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Quiescent State (quiescent + state)
Selected AbstractsEvidence for neural stem cells in the medaka optic tectum proliferation zones,DEVELOPMENTAL NEUROBIOLOGY, Issue 10 2010Alessandro Alunni Abstract Few adult neural stem cells have been characterized in vertebrates. Although teleosts continually generate new neurons in many regions of the brain after embryogenesis, only two types of neural stem cells (NSCs) have been reported in zebrafish: glial cells in the forebrain resembling mammalian NSCs, and neuroepithelial cells in the cerebellum. Here, following our previous studies on dividing progenitors (Nguyen et al. [1999]: J Comp Neurol 413:385,404.), we further evidenced NSCs in the optic tectum (OT) of juvenile and adult in the medaka, Oryzias latipes. To detect very slowly cycling progenitors, we did not use the commonly used BrdU/PCNA protocol, in which PCNA may not be present during a transiently quiescent state. Instead, we report the optimizations of several protocols involving long subsequent incubations with two thymidine analogs (IdU and CldU) interspaced with long chase times between incubations. These protocols allowed us to discriminate and localize fast and slow cycling cells in OT of juvenile and adult in the medaka. Furthermore, we showed that adult OT progenitors are not glia, as they express neither brain lipid-binding protein (BLBP) nor glial fibrillary acidic protein (GFAP). We also showed that expression of pluripotency-associated markers (Sox2, Musashi1 and Bmi1) colocalized with OT progenitors. Finally, we described the spatio-temporally ordered population of NSCs and progenitors in the medaka OT. Hence, the medaka appears as an invaluable model for studying neural progenitors that will open the way to further exciting comparative studies of neural stem cells in vertebrates. © 2010 Wiley Periodicals, Inc. Develop Neurobiol 70: 693,713, 2010 [source] Demonstration of strong enterobacterial reactivity of CD4+CD25, T,cells from conventional and germ-free mice which is counter-regulated by CD4+CD25+ T,cellsEUROPEAN JOURNAL OF IMMUNOLOGY, Issue 3 2004Monika Gad Abstract Unfractionated CD4+ T,cells from the gut-associated lymphoid tissue (GALT) and peripheral lymph nodes are unresponsive when exposed to enterobacterial antigens in vitro. Under similar conditions, CD4+ T,cells depleted in vivo or in vitro of CD4+CD25+ T,cells proliferate extensively. The CD4+CD25, T,cell reactivity depends on MHC class,II presentation, specific TCR stimulation, CD4 ligation, and antigen processing by antigen-presenting cells. The CD4+CD25, T,cells respond to autologousand heterologous enterobacterial antigens, but not to antigens from the feces of germ-free mice. Surprisingly, CD4+CD25, T,cells obtained from the GALT of germ-free mice also proliferate when exposed to enterobacterial antigens, and adding back the conventional or germ-free CD4+CD25+ T,cells to the enteroantigen-stimulated CD4+CD25, T,cells abolishes proliferation. As judged from carboxyfluorescein diacetate succinimidyl ester-labeling experiments, 4,5% of the CD4+CD25, T,cells respond to enteroantigen. The data show for the first time that CD4+CD25, T,cells with reactivity towards the enterobacterial flora and regulatory CD4+CD25+ T,cells are present in both conventional and germ-free mice. The data suggest that a significant proportion of the peripheral pool of CD4+CD25, T,cells express anti-enterobacterial reactivity, which, due to the presence of regulatory CD4+CD25+ T,cells, is kept in a quiescent state. [source] Expression of a cyclin E1 isoform in mice is correlated with the quiescent cell cycle status of hepatocytes in vivo,HEPATOLOGY, Issue 1 2006Nils-Holger Zschemisch Cyclin E1 controls G1/S phase transition of the eukaryotic cell cycle. We report the impact of alternative spliced cyclin E1 isoforms on cell cycle regulation in hepatocytes. We show that expression of new cyclin E1 mRNA variants IN3, ,4, and ,5 is associated with retarded proliferation in murine hepatocellular carcinoma. Additionally, we demonstrate that a new cyclin E1 isoform ,3/8 lacking the central part of wild-type mRNA is expressed predominantly in nonproliferating murine hepatocytes. Following partial hepatectomy, ,3/8 is downregulated when hepatocytes enter the cell cycle from quiescence. The ,3/8 protein does not exhibit any cyclin box motif but binds cyclin-dependent kinase 2 without stimulating kinase activity. We demonstrate that ,3/8 lacks any nuclear localization signal and is exclusively located in the cytoplasm. Overexpression of ,3/8 in cultured cells leads to a delayed G0-G1 transition, indicating that this splice variant helps to maintain a quiescent state of hepatocytes. In conclusion, we identified an isoform of cyclin E1 involved in G0 maintenance and suggest an additional mechanism for cell cycle control. (HEPATOLOGY 2006;44:164,173.) [source] Mirk/Dyrk1B in cancerJOURNAL OF CELLULAR BIOCHEMISTRY, Issue 