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Repellent
Kinds of Repellent Terms modified by Repellent Selected AbstractsBoards of Directors and Shark Repellents:Assessing the Value of an Agency Theory PerspectiveJOURNAL OF MANAGEMENT STUDIES, Issue 3 2000Steven A. Frankforter Because shark repellents decrease the vulnerability of firms (and their incumbent managers) to the market for corporate control, the decision to adopt these devices represents an excellent test of agency theory. In this empirical study, we examined the relationships between the adoption of shark repellents and several mechanisms that, according to agency theory, should align the interests of corporate board members and shareholders and/or make directors more effective monitors of management behaviour. Of the variables included, only board stock ownership (especially by employee directors) was linked to a reduced propensity to adopt shark repellents in the predicted manner. Two variables not immediately as- sociated with agency theory , the proportion of inside directors appointed by the incumbent chief executive officer (CEO) and a lower ratio of CEO compensation to the compensation of other top executives , were linked to higher rates of shark repellent adoption. Given that agency theory explains relatively little of the variance in shark repellent adoption, we advocate serious consideration of other theoretical formulations for corporate governance, including two approaches , stewardship theory and agent morality , that take the moral (,other regarding') obligations of directors seriously. [source] Application of Insect Repellents by Travelers to Malaria Endemic AreasJOURNAL OF TRAVEL MEDICINE, Issue 4 2006Yvonne Thrower MSc Background The use of insect repellents applied to the skin is always encouraged in those visiting malaria endemic areas. There are a number of factors contributing to the efficacy of insect repellents in the field and an important factor contributing to their longevity relates to the applied dose. This study investigated the dose of insect repellent that travelers might normally apply to the skin. Methods Subjects were asked to apply a product they had purchased to the arms and neck, and the dose applied (mg/cm2) was calculated. The study was conducted using three different populations; 74 travelers to malaria endemic areas visiting pharmacies to purchase repellents before they left the UK, 23 travelers on safari in India, and 24 on safari in Kenya. Results There was a significant difference in the mean applied arm dose of product (mg/cm2) [p < 0.001, analysis of variance (ANOVA)]; UK 1.15 (SD 0.29), India 0.96 (SD 0.23), and Kenya 1.34 (SD 0.54). Higher concentrations were achieved on the neck area. Few of the participants in the field trial used long-sleeved shirts at night. Even using 50% wt/vol repellent products, 32% in the Kenya and 25% in the India, study achieved a dose of active ingredient less than 0.5 mg/cm2. Conclusion These pilot studies would indicate that travelers tend to apply below the optimal concentration of active ingredient of repellent as might be suggested by mathematical models. [source] An ecological cost of plant defence: attractiveness of bitter cucumber plants to natural enemies of herbivoresECOLOGY LETTERS, Issue 3 2002Anurag A. Agrawal Abstract Plants produce defences that act directly on herbivores and indirectly via the attraction of natural enemies of herbivores. We examined the pleiotropic effects of direct chemical defence production on indirect defence employing near-isogenic varieties of cucumber plants (Cucumis sativus) that differ qualitatively in the production of terpenoid cucurbitacins, the most bitter compounds known. In release,recapture experiments conducted in greenhouse common gardens, blind predatory mites were attracted to plants infested by herbivorous mites. Infested sweet plants (lacking cucurbitacins), however, attracted 37% more predatory mites than infested bitter plants (that produce constitutive and inducible cucurbitacins). Analysis of the headspace of plants revealed that production of cucurbitacins was genetically correlated with large increases in the qualitative and quantitative spectrum of volatile compounds produced by plants, including induced production of (E,)-,-ocimene (3E,)-4,8-dimethyl-1,3,7-nonatriene, (E,E)-,-farnesene, and methyl salicylate, all known to be attractants of predators. Nevertheless, plants that produced cucurbitacins were less attractive to predatory mites than plants that lacked cucurbitacins and predators were also half as fecund on these bitter plants. Thus, we provide novel evidence for an ecological trade-off between direct and indirect plant defence. This cost of defence is mediated by the effects of cucurbitacins on predator fecundity and potentially by the production of volatile compounds that may be repellent to predators. [source] Percolation characteristics of a water-repellent sandy forest soilEUROPEAN JOURNAL OF SOIL SCIENCE, Issue 1 2008G. Wessolek Summary In a tracer experiment TDR transect measurements were made to study percolation behaviour in a 120-year-old pine stand (Pinus sylvestris) on a water-repellent sandy soil (Haplic Arenosol). The experiment (with potassium iodide) showed an 80% labelling of the total flow in organic layers, whereas the area of transport in the mineral soil was sharply reduced to 12,30%. The average diameters of these preferential flow paths were about 8,15 cm. The TDR measurements indicate a homogeneous flow only for a short period from February until April. At this time of the year preferential flow is insignificant, because the soil is at approximately field capacity and not repellent to water. During summer (May to September) the soil dries out, and most precipitation results in preferential flow during this period. For any daily rainfall exceeding 10 mm, water infiltrates down to 1 m depth in the soil, which nevertheless, is still within the root zone. This kind of deep percolation results in the subsoil's wetting to field capacity (pF 1.8) earlier than the topsoil. A one-dimensional numerical model (SWAP) was used to simulate mean water balance with hydraulic functions with and without a water-repellency term. From the results of our tracer