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Regeneration Potential (regeneration + potential)

Distribution by Scientific Domains

Medical Sciences	42%
Life Sciences	42%

Selected Abstracts

Regeneration potential of CIMMYT durum wheat and triticale varieties from immature embryos

PLANT BREEDING, Issue 4 2001
N. E. Bohorova
Abstract Twenty-five durum wheat elite advanced lines and released varieties, and five triticale varieties were evaluated for their ability to produce embryogenic callus using three different media. For callus initiation and maintenance there were basal Murashige and Skoog (MS) medium containing double strains of macroelements and 2.5 mg/l 2,4D (DW1), basal MS medium containing 2.0 mg/l 2,4D (DW2), or basal MS medium supplemented with 1.0 mg/l 2,4 D and coconut milk (DW3). Plant regeneration was achieved on basal MS medium with indoleacetic acid and 6-benzylaminopurine, and plants rooted on MS with 1-naphthale-neacetic acid. DW3 medium proved better than the other media tested for embryogenic callus initiation and maintenance. Regeneration rates varied widely with both genotype and initiation medium, with values ranging from no regeneration to 100% regeneration; the plantlets produced per embryo ranged from five to 20. Fourteen of the durum wheat genotypes showed 63,100% regeneration from DW3 callus formation medium, four lines from DW1 medium, and two lines from DW2. Four of the triticale varieties had regeneration of 48,100% from DW3 medium. After six subcultures, over a 6-month period, genotypes lost their ability to regenerate plants. Only 10 lines retained some plant regeneration potential but regeneration was at reduced levels. Successful regeneration of durum wheat and triticale varieties will be used as an integral part of the transformation process. [source]

Repair of segmental defects in rabbit humeri with titanium fiber mesh cylinders containing recombinant human bone morphogenetic protein-2 (rhBMP-2) and a synthetic polymer

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, Issue 2 2002
Narumichi Murakami
Abstract To develop a new technology that enhances the regeneration potential of bone and the repair of large intercalated defects in long bone, recombinant human bone morphogenetic protein-2 (BMP-2; 20 ,g or 40 ,g) was mixed in a polymer gel (poly-lactic acid-polyethyleneglycol block copolymer; PLA-PEG; 200 mg) and incorporated into titanium fiber-mesh cylinders. Three 5-mm cylinders were placed end-to-end to fill a 15-mm defect created in the humeri of adult rabbits and were stabilized by an intramedullary rod. In controls, the titanium fiber-mesh cylinders were combined with PLA-PEG in the absence of BMP. Six weeks after implantation, new bone had formed on the surface of the implant and had bridged the defect. All of the defects (5/5) treated by cylinders containing 120 ,g (40 ,g × 3) of BMP were repaired completely. New bone formation was also found inside the pores of the cylinders. The defect was not repaired in the control animals. These results demonstrate that these new composite implants fabricated by combining rhBMP, synthetic degradable polymers and compatible biomaterials enhance the regeneration potential of bone. Thus, it is possible that large skeletal defects can be repaired using this prosthesis in lieu of autogenous bone graft. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 62: 169,174, 2002 [source]

Secretion of SDF-1, by bone marrow-derived stromal cells enhances skin wound healing of C57BL/6 mice exposed to ionizing radiation

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, Issue 6b 2010
Yannick Landry
Abstract Patients treated for cancer therapy using ionizing radiation (IR) have delayed tissue repair and regeneration. The mechanisms mediating these defects remain largely unknown at present, thus limiting the development of therapeutic approaches. Using a wound healing model, we here investigate the mechanisms by which IR exposure limits skin regeneration. Our data show that induction of the stromal cell-derived growth factor 1, (SDF-1,) is severely impaired in the wounded skin of irradiated, compared to non-irradiated, mice. Hence, we evaluated the potential of bone marrow-derived multipotent stromal cells (MSCs), which secrete high levels of SDF-1,, to improve skin regeneration in irradiated mice. Injection of MSCs into the wound margin led to remarkable enhancement of skin healing in mice exposed to IR. Injection of irradiated MSCs into the wound periphery of non-irradiated mice delayed wound closure, also suggesting an important role for the stromal microenvironment in skin repair. The beneficial actions of MSCs were mainly paracrine, as the cells did not differentiate into keratinocytes. Specific knockdown of SDF-1, expression led to drastically reduced efficiency of MSCs in improving wound closure, indicating that SDF-1, secretion by MSCs is largely responsible for their beneficial action. We also found that one mechanism by which SDF-1, enhances wound closure likely involves increased skin vascularization. Our findings collectively indicate that SDF-1, is an important deregulated cytokine in irradiated wounded skin, and that the decline in tissue regeneration potential following IR can be reversed, given adequate microenvironmental support [source]

