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Several Different Methods (several + different_methods)
Selected AbstractsThe motility of glioblastoma tumour cells is modulated by intracellular cofilin expression in a concentration-dependent mannerCYTOSKELETON, Issue 3 2005Celestial T. Yap Abstract The invasive behaviour of tumour cells has been attributed in part to dysregulated cell motility. Members of the ADF/Cofilin family of actin-binding proteins are known to increase microfilament dynamics by increasing the rate at which actin monomers leave the pointed end of the filament and by a filament-severing activity. As depolymerisation is a rate-limiting step in actin dynamics, ADF/Cofilins are suspected to facilitate the motility of cells. To test this, we investigated the influence of cofilin on tumour motility by transient and stably overexpressing cofilin in the human glioblastoma cell line, U373 MG. Several different methods were used to ascertain the level of cofilin in overexpressing clones and this was correlated with their rate of random locomotion. A biphasic relationship between cofilin level and locomotory rate was found. Clones that displayed a moderate amount of overproduction of cofilin were found to have increased rates of locomotion approximately linear to the overproduction of cofilin up to an optimal cofilin level of about 4.5 times that of wild type cells at which the cells were almost twice as fast. However, clones producing more than this optimal amount were found to locomote at progressively reduced speeds. Cells that overexpress cofilin have reduced stress fibres compared to control cells showing that the excess cofilin affects the actin cytoskeleton. We conclude that overexpression of cofilin enhances the motility of glioblastoma tumour cells in a concentration-dependent fashion, which is likely to contribute to their invasiveness. Cell Motil. Cytoskeleton 60:153,165, 2005. © 2005 Wiley-Liss, Inc. [source] Use of Shade Guides for Color Measurement in Tooth-Bleaching StudiesJOURNAL OF ESTHETIC AND RESTORATIVE DENTISTRY, Issue 2003WILLIAM D. BROWNING DDS ABSTRACT Several different methods are used to measure tooth color in bleaching studies. The ADA Acceptance Program Guidelines for Home Use Tooth Whitening Products specify the use of a value-oriented shade guide and/or electronic color measurement devices. Since people perceive color differently, shade guides are a subjective measure. Differences between raters and by the same rater are well documented in the dental literature. The purposes of this article will be to discuss the advantages and disadvantages using shade guides to measure color change related to tooth whitening, and to evaluate the correlation of data gathered from the use of shade guides to electronic color measurement devices. Using an order published by the manufacturer, both the TRUBYTE® Bioform and Vita Classical guides can be arranged by value. A study by O'Brien demonstrated however, that the order is flawed and the change in brightness from tab to tab varies greatly. Despite these disadvantages, a review of data from several clinical trials demonstrates that Vita Classical shade guide data is consistent with data gathered using electronic color measurements. Furthermore, the O'Brien data can be used to make both these guides better measurement systems. The ADA Certification program standards define the degree of overall color change that should be considered clinically important. This issue is as critical as the measurement system used. Reporting color changes that are neither detectable to the human eye nor considered by the public to be important offers the profession little usable information. Given that any standard for color change during bleaching must relate to the abilities of the human eye, it is the conclusion of the author that shade guides should remain a critical element of any bleaching study. CLINICAL SIGNIFICANCE Clinicians are frequently exposed to reports of bleaching agents that have been shown to result in a change of 6, 7, 8, etc., tabs. Without understanding the limitations of the shade guide used, reports of a specific shade tab change are of little use and may actually be misleading. [source] Spatial prediction of river channel topography by krigingEARTH SURFACE PROCESSES AND LANDFORMS, Issue 6 2008Carl J. Legleiter Abstract Topographic information is fundamental to geomorphic inquiry, and spatial prediction of bed elevation from irregular survey data is an important component of many reach-scale studies. Kriging is a geostatistical technique for obtaining these predictions along with measures of their reliability, and this paper outlines a specialized framework intended for application to river channels. Our modular approach includes an algorithm for transforming the coordinates of data and prediction locations to a channel-centered coordinate system, several different methods of representing the trend component of topographic variation and search strategies that incorporate geomorphic information to determine which survey data are used to make a prediction at a specific location. For example, a relationship between curvature and the lateral position of maximum depth can be used to include cross-sectional asymmetry in a two-dimensional trend surface model, and topographic breaklines can be used to restrict which data are retained in a local neighborhood around each prediction location. Using survey data from a restored gravel-bed river, we demonstrate how transformation to the channel-centered coordinate system facilitates interpretation of the variogram, a statistical model of reach-scale spatial structure used in kriging, and how the choice of a trend model affects the variogram of the residuals from that trend. Similarly, we show how decomposing kriging predictions into their trend and residual components can yield useful information on channel morphology. Cross-validation analyses involving different data configurations and kriging variants indicate that kriging is quite robust and that survey density is the primary control on the accuracy of bed elevation predictions. The root mean-square error of these predictions is directly proportional to the spacing between surveyed cross-sections, even in a reconfigured channel with a relatively simple morphology; sophisticated methods of spatial prediction are no substitute for field data. Copyright © 2007 John Wiley & Sons, Ltd. [source] How close is close enough?JOURNAL OF POLICY ANALYSIS AND MANAGEMENT, Issue 3 2007Evaluating propensity score matching using data from a class size reduction experiment In recent years, propensity score matching (PSM) has gained attention as a potential method for estimating the impact of public policy programs in the absence of experimental evaluations. In this study, we evaluate the usefulness of PSM for estimating the impact of a program change in an educational context (Tennessee's Student Teacher Achievement Ratio Project [Project STAR]). Because Tennessee's Project STAR experiment involved an effective random assignment procedure, the experimental results from this policy intervention can be used as a benchmark, to which we compare the impact estimates produced using propensity score matching methods. We use several different methods to assess these nonexperimental estimates of the impact of the program. We try to determine "how close is close enough," putting greatest emphasis on the question: Would the nonexperimental estimate have led to the wrong decision when compared to the experimental estimate of the program? We find that propensity score methods perform poorly with respect to measuring the impact of a reduction in class size on achievement test scores. We conclude that further research is needed before policymakers rely on PSM as an evaluation tool. © 2007 by the Association for Public Policy Analysis and Management [source] Comparison of methods for teaching clinical skills in assessing and managing drug-seeking patientsMEDICAL EDUCATION, Issue 4 2000Taverner Aims New medical graduates lack clinical skills in assessing and managing patients seeking drugs of dependence. This study compares the effectiveness of three different clinical skills training methods, with similar content, which were developed to teach these skills to senior medical students. Methods A preliminary survey indicated that common problems seen by primary care practitioners included both new and previously known patients seeking either benzodiazepines or opiates. The common content of the teaching was determined from this survey. A didactic small group tutorial (DT), a video-based tutorial (VBT) using professional actors, and a computer-aided instruction package using digitized video (CAI) were developed with this common content, and trialled with undergraduate medical students over 2 years in a parallel-group design. Outcome was assessed by student feedback, performance on a case-based written examination and by a structured evaluation of interviews with simulated patients requesting drugs. Comparison was also made between methods on the basis of knowledge tests. Results No difference was seen in written examination and simulated patient outcomes between the three groups. However, the VBT was thought by the students to be preferable to other methods. The estimated development costs of CAI were higher, but total costs over a 6-year period were lower than for the DT and VBT. The results suggest that clinical skills can be taught equally effectively through several different methods. Collaboration between institutions in the development of widely applicable CAI tools should be an efficient and economical mode of teaching with a wide range of applications. [source] | ||||||||||