experiment we showed that the de-watering process in spring could be simulated well using the traditional piston flow concept, while the rewetting behaviour could be described more realistically using the mobile,immobile concept for water repellency. [source] WARNING DISPLAYS IN SPINY ANIMALS: ONE (MORE) EVOLUTIONARY ROUTE TO APOSEMATISMEVOLUTION, Issue 12 2005Michael P. Speed Abstract To date, theoretical or laboratory simulations of aposematic evolution in prey animals have focused narrowly on internally stored chemical defense as the source of unprofitability and ignore aposematic advertisement of physical defenses such as spines (and defensive hairs, claws, etc.). This has occurred even though aposematism in spiny animals has been recognized since the 19th century. In this paper we present the first detailed theoretical consideration of aposematism in spiny animals, focusing on questions of initial evolution, costs of display, and coevolution of displays with defenses. Using an individual-based evolutionary model, we found that spines (or similar physical defenses) can easily evolve without aposematism, but when spines do evolve, aposematic displays can also easily evolve if they help to make the prey animal distinctive and if they draw attention to the physical threat. When aposematic displays evolve, they cause reduced investment in costly spines, so that, in addition to signaling unprofitability, aposematic display may enhance the cost-effectiveness of antipredator defenses (one exception to this conclusion is if the display is itself as costly as the defense). For animals with stinging spines, combining physical and chemical defense, the evolution of aposematic display may lead to reduced investment in the toxin compared to the spine. This occurs because spines act as both secondary (repellent) defenses and as primary defenses (their own visible, honest advertisement), whereas internally stored toxins only (generally) act as repellent secondary defenses. We argue that conspicuous aposematism in spines functions as an attention-getting mechanism, whereas conspicuous aposematic display in purely toxic animals may be explained by signal reliability arguments. Finally, one (more) route by which aposematism may initially evolve is by spiny rather than purely chemically defended species, spreading to species with other forms of secondary defense as the signal becomes common. [source] Corymbia (= Eucalyptus) maculata citriodon effective as insect repellentFOCUS ON ALTERNATIVE AND COMPLEMENTARY THERAPIES AN EVIDENCE-BASED APPROACH, Issue 2 2008Article first published online: 3 JUN 2010 [source] Efficacy of four insect repellents against mosquito bites: a double-blind randomized placebo-controlled field study in SenegalFUNDAMENTAL & CLINICAL PHARMACOLOGY, Issue 5 2009Bernard Uzzan Abstract Insect-borne diseases represent a worldwide threat. In addition to fight against vectors (insecticides) and disease prevention (vaccination against yellow fever, chemoprophylaxis against malaria), insect repellents applied on the skin could help reduce the heavy burden related to these diseases. In a field study performed in Senegal, we compared the efficacy of one skin application between 3 and 4 p.m. of four spray repellents [icaridine 20%, para-menthane-diol (PMD) 20% and 50% and DEET 50%] against placebo, among 100 healthy male and female volunteers experienced with mosquito capture. Double-blind randomized cross-over placebo-controlled study (Latin-square design) during five consecutive nights (7 p.m. to midnight) in two villages was conducted. To avoid residual effect, right or left leg was alternately exposed during consecutive nights and the exposed leg was washed before next night. The statistical model was random and mixed effects anova. All four active repellents provided a significant and similar protection compared with placebo, lasting 8 h. However, there was a non-significant trend for a higher protection by DEET 50% than by PMD 20% (P = 0.07). Duration of protection was similar for all repellents. Their effects were similar among men and women, and against Anopheles or other species. No serious adverse drug reaction was noticed. Using a rigorous methodology and a large number of volunteers, our well-controlled study demonstrated an important and similar protective effect of all four repellents compared with placebo. Such field studies should be required before approval of any newly developed repellent. [source] Pharmaceuticals and Other Organic Waste Water Contaminants Within a Leachate Plume Downgradient of a Municipal LandfillGROUND WATER MONITORING & REMEDIATION, Issue 2 2004Kimberlee K. Barnes Ground water samples collected from the Norman Landfill research site in central Oklahoma were analyzed as part of the U.S. Geological Survey (USGS) Toxic Substances Hydrology Program's national reconnaissance of pharmaceuticals and other organic waste water contaminants (OWCs) in ground water. Five sites, four of which are located downgradient of the landfill, were sampled in 2000 and analyzed for 76 OWCs using four research methods developed by the USGS. OWCs were detected in water samples from all of the sites sampled, with 22 of the 76 OWCs being detected at least once. Cholesterol (a plant and animal steroid), was detected at all five sites and was the only compound detected in a well upgradient of the landfill. N,N-diethyltoluamide (DEBT used in insect repellent) and tri(2-chloroethyl) phosphate (fire-retardant) were detected in water samples from all four sites located within the landfill-derived leachate plume. The sites closest to the landfill had more detections and greater concentrations of each of the detected compounds than sites located farther away. Detection of multiple OWCs occurred in the four sites located within the leachate plume, with a minimum of four and a maximum of 17 OWCs detected. Because the landfill was established in the 1920s and closed in 1985, many compounds detected in the leachate plume were likely disposed of decades ago. These results indicate the potential for long-term persistence and transport of some OWCs in ground water. [source] Quantifying the impact of soil water repellency on overland flow generation and erosion: a new approach using