2 2007Eileen Friedman Abstract Mirk/Dyrk1B is a member of a conserved family of serine/threonine kinases which are activated by intramolecular tyrosine phosphorylation, and which mediate differentiation in different tissues,Mirk in skeletal muscle, Dyrk1A in the brain, etc. One role of Mirk in skeletal muscle differentiation is to block cycling myoblasts in the G0 quiescent state by modification of cell cycle regulators, while another role of Mirk is to limit apoptosis in fusing myoblasts. Amplification of the Mirk gene, upregulation of Mirk expression and/or constitutive activation of this kinase have been observed in several different types of cancer. If coupled with a stress condition such as serum starvation which induces a quiescent state, depletion of Mirk by RNA interference using either synthetic duplex RNAi's or pSilencer-encoded RNAi's have decreased colony formation of different cancer cell lines and enhanced apoptosis induced by chemotherapeutic drugs. Mirk is activated by phosphorylation by the stress-activated SAPK kinases MKK3 and MKK6. Our working hypothesis is that Mirk is activated by this pathway in response to various stresses, and then acts as a checkpoint kinase to arrest damaged tumor cells in a quiescent state and allow cellular repair. Pharmacological inhibition of Mirk may enhance the anti-tumor effect of chemotherapeutic drugs. J. Cell. Biochem. 102: 274,279, 2007. © 2007 Wiley-Liss, Inc. [source] Nuclear accumulation of glycogen synthase kinase-3 during replicative senescence of human fibroblastsAGING CELL, Issue 5 2004Jaroslaw W. Zmijewski Summary Activation of the tumor suppressor protein p53 contributes to cellular senescence. As glycogen synthase kinase-3 (GSK3) was recently found to interact with p53 and contribute to the actions of p53, this study examined whether GSK3 accumulated in the nucleus and associated with p53 in senescent cells. Compared with young and middle-aged human WI-38 fibroblasts, senescent cells were found to contain increased nuclear levels of GSK3,, and also tended to accumulate in the nucleus the other isoform of GSK3, GSK3,. Co-immunoprecipitation experiments demonstrated that GSK3, and p53 formed a complex in the nucleus. Further experiments tested whether inhibition of GSK3 altered the development of senescence using long-term treatment with the selective GSK3 inhibitor lithium. Lithium treatment reduced the senescence-associated accumulation of p53 and caused cells to enter a reversible quiescent state. These results indicate that a portion of the p53 that is activated in senescent cells is modulated by its association with GSK3, in the nucleus, an association that is known to facilitate the actions of p53 and that may contribute to senescence. [source] SEDIMENTARY IMPRINT OF MICROCYSTIS AERUGINOSA (CYANOBACTERIA) BLOOMS IN GRANGENT RESERVOIR (LOIRE, FRANCE),JOURNAL OF PHYCOLOGY, Issue 3 2007Delphine Latour Analysis of a sediment core taken from the Grangent reservoir in 2004 showed the presence of high concentrations of Microcystis aeruginosa Kütz. colonies at the sediment surface (250 colonies,·,mL sediment,1) and also at depths of 25,35 cm (2300 colonies·mL sediment,1) and 70 cm (600 colonies,·,mL sediment,1). Measurements of radioactive isotopes (7Be, 137Cs, and 241Am) along with photographic analysis of the core were used to date the deep layers: the layer located at ,30 cm dates from summer 2003, and that located at ,70 cm from 1990 to 1991. The physiological and morphological conditions of those benthic colonies were compared with those of planktonic colonies using several techniques (environmental scanning electron microscopy [ESEM], TEM, DNA markers, cellular esterases, and toxins). The ESEM observations showed that, as these colonies age, peripheral cells disappear, with no cells remaining in the mucilage of the deepest colonies (70 cm), an indication of the survival thresholds of these organisms. In the benthic phase, the physiological conditions (enzyme activity, cell division, and intracellular toxins) and ultrastructure (particularly the gas vesicles) of the cells surviving in the heart of the colony are comparable to those of the planktonic form, with all the potential needed for growth. Maintaining cellular integrity requires a process that can provide sufficient energy and is expressed in the reduced, but still existing, enzymatic activity that we measured, which is equivalent to a quiescent state. [source] The molecular basis of factor V and VIII procofactor activationJOURNAL OF THROMBOSIS AND HAEMOSTASIS, Issue 12 2009R. M. CAMIRE Summary., Activation of precursor proteins by specific and limited proteolysis is a hallmark of the hemostatic process. The homologous coagulation factors (F)V and FVIII circulate in an inactive, quiescent state in blood. In this so-called procofactor state, these proteins have little, if any procoagulant activity and do not participate to any significant degree in their respective macromolecular