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Several Other (several + other)
Terms modified by Several Other Selected AbstractsProtein methylation in full length Chlamydomonas flagellaCYTOSKELETON, Issue 8 2009Roger D. Sloboda Abstract Post-translational protein modification occurs extensively in eukaryotic flagella. Here we examine protein methylation, a protein modification that has only recently been reported to occur in flagella [Schneider MJ, Ulland M, Sloboda RD.2008. Mol Biol Cell 19(10):4319,4327.]. The cobalamin (vitamin B12) independent form of the enzyme methionine synthase (MetE), which catalyzes the final step in methionine production, is localized to flagella. Here we demonstrate, using immunogold scanning electron microscopy, that MetE is bound to the outer doublets of the flagellum. Methionine can be converted to S-adenosyl methionine, which then serves as the methyl donor for protein methylation reactions. Using antibodies that recognize symmetrically or asymmetrically methylated arginine residues, we identify three highly methylated proteins in intact flagella: two symmetrically methylated proteins of about 30 and 40 kDa, and one asymmetrically methylated protein of about 75 kDa. Several other relatively less methylated proteins could also be detected. Fractionation and immunoblot analysis shows that these proteins are components of the flagellar axoneme. Immunogold thin section electron microscopy indicates that the symmetrically methylated proteins are located in the central region of the axoneme, perhaps as components of the central pair complex and the radial spokes, while the asymmetrically methylated proteins are associated with the outer doublets. Cell Motil. Cytoskeleton 2009. © 2009 Wiley-Liss, Inc. [source] DNA aptamers developed against a soman derivative cross-react with the methylphosphonic acid core but not with flanking hydrophobic groupsJOURNAL OF MOLECULAR RECOGNITION, Issue 3 2009John G. Bruno Abstract Twelve rounds of systematic evolution of ligands by exponential enrichment (SELEX) were conducted against a magnetic bead conjugate of the para -aminophenylpinacolylmethylphosphonate (PAPMP) derivative of the organophosphorus (OP) nerve agent soman (GD). The goal was to develop DNA aptamers that could scavenge GD in vivo, thereby reducing or eliminating the toxic effects of this dangerous compound. Aptamers were sequenced and screened in peroxidase-based colorimetric plate assays after rounds 8 and 12 of SELEX. The aptamer candidate sequences exhibiting the highest affinity for the GD derivative from round 8 also reappeared in several clones from round 12. Each of the highest affinity PAPMP-binding aptamers also bound methylphosphonic acid (MPA). In addition, the aptamer with the highest overall affinity for PAPMP carried a sequence motif (TTTAGT) thought to bind MPA based on previously published data (J. Fluoresc 18: 867,876, 2008). This sequence motif was found in several other relatively high affinity PAPMP aptamer candidates as well. In studies with the nerve agent GD, pre-incubation of a large molar excess of aptamer candidates failed to protect human butyrylcholinesterase (BuChE) from inhibition. With the aid of three-dimensional molecular modeling of the GD derivative it appears that a hydrophilic cleft sandwiched between the pinacolyl group and the p -aminophenyl ring might channel nucleotide interactions to the phosphonate portion of the immobilized GD derivative. However, bona fide GD free in solution may be repulsed by the negative phosphate backbone of aptamers and rotate its phosphonate and fluorine moieties away from the aptamer to avoid being bound. Future attempts to develop aptamers to GD might benefit from immobilizing the pinacolyl group of bona fide GD to enhance exposure of the phosphonate and fluorine to the random DNA library. Copyright © 2008 John Wiley & Sons, Ltd. [source] Further 2MASS mapping of hot dust in planetary nebulaeMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 4 2006J. P. Phillips ABSTRACT We have used 2 Micron All Sky Survey (2MASS) mapping results to investigate the distribution of hot dust continua in 12 planetary nebulae (PNe). The nature of this emission is unclear, but it is possible that where the continuum is extended, as is the case for M 1-12 and NGC 40, then the grains concerned may be very small indeed. The absorption of individual photons by such grains may lead to sharp spikes in temperature, as has previously discussed for several other such outflows. Other sources (such as MaC 1-4, He 2-25, B1 2-1 and K 3-15) appear to be relatively compact, and the high temperatures observed are understandable in terms of more normal heating processes. It is possible that the grains in these cases are experiencing high radiant flux levels. Finally, it is noted that whilst the core of M 2-2 appears to show hot grain emission, this is less the case for its more extended envelope. The situation may, in this case, be similar to that of NGC 2346, in which much of the emission is located within an unresolved nucleus. Similarly, it is noted that in addition to hot dust and gas thermal continua, the emission in the interior of NGC 40 may be enhanced through rotational,vibrational transitions