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Semen Collection (semen + collection)
Selected AbstractsSemen collection in an Asian elephant (Elephas maximus) under combined physical and chemical restraintAUSTRALIAN VETERINARY JOURNAL, Issue 10 2007TJ PORTAS No abstract is available for this article. [source] Cryopreservation of semen from a stallion with seminal vesiculitisEQUINE VETERINARY EDUCATION, Issue 5 2010L. C. Fennell Summary A 6-year-old Warmblood stallion was admitted for semen collection and cryopreservation. On the seventh and subsequent collection days semen samples were contaminated with purulent debris. A diagnosis of seminal vesiculitis was made following ultrasonography and endoscopy of the seminal vesicles. The stallion was treated with systemic and topical antimicrobial therapy and, although this did not cure the condition, subsequent ejaculates were suitable for cryopreservation. [source] Strategies for the management of donkey jacks in intensive breeding systemsEQUINE VETERINARY EDUCATION, Issue 12 2009I. F. Canisso Summary Donkeys are bred throughout the world and often play an important role in agriculture. They are also bred to produce mules. Traditionally, jacks are considered challenging to breed in domestic conditions, whether for natural breeding or semen collection using either jennies or mares. The donkey's natural sexual behaviour significantly differs from that of other domestic animals. This presents challenges for in-hand donkey breeding, particularly on mule studs where normally only jacks and mares are kept for breeding. This article describes some of the authors' observations on sexual behaviour in donkeys and practical experience of some of the strategies employed to apply this knowledge to breeding management, in order to improve the success of using donkeys for both natural service and semen collection. [source] Semen quality in fertile US men in relation to geographical area and pesticide exposureINTERNATIONAL JOURNAL OF ANDROLOGY, Issue 1 2006Shanna H. Swan Summary We conducted the first US study to compare semen quality among study centres using standardized methods and strict quality control. We present data on semen quality in partners of 493 pregnant women recruited through prenatal clinics in four US cities during 1999,2001. Sperm concentration, semen volume and motility were determined at the centres and morphology was assessed at a central laboratory. While between-centre differences in sperm morphology and sample volume were small, sperm concentration and motility were significantly reduced in Columbia, MO (MO) relative to men in New York, NY, Minneapolis, MN and Los Angeles, CA; total number of motile sperm was 113 × 106 in MO and 162, 201 and 196 × 106 in CA, MN and NY respectively. Differences among centres remained significant in multivariate models that controlled for abstinence time, semen analysis time, age, race, smoking, history of sexually transmitted disease and recent fever (all p -values <0.01). We hypothesized that poorer sperm concentration and motility in MO men relative to other centres might be related to agricultural pesticides that are commonly used in the mid-west. We investigated this hypothesis by conducting a nested case,control study within the MO cohort. We selected 25 men in this cohort for whom all semen parameters (concentration, % normal morphology and % motile) were low as cases and an equal number of men for whom all semen parameters were within normal limits as controls. We measured metabolites of eight non-persistent, current-use pesticides in urine samples the men had provided at the time of semen collection. Pesticide metabolite levels were elevated in cases compared with controls for the herbicides alachlor and atrazine, and for the insecticide diazinon (2-isopropoxy-4-methyl-pyrimidinol) (p -values for Wilcoxon rank test = 0.0007, 0.012, and 0.0004 for alachlor, atrazine and diazinon respectively). Men with higher levels of alachlor or diazinon were significantly more likely to be cases than men with low levels [odds ratios (OR) = 30.0, 16.7 for alachlor and diazinon respectively], as were men with atrazine over the limit of detection (OR = 11.3). These associations between current-use pesticides and reduced semen quality suggest that agricultural chemicals may have contributed to the reduced semen quality seen in fertile men from mid-Missouri. [source] Quality Parameters for Alpaca (Vicugna pacos) Semen are Affected by Semen Collection ProcedureREPRODUCTION IN DOMESTIC ANIMALS, Issue 4 2010KM Morton Contents Artificial insemination (AI) is poorly developed in camelids owing to the difficulty in collecting high quality semen and the highly viscous nature of the semen. Semen collected by artificial vagina (AV) is often of low quality and must be improved before any further development of AI technology can occur. The present study investigated the effects of adding a cervix-like stricture to the AV, presence of females, collecting semen into Androhep®, skim-milk or Tris diluents, and catalase supplementation (0, 100, 200 or 600 units/ml) of Tris diluent on alpaca semen quality parameters. The addition of a cervix-like stricture increased mating length (p < 0.05), whilst the presence of females during semen collection did not improve semen quality parameters (p > 0.05). Collection of semen into Tris diluent improved sperm motility (58.0 ± 11.9%) compared with the control (34.0 ± 10.8%; p < 0.05), Androhep® (33.5 ± 10.7%) and skim-milk diluents (28.2 ± 10.4%). Semen viscosity was reduced by collection into Androhep® (4.6 ± 1.7 mm) and skim-milk diluents (3.6 ± 1.3 mm) compared with Tris diluent (5.7 ± 2.1 mm) and no collection medium (9.3 ± 3.5 mm; p < 0.05). Tris diluent supplemented with 100, 200 or 600 units/ml catalase increased semen viscosity (5.0 ± 3.2 and 4.9 ± 3.2 mm). Collection of alpaca semen by AV into Tris diluent increased semen quality facilitating further development of AI technology in alpacas. [source] Assessment of sperm quality, DNA integrity and cryopreservation protocols in men diagnosed with testicular and systemic malignanciesANDROLOGIA, Issue 6 2009T. M. Said Summary Men diagnosed with malignancy are often referred for semen banking to preserve their fertility prior to cancer treatment. The chances of cancer patients for achieving future fecundity will be determined by the sperm quality including the integrity of the genomic material in the frozen samples. The objectives of this study were to compare the sperm quality and DNA integrity in men diagnosed with testicular and systemic malignancies before receiving treatment and to identify the optimum cryopreservation protocol for their samples including a remote semen collection option. In comparison with fertile donors, patients with testicular malignancies had significantly lower sperm concentration, while both testicular and systemic malignancy patients had significantly lower sperm motility and cryosurvival rates. In addition, the SCSA defined DNA fragmentation index was significantly higher in patients with testicular and systemic malignancies compared with fertile donors. It was noted that the extent of deterioration in sperm quality and DNA integrity seen in cancer patients did not reach the previously defined statistical threshold for impaired fertility. Freezing spermatozoa with the seminal plasma offers the highest protection against cryo-injury. Nevertheless, remote semen collection can still be used as it yields adequate results. [source] |