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Resolution Power (resolution + power)
Selected AbstractsCE combined with rolling circle amplification for sensitive DNA detectionELECTROPHORESIS, Issue 2 2008Ni Li Abstract Here we describe an assay which combines CE with rolling circle amplification (RCA) for sensitive DNA detection and quantification. RCA is an isothermal DNA replication technique that generates a long ssDNA with tandem repeats. It requires simpler temperature control in reaction and offers higher sequence specificity and greater quantitation capability compared to other amplification technologies. In this study, RCA amplified the DNA target via a circular template, and the product was digested into monomers for CE analysis. Less than 2,fmol of the DNA target could easily be detected using this RCA-CE assay and the assay has a dynamic range of two orders of magnitudes. Moreover, simultaneous detection of both the target DNA and the internal standard was achieved by designing two padlock probes with different sizes, which could significantly improve the quantification accuracy. The RCA-CE assay is easy to perform, readily adaptable for detection of multiple targets because of the high resolution power of CE, and is compatible with other applications employing RCA as a signal amplification tool. Additionally, this assay can be used with a capillary array system to perform sensitive, high-throughput genetic screening. [source] Reverse modelling for seismic event characterizationGEOPHYSICAL JOURNAL INTERNATIONAL, Issue 1 2005Dirk Gajewski SUMMARY The localization of seismic events is of utmost importance in seismology and exploration. Current techniques rely on the fact that the recorded event is detectable at most of the stations of a seismic network. Weak events, not visible in the individual seismogram of the network, are missed out. We present an approach, where no picking of events in the seismograms of the recording network is required. The observed wavefield of the network is reversed in time and then considered as the boundary value for the reverse modelling. Assuming the correct velocity model, the reversely modelled wavefield focuses on the hypocentre of the seismic event. The origin time of the event is given by the time where maximum focussing is observed. The spatial extent of the focus resembles the resolution power of the recorded wavefield and the acquisition. This automatically provides the uncertainty in the localization with respect to the bandwidth of the recorded data. The method is particularly useful for the upcoming large passive networks since no picking is required. It has great potential for localizing very weak events, not detectable in the individual seismogram, since the reverse modelling sums the energy of all recorded traces and, therefore, enhances the signal-to-noise ratio similar to stacking in seismic exploration. The method is demonstrated by 2-D and 3-D numerical case studies, which show the potential of the technique. Events with a S/N ratio smaller than 1 where the events cannot be identified in the individual seismogram of the network are localized very well by the method. [source] Structure determination without Fourier inversion.ACTA CRYSTALLOGRAPHICA SECTION A, Issue 6 2009The parameter-space concept for solving crystal structures from reflection amplitudes (without employing or searching for their phases) is described on a theoretically oriented basis. Emphasis is placed on the principles of the method, on selecting one of three types of parameter spaces discussed in this paper, and in particular on the structure model employed (equal-atom point model, however usually reduced to one-dimensional projections) and on the system of `isosurfaces' representing experimental `geometrical structure amplitudes' in an orthonormal parameter space of as many dimensions as unknown atomic coordinates. The symmetry of the parameter space as well as of the imprinted isosurfaces and its effect on solution methods is discussed. For point atoms scattering with different phases or signs (as is possible in the case of X-ray resonant or of neutron scattering) it is demonstrated that the `landscape' of these isosurfaces remains invariant save certain shifts of origin known beforehand (under the condition that all atomic scattering amplitudes have been reduced to 1 thus meeting the requirement of the structure model above). Partly referring to earlier publications on the subject, measures are briefly described which permit circumventing an analytical solution of the system of structure-amplitude equations and lead to either a unique (unequivocal) approximate structure solution (offering rather high spatial resolution) or to all possible solutions permitted by the experimental data used (thus including also all potential `false minima'). A simple connection to Patterson vectors is given, also a first hint on data errors. References are given for practical details of various solution techniques already tested and for reconstruction of three-dimensional structures from their projections by `point tomography'. We would feel foolish if we tried to aim at any kind of `competition' to existing methods. Having mentioned `pros and cons' of our concept, some ideas about potential applications are nevertheless offered which are mainly based on its inherent resolution power though demanding rather few reflection data (use of optimal intensity contrast included) and possibly providing a result proven to be unique. [source] The concordance test emerges as a powerful tool for identifying quantitative trait nucleotides: lessons from BTA6 milk yield QTLANIMAL GENETICS, Issue 2 2009E. Seroussi Summary The lack of conventions for confirming the discovery of quantitative trait nucleotides in livestock was evidenced by the proposals of mutations in two different genes (SPP1 and ABCG2) as the underlying functional mutation for a major quantitative trait locus (QTL) for milk concentration on bovine chromosome 6 (BTA6). Of these conflicting candidates, SPP1 was excluded by follow-up studies and by the data described here. A simple test for concordance of the zygosity state between QTL segregation status and the candidate polymorphism was shown, in this case, to be a critical step towards establishing the proof. If a given sample effectively represents the genetic variation across the QTL region, haplotype-based concordance may further enhance the functionality and resolution power of this test, allowing identification of the causative gene. [source] Biomedical applications of capillary electrophoresis with laser-induced fluorescence detectionBIOPHARMACEUTICS AND DRUG DISPOSITION, Issue 7-8 2001Ximena Páez Abstract Capillary electrophoresis (CE) is a high-efficiency analytical technique that has had a great impact as a tool in biomedical research, clinical and forensic practice in the last ten years. Only in one of the applications, the DNA analysis, it has had an explosive exponential growth in the last few years. This impact is expressed in an enormous amount of CE articles and many reviews. The CE advantages with respect to other analytical techniques: the required very small sample volume, rapid analysis, great resolution power and low costs, have made this technique ideal for the analysis of a numerous endogenous and exogenous substances present in biological fluids. The different modes of CE have been coupled to different detection techniques such as UV-absorbance, electrochemical, mass spectrometry and laser-induced fluorescence detection (LIFD) to detect different nature and molecular size separated analytes. This review focuses mostly on the applications of CE,LIFD, to measure drugs and endogenous neuroactive substances such as amino acids and monoamines, especially in microdialysis samples from experimental animals and humans. CE,LIFD trends are discussed: automated faster analysis with capillary array systems, resolution power improvement, higher detection sensitivity, and CE systems miniaturization for extremely small sample volume, in order to make CE easier and affordable to the lab bench or the clinical bed. Copyright © 2001 John Wiley & Sons, Ltd. [source] Investigation of a tartaric acid-based linear polyamide and dimer as chiral selectors in liquid chromatographyCHIRALITY, Issue 2 2005Joakim Oxelbark Abstract A twin selector for enantioselective liquid chromatography based on O,O,-bis(dimethyl)benzoyl tartaric diamide was synthesized and compared to commercially available Kromasil CHI-DMB (O,O,-bis(dimethyl)benzoyl tartaric diamide). A linear polyamide based on the same tartaric acid derivative was also synthesized, immobilized on silica, and evaluated as stationary phase. The twin selector was immobilized as a brush-type phase, with similar bonding chemistry as in Kromasil CHI-DMB. It was shown to exhibit lower resolution power than Kromasil CHI-DMB. However, retention and separation factors obtained on the respective sorbents were shown to exhibit interdependence. CD spectra of the twin selector give no indication that the respective branches interact in the solvent mixtures employed for chromatography. The linear polyamide showed lower enantioselectivity and higher retention than Kromasil CHI-DMB. Chirality 17:79,84, 2005. © 2005 Wiley-Liss, Inc. [source] | |||||||||||||