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Second Strand (second + strand)
Selected AbstractsThe Tension in Wittgenstein's Diagnosis of ScepticismDIALECTICA, Issue 3 2000Reid Buchanan I argue that Wittgenstein's rejection of scepticism in On Certainty rests on the view that epistemic concepts such as,doubt,,knowledge',,justification'and so on, cannot be intelligibly applied to the common sense propositions that traditional sceptical arguments appear to undermine. I detect two strands in On Certainty in support of this view. I attempt to show that neither of these strands adequately establishes the thesis, and that they point to a tension in Wittgenstein's treatment of scepticism. I argue that the first strand is dogmatic, but accords with the constraints of Wittgenstein's method, while the second strand avoids the dogmatism of the first at the cost of violating these constraints. [source] Synthesis and Triple-Helix-Stabilization Properties of Branched Oligonucleotides Carrying 8-Aminoadenine MoietiesHELVETICA CHIMICA ACTA, Issue 2 2004Anna Aviño The synthesis of several branched oligonucleotides, i.e., of the parallel hairpins 5,8 and the Y-shaped 9 is described, together with their use in the formation of pyrimidine,pyrimidine,purine triple helices. Special attention was paid to the optimization of the assembly of the second strand from asymmetric branching molecules. The presence of 8-aminoadenine moieties in the WatsonCrick purine strand and 2,- O -methyl-RNA in the Hoogsteen pyrimidine strand produced strong stabilization of the triplex. [source] Development of a consensus microarray method for identification of some highly pathogenic virusesJOURNAL OF MEDICAL VIROLOGY, Issue 11 2009Kang Xiao-Ping Abstract Some highly pathogenic viruses, such as Chikungunya virus, Japanese encephalitis virus, Yellow fever virus, Dengue virus, Hanta virus, SARS-CoV, and H5N1 avian influenza virus can cause severe infectious diseases. However, the consensus method for detecting these viruses has not been well established. A rapid and sensitive microarray approach for detection of these viruses and a panel of specific probes covering nine genera and 16 virus species were designed. 70-mer oligonucleotides were used at the genus level and 50-mer oligonucleotides were at the species level, respectively. To decrease the interference of the host genome in hybridization, the consensus genus primers were designed and used to reverse transcribe only virus genome. The synthesis of the second strand was carried out with a random primer sequence (5,-GTTTCCCAGTAGGTCTCNNNNNNNN-3,). The amplified products were labeled and processed for microarray analyses. This microarray-based method used the highly conserved consensus primers to synthesize specifically the virus cDNA and could identify effectively Chikungunya virus, Japanese encephalitis virus, Yellow fever virus, Dengue virus, Tick borne encephalitis virus, and H5N1 avian influenza virus. Using this method, one unknown virus isolated from pig brain in Shanxi Province, China was identified. This method may have an important potential application for the diagnosis of virus infection. J. Med. Virol. 81:1945,1950, 2009. © 2009 Wiley-Liss, Inc. [source] Destabilizing effect of a fluorouracil extra base in a hybrid RNA duplex compared with bromo and chloro analoguesACTA CRYSTALLOGRAPHICA SECTION D, Issue 11 2001William Cruse In the presence of cobalt, rhodium or iridium hexammine salts, the RNA/DNA hybrid r-GCUUCGGC-dXU (with X = F, Cl or Br) crystallizes as a double-stranded helix with four consecutive G,U and C,U mismatches. The deoxy chloro- and bromouracil derivatives are isomorphous, space group C2, unit-cell parameters a = 53.80, b = 19.40, c = 50.31,Å, , = 109.9°, with the same infinite helix arrangement in the packing along the c axis with one extra DNA halogenouracil base included in the stacking. However, the fluorouracil derivative, with unit-cell parameters a = 53.75, b = 19.40, c = 45.84,Å, , = 105.7°, is not isomorphous. The corresponding extra DNA base dFU of the second strand is ejected out of the helical stack, leading to a shortening of the c axis. The specific destabilization of the fluorouracil for the duplex building is analyzed in terms of the polarization effect of the halogen atom attached to the 3,-terminal base that modulates its interactions. [source] | ||||||||||