rainfall simulation and wetting agent on in situ soilHYDROLOGICAL PROCESSES, Issue 17 2007G. Leighton-Boyce Abstract The conventional view of soil water repellency is that it promotes overland flow and soil erosion, but this is not always borne out by observations. This study aimed to isolate the effects of repellency on long-unburnt and recently burnt terrain on infiltration, overland flow and erosion at the small plot scale (0·36 m2). Rainfall simulations (30 min; intensity 100 mm h,1), using untreated water, and water treated with surfactants to eliminate repellency, were conducted on in situ repellent soils in fire-prone Eucalyptus globulus plantations, north-central Portugal at (i) a long-unburnt site with and without litter, and (ii) a recently burnt site. On long-unburnt terrain, the mean overland flow coefficient (33%) was 16 times higher and mean slopewash was 23 times higher under repellent compared with wettable conditions. On recently burnt terrain, no overland flow was recorded under wettable conditions, while under repellent conditions the mean coefficient was 70%. The water storage capacity of the litter layer under 10-year-old eucalyptus stands for dry antecedent conditions was at least 3 mm water depth per cm litter depth, implying at least a delay to the onset of overland flow. Severe repellency (36% ethanol) was found to persist through a 30-min storm (100 mm h,1) when a litter layer was present. A continuous wetting front was observed in the upper ,1 cm of exposed soil, indicating a breakdown in repellency at the time of observation. Below ,1 cm, repellent, dry soil conditions generally persisted through the simulated storm event. A major implication is that prediction of hydrological impacts of repellency must also take into account the infiltration characteristics of any litter layer and any non-repellent soils, if present. Copyright © 2007 John Wiley & Sons, Ltd. [source] Infiltration into effluent irrigation-induced repellent soils and the dependence of repellency on ambient relative humidityHYDROLOGICAL PROCESSES, Issue 17 2007R. Wallach Abstract As a result of water scarcity and as a means of wastewater disposal, reuse of treated sewage effluent in irrigated agriculture is practiced worldwide. Among the detrimental aspects of wastewater re-use in agriculture is the possibility that soils will be rendered water repellent. The current study focuses on time dependent variation of infiltration rate in effluent-induced repellent soils, and time dependent variation in water repellency at different levels of ambient relative humidity (RH). The shape of the cumulative infiltration curve of water was found to depend on the repellency degree (concave for wettable and slightly repellent soils, convex for severely repellent soil). Compared with infiltration rates in the wettable and slightly repellent soils, infiltration rates in the severely repellent soil were very low at the beginning and then increased. When the liquid-vapor surface tension was reduced by means of ethanol addition to the infiltrating solution, the cumulative infiltration curve of the severely repellent soil also became concave. Repellency degree (as measured by WDPT) was found to be essentially constant over a large range of ambient RH values (<10 , ,81%), and to increase sharply at values above 90%. The relative increase in water drop penetration time (WDPT) at high RH was greatest for the least repellent soil (10-fold increase in WDPT), and least for the most repellent soil (2-fold increase in WDPT). At RH > 90%, the time to reach equilibrium with respect to WDPT and soil moisture content was similar. In contrast, at values of ambient RH ranging from < 10 to 81%, WDPT was invariant over the course of reaching equilibrium with respect to moisture content. However, after reaching moisture content equilibrium, WDPT declined with increasing time. Copyright © 2007 John Wiley & Sons, Ltd. [source] Soil water repellency in a Japanese cypress plantation restricts increases in soil water storage during rainfall eventsHYDROLOGICAL PROCESSES, Issue 17 2007Masahiro Kobayashi Abstract Forest soils in Japan are often water repellent. Substantial water repellency frequently occurs and impedes water infiltration into the soil matrix, but continuous overland flow is not necessarily observed because forest soils usually have macropores through which the water can enter the subsoil. Although this flow pattern may influence the manner of water storage in forest soils at the solum scale, field evidence has not yet indicated this process. We monitored soil water storage during natural rainfall events in a 60-cm deep solum using time domain reflectometry (TDR) moisture sensors, and observed stained flow patterns in the soil following simulated rainfall containing a colour dye, on a slope planted with Japanese cypress (Chamaecyparis obtusa). The surface soil at the research plot exhibited strong water repellency at water contents lower than the threshold critical water content of 0·29 m3 m,3. Under dry antecedent moisture conditions, increases in soil water storage were small compared to the cumulative rainfall, despite the low wetness of the soil matrix. In contrast, under moderate moisture conditions, increases in the water content corresponded to the cumulative rainfall. Under dry conditions, rainwater may have entered the subsoil at a few limited locations connected with continuous vertical macropores, such as decayed root channels or interstructural voids. Therefore, the water seemed to bypass a large part of the soil matrix away from the macropores. Such preferential water flow was confirmed by the stained flow patterns after the rainfall simulation. The flow patterns visualized by the dye were discontinuous and scattered under dry conditions and diffuse under moderate moisture conditions. Repellency induced preferential flow led to restricted increases in solum scale water storage during rainfall events, reflecting a physical nonequilibrium in soil water storage. Copyright © 2007 John Wiley & Sons, Ltd. [source] Assessing burn severity and comparing soil water repellency, Hayman Fire, ColoradoHYDROLOGICAL PROCESSES, Issue 1 2006Sarah A. Lewis Abstract An important element of evaluating a large wildfire is to