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Sample Manipulation (sample + manipulation)
Selected AbstractsDetermination of Diazepam, Temazepam and Oxazepam at the Lead Film Electrode by Adsorptive Cathodic Stripping VoltammetryELECTROANALYSIS, Issue 17-18 2010Katarzyna Tyszczuk Abstract The determination of psychoactive 1,4-benzodiazepine drugs is of relevant interest in clinical, biomedical areas. Therefore a highly sensitive and simple voltammetric method for the determination of temazepam, diazepam and oxazepam at an in situ plated lead film electrode was developed. The method was successfully applied to the determination of diazepam and temazepam in pharmaceutical formulations with minimum sample manipulation and oxazepam in human urine samples without any separation steps. The determinations of oxazepam in human urine samples were performed in a flow system. Therefore a previous extraction procedure was not necessary to separate the active compound before its determination. [source] An accessible micro-capillary electrophoresis device using surface-tension-driven flowELECTROPHORESIS, Issue 9 2009Swomitra K. Mohanty Abstract We present a rapidly fabricated micro-capillary electrophoresis chip that utilizes surface-tension-driven flow for sample injection and extraction of DNA. Surface-tension-driven flow (i.e. passive pumping) [G. M. Walker et al., Lab. Chip. 2002, 2, 131,134] injects a fixed volume of sample that can be predicted mathematically. Passive pumping eliminates the need for tubing, valves, syringe pumps, and other equipment typically needed for interfacing with microelectrophoresis chips. This method requires a standard micropipette to load samples before separation, and remove the resulting bands after analysis. The device was made using liquid phase photopolymerization to rapidly fabricate the chip without the need of special equipment typically associated with the construction of microelectrophoresis chips (e.g. cleanroom) [A. K. Agarwal et al., J. Micromech. Microeng. 2006, 16, 332,340; S. K. Mohanty et al., Electrophoresis 2006, 27, 3772,3778]. Batch fabrication time for the device presented here was 1.5,h including channel coating time to suppress electroosmotic flow. Devices were constructed out of poly-isobornyl acrylate and glass. A standard microscope with a UV source was used for sample detection. Separations were demonstrated using Promega BenchTop 100,bp ladder in hydroxyl ethyl cellulose (HEC) and oligonucleotides of 91 and 118,bp were used to characterize sample injection and extraction of DNA bands. The end result was an inexpensive micro-capillary electrophoresis device that uses tools (e.g. micropipette, electrophoretic power supplies, and microscopes) already present in most labs for sample manipulation and detection, making it more accessible for potential end users. [source] Microfluidic technologies for MALDI-MS in proteomicsELECTROPHORESIS, Issue 18 2006Don L. DeVoe Professor Abstract The field of microfluidics continues to offer great promise as an enabling technology for advanced analytical tools. For biomolecular analysis, there is often a critical need to couple on-chip microfluidic sample manipulation with back-end MS. Though interfacing microfluidics to MS has been most often reported through the use of direct ESI-MS, there are compelling reasons for coupling microfluidics to MALDI-MS as an alternative to ESI-MS for both online and offline analysis. The intent of this review is to provide a summary of recent developments in the integration of microfluidic systems with MALDI-MS, with an emphasis on applications in proteomics. Key points are summarized, followed by a review of relevant technologies and a discussion of outlook for the field. [source] Implementation of monoclonal antibody fluorescence on the Abbott CELL-DYN Sapphire haematology analyser: evaluation of lymphoid, myeloid and platelet markersINTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY, Issue 2 2006B. JOHANNESSEN Summary Apart from qualitative flags, that are typically inefficient and uninformative, haematology instruments provide little meaningful information about lymphocyte populations or the lineage of atypical or immature elements, The CELL-DYN Sapphire haematology analyser uses integrated optical and fluorescence (488 nm) measurements, with FL1 (FITC) and FL2 (PE) detectors being configured for fluorescent analysis. As monoclonal antibodies (Mab) are widely used as cellular probes, and are likely to constitute the future basis for immunodifferentials, we explored the feasibility of implementing immunofluorescence on this routine haematology analyser. An extensive series of Mab (CD2, CD3, CD4, CD8, CD11b, CD13, CD14, CD16, CD19, CD22, CD33, CD34, CD41, CD42b, CD45, CD56, CD61, CD64, CD235a and HLA-DR) were tested singly or in FITC/PE combinations. Analyser processing and data acquisition was