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Situ Experiments (situ + experiment)
Selected AbstractsUnravelling the microbial role in ooid formation , results of an in situ experiment in modern freshwater Lake Geneva in SwitzerlandGEOBIOLOGY, Issue 4 2008K. PLEE ABSTRACT The microbial role in the formation of the cortex of low-Mg calcite freshwater ooids in western part of Lake Geneva in Switzerland has been suggested previously, but not demonstrated conclusively. Early work mostly concentrated in hypersaline milieus, and hence little is known about their genesis in freshwater environments. We designed an in situ experiment to mimic the natural process of low-Mg calcite precipitation. A special device was placed in the ooid-rich bank of the lake. It contained frosted glass (SiO2) slides, while quartz (SiO2) is the most abundant mineral composition of ooid nuclei that acted as artificial substrates to favour microbial colonization. Microscopic inspection of the slides revealed a clear seasonal pattern of carbonate precipitates, which were always closely associated with biofilms that developed on the surface of the frosted slides containing extracellular polymeric substance, coccoid and filamentous cyanobacteria, diatoms and heterotrophic bacteria. Carbonate precipitation peaks during early spring and late summer, and low-Mg calcite crystals mostly occur in close association with filamentous and coccoid cyanobacteria (e.g. Tolypothrix, Oscillatoria and Synechococcus, Anacystis, respectively). Further scanning electron microscope inspection of the samples revealed low-Mg calcite with crystal forms varying from anhedral to euhedral rhombohedra, depending on the seasons. Liquid cultures corroborate the in situ observations and demonstrate that under the same physicochemical conditions the absence of biofilms prevents the precipitation of low-Mg calcite crystals. These results illustrate that biofilms play a substantial role in low-Mg calcite ooid cortex formation. It further demonstrates the involvement of microbes in the early stages of ooid development. Combined with ongoing microbial cultures under laboratory-controlled conditions, the outcome of our investigation favoured the hypothesis of external microbial precipitation of low-Mg calcite as the main mechanism involved in the early stage of ooid formation in freshwater Lake Geneva. [source] Cardiac disorders in farmed adult brown trout, Salmo trutta L.JOURNAL OF FISH DISEASES, Issue 4 2000C Mercier During summer in Brittany, France, sea farmed brown trout, Salmo trutta L., regularly experience a high mortality rate which is associated, at least in part, with cardiac disorders (aneurysms and infarcts). The present study is preliminary to a more extensive research programme, the objective of which is to determine to what extent the physiological performance of the cardiovascular system of brown trout is affected by the environmental conditions the fish experience in farm cages. We conducted a 2-week in situ experiment during which the heart rate of eight sea water acclimatized individuals was telemetered using acoustic tags. During the experimental period, water temperature ranged from 16.0 to 17.6 °C. Water oxygen saturation was above 80% at all times and salinity was very high (35.5,) but stable. Although they were unfed and not active, seven of out the eight tagged animals displayed near maximum heart beat frequencies, which ranged between 83 and 98 beats per minute (bpm). On the other hand, the eighth animal exhibited medium-range heart rates (50,70 bpm). Using phase delay maps, we established that the maximum heart rate of brown trout at 17 °C was in the range of 96,100 bpm. This result suggests that in our experimental conditions, the heart rate of most of our inactive fish was between 85 and 100% of maximum myocardial performance. We hypothesize that the cardiac failures observed in brown trout during summer are most likely a result of strenuous workloads imposed on the cardiovascular system by a combination of elevated temperature, high salinity and possibly season-related decreased hypo-osmoregulatory abilities. [source] Chemical composition and ruminal degradability of lucerne (Medicago sativa) productsJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 15 2001Arif F Mustafa Abstract A study was conducted to determine the chemical composition and in situ nutrient ruminal degradability of three lucerne products. These were dehydrated pellets, sun-cured pellets and cubes. Results of the chemical analysis showed that sun-cured pellets had the highest (P,<,0.05) neutral and acid detergent fibre as well as total carbohydrate levels, followed by cubes and dehydrated pellets respectively. Crude protein (CP) content was highest (P,<,0.05) for dehydrated pellets (204.3,g,kg,1), intermediate for sun-cured pellets (160.0,g,kg,1) and lowest for cubes (153.2,g,kg,1). Intermediately degradable CP (buffer-insoluble CP minus neutral detergent-insoluble CP) was the main protein fraction in the three products and was higher (P,<,0.05) in cubes than in dehydrated and sun-cured pellets. Estimated net energy of lactation was highest (P,<,0.05) for dehydrated pellets (5.9,MJ,kg,1), intermediate for cubes (5.23,MJ,kg,1) and lowest (P,<,0.05) for sun-cured pellets (5.15,MJ,kg,1). Results of the in situ experiment indicated that dehydrated pellets had higher (P,<,0.05) ruminal protein degradability than sun-cured pellets and cubes. The estimated ruminal escape protein values for dehydrated pellets, sun-cured pellets and cubes were 361, 420 and 498,g,kg,1 CP respectively. It was concluded that differences in chemical composition and ruminal degradability among the three lucerne products were mainly due to differences in stage of maturity. It