assess its effects on the soil in order to predict the potential watershed response. After the 55 000 ha Hayman Fire on the Colorado Front Range, 24 soil and vegetation variables were measured to determine the key variables that could be used for a rapid field assessment of burn severity. The percentage of exposed mineral soil and litter cover proved to be the best predictors of burn severity in this environment. Two burn severity classifications, one from a statistical classification tree and the other a Burned Area Emergency Response (BAER) burn severity map, were compared with measured ,ground truth' burn severity at 183 plots and were 56% and 69% accurate, respectively. This study also compared water repellency measurements made with the water drop penetration time (WDPT) test and a mini-disk infiltrometer (MDI) test. At the soil surface, the moderate and highly burned sites had the strongest water repellency, yet were not significantly different from each other. Areas burned at moderate severity had 1·5 times more plots that were strongly water repellent at the surface than the areas burned at high severity. However, the high severity plots most likely had a deeper water repellent layer that was not detected with our surface tests. The WDPT and MDI values had an overall correlation of r = ,0·64(p < 0·0001) and appeared to be compatible methods for assessing soil water repellency in the field. Both tests represent point measurements of a soil characteristic that has large spatial variability; hence, results from both tests reflect that variability, accounting for much of the remaining variance. The MDI is easier to use, takes about 1 min to assess a strongly water repellent soil and provides two indicators of water repellency: the time to start of infiltration and a relative infiltration rate. Copyright © 2005 John Wiley & Sons, Ltd. [source] Causes and consequences of fire-induced soil water repellencyHYDROLOGICAL PROCESSES, Issue 15 2001J. Letey Abstract A wettable surface layer overlying a water-repellent layer is commonly observed following a fire on a watershed. High surface temperatures ,burn' off organic materials and create vapours that move downward in response to a temperature gradient and then condense on soil particles causing them to become water repellent. Water-repellent soils have a positive water entry pressure hp that must be exceeded or all the water will runoff. Water ponding depths ho that exceeds hp will cause infiltration, but the profile is not completely wetted. Infiltration rate and soil wetting increase as the value of ho/hp increases. The consequence is very high runoff, which also contributes to high erosion on fire-induced water-repellent soils during rain storms. Grass establishment is impaired by seeds being eroded and lack of soil water for seeds that do remain and germinate. Extrapolation of these general findings to catchment or watershed scales is difficult because of the very high temporal and spatial variabilities that occur in the field. Copyright © 2001 John Wiley & Sons, Ltd. [source] Application of Insect Repellents by Travelers to Malaria Endemic AreasJOURNAL OF TRAVEL MEDICINE, Issue 4 2006Yvonne Thrower MSc Background The use of insect repellents applied to the skin is always encouraged in those visiting malaria endemic areas. There are a number of factors contributing to the efficacy of insect repellents in the field and an important factor contributing to their longevity relates to the applied dose. This study investigated the dose of insect repellent that travelers might normally apply to the skin. Methods Subjects were asked to apply a product they had purchased to the arms and neck, and the dose applied (mg/cm2) was calculated. The study was conducted using three different populations; 74 travelers to malaria endemic areas visiting pharmacies to purchase repellents before they left the UK, 23 travelers on safari in India, and 24 on safari in Kenya. Results There was a significant difference in the mean applied arm dose of product (mg/cm2) [p < 0.001, analysis of variance (ANOVA)]; UK 1.15 (SD 0.29), India 0.96 (SD 0.23), and Kenya 1.34 (SD 0.54). Higher concentrations were achieved on the neck area. Few of the participants in the field trial used long-sleeved shirts at night. Even using 50% wt/vol repellent products, 32% in the Kenya and 25% in the India, study achieved a dose of active ingredient less than 0.5 mg/cm2. Conclusion These pilot studies would indicate that travelers tend to apply below the optimal concentration of active ingredient of repellent as might be suggested by mathematical models. [source] A Dengue Outbreak among Camp Participants in a Caribbean Island, 1995JOURNAL OF TRAVEL MEDICINE, Issue 2 2000Rob Lyerla Background: Dengue, a mosquito-transmitted viral disease, is a risk for visitors in tropical and subtropical areas. Several participants in a community-assistance program in Tortola, British Virgin Islands, in August, 1995, reported dengue-like symptoms either before or soon after leaving the island. Methods: We conducted a retrospective cohort study to determine the extent of the outbreak, risk factors for illness, and the proportion of inapparent infections. Program participants were interviewed by telephone or mail, and asked to submit a serum sample for dengue diagnosis. A clinically-diagnosed case of dengue was defined as a person with fever and two or more of the following: headache, retro-orbital pain, myalgia, arthralgia, rash, or hemorrhagic manifestations. Serum specimens were tested for virus isolation, polymerase chain reaction (PCR), plaque-reduction neutralization (PRNT) or anti-dengue IgM and IgG antibody. Results: Thirty-two (97%) of the 33 program participants responded; 21 of the 32 (66%) provided at least one serum sample for study. The median age was 17 years; 20 (62%) were women. Of 32 respondents, 22 (69%) met the clinical case definition for dengue: 15 of them (68%) had a positive IgM antibody response and 7 did not submit a serum sample. Dengue 1 virus (DEN-1) was identified by PCR in one case and all 11 positive PRNT results. No asymptomatic infections were identified. No respondent used effective mosquito repellent, and only 2 (6%) used bednets. Conclusion: A DEN-1 outbreak with