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Polymorphic Microsatellite DNA Markers (polymorphic + microsatellite_dna_marker)
Selected AbstractsPolymorphic microsatellite DNA markers in the Asiatic black bear Ursus thibetanusMOLECULAR ECOLOGY, Issue 10 2000E. Kitahara [source] Polymorphic microsatellite DNA markers for the marine gastropod Littorina subrotundataMOLECULAR ECOLOGY, Issue 1 2000A. D. Tie [source] Polymorphic microsatellite DNA markers in the mudflat topshell Diloma subrostrata (Gastropoda, Trochidae)MOLECULAR ECOLOGY RESOURCES, Issue 3 2006K. M. DONALD Abstract Very little information is available on the intraspecific genetic structure of topshell populations. Here, we report the characterization of five polymorphic microsatellite loci in the New Zealand mudflat topshell, Diloma subrostrata. The number of alleles per locus ranged from two to 23, observed and expected heterozygosities did not deviate from Hardy,Weinberg equilibrium (P < 0.05) and no linkage disequilibrium was detected between locus pairs (P < 0.05). We are currently using these markers to investigate genetic population structure of D. subrostrata in New Zealand. [source] Polymorphic microsatellite DNA markers for the white-breasted thrasher, Ramphocinclus brachyurusMOLECULAR ECOLOGY RESOURCES, Issue 3 2006LI JIN Abstract We isolated and characterized six polymorphic microsatellite markers for the white-breasted thrasher from genomic libraries enriched for (AC)n, (GT)n, (CAAA)n, (TTTC)n, (GAC)n, (CT)n and (TTTG)n microsatellites. The number of alleles per locus ranged from two to seven. Observed heterozygosity (HO) ranged from 0.30 to 0.85. [source] Polymorphic microsatellite DNA markers for the grey fantail, Rhipidura albiscapaMOLECULAR ECOLOGY RESOURCES, Issue 1 2006L. JIN Abstract We isolated and characterized five polymorphic microsatellite markers for the grey fantail Rhipidura albiscapa from genomic libraries enriched for (AC)n and (GT)n microsatellites. In 34 adult individuals, the number of alleles per locus ranged from eight to 17. Observed and expected heterozygosities ranged from 0.65 to 0.94 and 0.83 to 0.94, respectively. These markers will be useful for analysing extra-pair paternity in fantails. [source] Polymorphic microsatellite DNA markers for the Patagonian squid, Loligo gahi (Cephalopoda)MOLECULAR ECOLOGY RESOURCES, Issue 3 2002P. W. Shaw Abstract Squid populations are being put under rapidly increasing commercial fishing pressure worldwide. The same species are known to be susceptible to extreme population fluctuations, so detailed knowledge of population substructuring and genetic diversity is essential for rational management. We present a set of microsatellite DNA loci suitable for population genetic analysis of Loligo gahi, the squid species subject to the most detailed monitoring and fishery control (around the Falkland Islands), with the future aim of generating management-related information to aid conservation of this valuable natural resource. [source] Polymorphic microsatellite DNA markers in the ant Formica exsectaMOLECULAR ECOLOGY RESOURCES, Issue 1 2002Niclas Gyllenstrand Abstract Highly polymorphic genetic markers provide a useful tool for estimating important genetic parameters in studies of the evolution of sociality in insects. Here we report 14 polymorphic microsatellite markers developed in the ant Formica exsecta. The number of alleles found ranged between 3 and 18 per locus. These markers were developed for studying genetic population structure and mating structure in F. exsecta populations with varying social organizations (monogyne and polygyne types of societies). Cross-species amplification indicated that some of the markers might be usable even in species belonging to different subfamilies. [source] Microsatellite DNA markers reveal genetic differentiation among populations of Glossina palpalis gambiensis collected in the agro-pastoral zone of Sideradougou, Burkina FasoINSECT MOLECULAR BIOLOGY, Issue 4 2000P. Solano Abstract Intraspecific genetic variability of Glossina palpalis gambiensis in the area of Sideradougou, Burkina Faso, was studied using polymorphic microsatellite DNA markers. This genetic study was combined with other epidemiological information on the same tsetse: bloodmeal identification, dissection of tsetse and molecular characterization of the trypanosomes detected. There was significant genetic differentiation among flies caught only a few kilometers apart, within the same riverine habitat. These distinct subpopulations were also differentially infected by trypanosomes. In part of the study area, a Factorial Correspondence Analysis