was also concluded that the dehydration process failed to increase the ruminal escape protein value of dehydrated pellets relative to sun-cured pellets and cubes. © 2001 Society of Chemical Industry [source] Factors influencing bacterial dynamics along a transect from supraglacial runoff to proglacial lakes of a high Arctic glacieriFEMS MICROBIOLOGY ECOLOGY, Issue 2 2007Birgit Mindl Abstract Bacterial production in glacial runoff and aquatic habitats along a c. 500 m transect from the ablation area of a Svalbard glacier (Midre Lovénbreen, 79°N, 12°E) down to a series of proglacial lakes in its forefield were assessed. In addition, a series of in situ experiments were conducted to test how different nutrient sources (glacial flour and dissolved organic matter derived from goose faeces) and temperature affect bacterial abundance and production in these ecosystems. Bacterial abundance and production increased significantly along this transect and reached a maximum in the proglacial lakes. Bacterial diversity profiles as assessed by denaturing gradient gel electrophoresis indicated that communities in glacial runoff were different from those in proglacial lakes. Heterotrophic bacterial production was mainly controlled by temperature and phosphorus limitation. Addition of both glacial flour and dissolved organic matter derived from goose faeces stimulated bacterial production in those lakes. The results suggest that glacial runoff sustains an active bacterial community which is further stimulated in proglacial lakes by higher temperatures and nutrient inputs from bird faeces. Thus, as in maritime temperate and Antarctic settings, bacterial communities developing in the recently deglaciated terrain of Svalbard receive important inputs of nutrients via faunal transfers from adjacent ecosystems. [source] Effects of temperature and sediment properties on benthic CO2 production in an oligotrophic boreal lakeFRESHWATER BIOLOGY, Issue 8 2010IRINA BERGSTRÖM Summary 1. Temperature and many other physical and chemical factors affecting CO2 production in lake sediments vary significantly both seasonally and spatially. The effects of temperature and sediment properties on benthic CO2 production were studied in in situ and in vitro experiments in the boreal oligotrophic Lake Pääjärvi, southern Finland. 2. In in situ experiments, temperature of the water overlying the shallow littoral sediment varied seasonally between 0.5 and 15.7 °C, but in deep water (,20 m) the range was only 1.1,6.6 °C. The same exponential model (r2 = 0.70) described the temperature dependence at 1.2, 10 and 20 m depths. At 2.5 and 5 m depths, however, the slopes of the two regression models (r2 = 0.94) were identical but the intercept values were different. Sediment properties (wet, dry, mineral and organic mass) varied seasonally and with depth, but they did not explain a significantly larger proportion of variation in the CO2 output rate than temperature. 3. In in vitro experiments, there was a clear and uniform exponential dependence of CO2 production on temperature, with a 2.7-fold increase per 10 °C temperature rise. The temperature response (slope of regression) was always the same, but the basic value of CO2 production (intercept) varied, indicating that other factors also contributed to the benthic CO2 output rate. 4. The annual CO2 production of the sediment in Lake Pääjärvi averaged 62 g CO2 m,2, the shallow littoral at 0,3 m depth releasing 114 g CO2 m,2 and deep profundal (>15 m) 30 g CO2 m,2. On the whole lake basis, the shallow littoral at 0,3 m depth accounted for 53% and the sediment area in contact with the summer epilimnion (down to a depth c. 10 m) 75% of the estimated total annual CO2 output of the lake sediment, respectively. Of the annual production, 83% was released during the spring and summer. 5. Using the temperature-CO2 production equations and climate change scenarios we estimated that climatic warming might increase littoral benthic CO2 production in summer by nearly 30% from the period 1961,90 to the period 2071,2100. [source] A finite element algorithm for parameter identification of material models for fluid saturated porous mediaINTERNATIONAL JOURNAL FOR NUMERICAL AND ANALYTICAL METHODS IN GEOMECHANICS, Issue 5 2001R. Mahnken Abstract In this contribution an algorithm for parameter identification of geometrically linear Terzaghi,Biot-type fluid-saturated porous media is proposed, in which non-uniform distributions of the state variables such as stresses, strains and fluid pore pressure are taken into account. To this end a least-squares functional consisting of experimental data and simulated data is minimized, whereby the latter are obtained with the finite element method. This strategy allows parameter identification based on in situ experiments. In order to improve the efficiency of the minimization process, a gradient-based optimization algorithm is applied, and therefore the corresponding sensitivity analysis for the coupled two-phase problem is described in a systematic manner. For illustrative purpose, the performance of the algorithm is demonstrated for a slope stability problem, in which a quadratic Drucker,Prager plasticity model for the solid and a linear Darcy law for the fluid are combined. Copyright © 2001 John Wiley & Sons, Ltd. [source] Leaf litter breakdown in Patagonian streams: native versus exotic trees and the effect of invertebrate sizeAQUATIC CONSERVATION: MARINE AND FRESHWATER ECOSYSTEMS, Issue 2 2002R. J. Albariño Abstract 1.Nothofagus native forest in the Southern Andes is being progressively substituted by forestation with rapid growth of the exotic trees, mainly species of Pinaceae. The effect on stream processing dynamics is explored through in situ experiments. 