collected in situ was not satisfactory. A comparison with models calibrated based on NIR spectra collected off line suggested that this is caused by signal attenuation in the optical fibers in the region above 2,000 nm; a region which contains important absorption bands for ammonium. For improved predictions of ammonium in situ, it is suggested to focus efforts on enhancing the signal in that particular region. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010 [source] Optimized balanced steady-state free precession magnetization transfer imagingMAGNETIC RESONANCE IN MEDICINE, Issue 3 2007O. Bieri Abstract Balanced steady-state free precession (bSSFP) suffers from a considerable signal loss in tissues. This apparent signal reduction originates from magnetization transfer (MT) and may be reduced by an increase in repetition time or by a reduction in flip angle. In this work, MT effects in bSSFP are modulated by a modification of the bSSFP sequence scheme. Strong signal attenuations are achieved with short radio frequency (RF) pulses in combination with short repetition times, whereas near full, i.e., MT-free, bSSFP signal is obtained by a considerable prolongation of the RF pulse duration. Similar to standard methods, the MT ratio (MTR) in bSSFP depends on several sequence parameters. Optimized bSSFP protocol settings are derived that can be applied to various tissues yielding maximal sensitivity to MT while minimizing contribution from other impurities, such as off-resonances. Evaluation of MT in human brain using such optimized bSSFP protocols shows high correlation with MTR values from commonly used gradient echo (GRE) sequences. In summary, a novel method to generate MTR maps using bSSFP image acquisitions is presented and factors that optimize and influence this contrast are discussed. Magn Reson Med 58:511,518, 2007. © 2007 Wiley-Liss, Inc. [source] | |||||||||||||