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Schisandrin B (schisandrin + b)

Distribution by Scientific Domains

Chemistry	75%

Selected Abstracts

Highly sensitive determination of Schisandrin and Schisandrin B in plasma of rats after administration of Wurenchun (Fructus Schisandrae Chinensis Extracts) preparations by LC-ESI-MS/MS

BIOMEDICAL CHROMATOGRAPHY, Issue 6 2010
Jingling Tang
Abstract A sensitive and specific method based on liquid chromatography-tandem mass spectrometry using electrospray ionization (LC-ESI-MS/MS) has been developed for the determination of Schisandrin and Schisandrin B in rat plasma. A 100,,L plasma sample was extracted by methyl tert-butyl ether after spiking the samples with nimodipine (internal standard) and performed on an XTerra®MS-C18 column (150,mm × 2.1,mm, 3.5,,m) with the mobile phase of acetonitrile,water,formic acid (80:20:0.2, v/v) at a flow rate of 0.2,mL/min in a run time of 8.5,min. The lower limit of quantification of the method was 40,ng/mL for Schisandrin and 20,ng/mL for Schisandrin B. The method showed reproducibility with intra-day and inter-day precision of less than 13.8% RSD, as well as accuracy, with inter- and intra-assay accuracies between 93.5 and 107.2%. Finally, the LC-ESI-MS/MS method was successfully applied to study the pharmacokinetics of Schisandrin and Schisandrin B in rats after administration of Wurenchun commercial formulations to rats. Copyright © 2009 John Wiley & Sons, Ltd. [source]

Development of a quality evaluation method for Fructus schisandrae by pressurized capillary electrochromatography

JOURNAL OF SEPARATION SCIENCE, JSS, Issue 3 2007
Jiajing Wang
Abstract A pressurized CEC (pCEC) method with postcolumn detection cell had been developed for quantifying the lignans from Fructus schisandrae extracts. The effects of different experimental conditions, such as the ACN content of the mobile phase, the concentration and pH of the buffer, the applied voltage, and the supplementary pressure were studied. Five lignans (schisandrin, gomisin A, schisantherin C, deoxyschizandrin, schisandrin B) were baseline separated using a mobile phase of ACN-phosphate buffer (pH 5.4; 5 mM) (40 : 60 v/v) under ,4 kV applied voltage. The method showed the satisfactory retention time and peak area repeatability. The calibration curves were linear in the range 50.0,1000.0 ,g/mL for schisandrin, 20.0,500.0 ,g/mL for gomisin A, 10.0,200.0 ,g/mL for schisantherin C, 20.0,500.0 ,g/mL for deoxyschizandrin, and 20.0,500.0 ,g/mL for schisandrin B. The correlation coefficients were between 0.9978 and 0.9989. With this pCEC system, fingerprints of F. schisandrae were preliminarily established to distinguish two members S. chinensis (Turcz.) Baill. and S. sphenanthera Rehd. Et Wils. of F. schisandrae by characteristic peaks, and evaluate the quality of various sources of raw materials by determining the contents of the five lignans. [source]

Schisandrin B stereoisomers protect against hypoxia/reoxygenation-induced apoptosis and associated changes in the Ca2+ -induced mitochondrial permeability transition and mitochondrial membrane potential in AML12 hepatocytes

PHYTOTHERAPY RESEARCH, Issue 11 2009
Po Yee Chiu
Abstract The effects of the schisandrin B stereoisomers, (±), -schisandrin [(±), -Sch] and (,)schisandrin B [(,)Sch B], on hypoxia/reoxygenation-induced apoptosis were investigated in AML12 hepatocytes. Changes in cellular reduced glutathione (GSH) levels, Ca2+ -induced mitochondrial permeability transitions (MPTs) and mitochondrial membrane potentials (,,m values) were also examined in (±), -Sch- and (,)Sch B-treated cells, without or with hypoxia/reoxygenation challenge. The (±), -Sch/(,)Sch B pretreatments (2.5,5.0 µm) protected against hypoxia/reoxygenation-induced apoptosis in AML12 cells in a concentration-dependent manner, with the (,)Sch B effect being more potent. Drug antiapoptotic effects were further evidenced by suppression of hypoxia/reoxygenation-induced mitochondrial cytochrome c release and subsequent cleavage of caspase 3 and poly-ADP-ribose polymerase by (,)Sch B pretreatment. Whereas hypoxia/reoxygenation challenge increased the extent of Ca2+ -induced MPT pore opening, and ,,m, in AML12 hepatocytes, cytoprotection afforded by (±), -Sch/(,)Sch B pretreatment against hypoxia/reoxygenation-induced apoptosis was associated with a decreased sensitivity to Ca2+ -induced MPT and an increased ,,m in both unchallenged and challenged cells, compared with the drug-free control. The results indicate that (±), -Sch/(,)Sch B pretreatment protected against hypoxia/reoxygenation-induced apoptosis in AML12 hepatocytes and that the cytoprotection afforded by (±), -Sch/(,)Sch B may at least in part be mediated by a decrease in sensitivity to Ca2+ -induced MPT, which may in turn result from enhancement of cellular GSH levels by drug pretreatments. Copyright © 2009 John Wiley & Sons, Ltd. [source]