enzymatic complexes. Thrombin is considered a key physiological activator, cleaving select peptide bonds in FV and FVIII which ultimately leads to appropriate structural changes that impart cofactor function. As the active cofactors (FVa and FVIIIa) have an enormous impact on thrombin and FXa generation, maintaining FV and FVIII as inactive procofactors undoubtedly plays an important regulatory role that has likely evolved to maintain normal hemostasis. Over the past three decades there has been widespread interest in studying the proteolytic events that lead to the activation of these proteins. While a great deal has been learned, mechanistic explanations as to how bond cleavage facilitates conversion to the active cofactor species remain incompletely understood. However, recent advances have been made detailing how thrombin recognizes FV and FVIII and also how the FV B-domain plays a dominant role in maintaining the procofactor state. Here we review our current understanding of the molecular process of procofactor activation with a particular emphasis on FV. [source] Keck infrared observations of GRO J0422+32 in quiescenceMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 2 2007Mark T. Reynolds ABSTRACT We present Keck K -band photometry and low-resolution H - and K -band spectroscopy of the X-ray nova GRO J0422+32 obtained while the system was in the quiescent state. No clear ellipsoidal modulation is present in the light curve, which is instead dominated by a strong flickering component. In the K band, we observe strong Br, emission, with an equivalent width of 38 ± 5 Ĺ. From this, we conclude that the accretion disc is the most likely source of the observed photometric contamination, and that previous infrared-based attempts to constrain the mass of the putative black hole in this system are prone to considerable uncertainty. We finally proceed to show how it is possible to place meaningful constraints on some of the binary parameters of this system, even in the presence of a relatively high level of contamination from the disc. [source] The stellar mass ratio of GK PerseiMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 3 2002L. Morales-Rueda We study the absorption lines present in the spectra of the long-period cataclysmic variable GK Per during its quiescent state, which are associated with the secondary star. By comparing quiescent data with outburst spectra we infer that the donor star appears identical during the two states and the inner face of the secondary star is not noticeably irradiated by flux from the accreting regions. We obtain new values for the radial velocity semi-amplitude of the secondary star, , a projected rotational velocity, and consequently a measurement of the stellar mass ratio of GK Per, . The inferred white dwarf radial velocities are greater than those measured traditionally using the wings of Doppler-broadened emission lines suspected to originate in an accretion disc, highlighting the unsuitability of emission lines for mass determinations in cataclysmic variables. We determine mass limits for both components in the binary, and . [source] Signaling satellite-cell activation in skeletal muscle: Markers, models, stretch, and potential alternate pathwaysMUSCLE AND NERVE, Issue 3 2005Ashley C. Wozniak BSc Abstract Activation of skeletal muscle satellite cells, defined as entry to the cell cycle from a quiescent state, is essential for normal growth and for regeneration of tissue damaged by injury or disease. This review focuses on early events of activation by signaling through nitric oxide and hepatocyte growth factor, and by mechanical stimuli. The impact of various model systems used to study activation and the regulation of satellite-cell quiescence are placed in the context of activation events in other tissues, concluding with a speculative model of alternate pathways signaling satellite-cell activation. Muscle Nerve, 2005 [source] Development of a PCR test to detect the downy mildew causal agent Plasmopara halstedii in sunflower seedsPLANT PATHOLOGY, Issue 2 2007R. Ioos Plasmopara halstedii, the causal agent of downy mildew of sunflower, is an obligate parasite but viable sporangia and oospores of the pathogen may be found in a quiescent state in seeds of sunflower and therefore may be transported with sunflower seeds in international commercial exchanges. In order to prevent the spread of this pathogen, especially the introduction of potentially new races, an efficient method to analyse sunflower seed samples is required. In this study, a P. halstedii -specific polymerase chain reaction (PCR) test was developed based on the ribosomal large sub unit (LSU) DNA. The forward (PHAL-F) and reverse (PHAL-R) PCR primers were designed from two polymorphic regions of LSU. After screening 22 isolates of P. halstedii corresponding to different races and countries and 32 other oomycete, deuteromycete and ascomycete isolates, the PHAL-F/R primers amplified only a single