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Sex Chromosome System (sex + chromosome_system)

Distribution by Scientific Domains

Life Sciences	100%

Selected Abstracts

Rapid assessment of the sex of codling moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) eggs and larvae

JOURNAL OF APPLIED ENTOMOLOGY, Issue 4 2009
I. Fuková
Abstract Two different methods were tested to identify the sex of the early developmental stages of the codling moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) with a WZ/ZZ (female/male) sex chromosome system. First, it was shown that the sex of all larval stages can be easily determined by the presence or absence of sex chromatin, which is formed by the female-specific W chromosome in interphase nuclei. This trait can also be used to identify the sex of newly hatched larvae but it does require care and accuracy. Secondly, a new sexing technique was developed based on a molecular marker of the codling moth W chromosome. Flanking regions of an earlier described W-specific sequence (CpW2) were isolated and sequenced and a 2.74 kb sequence (CpW2- EcoRI), specific for the W chromosome, was obtained. Several PCR tests were conducted, which confirmed that the CpW2- EcoRI sequence is a reliable marker for the sex identification in codling moth samples of different geographical origin. In addition, a fragment of a codling moth gene, period (Cpper) was isolated and sequenced. Results of southern hybridization of the Cpper probe with female and male genomic DNA suggested that the Cpper gene is located on the Z chromosome. Then a multiplex PCR assay was developed, which co-amplified the CpW2- EcoRI sequence to identify the W chromosome and the Z-linked Cpper sequence, which served as a positive control of accurate processing of tested samples. The multiplex PCR provides an easy and rapid identification of the sex of embryos and early larval instars of the codling moth. [source]

Sex chromatin and sex chromosome systems in nonditrysian Lepidoptera (Insecta)

JOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH, Issue 2 2000
V. A. Lukhtanov
Eleven representatives of the superorder Amphiesmenoptera (Trichoptera + Lepidoptera) were examined for sex chromatin status. Three species represent stenopsychoid, limnephiloid and leptoceroid branches of the Trichoptera; eight species belong to the primitive, so-called nonditrysian Lepidoptera and represent the infra-orders Zeugloptera, Dacnonypha, Exoporia, Incurvariina, Nepticulina and Tischeriina. The female-specific sex chromatin body was found in the interphase somatic nuclei of Tischeria ekebladella (Bjerkander 1795) (Lepidoptera, Tischeriina). The sex chromatin was absent in all investigated Trichoptera species as well as in all representatives of the nonditrysian Lepidoptera except Tischeria ekebladella. The sex chromosome mechanism of Limnephilus lunatus Curtis 1834 (Trichoptera, Limnephilidae) is Z/ZZ. The sex chromosome mechanism of Tischeria ekebladella (Lepidoptera, Tischeriina) is ZW/ZZ including the W chromosome as the largest element in the chromosome set. The data obtained support the hypothesis that the Z/ZZ sex chromosome system, the female heterogamety and the absence of the sex chromatin body in interphase nuclei are ancestral traits in the superorder Amphiesmenoptera. These ancestral characters are probably kept constant in all the Trichoptera and in the most primitive Lepidoptera. The W sex chromosome and the sex chromatin evolved later in the nonditrysian grade of the Lepidoptera. It is proposed that the sex chromatin is a synapomorphy of Tischeriina and Ditrysia. [source]

Karyotype differentiation in Chromaphyosemion killifishes (Cyprinodontiformes, Nothobranchiidae): patterns, mechanisms, and evolutionary implications

BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, Issue 1 2008
MARTIN VÖLKER
Chromaphyosemion killifishes are a karyotypically highly diverse group of small, sexually dimorphic fishes living in rainforest rivulets in tropical West and Central Africa. In the present study, we used various chromosome banding and staining techniques to analyse the karyotypes of 13 populations representing seven described species (Chromaphyosemion loennbergii, Chromaphyosemion punctulatum, Chromaphyosemion splendopleure, Chromaphyosemion volcanum, Chromaphyosemion malumbresi, Chromaphyosemion melanogaster, Chromaphyosemion bitaeniatum) and two undescribed forms (Chromaphyosemion cf. lugens, Chromaphyosemion sp. Rio Muni GEMHS00/41). Diploid chromosome numbers (2 n) and the number of chromosome arms (NF) ranged from 2 n = 24 in C. malumbresi to 2 n = 40 in C. bitaeniatum and from NF = 40 in C. volcanum and C. cf. lugens to NF = 54 in one population of C. loennbergii. A tentative XX/XY sex chromosome system was revealed in C. loennbergii, C. melanogaster, C. malumbresi, and Chromaphyosemion sp. Rio Muni GEMHS00/41. Mapping cytogenetic data for all described Chromaphyosemion species onto a recently published mitochondrial DNA phylogeny revealed a complex pattern of chromosomal evolution with several independent reductions of 2 n and independent modifications of NF and nucleolus organizer region phenotypes. Together with the results of preliminary crossing and mate choice experiments, the cytogenetic and molecular phylogenetic data suggest that, contrary to previous hypotheses, chromosomal rearrangements are probably not the most important and certainly not the only factor driving speciation in Chromaphyosemion killifishes. © 2008 The Linnean Society of London, Biological Journal of the Linnean Society, 2008, 94, 143,153. [source]