a high attack rate (69%) occurred in a group of young short-term community aid workers. There were no asymptomatic infections documented. Participants' rare use of bednets or effective mosquito repellent highlights the importance of providing travelers to tropical areas with information about dengue fever and the recommended precautions to protect against infection. [source] Wildfire effects on soil erodibility of woodlands in NW SpainLAND DEGRADATION AND DEVELOPMENT, Issue 2 2010M. E. Varela Abstract Knowledge of soil erodibility following wildfire is of crucial importance for prioritisation of post-fire restoration practices for soil erosion mitigation. The present work therefore aims to determine the effect of wildfire on soil erodibility for common woodlands in Galicia, NW Spain. This is done by comparing selected topsoil properties of 28 pairs of recently wildfire-burned and neighbouring unburned sites on different geologic substrates. The soil properties were selected for their supposed importance in erodibility, and include aggregate size distribution and water aggregate stability. Comparison of the burned and unburned sites suggested that wildfire had a noticeable negative effect on aggregate size distribution but not on particle size distribution. The effect on aggregate stability was highly variable. Aggregate stability was clearly lower at the burned than unburned site in about a third of the cases and in the remaining cases either basically the same at both sites or higher at the unburned site. The differences in aggregate stability, like those in aggregate size distribution, appear to be associated with changes in organic carbon content. The impact of wildfire on soil erodibility is supposed to operate through its effect on soil organic matter and, thus, to depend strongly on fire severity. Soil erodibility is then little affected by low-severity wildfires but markedly diminished following high-severity wildfires. All burned topsoils were strongly to very strongly water repellent. Fire-enhaced repellency is therefore viewed as a key factor in the post-fire runoff and erosion processes repeatedly observed in recently burned areas in NW Spain. Copyright © 2009 John Wiley & Sons, Ltd. [source] Laboratory and field evaluation of insect repellents as oviposition deterrents against the mosquito Aedes albopictusMEDICAL AND VETERINARY ENTOMOLOGY, Issue 2 2001R.-D. Xue Abstract. Three experimental approaches were used to evaluate the oviposition deterrency of three insect repellents, AI3-35765, AI3-37220 (piperidine compounds), and the standard N,N -diethyl-3-methylbenzamide (deet) to the mosquito Aedes albopictus Skuse (Diptera: Culicidae). Against laboratory-reared Ae. albopictus gravid females, the EC50 values of AI3-37220, AI3-35765 and deet were 0.004%, 0.008% and 0.011% in laboratory cages and 0.004%, 0.01% and 0.009% in an outdoor screened cage. For a natural population of Ae. albopictus tested in the field, the EC50 values were determined as 0.004%, 0.008% and 0.001%, respectively. Ageing concentrations of 0.1% of each repellent provided >,50% effective oviposition deterrency against the laboratory population of Ae. albopictus for 13 days in laboratory cages, for 15 days in the outdoor cage, and for 21 days against field population of Ae. albopictus in Florida. These topical skin repellents are effective oviposition deterrents for Ae. albopictus when employed at relatively low application rates. [source] Toxic and repellent effects of Baccharis salicifolia essential oil on Tribolium castaneumPEST MANAGEMENT SCIENCE (FORMERLY: PESTICIDE SCIENCE), Issue 6 2005Matías García Abstract Allelochemical effects were observed when Tribolium castaneum (Herbst) adults were treated with Baccharis salicifolia (Ruiz & Pavon) Pers essential oil. The main biological activities were toxicity and repellence. Terpenes present in the essential oil were identified by GC-MS, and some authentic samples were tested to assess their activity individually. The most acutely toxic compounds after 3 days were ,-pinene and pulegone. Most of the monoterpenes elicited symptoms indicative of neurotoxicity. The most repellent compound was ,-terpineol. Toxic and repellent effects of chemical derivatives of the major sesquiterpene present in B salicifolia essential oil, as well as a series of monoterpenes, were evaluated in order to investigate structure-activity relationships. The reduced derivatives of the monoterpenes and sesquiterpenes were more repellent that their carbonyl analogues. In addition, unsaturation in the germacrane skeleton enhanced repellent activity. Copyright © 2005 Society of Chemical Industry [source] The behavioural response of slugs and snails to novel molluscicides, irritants and repellents,PEST MANAGEMENT SCIENCE (FORMERLY: PESTICIDE SCIENCE), Issue 12 2004Ingo Schüder Abstract The behavioural response of the slug Deroceras panormitanum (Lessona and Pollonera) and the snail Oxyloma pfeifferi (Rossmässler) to novel molluscicides was investigated in choice and no-choice experiments. Low-light video-recording in combination with automated tracking and event recording was used to identify the repellent and irritant effects of (1) cinnamamide, (2) copper ammonium carbonate, (3) a mulch, (4) a horticultural ground-cover matting impregnated with a copper formulation and (5) urea/formaldehyde. In the no-choice experiments the products had a stronger irritant effect on the snails than on the slugs. All products tested except the mulch significantly reduced the locomotor activity of both the slugs and snails. The most effective product, cinnamamide, reduced snail locomotor activity by 94% and track length by 96%. The overall repellent effect of the treatments in the choice experiments was stronger in the slugs; where presence, locomotor activity and track length in the treated area were significantly reduced by all products. The avoidance of treated areas exceeded 95% with the mulch (for slugs) and with copper ammonium carbonate (for snails). Copyright © 2004 Society of Chemical Industry [source] Pheromones in relation to aggregation and reproduction in desert locustsPHYSIOLOGICAL ENTOMOLOGY, Issue 1 2003Hans-Jörg Ferenz Abstract. Desert locusts, Schistocerca gregaria (Forskål) (Orthoptera: