			Home About us Contact

Procedure Makes (procedure + make)

Distribution by Scientific Domains

Medical Sciences	40%
Life Sciences	40%

Selected Abstracts

A simple cultural method for the presumptive detection of the yeasts Brettanomyces/Dekkera in wines

LETTERS IN APPLIED MICROBIOLOGY, Issue 6 2005
J.A. Couto
Abstract Aims:, The development of a simple and reliable procedure, compatible with routine use in wineries, for the presumptive detection of Brettanomyces/Dekkera from wine and wine-environment samples. Methods and Results:, The method of detection of these yeasts employs a selective enrichment medium. The medium contains glucose (10 g l,1) as carbon and energy source, cycloheximide (20 mg l,1) to prevent growth of Saccharomyces, chloramphenicol (200 mg l,1) to prevent growth of bacteria and p -coumaric acid (20 mg l,1) as the precursor for the production of 4-ethyl-phenol. After the inoculation with wine, the medium is monitored by visual inspection of turbidity and by periodic olfactive analysis. Contaminated wines will develop visible turbidity in the medium and will produce the 4-ethyl-phenol off-odour, which can be easily detected by smelling. Conclusions:, A selective enrichment liquid medium was developed to differentially promote the growth and activity of Brettanomyces/Dekkera. The method is simple to execute, employing a simple-to-prepare medium and a periodic olfactive detection. Significance and Impact of the Study:, The characteristics of the procedure make it particularly applicable in a wine-making environment thus presenting important advantages to the wine industry. [source]

Application for regenerative medicine of epithelial cell culture-vistas of cultured epithelium

CONGENITAL ANOMALIES, Issue 3 2006
Hajime Inoue
ABSTRACT This review describes culture techniques for the epithelial system as well as trends in the clinical application of cultured keratinocytes in our department and the possibility of applying the techniques to other organs. Cultured epithelium and cultured dermis in particular have considerably preceded regeneration of other organs in the field of regenerative medicine. Since 1988 we have grafted cultured keratinocytes by the Rheinwald-Green modified method in at least 500 patients with large skin defects. As a result of the establishment of a culture technique for individual patients, it is now possible to prepare enough regenerated epithelium to cover the body surface area of as many as 10 adult patients in approximately three weeks after collecting 1 cm2 of skin, and then remaining cultured keratinocytes can be cryo-preserved for two-stage dermatoplasty at another site. This procedure makes it possible to avoid frequent skin collection from the same patient and thereby improves patients' quality of life and activities of daily living. On the other hand, to solve the problem of regenerated epithelium shrinking and problems with graft efficiency on dermis defect lesion, we have developed a proteinase-resistant regenerated dermis by mixing a certain protein with a fibrin scaffold. Recently we also took the initiative in grafting hybrid-type regenerated trachea in an animal experiment by using the epithelial and dermal cell culture technique, and some results of the graft were obtained. [source]

A simple displacement control technique for pushover analyses

EARTHQUAKE ENGINEERING AND STRUCTURAL DYNAMICS, Issue 7 2006
Armelle Anthoine
Abstract This paper explains how to control in displacement any force proportional loading. Such a procedure makes it possible to derive the complete (i.e. including the possible softening branch) response curve of a structure along any radial loading path in the force space. This is exactly what is required in the so-called pushover analysis used in the seismic assessment of structures. The proposed procedure is simple in the sense that it can be easily implemented in any classical (displacement-based) finite element code through a standard displacement control loading process. Furthermore, it leads to an interesting definition of the controlled degree-of-freedom, which, in the case of the pushover analysis, could substitute the classical roof displacement. Copyright © 2006 John Wiley & Sons, Ltd. [source]

Successful Salvage of Kidney Allografts Threatened by Ureteral Stricture Using Pyelovesical Bypass

AMERICAN JOURNAL OF TRANSPLANTATION, Issue 6 2010
R. A. Azhar
Ureteral stricture is the most common urologic complication after renal transplantation. When endourologic management fails, open ureteral reconstruction remains the standard treatment. The complexity of some of these procedures makes it necessary to explore other means of repair. This study evaluated the intermediate-term outcome of subcutaneous pyelovesical bypass graft (SPBG) on renal transplant recipients. We reviewed 8 patients (6 male and 2 female; mean age 52 years) with refractory ureteral strictures postrenal transplantation, who received SPBG as salvage therapy. All patients failed endourologic management and half failed open management of their strictures. After a mean follow-up of 19.4 months, 7 out of 8 renal grafts have good function with mean GFR of 58.5 mL/min/1.73 m2, without evidence of obstruction or infection. One patient lost his graft due to persistent infection of the SPBG and one patient developed a recurrent urinary tract infection managed with long-term antibiotics. SPBG offers a last resort in the treatment of ureteral stricture after renal transplantation refractory to conventional therapy. [source]

Experimental model for analyzing cutting resistance by various knives for cataract surgery

CLINICAL & EXPERIMENTAL OPHTHALMOLOGY, Issue 3 2010
Takeshi Ide MD PhD
Abstract Background:, The trend in current cataract surgery towards clear corneal incision and sutureless procedures makes us realize the importance of wound construction. For optimal surgical outcomes, we need good surgical instruments. In this study, we employed a resistance recording system to analyze the characteristics of seven commercially available disposable cataract knives and to find clues for the future development of ,good' cataract knives. Methods:, The cutting resistance was recorded during perpendicular penetrations of porcine scleral tissues by cataract knives. This data was processed and analysed mathematically with MATLAB software (The MathWorks, Inc, Natick, MA, USA) to see the resistance wave shapes and their derivatives to show the products' differing characteristics. Results:, The wave shapes demonstrated product-dependent characteristics. The average maximum penetration resistance varied from 86.4 to 233 mN. The first order time derivatives also showed distinctive wave shapes. Conclusion:, We used an experimental model to analyze one aspect of a knife's character. This model can help give clues for future developments, although this is the initial step. [source]