Rapid Transport (rapid + transport)

Distribution by Scientific Domains


Selected Abstracts


Live-cell analysis of mitotic spindle formation in taxol-treated cells

CYTOSKELETON, Issue 8 2008
Jessica E. Hornick
Abstract Taxol functions to suppress the dynamic behavior of individual microtubules, and induces multipolar mitotic spindles. However, little is known about the mechanisms by which taxol disrupts normal bipolar spindle assembly in vivo. Using live imaging of GFP-, tubulin expressing cells, we examined spindle assembly after taxol treatment. We find that as taxol-treated cells enter mitosis, there is a dramatic re-distribution of the microtubule network from the centrosomes to the cell cortex. As they align there, the cortical microtubules recruit NuMA to their embedded ends, followed by the kinesin motor HSET. These cortical microtubules then bud off to form cytasters, which fuse into multipolar spindles. Cytoplasmic dynein and dynactin do not re-localize to cortical microtubules, and disruption of dynein/dynactin interactions by over-expression of p50 "dynamitin" does not prevent cytaster formation. Taxol added well before spindle poles begin to form induces multipolarity, but taxol added after nascent spindle poles are visible,but before NEB is complete,results in bipolar spindles. Our results suggest that taxol prevents rapid transport of key components, such as NuMA, to the nascent spindle poles. The net result is loss of mitotic spindle pole cohesion, microtubule re-distribution, and cytaster formation. Cell Motil. Cytoskeleton 2008. © 2008 Wiley-Liss, Inc. [source]


Single- and dual-porosity modelling of multiple tracer transport through soil columns: effects of initial moisture and mode of application

EUROPEAN JOURNAL OF SOIL SCIENCE, Issue 1 2001
T. Kätterer
Summary We investigated the effect of initial moisture contents and mode of application on the displacement of multiple conservative tracers through undisturbed columns of a Humic Gleysol. Bromide was applied at the soil surface and chloride was injected at 5 cm depth. The columns were irrigated with deuterium-enriched water. A dual-porosity model and two single-porosity models were calibrated separately to Br, and Cl, elution curves in the two columns. Elution curves were almost identical for Br, and Cl, under initially wet conditions, whereas the displacement of Br, was faster than that of Cl, in the initially dry column, indicating rapid transport with preferential flow. Only the dual-porosity model described the long-tailing breakthrough of Cl, in the initially dry column adequately. The parameter values giving acceptable fits for ,Br dry' were not compatible with the description of the three other elution curves, which could be adequately modelled with a single set of parameter values. The estimated set of common parameters was validated by comparing with the elution curves of deuterium water, nitrate and sulphate, as well as with resident tracer concentrations at four depths. The results showed that solutes can be displaced much faster when applied at the surface of initially dry soil than when applied to wet soil or when resident in the soil matrix. The simulation results suggest that solute transport under initially dry conditions was governed by preferential flow of infiltration water through macropores by-passing the matrix due to shrinkage cracks and water repellence of matrix surfaces. [source]


Numerical modeling of hydrothermal zinc silicate and sulfide mineralization in the Vazante deposit, Brazil

GEOFLUIDS (ELECTRONIC), Issue 2 2009
M. S. APPOLD
Abstract The Vazante zinc deposit in central Brazil is currently the world's largest known example of a hypogene nonsulfide (i.e. willemite-dominant) zinc deposit. The mineralization is hypothesized to have formed as a result of mixing between a hot, acidic, reducing, metal-rich brine and a cool, more basic and dilute, metal-poor meteoric fluid. The present study sought to investigate this scenario by quantifying the individual effects of temperature, pH, salinity, and oxidation state on willemite and sphalerite solubility, and modeling their combined effects during mixing through reaction path and reactive transport modeling. Solubility calculations showed that in an initially hot, moderately acidic, reducing, metal-rich ore fluid saturated with respect to silica, willemite solubility is relatively insensitive to changes in temperature and log , but highly sensitive to changes in pH and salinity. In contrast, sphalerite solubility was highly sensitive to changes in temperature and log , as well as salinity, and was less sensitive than willemite to changes in pH. Reaction path models sought to extend these observations by modeling the geochemistry of mixing. The results show that mixing is able to produce most of the major zinc ore and gangue minerals observed in the field, though not necessarily at the same paragenetic stages, and that both compositional and temperature changes from mixing are needed. Reactive transport models were formulated to investigate spatial patterns of mineralization. The results showed that sphalerite deposition was strongly controlled by temperature and concentrated in the regions of greatest temperature change. Willemite deposition was concentrated along the interface between the metal-rich ore fluid and the surrounding meteoric fluid. The more rapid transport of solute than heat, in conjunction with the higher concentration of silica than sulfide in both fluids meant that willemite mineralization developed over a broader region and in greater concentrations compared with sphalerite. [source]


Karst Spring Responses Examined by Process-Based Modeling

GROUND WATER, Issue 6 2006
Steffen Birk
Ground water in karst terrains is highly vulnerable to contamination due to the rapid transport of contaminants through the highly conductive conduit system. For contamination risk assessment purposes, information about hydraulic and geometric characteristics of the conduits and their hydraulic interaction with the fissured porous rock is an important prerequisite. The relationship between aquifer characteristics and short-term responses to recharge events of both spring discharge and physicochemical parameters of the discharged water was examined using a process-based flow and transport model. In the respective software, a pipe-network model, representing fast conduit flow, is coupled to MODFLOW, which simulates flow in the fissured porous rock. This hybrid flow model was extended to include modules simulating heat and reactive solute transport in conduits. The application of this modeling tool demonstrates that variations of physicochemical parameters, such as solute concentration and water temperature, depend to a large extent on the intensity and duration of recharge events and provide information about the structure and geometry of the conduit system as well as about the interaction between conduits and fissured porous rock. Moreover, the responses of solute concentration and temperature of spring discharge appear to reflect different processes, thus complementing each other in the aquifer characterization. [source]