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Situ Data (situ + data)
Selected AbstractsInfluence of calcium on the proteolytic degradation of the calmodulin-like skin protein (calmodulin-like protein 5) in psoriatic epidermisEXPERIMENTAL DERMATOLOGY, Issue 6 2006Bruno Méhul Abstract:, The calmodulin-like skin protein (CLSP) or so-called calmodulin-like protein 5, a recently discovered skin-specific calcium-binding protein, is closely related to keratinocyte differentiation. The 16-kDa protein is proteolytically degraded in the upper layers of the stratum corneum (SC) of healthy skin. With the use of specific new monoclonal antibodies to CLSP, we were able to demonstrate that the abnormal elevated levels of CLSP, characteristic of psoriatic epidermis, were probably not due to an overexpression of the protein, but most likely the result of its non-degradation. Further in vitro experiments using recombinant CLSP and in situ data clearly showed that calcium protected and chelator accelerated CLSP degradation. These data indicate that CLSP degradation in the SC of psoriatic skin might be hindered by the abnormally elevated calcium concentration. No degradation of CLSP in psoriatic epidermis keeping its ability to bind protein as transglutaminase 3 may have a physiological role in skin diseases such as psoriasis. [source] Cholesterol homeostasis markers are localized to mouse hippocampal pyramidal and granule layersHIPPOCAMPUS, Issue 8 2010Chris M. Valdez Abstract Changes in brain cholesterol homeostasis are associated with multiple diseases, such as Alzheimer's and Huntington's; however, controversy persists as to whether adult neurons produce their own cholesterol, or if it is outsourced to astrocytes. To address this issue, we analyzed 25 genes most immediately involved in cholesterol homeostasis from in situ data provided by the Allen Brain Mouse Atlas. We compared the relative mRNA expression in the pyramidal and granule layers, populated with neurons, with the rest of the hippocampus which is populated with neuronal processes and glia. Comparing the expression of the individual genes to markers for neurons and astrocytes, we found that cholesterol homeostasis genes are preferentially targeted to neuronal layers. Therefore, changes in gene expression levels might affect neuronal populations directly. © 2010 Wiley-Liss, Inc. [source] Development of a New Comprehensive Ocean Atlas for Indian Ocean utilizing ARGO DataINTERNATIONAL JOURNAL OF CLIMATOLOGY, Issue 2 2010B. Prasad Kumar Abstract The World Ocean Atlas (WOA), also termed ,Levitus Climatology', is a global ocean climatology containing monthly, seasonal and annual means of temperature (T) and salinity (S) fields at standard ocean depths. The monthly climatology for T and S is available for standard depths up to 1000 m. The database used in the preparation of this climatology (WOA) are historical records of Conductivity, Temperature and Depth (CTD) casts and other available marine observations collected in the past. The methodology used in preparation of this WOA is objective analysis which is essentially non-synoptic and widely scattered in the space domain. We understand that ARGO data has so far not been blended with WOA, nor has its impact for improving WOA climatology been attempted. Presently, with the wealth of marine data from ARGO profilers in the Indian Ocean, we propose a new approach to reconstruct T and S fields optimally utilizing the ARGO data. Here we develop a new model using Delaunay Tessellation with QHull algorithm delivering three-dimensional T and S fields from a non-uniform scattered database up to a depth of 1000 m. For gaps in a data-sparse region, we use all available quality-checked Ocean Station Data (OSD) and Profiling Float Data (PFL) information on T and S, in addition to the existing ARGO data. The initiative here was to replace WOA data points with realistic information from ARGO and in situ data, thereby producing a new climatology atlas. We demonstrate the robustness of our approach, and the final climatology on T and S is better compared with the existing state-of-the-art WOA. The advantage of the proposed methodology is the scope of improving the ocean atlas with the addition of more ARGO data in the near future. The clustered approach in modelling enables ocean parameter retrieval in geometrically disconnected regions with an option for hot restart. We believe that the new climatology will benefit the research community immensely. Copyright © 2009 Royal Meteorological Society [source] Validation of ECMWF (re)analysis surface climate data, 1979,1998, for Greenland and implications for mass balance modelling of the ice sheetINTERNATIONAL JOURNAL OF CLIMATOLOGY, Issue 2 2001Edward Hanna Abstract Climate (re)analysis products are potentially valuable tools, when properly verified, for helping to constrain the surface mass balance of the Greenland Ice Sheet (GIS). Monthly surface fields from European Centre for Medium-Range Weather Forecasts (ECMWF) operational- and re-analyses spanning 1979,1998 were validated using in situ data (surface air pressure and temperature, precipitation, cloud cover, short-/all-wave radiation, and wind speed/direction). These validation data are from coastal or near-coastal Danish Meteorological Institute (DMI) synoptic stations, inland Greenland Climate Network (GC-Net) and University of Wisconsin Automatic Weather Stations (AWSs), and two energy balance stations near the southern ice margin. The ECMWF analyses closely reproduce the seasonal patterns and interannual variations of much of the in situ data. Differences in the mean values of surface air pressure and temperature can mainly be ascribed to orography errors in the analyses' schemes, compared with the latest available accurate digital elevation model. Much of the GIS margin as modelled by ECMWF was too cold, on average by 4°C, and ECMWF precipitation averaged some 136% of the DMI station values. The misrepresentation of the (relatively) steep ice-sheet margin, which tends to be broadened and systematically over-elevated by several hundred metres, orographically reduced temperature and enhanced precipitation there in the ECMWF models. The cloud-cover comparison revealed not dissimilar annual mean cloud covers (ECMWF ,8%) but the ECMWF analyses had too little cloud and were too ,sunny' during the critical summer melt-season. ECMWF-modelled surface albedo in summer was ,11% lower than GC-Net values, which was mainly responsible for the disagreement of modelled surface short-wave radiation fluxes with observations. Model albedo and cloud errors need to be rectified if the analyses are to be used effectively to drive energy balance models of Greenland snowmelt. ECMWF wind speed averaged 66% (62%) of the DMI station (AWS) values. The validation results provide useful insights into how one can best improve the ECMWF Greenland climate data for use in glaciological and climatological studies. Copyright © 2001 Royal Meteorological Society [source] Is succession in wet calcareous dune slacks affected by free sulfide?JOURNAL OF VEGETATION SCIENCE, Issue 2 2003Erwin B. Adema van der Meijden (1996) for phanerogams; Schaminée et al. (1995) for syntaxa Abstract. Consequences of sulfide toxicity on succession in wet calcareous dune slacks were investigated. Sulfide may exert an inhibitory effect on dune slack plants, but several pioneer species exhibit ROL (Radial Oxygen Loss) and thereby protect themselves against free sulfide. Under oxic conditions free sulfide will be oxiginated to harmless sulfate. However, successive species when not capable of ROL may be sensitive to free sulfide and cannot invade the area. Therefore, the occurrence of free sulfide may have a stabilizing effect on the pioneer vegetation. Data on the vertical distribution of oxygen, redox and sulfide were collected in mesocosms with Littorella uniflora or Carex nigra, with and without microbial mats and compared to control mesocosms. Also, in situ data were collected in a dune slack on the Frisian Island of Texel. In the mesocosms, free sulfide was detected only at nighttime in C. nigra populated mesocosms and in unvegetated units, but not in L. uniflora vegetated mesocosms. In the field, sulfide and redox profiles showed distinct differences between the groundwater exfiltration and infiltration site of the dune slack. At the exfiltration site, sulfide was only occasionally found; in contrast, measurable amounts of free sulfide were regularly found at the infiltration site of the slack. Since Phragmites australis dominates in the infiltration site of the slack, the results suggest that free sulfide accelerate the succession, rather than slowing it down by the exclusion of some plant species. [source] Astronomical site selection: on the use of satellite data for aerosol content monitoringMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 2 2008A. M. Varela ABSTRACT The main goal of this work is to analyse new approaches to the study of the properties of astronomical sites. In particular, satellite data measuring aerosols have recently been proposed as a useful technique for site characterization and searching for new sites to host future very large telescopes. Nevertheless, these data need to be critically considered and interpreted in accordance with the spatial resolution and spectroscopic channels used. In this paper, we have explored and retrieved measurements from satellites with high spatial and temporal resolutions and concentrated on channels of astronomical interest. The selected data sets are the Ozone Monitoring Instrument on board the NASA Aura satellite and the Moderate Resolution Imaging Spectroradiometer on board the NASA Terra and Aqua satellites. A comparison of remote-sensing and in situ techniques is discussed. As a result, we find that aerosol data provided by satellites up to now are not reliable enough for aerosol site characterization, and in situ data are required. [source] Assessment of the impact of key terrestrial