achieved using CD-Sapphire automated CD61 immunoplatelet or CD3/4/8 assay procedures and, apart from mixing EDTA-blood and antibody, no further sample manipulation was required. Downloaded raw files were processed with cytometry software, and all evaluated reagents showed population discrimination analogous to flow cytometry. Practical procedures were straightforward and required minimal operator training. Extended information that can be obtained from monoclonal antibodies with a routine haematology analyser has the potential to extend haematology laboratory practices and positively impact laboratory and clinical efficiency. [source] Application of solid phase microextraction for the determination of soil fumigants in water and soil samplesJOURNAL OF SEPARATION SCIENCE, JSS, Issue 1 2005Sonia Fuster Abstract The potential of solid phase microextraction (SPME) for the determination of the soil fumigants 1,3-dichloropropene (1,3-DCP) and methyl isothiocyanate (MITC) in environmental samples such as soil and water samples has been investigated. Direct immersion SPME followed by GC/ECD/NPD analysis allowed the rapid determination of the two fumigants in water samples, with very little sample manipulation, giving an LOD of 0.5 ,g L,1. Precision, calculated as relative standard deviation (RSD) for six replicates at three concentration levels, was found to be lower than 20% at the concentration levels tested. For the analysis of soil samples, headspace (HS)-SPME combined with GC/ECD/NPD analysis has been applied. Quantification using matrix-matched calibration curves allowed determination of both analytes (MITC and 1-3-DCP) with a LOD of 0.1 ,g kg,1 (RSD <10%) for the two concentration levels assayed (0.02 and 0.2 mg kg,1). The HS-SPME procedure developed in this paper was applied to soil samples from experimental green house plots treated with metham-Na, a soil disinfestation agent that decomposes in soil to MITC. The absence of sample manipulation as well as the low solvent consumption in SPME methodology are among the main advantages of this analytical approach. [source] First approach of a methodological set-up for selenomethionine chiral speciation in breast and formula milk using high-performance liquid chromatography coupled to atomic fluorescence spectroscopyAPPLIED ORGANOMETALLIC CHEMISTRY, Issue 6 2007J. L. Gómez-Ariza Abstract The chiral speciation of selenomethionine in breast and formula milk based on species separation by high-performance liquid chromatography followed by online microwave-assisted digestion and detection with hydride generation atomic fluorescence spectrometry (HPLC-MAD-HG-AFS) requires severe sample manipulation to avoid matrix influence. Sample clean-up for fat and protein elimination using centrifugation and ultrafiltration was optimized, and selenomethionine preconcentration based on cation exchange solid-phase extraction was studied and optimized. The resulting procedure is suitable for chiral selenium speciation in infant milk with detection limits of 3.1 and 3.5 ng ml,1 as Se for L -selenomethionine and D -selenomethionine, respectively. The time necessary for the analysis, about 90 min, including sample clean-up, analyte preconcentration and chromatographic separation, makes the approach suitable for routine analysis. Copyright © 2007 John Wiley & Sons, Ltd. [source] Identification, control and hysteresis compensation of a 3 DOF metrological AFMASIAN JOURNAL OF CONTROL, Issue 2 2009Roel Merry Abstract Atomic Force Microscopes (AFMs) are widely used for the investigation of samples at the nanometer scale. The metrological AFM used in this work uses a 3 degrees-of-freedom (DOFs) stage, driven by piezo-stack actuators, for sample manipulation in combination with a fixed cantilever. The piezo-stack actuators suffer from hysteresis, which acts as a nonlinear disturbance on the system and/or can change the system dynamics. The contributions of this paper are the application of feedback control to all 3 DOFs of the metrological AFM and the design and application of a hysteresis feedforward for the asymmetric hysteresis present in the system. The amount of coupling between the DOFs is assessed by a non-parametric multiple-input-multiple-output (MIMO) identification. Since the dynamics appear to be decoupled in the frequency range of interest, feedback controllers are designed for each DOF separately. For the modeling of the asymmetric hysteresis an extended Coleman-Hodgdon model is proposed. This model is used for feedforward compensation of the hysteresis. The combination of feedback control for all DOFs and the asymmetric hysteresis feedforward enables the AFM to track scanning profiles within the sensor bound of 5,nm. Real-time imaging of the sample is possible with an accuracy of 2,nm. Copyright © 2009 John Wiley and Sons Asia Pte Ltd and Chinese Automatic Control Society [source] | ||||||||||