PCR band of c. 310 bp from P. halstedii. The PHAL-F/R PCR test could detect as little as 3 pg of P. halstedii genomic DNA per 20 µL reaction volume and enabled the direct detection of P. halstedii in 35 g sunflower seed samples without the need for any prior biological baiting step. An internal amplification control (IAC) was developed to help discriminate against false negative samples due to the potential presence of inhibitory compounds in DNA extracts. The test was successfully used on samples of naturally contaminated seeds. These new molecular tools should be of great interest for quarantine seed testing purposes. [source] Heat transfer behavior of melting polymers in laminar flow fieldPOLYMER ENGINEERING & SCIENCE, Issue 3 2004Sadao Sato Heat transfer coefficients were investigated by insertion of a probe into melting polymers under laminar flow at 200,240°C and a flow velocity of 0.5,2.7 mm/sec. The average heat transfer coefficients of melting polypropylene (PP) and polystyrene (PS) were found to be 160,220 W/m·°C and 180,270 W/m·°C, respectively. These coefficients show remarkable dependence on flow velocity, and the average heat transfer coefficient of PS is about 13%,23% higher than that of PP. When the flow velocity of flowing melting PP and PS exceeds about 0.078mm/sec, heat transfer by convection becomes dominant, whereas under lower flow velocities, since the equivalent conduction layer thickness ,, in which the quiescent state without flow approaches infinity, heat transfer by conduction becomes dominant. The Prandtl number (Pr) and Nusselt number (Nu) of melting PP are 125,133 × 106 and 38.6,51.4, respectively, and those of melting PS are 63,64 × 106 and 42.3,61.3. In the case of constant flow velocity, the Peclet number (Pe) and Stanton number (St) are dependent on the specific heat of melting polymer. Polym. Eng. Sci. 44:423,432, 2004. © 2004 Society of Plastics Engineers. [source] Chandra ACIS Imaging Spectroscopy of Sgr A EastASTRONOMISCHE NACHRICHTEN, Issue S1 2003Y. Maeda Abstract We report on the X-ray emission from the shell-like, non-thermal radio source Sgr A East located in the inner few parsecs of the Galaxy based on observations made with the ACIS detector on board the Chandra X-ray Observatory. The X-ray emission from Sgr A East is concentrated within the central ,2 pc of the larger radio shell. The spectrum shows strong K, lines from highly ionized ions of S, Ar, Ca, and Fe. A simple isothermal plasma model gives electron temperature ,2 keV, absorption column ,1 × 1023 H cm,2, luminosity ,8 × 1034 ergs s,1 in the 2,10 keV band, and gas mass ,2,˝ M, with a filling factor ,. The plasma appears to be rich in heavy elements, over-abundant by roughly a factor of four with respect to solar abundances. Accompanied with filamentary or blob-like structures, the plasma shows a spatial gradient of elemental abundance: the spatial distribution of iron is more compact than that of the lighter elements. These Chandra results strongly support the long-standing hypothesis that Sgr A East is a supernova remnant (SNR). Since Sgr A East surrounds Sgr A* in projection, it is possible that the dust ridge compressed by the forward shock of Sgr A East hit Sgr A* in the past, and the passage of the ridge may have supplied material to accrete onto the black hole in the past, and may have removed material from the black hole vicinity, leading to its present quiescent state. [source] Limits on the Short Term Variability of Sagittarius A* in the Near-InfraredASTRONOMISCHE NACHRICHTEN, Issue S1 2003S. D. Hornstein Abstract The detection of X-ray flares by the Chandra X-ray Observatory and XMM-Newton has raised the possibility of enhanced emission over a broad range of wavelengths from Sagittarius A*, the suspected 2.6 × 106 M, black hole at the Galactic center, during a flaring event. We have, therefore, reconstructed 3,4 hr data sets from 2 ,m speckle and adaptive optics images (,core = 50,100 mas) obtained with the W. M. Keck 10 m telescopes between 1995 and 2002. The results for 25 of these observations were reported by Hornstein et al. (2002) and an additional 11 observations are presented here. In the 36 separate observations, no evidence of any significant excess emission associated with Sgr A* was detected. The lowest of our detection limits gives an observed limit for the quiescent state of Sgr A* of 0.09 ± 0.005 mJy, or, equivalently, a dereddened value of 2.0 ± 0.1 mJy. Under the assumption that there are random 3 hr flares producing both enhanced X-ray and near-infrared emission, our highest limit constrains the variable state of Sgr A* to ,0.8 mJy (observed) or 19 mJy (dereddened). These results suggest that the early model favored by Markoff et al. (2002), in which the flare is produced through local heating of relativistic particles surrounding Sgr A* (e.g., a sudden magnetic reconnection event), is unlikely because it predicts peak 2 ,m emission of ,300 mJy, well above our detection