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Progesterone Metabolite (progesterone + metabolite)

Distribution by Scientific Domains

Medical Sciences	100%

Selected Abstracts

Measurement of Faecal Progesterone Metabolites and its Application for Early Screening of Open Cows Post-insemination

REPRODUCTION IN DOMESTIC ANIMALS, Issue 3 2007
B Kornmatitsuk
Contents The present study investigated the changes of serum progesterone (P4) and its faecal metabolites in pregnant and non-pregnant cows (Expt 1) and the feasibilty of using faecal P4 metabolites for early screening of open cows post-insemination (Expt 2). In Expt 1, seven crossbred Holstein,Friesian (HF) cows were studied. Serum and faecal samples were collected once daily from the day of artificial insemination (AI) until 25 days after AI. In Expt 2, 27 crossbred HF inseminated cows were employed. Serum and faecal samples were obtained on the day of AI (day 0) and on days 19,22 post-insemination. Enzyme immunoassay measurements of serum P4 and faecal P4 metabolites were established. The low detection limit of the assay was 0.01 ng/ml and the amount of P4, resulting in a 50% reduction in the initial binding value, was 1.07 ng/ml. The intra- and inter-assay coefficients of variation were <8% and <14%, respectively. A positive correlation between the levels of serum P4 and faecal P4 metabolites was found in every single cow (r = 0.73,0.88, p < 0.001) and pooled data (r = 0.78, p < 0.001). The estimated value of faecal P4 metabolites at 100 ng/g of faeces was equal to the serum P4 levels of 1 ng/ml. The accuracies of pregnancy and non-pregnancy diagnosis based on the analyses of faecal P4 metabolites between day 0 and days 19,22 post-insemination, were 67% and 100%, respectively. In conclusion, the measurement of faecal P4 metabolites can be a potentially alternative method for early screening of open cows post-insemination with the same accuracy and precision, as measured by serum P4 assay. [source]

The effects of a progesterone metabolite, 5,-dihydroprogesterone, on oxytocin receptor binding in human myometrial membranes

BJOG : AN INTERNATIONAL JOURNAL OF OBSTETRICS & GYNAECOLOGY, Issue 6 2003
Shirley Astle
Objective To determine the effect of the progesterone metabolite 5,-dihydroprogesterone on human oxytocin receptor binding in myometrial membranes and on whole-cell calcium current in single myometrial cells. Design Receptor binding studies in human myometrial membranes prepared from biopsies taken before or after the onset of labour and in Chinese hamster ovary cells expressing the human oxytocin receptor. Whole cell patch-clamp experiments were undertaken on isolated myometrial cells. Setting University research laboratories and University hospital. Population Patients undergoing caesarean section at term either prior to or following onset of labour. Methods Myometrial biopsies were taken from women undergoing caesarean section. The binding affinities of oxytocin, 5,-dihydroprogesterone and atosiban were determined in myometrial membranes and Chinese hamster ovary cells expressing the human oxytocin receptor. The effect of 5,-dihydroprogesterone on inward current was also determined in isolated myometrial cells. Main outcome measures Receptor binding affinity and electrophysiological inward current. Results 5,-Dihydroprogesterone did not reduce oxytocin receptor binding in myometrial membranes or Chinese hamster ovary cells expressing the human oxytocin receptor. Nor did it influence calcium current under whole-cell patch conditions in single myometrial cells. In contrast, atosiban inhibited binding in myometrial membranes prepared from samples taken either prior to or following labour (Ki= 112 and 108 nM, respectively). The affinity of atosiban for the oxytocin receptor was much lower than oxytocin (Ki= 5 and 6 nM in samples taken before or after labour, respectively) in myometrial membranes and in Chinese hamster ovary cells expressing the human oxytocin receptor (Ki= 63 M and 1 nM for atosiban and oxytocin, respectively). Conclusions We conclude that 5,-dihydroprogesterone is unlikely to regulate myometrial activity as a result of a direct effect on oxytocin receptor binding or inward calcium current. [source]

Neuroactive steroids and fatigue severity in patients with primary biliary cirrhosis and hepatitis C

NEUROGASTROENTEROLOGY & MOTILITY, Issue 6 2008
S. Ahboucha
Abstract, Fatigue is one of the most common non-specific symptoms associated with several disease states including liver diseases. Recently, it was reported that levels of progesterone metabolites such as allopregnanolone (3,,5,-tetrahydroprogesterone; 3,,5,-THP) and isopregnanolone (3,,5,-THP) were increased in plasma of patients with chronic fatigue syndrome. We hypothesize that THP metabolites might be associated with fatigue commonly observed in chronic liver diseases. We evaluated fatigue scores and plasma levels of five progesterone metabolites in 16 patients with primary biliary cirrhosis (PBC), 12 patients with chronic hepatitis C (CHC) and 11 age-matched controls. The fatigue impact scale (FIS) ratio was significantly increased (P < 0.01) in patients with PBC and CHC compared to controls. Plasma levels of 3,,5,-THP and pregnanolone (3,,5,-THP) were significantly increased in PBC and CHC patients. The other progesterone metabolites, i.e. 3,,5,-THP, 3,,5,-THP and 3,,5,-tetrahydrodeoxycorticosterone were either undetectable or detected only in some patients. Plasma levels of 3,,5,-THP and 3,,5,-THP were found to be significantly higher in patients with fatigue (P < 0.05), while those of patients without fatigue were not significantly different from controls. Both 3,,5,-THP and 3,,5,-THP are positive allosteric modulators of the gamma-aminobutyric acid type A (GABA-A) receptor and readily cross the blood,brain barrier. The present preliminary findings suggest that increased inhibition through GABA-A receptors due to the accumulation of neuroinhibitory steroids may represent an important pathophysiological mechanism of fatigue in chronic liver diseases. [source]

Chronological aspects of ultrasonic, hormonal, and other indirect indices of ovulation

BJOG : AN INTERNATIONAL JOURNAL OF OBSTETRICS & GYNAECOLOGY, Issue 8 2001
René Ecochard
Objective To improve prediction of ovulation in normal cycles. Design Collection of women's characteristics and their menstrual cycles. Monitoring and analysis of time relationships between several indicators of ovulation: transvaginal ultrasonography, cervical mucus, basal body temperature, urinary luteinising hormone, and ratio of urinary oestrogen to progesterone metabolites. Setting Each of eight natural family planning clinics was to study 12 women for at least three cycles. Population One hundred and seven normally fertile and cycling women aged 18 to 45. Methods Daily measurements of urinary luteinising hormone, follicle stimulating hormone, oestrone-3-glucuronide and pregnanediol-3, -glucuronide. Basal body temperature recording and cervical mucus checking. Transvaginal ultrasound examination of the ovaries. Main outcome measures Delays between the expected day of ovulation according to the luteinising hormone peak or to ultrasound evidence and the expected days according to the other indices of ovulation. Results Ultrasonography was able to show evidence of ovulation in 283 out of 326 cycles. The average time lag between luteinising hormone peak and ultrasound evidence was less than one day (+0.46) but premature and late luteinising hormone-expected date of ovulation were observed in nearly 10% and 23% of cycles, respectively. Basal body temperature rise was observed in 98% of cycles. Cervical mucus peak symptom, rapid drop in the ratio of urinary metabolites, and luteinising hormone initial rise were all close to ultrasonographic evidence in more than 72% of cycles. Conclusions For accuracy and practical reasons, the cervical mucus peak symptom, the ratio of urinary metabolites and luteinising hormone initial rise might be better indices of ovulation than the luteinising hormone peak. [source]