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Rapid Assembly (rapid + assembly)

Distribution by Scientific Domains
Chemistry85%

Selected Abstracts

SMILIB: Rapid Assembly of Combinatorial Libraries in SMILES Notation

MOLECULAR INFORMATICS, Issue 7 2003
Andreas Schüller
Abstract A software tool was developed for fast combinatorial library enumeration (SMILIB). Its particular features are its simplicity to use, high flexibility in constructing combinatorial libraries and high speed of library construction. SMILIB offers the possibility to construct very large combinatorial libraries using the flexible and portable SMILES format. Libraries are generated at rates of approximately 30,000 molecules per minute. Combinatorial building blocks are attached to scaffolds by means of linkers rather than to concatenate them directly. This allows for creation of easily customized libraries using linkers of different size and chemical nature. A web interface for a limited web-based version of the software is available at URL: www.modlab.de. An unlimited binary version of SMILIB for command line execution on Linux systems is available from this URL. [source]

A Triple-Aldol Cascade Reaction for the Rapid Assembly of Polyketides,

ANGEWANDTE CHEMIE, Issue 15 2010
Brian
Äußerst wenig Katalysator wird für die Titelreaktion benötigt, bei der 1 mit einfachen Aldehyden zu 3,5,7-Trisilyloxyaldehyden reagiert (siehe Schema). Iodbenzol erleichtert die dritte Aldolreaktion, offenbar indem es als Lewis-Base gegenüber dem Silylkatalysator wirkt. Tf=Trifluormethansulfonyl. [source]

ChemInform Abstract: Exploiting the Divergent Reactivity of Aryl,Palladium Intermediates for the Rapid Assembly of Fluorene and Phenanthrene Derivatives.

CHEMINFORM, Issue 25 2009
Ya-Bin Zhao
Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a "Full Text" option. The original article is trackable via the "References" option. [source]

Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin

CYTOSKELETON, Issue 11 2007
Brian M. Burkel
Abstract Actin filaments (F-actin) are protein polymers that undergo rapid assembly and disassembly and control an enormous variety of cellular processes ranging from force production to regulation of signal transduction. Consequently, imaging of F-actin has become an increasingly important goal for biologists seeking to understand how cells and tissues function. However, most of the available means for imaging F-actin in living cells suffer from one or more biological or experimental shortcomings. Here we describe fluorescent F-actin probes based on the calponin homology domain of utrophin (Utr-CH), which binds F-actin without stabilizing it in vitro. We show that these probes faithfully report the distribution of F-actin in living and fixed cells, distinguish between stable and dynamic F-actin, and have no obvious effects on processes that depend critically on the balance of actin assembly and disassembly. Cell Motil. Cytoskeleton 2007. © 2007 Wiley-Liss, Inc. [source]

First Asymmetric Total Synthesis of Penarolide Sulfate A1

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY, Issue 36 2008
Debendra K. Mohapatra
Abstract Penarolide sulfate A1, with three contiguous stereogenic centers on a macrocyclic skeleton, affords promise as an ,-glucosidase inhibitor. Herein, we describe the first asymmetric total synthesis of this natural product. A stereoselective strategy for rapid assembly of the complete framework of the 30-membered macrocyclic core is delineated herein. Sequential amidation and intramolecular Sonogashira cross-coupling reactions were pivotal to the success of our efforts. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008) [source]

Imine Additions of Internal Alkynes for the Synthesis of Trisubstituted (E)-Alkene and Cyclopropane Peptide Isosteres

ADVANCED SYNTHESIS & CATALYSIS (PREVIOUSLY: JOURNAL FUER PRAKTISCHE CHEMIE), Issue 11-13 2005
Peter Wipf
Abstract Divergent multi-component reactions (DMCR) involving CC bond formations can provide large increases in structural diversity and allow the rapid assembly of complex products from readily available starting materials. Cascade hydrozirconation-Zr/Zn transmetalation-imine addition of alkynes represents a versatile methodology for the synthesis of (E)-alkene and cyclopropane dipeptide isosteres. Appropriate substitutions at the sp2 -carbon of (E)-alkene peptide isosteres allow a range of Pd-catalyzed cross-coupling reactions, which can be used for the fine-tuning of the conformational and electronic properties of the parent peptide bond mimic. CC bond formation by microwave-accelerated Stille coupling of stannylalkenes represents a fast, convergent synthetic approach toward trisubstituted (E)-alkene dipeptide isosteres. [source]

Cloning large natural product gene clusters from the environment: Piecing environmental DNA gene clusters back together with TAR

BIOPOLYMERS, Issue 9 2010
Jeffrey H. Kim
Abstract A single gram of soil can contain thousands of unique bacterial species, of which only a small fraction is regularly cultured in the laboratory. Although the fermentation of cultured microorganisms has provided access to numerous bioactive secondary metabolites, with these same methods it is not possible to characterize the natural products encoded by the uncultured majority. The heterologous expression of biosynthetic gene clusters cloned from DNA extracted directly from environmental samples (eDNA) has the potential to provide access to the chemical diversity encoded in the genomes of uncultured bacteria. One of the challenges facing this approach has been that many natural product biosynthetic gene clusters are too large to be readily captured on a single fragment of cloned eDNA. The reassembly of large eDNA-derived natural product gene clusters from collections of smaller overlapping clones represents one potential solution to this problem. Unfortunately, traditional methods for the assembly of large DNA sequences from multiple overlapping clones can be technically challenging. Here we present a general experimental framework that permits the recovery of large natural product biosynthetic gene clusters on overlapping soil-derived eDNA cosmid clones and the reassembly of these large gene clusters using transformation-associated recombination (TAR) in Saccharomyces cerevisiae. The development of practical methods for the rapid assembly of biosynthetic gene clusters from collections of overlapping eDNA clones is an important step toward being able to functionally study larger natural product gene clusters from uncultured bacteria. © 2010 Wiley Periodicals, Inc. Biopolymers 93: 833,844, 2010. [source]