Acrididae), exhibit a population density-dependent phase polymorphism which includes the gradual change of many morphological, physiological and behavioural characteristics. Many volatiles associated with desert locusts have been identified recently and it is assumed that they are involved in pheromonal control of behaviour and development of locusts. Ovipositing females deposit with their egg pods several volatiles that appear to be attractive to other females resulting , possibly in combination with environmental factors , in an aggregated oviposition. Mature males release several volatiles, among them phenylacetonitrile, which are reported to accelerate sexual maturation in young males. Also, aggregation pheromone systems for hoppers and adults have been described. However, recent studies and publications shed a new light on the postulated effects of some of these volatiles. Gregarious behaviour can undoubtedly be induced by mechanical stimuli. Furthermore, the main component of the adult aggregation pheromone system, phenylacetonitrile, is found to be a repellent obviously not involved in aggregation. Comprehensive studies have demonstrated that phenylacetonitrile is used by mature gregarious males as a courtship inhibition pheromone to enhance mate guarding. Recent progress, contradictory results and perspectives in desert locust pheromone research related to reproduction are summarized and discussed in this paper. [source] Statistical modelling of insect behavioural responses in relation to the chemical composition of test extractsPHYSIOLOGICAL ENTOMOLOGY, Issue 4 2001Alan Hern Abstract. The use of generalized linear models (GLM) for relating changes in insect behaviour to changes in the chemical composition of a plant extract is presented and applied to data from an experimental study of the olfactory response of Cydia pomonella L. (Lepidoptera: Tortricidae) to apple volatiles. The volatiles were collected from healthy apples, artificially damaged apples or apples infested with C. pomonella larvae (either instar I, IV or V). These treatments produced a blend of 23 major components and the chemical composition of the blends differed substantially amongst the treatments. A statistically significant relationship was found between the concentration of hexyl hexanoate and 2-methylbutyl acetate in each extract and the number of moths moving upwind. Statistically significant models were developed which suggested that a relationship exists between the concentration of Z,E -,-farnesene, hexyl hexanoate and 2-methylbutyl acetate and the number and duration of movements made by the moths. Subsequently Y-tube assays were carried out to validate the predictions made with respect to the orientation of mated female C. pomonella. The results of these assays confirm hexyl hexanoate as an attractant. There were indications that 2-methylbutyl acetate acted as a repellent although differences were not statistically significant. Previous bioassays have shown that C. pomonella displays a statistically significant negative linear dose,response to ,-farnesene (Hern & Dorn, 1999). The statistical methods employed are very flexible and fairly easy to implement, offering the potential to screen plant extracts for bioactive compounds with a minimum of biological constraints. Their general applicability has yet to be demonstrated and as such these analyses only offer evidence of statistical relationships; the results must be validated by additional bioassays before conclusions can be drawn. [source] Electrophysiological and behavioural identification of host kairomones as olfactory cues for Culicoides impunctatus and C. nubeculosusPHYSIOLOGICAL ENTOMOLOGY, Issue 1 2000A. Bhasin Summary Electroantennograms (EAGs) were recorded from wild-caught parous, female Culicoides impunctatus (Goetghebuer) in response to components of host odour. Nine synthetic compounds were found to be electrophysiologically active, eliciting EAGs which were significantly different from solvent control. An EAG hierarchy was established, in which 1-octen-3-ol elicited the highest amplitude EAGs, followed by acetone, lactic acid and butanone. The overall responses to phenolic compounds were reduced compared to the non-phenolics. Subsequent behavioural analyses of the effects of these compounds when tested singly revealed 1-octen-3-ol, acetone and butanone to be attractive over specific stimulus doses. Exposure to supra-optimal doses modified the insects' behaviour; insects either ceased to respond or were repelled. Lactic acid was attractive at the lowest dose tested but was repellent at high doses. Behavioural responses to the phenolic components of host odour and lactic acid were similar, generally causing arrestment at low doses and repelling at the higher doses tested. A comparison of EAG profiles and behavioural assays between laboratory-reared Culicoides nubeculosus (Meigen) and C. impunctatus suggested that the same kairomones are utilized by both species, with C. nubeculosus being less sensitive than C. impunctatus. The EAG hierarchy of C. nubeculosus to the four non-phenolics was identical to that of C. impunctatus. [source] Soluble neuropilin-2, a nerve repellent receptor, is increased in rheumatoid arthritis synovium and aggravates sympathetic fiber repulsion and arthritisARTHRITIS & RHEUMATISM, Issue 10 2009Alexander Fassold Objective In inflammatory lesions, sympathetic nerve fibers disappear soon after the start of inflammation. We identified sympathetic nerve repellents as possible causal agents in rheumatoid arthritis (RA). On nerve terminals, repellent factors bind to neuropilin-2 and its coreceptor. The aim of this study was to investigate the role of neuropilin-2 in the synovial tissue of patients with RA and patients with osteoarthritis (OA) and in experimental arthritis. Methods The density of neuropilin-2,positive fibers and cells positive for semaphorin 3F (a sympathetic repellent) was investigated using immunofluorescence staining. Enzyme-linked immunosorbent assay was used to detect soluble neuropilin-2 in body fluids from patients with RA and patients with OA. An axon outgrowth assay and a neuropilin-2 Fc fusion construct (neuropilin-2Fc) were used to investigate semaphorin 3F,induced sympathetic