Sample Groups (sample + groups)

Distribution by Scientific Domains


Selected Abstracts


The role of specialist and general nurses working with people with multiple sclerosis

JOURNAL OF CLINICAL NURSING, Issue 18 2009
Alison While
Aim., To describe the perceived role of nurses and other carers of people with multiple sclerosis from the perspective of different stakeholders (people with multiple sclerosis, non-specialist nurses, specialist nurses and other health care professionals). Background., Multiple sclerosis is one of the commonest causes of disability in young adults. People with multiple sclerosis require supportive care during the disease trajectory. The role of different health and social care providers has not been reported previously. Design., Survey. Method., Questionnaire data collected in 2002 during the first phase of scale development (health professionals n = 459; people with multiple sclerosis n = 65; total response rate 59·4%). The data were reanalysed to compare responses across sample groups. Results., There was consensus that neurologists and specialist nurses were the most appropriate professionals in the provision of specialist care with specialist nurses also being identified as key providers of emotional support. However, there were also significant differences in nominations reflecting the different perspectives of the stakeholder groups and a self-report bias. Each stakeholder group frequently emphasised their own perceived contribution to care. The different perspective of people with multiple sclerosis was also noteworthy with their greater emphasis on social care and lay support. Conclusions., The findings indicate a dissonance in the views of different stakeholders within the care system. The division of labour associated with nursing care requires further exploration. The contrasting paradigms of health care professionals and people with multiple sclerosis regarding models of disability were highlighted. Relevance to clinical practice., Multiple sclerosis, like other chronic illnesses, requires substantial nursing care. There is a growing number of specialist nurses in the workforce, however, little is known how their role interfaces with other nurses. [source]


Another diet of worms: the applicability of polychaete feeding guilds as a useful conceptual framework and biological variable

MARINE ECOLOGY, Issue 3-4 2005
Paulo Roberto Pagliosa
Abstract A fundamental question in guild studies is how to separate species into guilds. In a seminal manuscript, Fauchald & Jumars [Oceanography and Marine Biology Annual Review17 (1979) 193] summarized polychaete feeding biology and proposed a conceptual framework to test hypotheses on the sympatric occurrence of congeners with limited morphological differentiation. Twenty-six years after this publication, few studies have tested the validity and practical functioning of this scheme of polychaete feeding guilds and then only using part of the classification. The objective of the present study was to analyze the applicability of polychaete feeding guilds to ecological and environment assessments. Two data sets from Santa Catarina Island Bay, southern Brazil, were used. The first data set deals with spatial distribution of natural polychaete assemblages along the bay. The second data set treats fauna in urbanized versus relatively pristine mangroves. Multivariate analysis showed similar patterns in sample groups formed using guilds or densities and composition data. The role of feeding guilds in benthic systems was assessed through comparison with environmental variables. The polychaete assemblage from the Bay was related to sediment type. Motile and discretely motile carnivores and herbivores with jawed probosces matched coarse sands; surface deposit feeders and filter feeders were found in fine sands; and surface and subsurface deposit feeders and carnivores, all with soft probosces matched silt and clay sediments. The data analyses in mangroves showed surface deposit feeders and filter feeders in undisturbed sites and omnivorous species in disturbed ones. The polychaete feeding guilds appear relevant to assembly rules based on resource availability, to resource partitioning and to interspecific competition. [source]


Quantitative analysis of spatial proteoglycan content in articular cartilage with Fourier transform infrared imaging spectroscopy: Critical evaluation of analysis methods and specificity of the parameters

MICROSCOPY RESEARCH AND TECHNIQUE, Issue 5 2010
L. Rieppo
Abstract Objective: To evaluate the specificity of the current Fourier transform infrared imaging spectroscopy (FT-IRIS) methods for the determination of depthwise proteoglycan (PG) content in articular cartilage (AC). In addition, curve fitting was applied to study whether the specificity of FT-IRIS parameters for PG determination could be improved. Methods: Two sample groups from the steer AC were prepared for the study (n = 8 samples/group). In the first group, chondroitinase ABC enzyme was used to degrade the PGs from the superficial cartilage, while the samples in the second group served as the controls. Samples were examined with FT-IRIS and analyzed using previously reported direct absorption spectrum techniques and multivariate methods and, in comparison, by curve fitting. Safranin O-stained sections were measured with digital densitometry to obtain a reference for depthwise PG distribution. Results: Carbohydrate region-based absorption spectrum methods showed a statistically weaker correlation with the PG reference distributions than the results of the curve fitting (subpeak located approximately at 1,060 cm,1). Furthermore, the shape of the depthwise profiles obtained using the curve fitting was more similar to the reference profiles than with the direct absorption spectrum analysis. Conclusions: Results suggest that the current FT-IRIS methods for PG analysis lack the specificity for quantitative measurement of PGs in AC. The curve fitting approach demonstrated that it is possible to improve the specificity of the PG analysis. However, the findings of the present study suggest that further development of the FT-IRIS analysis techniques is still needed. Microsc. Res. Tech. 2010. © 2009 Wiley-Liss, Inc. [source]