of H2, and/or the 2p3P0,2s3S transition of He i. [source] Neon abundances in normal late-B and mercury,manganese starsMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 4 2000M. M. Dworetsky We make new non-local thermodynamic equilibrium calculations to deduce the abundances of neon from visible-region echelle spectra of selected Ne i lines in seven normal stars and 20 HgMn stars. We find that the best strong blend-free Ne line that can be used at the lower end of the effective temperature Teff range is ,6402, although several other potentially useful Ne i lines are found in the red region of the spectra of these stars. The mean neon abundance in the normal stars (log A=8.10) is in excellent agreement with the standard abundance of neon (8.08). However, in HgMn stars neon is almost universally underabundant, ranging from marginal deficits of 0.1,0.3 dex to underabundances of an order of magnitude or more. In many cases, the lines are so weak that only upper limits can be established. The most extreme example found is , Her with an underabundance of at least 1.5 dex. These underabundances are qualitatively expected from radiative acceleration calculations, which show that Ne has a very small radiative acceleration in the photosphere, and that it is expected to undergo gravitational settling if the mixing processes are sufficiently weak and there is no strong stellar wind. According to theoretical predictions, the low Ne abundances place an important constraint on the intensity of such stellar winds, which must be less than 10,14 M, yr,1 if they are non-turbulent. [source] Properties of scoring auctionsTHE RAND JOURNAL OF ECONOMICS, Issue 1 2008John Asker This article studies scoring auctions, a procedure commonly used to buy differentiated products: suppliers submit offers on all dimensions of the good (price, level of nonmonetary attributes), and these are evaluated using a scoring rule. We provide a systematic analysis of equilibrium behavior in scoring auctions when suppliers' private information is multidimensional (characterization of equilibrium behavior and expected utility equivalence). In addition, we show that scoring auctions dominate several other commonly used procedures for buying differentiated products, including menu auctions, beauty contests, and price-only auctions with minimum quality thresholds. [source] Inference in Spline-Based Models for Multiple Time-to-Event Data, with Applications to a Breast Cancer Prevention TrialBIOMETRICS, Issue 4 2003Kiros Berhane Summary. As part of the National Surgical Adjuvant Breast and Bowel Project, a controlled clinical trial known as the Breast Cancer Prevention Trial (BCPT) was conducted to assess the effectiveness of tamoxifen as a preventive agent for breast cancer. In addition to the incidence of breast cancer, data were collected on several other, possibly adverse, outcomes, such as invasive endometrial cancer, ischemic heart disease, transient ischemic attack, deep vein thrombosis and/or pulmonary embolism. In this article, we present results from an illustrative analysis of the BCPT data, based on a new modeling technique, to assess the effectiveness of the drug tamoxifen as a preventive agent for breast cancer. We extended the flexible model of Gray (1994, Spline-based test in survival analysis, Biometrics50, 640,652) to allow inference on multiple time-to-event outcomes in the style of the marginal modeling setup of Wei, Lin, and Weissfeld (1989, Regression analysis of multivariate incomplete failure time data by modeling marginal distributions, Journal of the American Statistical Association84, 1065,1073). This proposed model makes inference possible for multiple time-to-event data while allowing for greater flexibility in modeling the effects of prognostic factors with nonlinear exposure-response relationships. Results from simulation studies on the small-sample properties of the asymptotic tests will also be presented. [source] Reactions of {4-[Bis(2-chloroethyl)amino]phenyl}acetic Acid (Phenylacetic Acid Mustard) with 2,-DeoxyribonucleosidesCHEMISTRY & BIODIVERSITY, Issue 3 2007Diana Florea-Wang Abstract Phenylacetic acid mustard (PAM; 2), a major metabolite of the anticancer agent chlorambucil (CLB; 1), was allowed to react with 2,-deoxyadenosine (dA), 2,-deoxyguanosine (dG), 2,-deoxycytidine (dC), 2,-deoxy-5-methylcytidine (dMeC), and thymidine (T) at physiological pH (cacodylic acid, 50% base). The reactions were followed by HPLC and analyzed by HPLC/MS and/or 1H-NMR techniques. Although the predominant reaction observed was hydrolysis of PAM, 2 also reacted with various heteroatoms of the nucleosides to give a series of products: compounds 5,31. PAM (2) was found to be hydrolytically slightly more stable than CLB (1). The principal reaction sites of 2 with dA, dG, and with all pyrimidine nucleosides were N(1), N(7), and N(3), resp. Also, several other adducts were detected and characterized. There was no significant difference in the reactivity of 1 and 2 with dG, dA or T, but the N(3) dC,PAM adduct was deaminated easier than the corresponding CLB derivative. The role of PAM,2,-deoxyribonucleoside adducts on the cytotoxic and mutagenic properties of CLB (1) is discussed. [source] | ||||||||||