nerve repulsion. In an animal model of type II collagen,induced arthritis, soluble neuropilin-2Fc was studied in vivo. Results The synovial density of neuropilin-2,positive sympathetic nerve fibers was lower in RA than in OA, but the density of cells positive for semaphorin 3F was similar. In synovial fluid, the level of soluble neuropilin-2 was markedly higher in RA compared with OA. Mouse sympathetic ganglia served as an excellent model with which to study semaphorin 3F,induced nerve fiber repulsion. Neuropilin-2 and its coreceptor were present on sympathetic neurons, and semaphorin 3F bound to neuropilin-2Fc (binding constant 96 nmoles/liter). Semaphorin 3F dose-dependently increased sympathetic nerve fiber repulsion (at a 50% maximum response concentration of 160,210 nmoles/liter). In contrast to our expectations, soluble neuropilin-2Fc did not inhibit repulsion but increased the repellent effect of semaphorin 3F. In experimental arthritis, therapy with neuropilin-2Fc aggravated arthritis. Conclusion Soluble neuropilin-2 has no antirepellent activity but aggravates sympathetic nerve fiber repulsion and arthritis. Increased shedding of neuropilin-2 is probably an unfavorable sign in RA. [source] Insect repellents and sunscreen: implications for personal protection strategies against mosquito-borne diseaseAUSTRALIAN AND NEW ZEALAND JOURNAL OF PUBLIC HEALTH, Issue 5 2009Cameron E. Webb Abstract Objective: To determine the protection times provided by insect repellent and sunscreen in combined formulations against biting mosquitoes. To determine if concurrent use of repellent and sunscreen influenced protection times. Methods: Insect repellent containing comparable concentrations of N, N-diethyl-3-methylbenzamide (DEET) with and without sunscreen were tested on human skin to determine the mean protection time (MPT) against Aedes aegypti (L.) in the laboratory. Further trials were undertaken to determine the effect on MPT of sunscreen reapplication over repellent every two hours. Results: There was no significant difference in the MPT provided by 80% DEET with (MPT±SE=770±54.8 minutes) and without (MPT±SE=830±20.2 minutes) sunscreen or 7.14% DEET with (MPT±SE =240±15.5 minutes) and 6.98% DEET without (MPT±SE =230±18.4 minutes) sunscreen. Reapplication of sunscreen resulted in a significantly lower MPT of a 17.0% DEET formulation when sunscreen was reapplied concurrently (MPT±SE=330±25.2 minutes), compared with DEET alone (MPT±SE =400±12.7 minutes). Conclusions: When combined in a single formulation with sunscreen, the MPT provided by both high and low concentrations of DEET is not reduced. However, if sunscreen is reapplied over insect repellent, protection times can be reduced significantly. Implications: In areas of endemic mosquito-borne disease, the reapplication of a low concentration repellent and sunscreen formulation may provide the most effective protection from biting mosquitoes while minimising the risk of overexposure to DEET. [source] Development of a synthetic plant volatile-based attracticide for female noctuid moths.AUSTRALIAN JOURNAL OF ENTOMOLOGY, Issue 1 2010Abstract This paper reports olfactometer studies to determine the attractiveness of synthetic equivalents of plant volatiles to Helicoverpa armigera (Hübner) moths. Synthetic volatiles identified from host plants and other volatiles reported in literature as attractants to various noctuid species were tested in a two-choice olfactometer. Of 34 single chemicals tested, only seven were significantly attractive, and six were significantly repellent. However, when presented as blends of two or more volatiles, 21 of 31 blends tested were significantly attractive, and only one was significantly repellent. The most attractive blends were those containing four to six components, including aromatic volatiles primarily found in flowers, especially 2-phenylethanol and phenylacetaldehyde and volatiles found primarily in leaves, including green leaf volatiles and terpenoids. Some general principles in blending volatiles for developing attracticides for H. armigera are presented, including the need for blend complexity, the combination of volatiles from leaves and flowers, and early consideration of pragmatic factors such as price and toxicological profile. [source] Development of Melaleuca oils as effective natural-based personal insect repellentsAUSTRALIAN JOURNAL OF ENTOMOLOGY, Issue 1 2010Kerryn A Greive Abstract For many decades effective insect repellents have relied on synthetic actives such as N,N -diethyl- meta -toluamide. Increasingly, consumers are seeking natural-based alternatives to many everyday products including insect repellents. While many studies have been published detailing the potential of essential oils to act as insect repellents, few oils have been identified as viable alternatives to synthetic actives. This study details the process involved in the selection of Australian essential oils effective as repellents and the subsequent testing of natural-based insect repellents using the selected oils. Using a combination of laboratory-based and field-based testing, oil from Melaleuca ericifolia was identified as being an effective insect repellent. When formulated into three different bases: an alcohol-based spray, an emulsion and a gel, these Melaleuca -based repellents were shown to be as effective at repelling mosquitoes Aedes vigilax (Skuse) (Diptera: Culicidae) and Verrallina carmenti (Edwards) (Diptera: Culicidae), the bush fly Musca vetustissima (Walker) (Diptera: Muscidae), and biting midges Culicoides ornatus (Taylor) (Diptera: Ceratopogonidae) and Culicoides immaculatus (Lee & Reye) (Diptera: Ceratopogonidae) as a synthetic-based commercial repellent. This study has shown that effective insect repellents based on natural active ingredients can deliver repellency on par with synthetic actives in the field. Three Melaleuca -based formulations have been registered as repellents and are now commercially available. [source] Design and Analysis of Arm-in-Cage Experiments: Inference for Three-State Progressive Disease Models with Common Periodic Observation TimesBIOMETRICS, Issue 2 2008B. A. Griffin Summary We develop statistical methods for designing and analyzing arm-in-cage experiments used to