Correlation of protein expression, Gleason score and DNA ploidy in prostate cancer

PROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 15 2006
Helena Lexander
Abstract The prognosis of prostate cancer correlates with tumor differentiation. Gleason score and DNA ploidy are two prognostic factors that correlate with prognosis. We analyzed differences in protein expression in prostate cancer of high and low aggressiveness according to these measures. From 35 prostatectomy specimens, 29 cancer samples and 10 benign samples were harvested by scraping cells from cut surfaces. DNA ploidy was assessed by image cytometry. Protein preparations from cell suspensions were examined by 2-DE. Protein spots that differed quantitatively between sample groups were identified by MS fingerprinting of tryptic fragments and MS/MS sequence analysis. We found 39 protein spots with expression levels that were raised or lowered in correlation with Gleason score and/or DNA ploidy pattern (31 overexpressed in high-malignant cancer, 8 underexpressed). Of these, 30 were identified by MS. Among overexpressed proteins were heat-shock, structural and membrane proteins and enzymes involved in gene silencing, protein synthesis/degradation, mitochondrial protein import (metaxin 2), detoxification (GST-pi) and energy metabolism. Stroma-associated proteins were generally underexpressed. The protein expression of prostate cancer correlates with tumor differentiation. Potential prognostic markers may be found among proteins that are differentially expressed and the clinical value of these should be validated. [source]


SAR and efficiency evaluation of a 900 MHz waveguide chamber for cell exposure

BIOELECTROMAGNETICS, Issue 6 2008
Giuseppe De Prisco
Abstract In this work we present the results of numerical and experimental dosimetry carried out for an in vitro exposure device to irradiate sample groups at 900 MHz. The cells are kept in 8 and 15 ml cell cultures, contained, respectively in T25 and T75 rectangular flasks. The dosimetric assessment of the distribution of the specific absorption rate (SAR) is performed for both the bottom of the flask and the whole volume of the sample to provide results for experiments on either the cell layer or the cell suspension. The irradiating chamber is a rectangular waveguide (WG). Different configurations are considered to assess the optimum orientation and positioning of the cell cultures inside the WG. The system performance is optimal when the electric field is parallel to the sample and the WG is terminated by a matched load. In this condition two 15 or four 8 ml cells cultures can be exposed. The efficiency (ratio between the power absorbed by the sample and the incident power) and the non-uniformity degree (ratio between the standard deviation of SAR values and the average SAR over the sample) are calculated and successfully verified through measurements of the scattering parameters and local temperature increases. In the chosen exposure configuration, the efficiency is 0.40 and the non-uniformity degree is 39% for the 15 ml samples. For the 8 ml samples, the efficiency is 0.19 and a low non-uniformity degree (15%) is found. Bioelectromagnetics 29:429,438, 2008. © 2008 Wiley-Liss, Inc. [source]


Phylogeography and genetic structure of the edible sea urchin Paracentrotus lividus (Echinodermata: Echinoidea) inferred from the mitochondrial cytochrome b gene

BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, Issue 4 2010
FERRUCCIO MALTAGLIATI
Phylogeographical analysis of Paracentrotus lividus was carried out by means of sequencing the mitochondrial cytochrome b gene (1143 bp) of 260 individuals collected at 22 Mediterranean and four Atlantic localities. Against a background of high haplotype diversity and shallow genetic structuring, we observed significant genetic divergence between the Adriatic Sea and the rest of the Mediterranean, as well as between the Mediterranean and the Atlantic sample groups. Furthermore, on the largest spatial scale, isolation by distance was detected. Three main haplogroups were identified by network and Bayesian assignment analyses. The relative proportions of haplogroups were different in the four regions considered, with the exception of Western and Eastern Mediterranean that showed a similar pattern. This result together with the outcome of Snn statistics, analysis of molecular variance and network analyses allowed to identify three weakly differentiated populations corresponding to the Atlantic, Western + Eastern Mediterranean, and Adriatic seas. Analyses of mismatch distribution and neutrality tests were consistent with the presence of genetic structuring and past demographic expansion(s). From a fisheries perspective, the results obtained in the present study are consistent with genetic sustainability of current exploitation; local depleted stocks are recurrently replenished by recruits that may have originated from nonharvested areas. © 2010 The Linnean Society of London, Biological Journal of the Linnean Society, 2010, 100, 910,923. [source]