limit. [source] A Relativistic Disk in Sagittarius A*ASTRONOMISCHE NACHRICHTEN, Issue S1 2003Siming Liu The detection of a mm/Sub-mm "bump" in Sgr A*'s radio spectrum suggests that at least a portion of its overall emission is produced within a compact accretion disk. This inference is strengthened by observations of strong linear polarization (at the 10 percent level) within this bump. No linear polarization has been detected yet at other wavelengths. Given that radiation from this source is produced on progressively smaller spatial scales with increasing frequency, the mm/Sub-mm bump apparently arises within a mere handful of Schwarzschild radii of the black hole. We have found that a small (10-Schwarzschild-radii) magnetized accretion disk can not only account for the spectral bump via thermal synchrotron processes, but that it can also reproduce the corresponding polarimetric results. In addition, the quiescent X-ray emission appears to be associated with synchrotron self-Comptonization, while X-ray flares detected from Sgr A* may be induced by a sudden enhancement of accretion through this disk. The hardening of the flare-state X-ray spectrum appears to favor thermal bremsstrahlung as the dominant X-ray emission mechanism during the transient event. This picture predicts correlations among the mm, IR, and X-ray flux densities, that appear to be consistent with recent multi-wavelength observations. Further evidence for such a disk in Sgr A* is provided by its radio variability. Recent monitoring of Sgr A* at cm and mm wavelengths suggests that a spectral break is manifested at 3 mm during cm/Sub-mm flares. The flat cm spectrum, combined with a weak X-ray flux in the quiescent state, rules out models in which the radio emission is produced by thermal synchrotron process in a bounded plasma. One possibility is that nonthermal particles may be produced when the large scale quasi-spherical inflow circularizes and settles down into the small accretion disk. Dissipation of kinetic energy associated with radial motion may lead to particle acceleration in shocks or via magnetic reconnection. On the other hand, the identification of a 106-day cycle in Sgr A*'s radio variability may signal a precession of the disk around a spinning black hole. The disk's characteristics imply rigid-body rotation, so the long precession period is indicative of a small black-hole spin with a spin parameter a/M around 0.1. It is interesting to note that such a small value of a/M would be favored if the nonthermal portion of Sgr A*'s spectrum is powered by a Blandford- Znajek type of process; in this situation, the observed luminosity would correspond to an outer disk radius of about 30 Schwarzschild radii. This disk structure is consistent with earlier hydrodynamical and recent MHD simulations and is implied by Sgr A*'s mm/Sub-mm spectral and polarimetric characteristics. For the disk to precess with such a long (106-day) period, the angular momentum flux flowing through it must be sufficiently small that any modulation of the total angular momentum is mostly due to its coupling with the black-hole spin. This requires that the torque exerted on the inner boundary of the disk via magnetic stresses is close to the angular momentum accretion rate associated with the infalling gas. Significant heating at the inner edge of the disk then leaves the gas marginally bounded near the black hole. A strong wind from the central region may ensue and produce a scaled down version of relativistic (possibly magnetized) jets in AGNs. [source] The Expression Profile of Myogenic Transcription Factors in Satellite Cells from Denervated Rat MuscleBRAIN PATHOLOGY, Issue 2 2002Annette Maier; The muscle-specific transcription factors of the MyoD family are altered after denervation. In order to determine whether this shift takes place in satellite cells (SC), we investigated the expression pattern of MyoD, myf5, myogenin, and MRF4 in SC. Hindlimb muscles of rats were denervated for 2 days to 4 weeks. SC were isolated, pooled and the transcription of all 4 factors was assessed by RT-PCR. Protein expression was assessed in histological sections of soleus and anterior tibial (TA) muscles; SC were identified by M-cadherin. Pooled SC from innervated muscles expressed myf5 mRNA, and very weakly MyoD and myogenin mRNA. MyoD and myogenin protein was found in only few SC. After denervation, pooled SC expressed myf5 mRNA, and very weakly myogenin and MRF4 mRNA. Myogenin protein was found in less than about 10% of the cells, whereas MRF4 protein was absent from SC. We conclude that the presence of myf5 and the absence of MyoD and MRF4 protein in SC after denervation indicated the quiescent state of the cell cycle. A subset of SC has additionally acquired myogenin. SC after denervation might be less easily recruited into the mitotic cycle than SC from normal muscle, rendering regeneration of denervated muscle less efficient than normal muscle. [source] | ||||||||||||||||