test the efficacy of insect repellents and other topical treatments. In these experiments, a controlled amount of the treatment is applied to a volunteer's forearm, which then is exposed to the insects by being placed into a special cage. Arms are not kept in the cages continuously, but rather placed there periodically for a brief period of time, during which it is noted whether an insect lands (but does not bite) or (lands and) bites. Efficacy of a repellent can be described using a progressive three-state model in which the first two states represent varying degrees of protection (no landing and landing without biting) and the third state occurs once protection is completely lost (biting). Because subjects within a treatment group follow the same cage visit schedule, transition times between states are interval censored into one of several fixed intervals. We develop an approach that uses a mixture of nonparametric and parametric techniques for estimating the parameters of interest when sojourn times are dependent. Design considerations for arm-in-cage experiments are addressed and the proposed methods are illustrated on data from a recent arm-in-cage experiment as well as simulated data. [source] Effects of steroid hormones on five functional parameters of Tetrahymena: evolutionary conclusionsCELL BIOCHEMISTRY AND FUNCTION, Issue 1 2003László K, hidai Abstract The unicellular Tetrahymena pyriformis was studied for chemotaxis, chemotactic selection, phagocytosis, growth and body shape changes in the presence of water soluble (,-cyclodextrin-coupled) steroid hormones (testosterone, estradiol, progesterone, hydrocortisone and dexamethasone). Testosterone was chemoattractant over a wide range of concentrations, while progesterone and dexamethasone were active only at one concentration (10,5 and 10,6,mg,ml,1 respectively) and were either neutral or repellent at other concentrations. Hydrocortisone and estradiol were unambiguously chemorepellent. Chemotactic selection enhanced the effect of testosterone and estradiol, while in the case of hydrocortisone the action was reversed. The other parameters were mildly influenced by the steroid hormones. The results call attention to the fine molecular recognition capacity of Tetrahymena and to the possible rapid effects of steroid hormones at membrane receptors at a very low evolutionary eukaryotic level. Copyright © 2002 John Wiley & Sons, Ltd. [source] Protection of lodgepole pines from mass attack by mountain pine beetle, Dendroctonus ponderosae, with nonhost angiosperm volatiles and verbenoneENTOMOLOGIA EXPERIMENTALIS ET APPLICATA, Issue 2 2001D.P.W. Huber Abstract A number of angiosperm nonhost volatiles (NHVs) and green leaf volatiles (GLVs) were tested alone and as supplements to the antiaggregation pheromone, verbenone, for their ability to disrupt attack by the mountain pine beetle (MPB), Dendroctonus ponderosae Hopkins (Coleoptera: Scolytidae), on lodgepole pine, Pinus contorta Dougl. ex Loud. var. latifolia Engel. Preliminary experiments led to a refined NHV blend [benzyl alcohol, guaiacol, benzaldehyde, nonanal, salicylaldehyde, and conophthorin] and a refined GLV blend [(Z)-3-hexen-1-ol and (E)-2-hexen-1-ol]. In a 20-replicate experiment, NHV and GLV groups both singly, and verbenone alone, significantly reduced MPB mass attack on pheromone-baited trees and on trees within 5 m of the pheromone-baited trees. Both blends in combination with verbenone reduced the number of mass attacked, baited trees to three out of 20 compared to 20 out of 20 of the baited controls. Each binary combination was also effective at reducing mass attack. In these experiments, all tested repellents were released from devices stapled to trees at the same point as the pheromone bait, suggesting that the repellency could have been to a point source, rather than to the whole tree. Therefore, in two further experiments bands of release devices were wrapped around the treated trees and the pheromone bait was removed from the treated trees. In one experiment, when the aggregation pheromone bait was suspended between pairs of trees treated with the NHV blend plus GLV blend plus verbenone, only three out of 25 treated pairs had mass attack on at least one member of the pair. In the other 60-replicate experiment, with no pheromone baits present, attack occurred on 13 untreated and 11 banded trees, all in the path of a large advancing infestation. However, the mean attack density on the banded trees was significantly reduced to a level below the 40 attacks m,2 of bark surface required to kill a healthy lodgepole pine. As a result of these experiments, operational trials are recommended. [source] Self-produced extracellular stimuli modulate the Pseudomonas aeruginosa swarming motility behaviourENVIRONMENTAL MICROBIOLOGY, Issue 10 2007Julien Tremblay Summary Pseudomonas aeruginosa presents three types of motilities: swimming, twitching and swarming. The latter is characterized by rapid and coordinated group movement over a semisolid surface resulting from morphological differentiation and intercellular interactions. A striking feature of P. aeruginosa swarming motility is the formation of migrating tendrils producing colonies with complex fractal-like patterns. Previous studies have shown that normal swarming motility is intimately related to the production of extracellular surface-active molecules: rhamnolipids (RLs), composed of monorhamnolipids (mono-RLs) and dirhamnolipids (di-RLs), and 3-(3-hydroxyalkanoyloxy) alkanoic acids (HAAs). Here, we report that (i) di-RLs attract active swarming cells while HAAs behave as strong repellents, (ii) di-RLs promote and HAAs inhibit tendril formation and migration, (iii) di-RLs and HAAs display different diffusion kinetics on a surface as di-RLs spread faster than HAAs in agar, (iv) di-RLs and HAAs have no effect on swimming cells, suggesting that swarming cells are different from swimming cells not only in morphology but also at the regulatory level and (v) mono-RLs act as wetting agents. We propose a model explaining how HAAs and di-RLs together modulate the behaviour of swarming migrating cells by acting as self-produced negative and positive chemotactic-like stimuli. 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