observing systems using DMI-HIRLAMTHE QUARTERLY JOURNAL OF THE ROYAL METEOROLOGICAL SOCIETY, Issue 633 2008Bjarne Amstrup Abstract Following a EUCOS decision to make an assessment of the impact on NWP forecasts of different components of the current observing systems in various combinations, a number of OSEs (Observing System Experiments) have been made by a number of NWP centres, with some running global models and some limited-area models. The Danish Meteorological Institute (DMI) carried out OSEs with the limited-area model HIRLAM. The following runs were made by DMI (two periods, one month each): (1) A baseline system (BL); (2) BL + all aircraft wind and temperature data; (3) BL + non-GUAN radiosonde winds; (4) BL + non-GUAN radiosonde temperature and wind; (5) BL + wind profiler; (6) as (4) + aircraft wind and temperature; (7) as (4) + non-GUAN radiosonde humidity; (8) as BL + all in situ data (full combined system); and (9) BL + E-AMDAR only (no ACARS and no traditional AIREPs). The lateral boundaries for these OSEs were provided by runs made by ECMWF, which was one of the centres to make OSEs with a global model. The main conclusions are that the radiosonde data are the most important data, closely followed by the aircraft data, and that aircraft data and radiosonde data are complementary and not redundant data. Furthermore the results show that it is important to have both wind and temperature profile data; wind data alone produce much poorer impact. Copyright © 2008 Royal Meteorological Society [source] A comparison of cloud-resolving model simulations of trade wind cumulus with aircraft observations taken during RICOTHE QUARTERLY JOURNAL OF THE ROYAL METEOROLOGICAL SOCIETY, Issue 624 2007S. J. Abel Abstract This paper presents results from simulations of trade wind cumulus with the Met Office Large Eddy Model (LEM) based on observed environmental profiles from the Rain In Cumulus over the Ocean (RICO) field experiment. Comparisons of updraught core parameters are made with the in situ data collected onboard the three research aircraft that participated in RICO. The default set-up of the LEM was unable to produce sufficient amounts of rainwater content when compared to measurements by the aircraft. As a main aim of RICO was to quantify the importance of precipitation in the trade cumulus regime, we test the sensitivity of the model to changes in the rain microphysics, the large-scale forcing, and horizontal resolution. By changing these model variables we are able to obtain reasonably good agreement with the observations of updraught core vertical velocity, cloud and rainwater contents. Furthermore, the LEM produces comparable surface precipitation rates to those derived from radar measurements during RICO in a previous study. This gives us some confidence in the ability of the LEM to simulate realistic precipitating trade cumulus. Copyright © 2007 Royal Meteorological Society [source] In situ kinetic modelling of intestinal efflux in rats: functional characterization of segmental differences and correlation with in vitro resultsBIOPHARMACEUTICS AND DRUG DISPOSITION, Issue 5 2007Isabel González-Alvarez Abstract The objective was to devise and apply a novel modelling approach to combine segmental in situ rat perfusion data and in vitro cell culture data, in order to elucidate the contribution of efflux in drug absorption kinetics. The fluoroquinolone CNV97100 was used as a model P-gp substrate. In situ intestinal perfusion was performed in rat duodenum, jejunum, ileum and colon to measure the influence of P-gp expression on efflux. Inhibition studies of CNV97100 were performed in the presence of verapamil, quinidine, cyclosporin A and p -aminohippuric acid. Absorption/efflux parameters were modelled simultaneously, using data from both in situ studies as well as in vitro studies. The maximal efflux velocity was modelled as a baseline value, corrected for each segment based on the expression level. CNV97100 passive diffusional permeability (Pdiff) and its affinity for the efflux carrier (Km) were assumed to be the same in all segments. The results indicate the new approach to combine in situ data and in vitro data succeed in yielding a unified, quantitative model for absorption/efflux. The model incorporated a quantitative relationship between P-gp expression level and the efflux functionality, both across in situ and in vitro systems, as well across different intestinal segments in the in situ studies. Permeability values decreased from duodenum to ileum in accordance with the increasing P-gp expression levels in rat intestine. The developed model reflects a strong correlation between in vitro and in situ results, including intrinsic differences in surface area. The successful application of a model approach to combine absorption data from two different experimental systems holds promise for future efforts to predict absorption results from one system to a second system. Copyright © 2007 John Wiley & Sons, Ltd. [source] | |||||||||||||