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Average Polymorphic Information Content (average + polymorphic_information_content)
Selected AbstractsTetranucleotide microsatellites from the loggerhead sea turtle (Caretta caretta)MOLECULAR ECOLOGY RESOURCES, Issue 5 2007BRIAN M. SHAMBLIN Abstract We describe primers and polymerase chain reaction conditions to amplify 15 tetranucleotide microsatellite loci from the loggerhead sea turtle (Caretta caretta). The primers were tested on 30 individuals that nested along the Georgia, USA coast. The primer pairs developed in this study yielded an average of 13.9 alleles per locus (range of 10,21), an average observed heterozygosity of 0.91 (range 0.79,1.00), and an average polymorphic information content of 0.88 (range 0.84,0.92). [source] Tetranucleotide, trinucleotide, and dinucleotide loci from the bobcat (Lynx rufus)MOLECULAR ECOLOGY RESOURCES, Issue 2 2005BRANT C. FAIRCLOTH Abstract We describe primers and polymerase chain reaction (PCR) conditions to amplify four dinucleotide, one trinucleotide, and three tetranucleotide microsatellite DNA loci from the bobcat (Lynx rufus). The primers were tested on 22 individuals collected from a population located within southwestern Georgia (USA). The primer pairs developed in this study yielded an average of 7.4 alleles per locus (range four to 10), an average observed heterozygosity of 0.60 (range 0.40 to 0.76), and an average polymorphic information content of 0.70 (range 0.51 to 0.78). [source] Development of polymorphic expressed sequence tag-derived microsatellites for the extension of the genetic linkage map of the black tiger shrimp (Penaeus monodon)ANIMAL GENETICS, Issue 4 2006C. Maneeruttanarungroj Summary In this study, microsatellite markers were developed for the genetic linkage mapping and breeding program of the black tiger shrimp Penaeus monodon. A total of 997 unique microsatellite-containing expressed sequence tags (ESTs) were identified from 10 100 EST sequences in the P. monodon EST database. AT-rich microsatellite types were predominant in the EST sequences. Homology searching by the blastn and blastx programs revealed that these 997 ESTs represented 8.6% known gene products, 27.8% hypothetical proteins and 63.6% unknown gene products. Characterization of 50 markers on a panel of 35,48 unrelated shrimp indicated an average number of alleles of 12.6 and an average polymorphic information content of 0.723. These EST microsatellite markers along with 208 other markers (185 amplified fragment length polymorphisms, one exon-primed intron-crossing, six single strand conformation polymorphisms, one single nucleotide polymorphism, 13 non-EST-associated microsatellites and two EST-associated microsatellites) were analysed across the international P. monodon mapping family. A total of 144 new markers were added to the P. monodon maps, including 36 of the microsatellite-containing ESTs. The current P. monodon male and female linkage maps have 47 and 36 linkage groups respectively with coverage across half the P. monodon genome. [source] Characterization of 18 polymorphic microsatellite loci in the red-crowned crane (Grus japonensis), an endangered birdANIMAL SCIENCE JOURNAL, Issue 4 2010Hongfei ZOU ABSTRACT Red-crowned cranes (Grus japonensis) were classified as an endangered species by the International Union for Conservation of Nature, but recently, their population has decreased dramatically. For the purpose of conserving this endangered species, 18 microsatellite markers were developed, including 12 newly isolated ones from a genomic library and 6 modified from another crane species. The markers were characterized in 26 red-crowned cranes. As a result, these markers displayed 3,13 alleles, and the observed and expected heterozygosities ranged from 0.462 to 1.000 and from 0.483 to 0.884, respectively. The marker suite averaged 6.390 alleles per locus with an average polymorphic information content of 0.631. The combined exclusion probability (PE-1) was 0.9985, and the combined exclusion probability (PE-2) was 0.9999. Three of the 18 microsatellite loci presented significant deviations from Hardy,Weinberg Equilibrium (P < 0.05), likely due to sampling bias and unknown founder relatedness in a semi-free population. Our results show that microsatellite loci can provide a standard protocol for genetic information in red-crowned crane populations upon which strategies for effective conservation and management may be based. [source] | ||||||||||