Status of the Mara Woodlands in Kenya

AFRICAN JOURNAL OF ECOLOGY, Issue 3 2004
Matthew J. Walpole
Abstract The woodlands of the Masai Mara National Reserve in Kenya have suffered dramatic declines over four decades as a result of elephant and fire pressure. This study examined the current status of woody resources in the Reserve and browse pressure thereon, using both classification (TWINSPAN) and ordination (DCA) techniques. From 333 widespread regular plots used to survey the vegetation, a total of 62 woody species were identified. Thirteen woody habitats were identified on the basis of species composition, varying from species-rich closed thickets and forest to less diverse open grasslands. A NW/SE stratification of the more open habitats was observed, possibly as a result of differences in soils, rainfall and drainage. Both plant density and diversity were lower than in communal and privately managed areas outside the Reserve. Moreover, browser pressure was substantially higher than that observed previously in the ecosystem, and suggests increased competition for scarcer woody resources within the Reserve. This has implications for the management of the ecosystem as a whole. As woodland and thickets continue to decline, long-term monitoring should expand to encompass the wider habitat diversity of the open grasslands and unprotected areas where much of the regeneration potential resides. Résumé Les forêts de la Réserve Nationale de Masai Mara, au Kenya, ont subi des réductions dramatiques depuis quatre décennies, suite à la pression des éléphants et des feux. Cette étude a examiné le statut actuel des ressources en bois dans la Réserve et la pression du pâturage qui s'y ajoute, en utilisant les techniques de classification (TWINSPAN) et d'ordination (DCA). Dans 333 plots régulièrement dispersés, utilisés pour étudier la végétation, on a identifié 62 espèces ligneuses. On a identifié aussi 13 habitats forestiers sur la base de la composition des espèces, depuis les buissons et les forêts fermés riches en espèces jusqu'aux prairies ouvertes moins diverses. On a observé une stratification NO/SE dans les habitats plus ouverts, résultat peut-être de différences de sols, de chutes de pluies et de drainage. La densité et la diversité des plantes étaient plus faibles que dans les aires gérées en commun ou de façon privée en dehors de la Réserve. Qui plus est, la pression des animaux qui mangent les buissons y était substantiellement plus élevée que celle qu'on observait jadis dans cet écosystème, et elle laisse penser qu'il y a une compétition plus forte pour des ressources ligneuses plus rares dans la Réserve. Ceci a des implications pour la gestion de l'écosystème dans son ensemble. Si les forêts et les buissons continuent à se rèduire, le monitoring à long terme devrait s'élargir pour englober la plus grande diversité d,habitat des prairies ouvertes et des aires non protégées où réside l'essentiel du potentiel de régénération. [source]

Regeneration potential of CIMMYT durum wheat and triticale varieties from immature embryos

The influence of sex on the chondrogenic potential of muscle-derived stem cells: Implications for cartilage regeneration and repair

ARTHRITIS & RHEUMATISM, Issue 12 2008
Tomoyuki Matsumoto
Objective To explore possible differences in muscle-derived stem cell (MDSC) chondrogenic differentiation in vitro and articular cartilage regeneration in vivo between murine male MDSCs (M-MDSCs) and female MDSCs (F-MDSCs). Methods Three different populations of M- and F-MDSCs (n = 3 of each sex) obtained via preplate technique, which separates cells based on their variable adhesion characteristics, were compared for their in vitro chondrogenic potential using pellet culture. Cells were assayed with and without retroviral transduction to express bone morphogenetic protein 4 (BMP-4). The influence of both expression of stem cell marker Sca1 and in vitro expansion on the chondrogenic potential of M- and F-MDSCs was also determined. Additionally, BMP-4,transduced M- and F-MDSCs were applied to a full-thickness articular cartilage defect (n = 5 each) on the femur of a nude rat, and the quality of the repaired tissue was evaluated by macroscopic and histologic examination. Results With and without BMP-4 gene transduction, M-MDSCs produced significantly larger pellets with a richer extracellular matrix, compared with F-MDSCs. Sca1 purification influenced the chondrogenic potential of MDSCs, especially M-MDSCs. Long-term culture did not affect the chondrogenic potential of M-MDSCs but did influence F-MDSCs. M-MDSCs repaired articular cartilage defects more effectively than did F-MDSCs at all time points tested, as assessed both macroscopically and histologically. Conclusion Our findings demonstrate that sex influences the chondrogenic differentiation and articular cartilage regeneration potential of MDSCs. Compared with female MDSCs, male MDSCs display more chondrogenic differentiation and better cartilage regeneration potential. [source]

A Novel Approach to Align Adult Neural Stem Cells on Micropatterned Conduits for Peripheral Nerve Regeneration: A Feasibility Study

ARTIFICIAL ORGANS, Issue 1 2009
Shan-hui Hsu
Abstract There is a strong need for nerve-tissue engineering using the guide conduit and Schwann cells or neural stem cells (NSCs) with regeneration potential for injured peripheral nerves. In this study, micropatterned poly(d,l -lactide) (PLA) conduits were fabricated by microlithography and solvent-casting. The PLA conduits were seeded with the novel green fluorescent protein (GFP)-positive adult mouse NSCs obtained using the patented method of one of the authors. About 85% of the seeded NSCs were successfully aligned on the micropatterned conduits within 72 h and expressed the genes related to the production of neurotrophic factors. Gene expressions for the neurotrophic factors, such as nerve growth factor and brain-derived neurotrophic factor were upregulated by the micropatterned conduits at 72 h. The micropatterned PLA conduits seeded with the aligned NSCs were used to bridge the 10-mm sciatic nerve gaps in rats and were found to facilitate nerve repair and functional recovery during a period of 6 weeks compared with the nonseeded group. This model can be used to study the role of adult NSCs in peripheral-nerve regeneration in the future. [source]