undertaken on the genotypes allowed us to detect a Wahlund effect, suggesting the presence of tsetse originating from different source populations coming from two distinct drainage systems. The apparent structuring of populations of G. palpalis gambiensis is discussed relative to appropriate strategies to control African Trypanosomosis. [source] Isolation and characterization of microsatellite loci in the whale shark (Rhincodon typus)MOLECULAR ECOLOGY RESOURCES, Issue 3 2009DENÍ RAMÍREZ-MACÍAS Abstract In preparation for a study on population structure of the whale shark (Rhincodon typus), nine species-specific polymorphic microsatellite DNA markers were developed. An initial screening of 50 individuals from Holbox Island, Mexico found all nine loci to be polymorphic, with two to 17 alleles observed per locus. Observed and expected heterozygosity per locus ranged from 0.200 to 0.826 and from 0.213 to 0.857, respectively. Neither statistically significant deviations from Hardy,Weinberg expectations nor statistically significant linkage disequilibrium between loci were observed. These microsatellite loci appear suitable for examining population structure, kinship assessment and other applications. [source] Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigasMOLECULAR ECOLOGY RESOURCES, Issue 3 2009C. SAUVAGE Abstract We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-Oléron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance. [source] Microsatellite DNA markers for three toad-headed lizard species (Phrynocephalus vlangalii, P. przewalskii and P. guttatus)MOLECULAR ECOLOGY RESOURCES, Issue 2 2009AIBIN ZHAN Abstract To assess the impact of natural landscapes on the population structure of lizards, 10 polymorphic microsatellite DNA markers were developed for the Qinghai toad-headed lizard, Phrynocephalus vlangalii. The number of alleles at these informative loci ranged from four to 28. The novel markers and those previously developed for Phrynocephalus przewalskii were cross-tested among three toad-headed lizard species P. vlangalii, P. przewalskii and P. guttatus. A high cross-utility rate of more than 58% was observed among these three species. These markers are expected to be useful tools for taxonomic considerations as well as population genetic analysis and future conservation management. [source] Development of 18 polymorphic microsatellite DNA markers of Laminaria japonica (Phaeophyceae)MOLECULAR ECOLOGY RESOURCES, Issue 4 2007YUANYUAN SHI Abstract Eighteen polymorphic microsatellite DNA markers were developed for Laminaria japonica, a brown alga cultured intensively in China. These markers are independent from each other and are moderately variable in L. japonica. The number of alleles and gene diversity detected in 53 gametophyte clones representing the varieties of L. japonica cultured once or currently in China range from two to nine and from 0.355 to 0.768, respectively. These markers will certainly facilitate the management and exploitation of the germplasm resource of L. japonica conserved indoor as gametophyte clones and the determination of the genetic diversity of L. japonica naturally distributed. [source] Isolation and characterization of polymorphic microsatellite DNA markers in the brown sole, Pleuronectes herzensteiniMOLECULAR ECOLOGY RESOURCES, Issue 1 2007S. G. KIM Abstract New microsatellite DNA markers from brown sole were developed and characterized. Fourteen primer sets were designed from 40 microsatellite regions. Eight of 14 loci exhibited variations comprising 8,31 alleles. Observed and expected heterozygosities ranged from 0.611 to 0.833 and from 0.647 to 0.968 among 36 individuals, respectively. Phz3, Phz8 and Phz12 significantly deviated from Hardy,Weinberg equilibrium, and there was a significant linkage disequilibrium between Phz2 and Phz12. Seven of eight loci conformed to the Mendelian manner of inheritance in a full-sib family. Seven to four loci of three related species were cross-amplified by primers for brown sole. [source] Isolation and characterization of 43 microsatellite DNA markers for guppy (Poecilia reticulata)MOLECULAR ECOLOGY RESOURCES, Issue 4 2003T. Watanabe Abstract Thirty-nine polymorphic microsatellite DNA markers were isolated from the guppy (Poecilia reticulata) genomic library. All of the loci showed moderate allelic variation ranging from two to seven alleles, with observed heterozygosities from 0.000 to 0.938. The microsatellite DNA markers isolated will be available for use in analysis of quantitative trait loci in breeding programmes and for population genetic studies on experimental fish. [source] | ||||||||||