2.The effects of leaf litter quality and macroinvertebrate size on in situ litter breakdown were analysed. Experiments were run in litter bags which allowed access of macroinvertebrate fauna in streams running through a dense forest of the deciduous Nothofagus pumilio. 3.In Experiment 1, the decay rates of N. pumilio leaves and Pinus ponderosa needles were measured during an autumn-winter period. N. pumilio decayed twice as fast as P. ponderosa (P<0.01). Shredders fed only on N. pumilio leaves. The total abundance of macroinvertebrates colonizing both treatments was similar; however, the biomass was higher in the N. pumilio treatment. Large shredders were only found colonizing N. pumilio leaves. Since no decay due to shredders was observed in P. ponderosa, the presence of macroinvertebrates in these litter bags was related to refuge and feeding on FPOM-biofilm resources. 4.In Experiment 2, N. pumilio leaf litter was exposed in order to allow (open bags) or restrict (closed bags) access of invertebrates. The invertebrate assemblage in open bags showed the similar pattern observed for N. pumilio in the first experiment. Gathering-collectors were generally smaller and dominant in number while shredder biomass was higher in open bags as a result of high individual biomass. N. pumilio decayed faster when the whole size spectrum of macroinvertebrates colonized the bags (P<0.01). However, feeding signs of small shredders were observed in closed bags, therefore their role on leaf litter breakdown in Andean streams should not be neglected. 5.The combined results of both experiments lead to the conclusion that the whole litter processing mechanism would be affected as a consequence of the substitution of native forest by exotic pine forestation. Copyright © 2002 John Wiley & Sons, Ltd. [source] NOx emissions of an opposed wall-fired pulverized coal utility boilerASIA-PACIFIC JOURNAL OF CHEMICAL ENGINEERING, Issue 3 2010Xiaotao Gao Abstract The present paper was addressed to the effects of operational variables and coal properties on NOx emission level of a 600-MW opposed wall-fired pulverized coal utility boiler. The in situ experiments were performed to study the effects of operational variables on boiler performance by changing individual variables while the other variables were held nearly constant on the basis of the nominal load operation. It was found that the oxygen level was a significant parameter to affect the NOx emission. The impacts of coal properties on NOx emission indicated that NOx emission level decreased with the increase of the ratio of the nitrogen content to low heating value because the volatile matter content in coal significantly influenced the in-flame NOx formation. Coal volatile content was the dominant parameter to affect fuel NOx formation through affecting the reducing condition in the inner near-burner zone when the low NOx burners were applied in the boiler. Copyright © 2009 Curtin University of Technology and John Wiley & Sons, Ltd. [source] Effect of phytate-removal and deamidation of soybean proteins on calcium absorption in the in situ ratsBIOFACTORS, Issue 1-4 2004Hitomi Kumagai Abstract Soybean proteins were deamidated by cation-exchange resins after phytate, the inhibitor for calcium absorption from the small intestine, was removed in order to provide the enhancement function of calcium absorption to soybean proteins. About 92% of the phosphorus was removed from the soybean proteins by anion-exchange-resin treatment, indicating that most of the phytate was removed. About 70% of the acid amide was deamidated by cation-exchange-resin treatment, and phytate-removed and deamidated soybean proteins (PrDS) having high calcium binding properties were obtained. PrDS were hydrolyzed by digestive enzymes and their calcium-binding properties and the enhancement function of the calcium absorption from the small intestine of rats were examined. As a result, PrDS retained their high calcium binding properties even after hydrolysis by digestive enzymes. in situ experiments showed that PrDS and their hydrolysates enhanced the calcium absorption from the intestine. [source] Genetic structure of the endangered perennial plant Eryngium alpinum (Apiaceae) in an alpine valleyBIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, Issue 4 2008MYRIAM GAUDEUL We investigated the genetic structure of Eryngium alpinum (Apiaceae) in an Alpine valley where the plant occurs in patches of various sizes. In a conservation perspective, our goal was to determine whether the valley consists of one or several genetic units. Habitat fragmentation and previous observations of restricted pollen/seed dispersal suggested pronounced genetic structure, but gene dispersal often follows a leptokurtic distribution, which may lead to weak genetic structure. We used nine microsatellite loci and two nested sampling designs (50 × 50 m grid throughout the valley and 2 × 2 m grid in two 50 × 10 m quadrats). Within the overall valley, F -statistics and Bayesian approaches indicated high genetic homogeneity. This result might be explained by: (1) underestimation of long-distance pollen/seed dispersal by in situ experiments and (2) too recent fragmentation events to build up genetic structure. Spatial autocorrelation revealed isolation by distance on the overall valley but this pattern was much more pronounced in the 50 × 10 m quadrats sampled with a 2-m mesh. This was probably associated with limited primary seed dispersal, leading to the spatial clustering of half-sibs around maternal plants. We emphasize the interest of nested sampling designs and of combining several statistical tools. © 2008 The Linnean Society of London, Biological Journal of the Linnean Society, 2008, 93, 667,677. [source] | ||||||||||||||||