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Powdery Mildew Resistance Genes (powdery + mildew_resistance_gene)
Selected AbstractsIdentification and Mapping of Two New Genes Conferring Resistance to Powdery Mildew from Aegilops tauschii (Coss.) SchmalJOURNAL OF INTEGRATIVE PLANT BIOLOGY, Issue 10 2006Xiao-Li Sun Abstract Two powdery mildew resistance genes were identified from Aegilops tauschii accessions Y201 and Y212 and mapped using two different F2 populations derived from the crosses between susceptible accession Y2272 and Y201, and susceptible accession Y2263 and Y212. Genetic analysis of resistance to powdery mildew indicated that the resistance of Y201 was controlled by a single dominant gene, whereas the resistance of Y212 was controlled by a single recessive gene. We have temporarily designated these genes as PmY201 and PmY212, respectively. By bulk segregation analysis, six microsatellite markers including Xgwm174, cfd26, cfd57, cfd102, Xgwm583 and Xgwm639 were found to be linked to PmY201 with genetic distances of 5.2, 7.7, 9.6, 12.5, 20.2 and 22.1 cM, respectively. Five SSR markers, including cfd57, Xgwm182, cfd7, cfd102, and cfd12, were found to be linked to PmY212 with distances of 5.6, 7.2, 11.5, 14.7, and 18.5 cM, respectively. According to the locations of the linked markers, the two resistance genes were located in the 5DL region. Based on the chromosomal locations and the resistance patterns of the two genes, we propose that PmY201 and PmY212 are two novel powdery mildew resistance genes, and are suitable for marker-assisted selection. (Managing editor: Ya-Qin Han) [source] Natural genetic resources of Arabidopsis thaliana reveal a high prevalence and unexpected phenotypic plasticity of RPW8- mediated powdery mildew resistanceNEW PHYTOLOGIST, Issue 3 2008Katharina Göllner Summary ,,Here, an approach based on natural genetic variation was adopted to analyse powdery mildew resistance in Arabidopsis thaliana. ,,Accessions resistant to multiple powdery mildew species were crossed with the susceptible Col-0 ecotype and inheritance of resistance was analysed. Histochemical staining was used to visualize archetypal plant defence responses such as callose deposition, hydrogen peroxide accumulation and host cell death in a subset of these ecotypes. ,,In six accessions, resistance was likely of polygenic origin while 10 accessions exhibited evidence for a single recessively or semi-dominantly inherited resistance locus. Resistance in the latter accessions was mainly manifested at the terminal stage of the fungal life cycle by a failure of abundant conidiophore production. The resistance locus of several of these ecotypes was mapped to a genomic region containing the previously analysed atypical RPW8 powdery mildew resistance genes. Gene silencing revealed that members of the RPW8 locus were responsible for resistance to Golovinomyces orontii in seven accessions. ,,These results suggest that broad-spectrum powdery mildew resistance in A. thaliana is predominantly of polygenic origin or based on RPW8 function. The findings shed new light on the natural variation of inheritance, phenotypic expression and pathogen range of RPW8 -conditioned powdery mildew resistance. [source] Powdery mildew resistance in Czech and Slovak barley cultivarsPLANT BREEDING, Issue 3 2000A. Dreiseitl Abstract Fifteen powdery mildew resistance genes and the gene MlaN81 derived from ,Nepal 81'were found in 76 Czech and Slovak spring and winter barley cultivars when tested for reaction to a set of powdery mildew isolates. Nine cultivars (,Donum', ,Expres', ,Jubilant', ,Orbit', ,Primus', ,Progres', ,Stabil', ,Vladan' and ,Zlatan') are composed of lines with different resistance genes. The Mlat gene is present in nine cultivars and was transferred from the Anatolian landrace ,A-516,. The resistances derived from ,KM-1192'and ,CI 7672'were identical and designated Ml(Kr). Five winter barley cultivars possess the Ml(Bw) resistance. The winter barley line ,KM-2099'carries the mlo gene. The parental cultivar ,Palestine 10'was also tested in which the genes Mlk1, MlLa were identified. The German cultivar ,Salome', a parent of seven cultivars tested, probably carries the gene MlLa in addition to mlo and Mla7. The gene mlo6 may be present in the cultivar ,Heris'. Most of the results were confirmed by the pedigrees of the cultivars. [source] Mapping of powdery mildew resistance genes in a newly determined accession of Hordeum vulgare ssp. spontaneumANNALS OF APPLIED BIOLOGY, Issue 2 2010K. Teturová The accession PI466197 of wild barley (Hordeum vulgare ssp. spontaneum) with a newly identified resistance to powdery mildew caused by Blumeria graminis f.sp. hordei was studied with the aim to localise the genes determining resistance on a barley genetic map using DNA markers. Molecular analysis was performed in the F2 population of the cross between the winter variety ,Tiffany' and the resistant accession PI466197, consisting of 113 plants. DNA markers, 17 simple sequence repeats (SSRs), four sequence-tagged sites (STSs) and one cleaved amplified polymorphic sequence (CAPS) marker developed from the Mla locus sequence were used for genetic mapping and a two-locus model of resistance was shown. One of the resistance genes originating from H. vulgare ssp. spontaneum PI466197 was localised between the markers RGH1aE1 and Bmac0213 on the short arm of chromosome 1H, which is the position consistent with the Mla locus. The other gene was proven to be highly significantly linked with GBMS247, Bmac0134 and MWG878 on the short arm of chromosome 2H. The flanking markers were Bmac0134 and MWG878, assigned 4 and 8 cM from the resistance gene, respectively. Until now, no gene conferring powdery mildew resistance originating from H. vulgare has been located on the short arm of barley chromosome 2H. [source] | ||||||||||