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Porcine Reproductive (porcine + reproductive)
Selected AbstractsExotic Animal Diseases Bulletin January 2000 No. 72 Update on Porcine reproductive and respiratory syndromeAUSTRALIAN VETERINARY JOURNAL, Issue 1 2000Article first published online: 10 MAR 200 The Exotic Animal Disease Bulletin is produced by Agriculture, Fisheries and Forestry-Australia (AFFA). For further information, contact the Animal Health Science and Emergency Management Branch, National Office of Animal and Plant Health, AFFA, GPO Box 858, Canberra ACT 2601. [Phone: (02) 6272 4509; fax: (02) 6272 3372; e-mail: neil.tweddle@affa.gov.au] [source] Mouse × pig chimeric antibodies expressed in Baculovirus retain the same properties of their parent antibodiesBIOTECHNOLOGY PROGRESS, Issue 2 2009Ana M. Jar Abstract The development of hybridoma and recombinant DNA technologies has made it possible to use antibodies against cancer, autoimmune disorders, and infectious diseases in humans. These advances in therapy, as well as immunoprophylaxis, could also make it possible to use these technologies in agricultural species of economic importance such as pigs. Porcine reproductive and respiratory syndrome virus (PRRSV) is an arterivirus causing very important economic losses to the industry. Passive transfer of antibodies obtained by biotechnology could be used in the future to complement or replace vaccination against this and other pig pathogens. To this end, we constructed and studied the properties of chimeric mouse × pig anti-PRRSV antibodies. We cloned the constant regions of gamma-1 and gamma-2 heavy chains and the lambda light chain of pig antibodies in frame with the variable regions of heavy and light chains of mouse monoclonal antibody ISU25C1, which has neutralizing activity against PRRSV. The coding regions for chimeric IgG1 and IgG2 were expressed in a baculovirus expression system. Both chimeric antibodies recognized PRRSV in ELISA as well as in a Western-blot format and, more importantly, were able to neutralize PRRSV in the same fashion as the parent mouse monoclonal antibody ISU25C1. In addition, we show that both pig IgG1 and IgG2 antibodies could bind complement component C1q, with IgG2 being more efficient than IgG1 in binding C1q. Expressing chimeric pig antibodies with protective capabilities offers a new alternative strategy for infectious disease control in domestic pigs. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009 [source] QTL for traits related to humoral immune response estimated from data of a porcine F2 resource populationINTERNATIONAL JOURNAL OF IMMUNOGENETICS, Issue 3 2009K. Wimmers Summary This study aimed to map quantitative trait loci (QTL) for traits related to humoral innate immune defence. Therefore, haemolytic complement activity in the alternative and the classical pathway, serum concentration of C3c and of haptoglobin (HP) were measured in blood samples obtained from F2 piglets (n = 457) of a porcine F2 resource population before and after Mycoplasma hyopneumoniae, Aujeszky's disease virus (Suid herpesvirus I, SuHVI) and porcine reproductive and respiratory syndrome virus (PRRSV) vaccination at 6, 14 and 16 weeks of age. Animals were genotyped at 88 autosomal markers. QTL analysis was performed under the line cross and the half sib. Phenotypic data were adjusted for systematic effects by mixed models with and without repeated measures statement. In total, 46 and 21 estimated QTL positions were detected with genome-wide significance at the 0.05 and 0.01 level, respectively. The proximal region of SSC2 (orthologous to HSA11 0,70 Mb), the distal region of SSC4 (HSA1 95,155 Mb), and the intermediate region of SSC16 (HSA5 0,73 Mb and 150,174 Mb) showed a clustering of estimated QTL positions for complement activity based on the different models. A common genetic background, i.e. a single true QTL, might underlie these QTL positions for related traits. In addition, QTL for antibody titres were detected on SSC1, 2, 6 and 7. With regard to number and magnitude of their impact, QTL for humoral innate immune traits behave like those for other quantitative traits. Discovery of such QTL facilitates the identification of candidate genes for disease resistance and immune competence that are applicable in selective breeding and further research towards improving therapeutic and prophylactic measures. [source] Influence of dietary ß-glucan on growth performance, lymphocyte proliferation, specific immune response and haptoglobin plasma concentrations in pigsJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 1-2 2003S. Hiss Summary Immunomodulatory feed additives might offer alternatives to anti-microbial growth promoters in swine production. The present study was conducted to assess the effects of ,-1,3/1,6 glucans, i.e. of specific yeast cell wall components, on immune function and growth performance in pigs. After weaning at 4 weeks of age, 75 piglets were allocated to 3 different groups for 4 weeks, the diet was supplemented with 0, 0.015 or 0.03% of ,-glucan, respectively. All animals were vaccinated against porcine reproductive and respiratory syndrome (PRRS). After 4 weeks, average daily gains (ADG) of ,-glucan treated pigs were not different from the controls. Feed intake was tendentiously (p < 0.1) increased at 0.03%,-glucan, without alteration of feed efficiency. Serum haptoglobin concentrations at the end of the 4 week treatment were increased in all groups when compared to the initial levels (p < 0.001), without differences between the groups (p > 0.05). Haptoglobin levels were inversely related to ADG. Lymphocyte proliferation indices were not different in control and treatment groups. Specific vaccination responses, as quantified by the PRRS antibody titres occurred in all animals, but no relation with ,-glucan feeding was observed. Our results indicate marginal benefits of ,-glucan supplements for growth performance and no effect on the immune parameters tested. The observed trend towards increased feed intake needs further elucidation. [source] Prevalence of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in piglets after weaning on a commercial pig farm in JapanANIMAL SCIENCE JOURNAL, Issue 1 2010Kenichi SASAKI ABSTRACT To investigate the transition in concentration of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) and antibody for these viruses in serum, serum samples were collected from 29 pigs on weaning day and at 7, 14, 21, 28, 53, 84, and 120 days after weaning. The concentration of circulated PRRSV and PCV2 in serum was measured by real-time RT-PCR and real-time PCR, respectively. The specific antibody for PRRSV and PCV2 was measured using ELISA. PRRSV was not detected on 0 days post-weaning (dpw). The specific antibody for PRRSV began to increase as the concentration of PRRSV in serum increased, and the level of PRRSV then tended to decrease. PCV2 was detected in 12 of 28 pigs on 0 dpw. The concentration of PCV2 and the specific antibody for PCV2 showed a similar tendency to those of PRRSV. The correlation analysis suggests that a decline in the daily weight gain coincided with an increase in the PRRSV concentration. Pigs with a higher antibody titer against PRRSV or PCV2 on 0 dpw showed the lower level of PRRSV or PCV2, respectively. [source] | |||||||||||||